Restriction map for
pF1KSDA0785
(Circular) (Six-base) MAPSORT of: pF1KSDA0785.seq Check: 3187 from: 1 to: 5221
pF1KSDA0785 5221 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1332 1590 2046 2133 1332
Size: 258 456 87 4420
Fragments arranged by size:
4420 456 258 87
AatII G_ACGT'C
Cuts at: 977 977
Size: 5221
AccI GT'mk_AC
Cuts at: 2257 2257
Size: 5221
Acc65I G'GTAC_C
Cuts at: 1370 2235 1370
Size: 865 4356
AclI AA'CG_TT
Cuts at: 5061 5061
Size: 5221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 436 604 700 844 3044 3476 4225 4493
Size: 252 168 96 144 2200 432 749 268
Cuts at: 4493 184
Size: 912
Fragments arranged by size:
2200 912 749 432 268 252 168 144
96
AflIII A'CryG_T
Cuts at: 722 1523 2571 3677 4654 722
Size: 801 1048 1106 977 1289
Fragments arranged by size:
1289 1106 1048 977 801
AgeI A'CCGG_T
Cuts at: 3609 3609
Size: 5221
AleI CACnn'nnGTG
Cuts at: 871 871
Size: 5221
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1213 1245 2131 2163 1213
Size: 32 886 32 4271
Fragments arranged by size:
4271 886 32 32
AlwNI CAG_nnn'CTG
Cuts at: 501 2524 4093 501
Size: 2023 1569 1629
Fragments arranged by size:
2023 1629 1569
ApaLI G'TGCA_C
Cuts at: 982 3991 982
Size: 3009 2212
ApoI r'AATT_y
Cuts at: 346 2223 4574 4830 346
Size: 1877 2351 256 737
Fragments arranged by size:
2351 1877 737 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5221
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5221
AvaI C'yCGr_G
Cuts at: 340 421 439 1513 1696 2101 2239 340
Size: 81 18 1074 183 405 138 3322
Fragments arranged by size:
3322 1074 405 183 138 81 18
BamHI G'GATC_C
Cuts at: 1993 2244 1993
Size: 251 4970
BanI G'GyrC_C
Cuts at: 787 837 1370 1692 2235 2649 2897 2932 787
Size: 50 533 322 543 414 248 35 3076
Fragments arranged by size:
3076 543 533 414 322 248 50 35
BanII G_rGCy'C
Cuts at: 151 566 814 1325 1457 2233 3263 151
Size: 415 248 511 132 776 1030 2109
Fragments arranged by size:
2109 1030 776 511 415 248 132
BbeI G_GCGC'C
Cuts at: 791 2653 2901 791
Size: 1862 248 3111
Fragments arranged by size:
3111 1862 248
BbsI GAAGACnn'nnnn_
Cuts at: 592 832 879 909 1886 592
Size: 240 47 30 977 3927
Fragments arranged by size:
3927 977 240 47 30
BbvCI CC'TCA_GC
Cuts at: 1673 1673
Size: 5221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 930 964 930
Size: 34 5187
BciVI GTATCCnnnnn_n'
Cuts at: 3117 3880 5221 3117
Size: 763 1341 3117
Fragments arranged by size:
3117 1341 763
BclI T'GATC_A
Cuts at: 2739 2739
Size: 5221
BfrBI ATG'CAT
Cuts at: 239 239
Size: 5221
BglI GCCn_nnn'nGGC
Cuts at: 463 1790 463
Size: 1327 3894
BglII A'GATC_T
Cuts at: 1682 1775 2734 4773 1682
Size: 93 959 2039 2130
Fragments arranged by size:
2130 2039 959 93
BlpI GC'TnA_GC
Cuts at: 1456 1840 2331 1456
Size: 384 491 4346
Fragments arranged by size:
4346 491 384
Bme1580I G_kGCm'C
Cuts at: 842 986 1697 2844 2937 3995 842
Size: 144 711 1147 93 1058 2068
Fragments arranged by size:
2068 1147 1058 711 144 93
BmrI ACTGGGnnnn_n'
Cuts at: 2431 2512 2847 4993 2431
Size: 81 335 2146 2659
Fragments arranged by size:
2659 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1461 3586 1461
Size: 2125 3096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 346 1303 1350 163
Size: 183 957 47 4034
Fragments arranged by size:
4034 957 183 47
Bpu10I CC'TnA_GC
Cuts at: 1673 1979 1673
Size: 306 4915
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1201 1873 2395 3274 3768 4066 1201
Size: 672 522 879 494 298 2356
Fragments arranged by size:
2356 879 672 522 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 853 1432 4925 853
Size: 579 3493 1149
Fragments arranged by size:
3493 1149 579
BsaAI yAC'GTr
Cuts at: 2046 2211 2572 2046
Size: 165 361 4695
Fragments arranged by size:
4695 361 165
BsaBI GATnn'nnATC
Cuts at: 6 2733 4772 6
Size: 2727 2039 455
Fragments arranged by size:
2727 2039 455
BsaHI Gr'CG_yC
Cuts at: 788 974 1510 2165 2650 2898 3602 5098 788
Size: 186 536 655 485 248 704 1496 911
Fragments arranged by size:
1496 911 704 655 536 485 248 186
BsaWI w'CCGG_w
Cuts at: 295 2408 2929 3609 3883 4030 4441 295
Size: 2113 521 680 274 147 411 1075
Fragments arranged by size:
2113 1075 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 532 562 1162 1192 1213 1243 2131 2161 532
Size: 30 600 30 21 30 888 30 3592
Fragments arranged by size:
3592 888 600 30 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 744 1714 1849 744
Size: 970 135 4116
Fragments arranged by size:
4116 970 135
BseYI C'CCAG_C
Cuts at: 431 516 543 613 1047 2644 3402 3981 4477
Size: 85 27 70 434 1597 758 579 496
Cuts at: 4477 431
Size: 1175
Fragments arranged by size:
1597 1175 758 579 496 434 85 70
27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 1387
Size: 5221
BsiEI CG_ry'CG
Cuts at: 104 509 593 1669 2761 2807 4017 104
Size: 405 84 1076 1092 46 1210 1308
Fragments arranged by size:
1308 1210 1092 1076 405 84 46
BsiHKAI G_wGCw'C
Cuts at: 151 878 964 986 1195 1325 1457 2233 3011
Size: 727 86 22 209 130 132 776 778
Cuts at: 3011 3201 3995 151
Size: 190 794 1377
Fragments arranged by size:
1377 794 778 776 727 209 190 132
130 86 22
BsmBI CGTCTCn'nnnn_
Cuts at: 1182 1501 1990 1182
Size: 319 489 4413
Fragments arranged by size:
4413 489 319
Bsp1286I G_dGCh'C
Cuts at: 151 566 814 842 878 964 986 1195 1325
Size: 415 248 28 36 86 22 209 130
Cuts at: 1325 1457 1697 2233 2844 2937 3011 3201 3263
Size: 132 240 536 611 93 74 190 62
Cuts at: 3263 3995 151
Size: 732 1377
Fragments arranged by size:
1377 732 611 536 415 248 240 209
190 132 130 93 86 74 62 36
28 22
BspEI T'CCGG_A
Cuts at: 295 4441 295
Size: 4146 1075
BspHI T'CATG_A
Cuts at: 4397 4397
Size: 5221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1332 1590 2046 2133 2269 2483 2785 3166 1332
Size: 258 456 87 136 214 302 381 3387
Fragments arranged by size:
3387 456 381 302 258 214 136 87
BsrBI CCG'CTC
Cuts at: 50 1593 3511 3565 5051 5216 50
Size: 1543 1918 54 1486 165 55
Fragments arranged by size:
1918 1543 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3131 3131
Size: 5221
BsrFI r'CCGG_y
Cuts at: 466 1015 1664 1668 2027 3217 3398 3609 466
Size: 549 649 4 359 1190 181 211 2078
Fragments arranged by size:
2078 1190 649 549 359 211 181 4
BsrGI T'GTAC_A
Cuts at: 1112 1112
Size: 5221
BssSI C'ACGA_G
Cuts at: 3490 3850 3490
Size: 360 4861
BstAPI GCAn_nnn'nTGC
Cuts at: 2596 2596
Size: 5221
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5221
BstXI CCAn_nnnn'nTGG
Cuts at: 1386 1548 1386
Size: 162 5059
BstYI r'GATC_y
Cuts at: 1 1682 1775 1993 2244 2711 2734 3069 3315
Size: 1681 93 218 251 467 23 335 246
Cuts at: 3315 4329 4415 4427 4773 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1681 1014 467 449 346 335 251 246
218 93 86 23 12
BtgI C'CryG_G
Cuts at: 125 471 806 1103 125
Size: 346 335 297 4243
Fragments arranged by size:
4243 346 335 297
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1221 1461 3169 3350 3513 1221
Size: 240 1708 181 163 2929
Fragments arranged by size:
2929 1708 240 181 163
BtsI GCAGTG_nn'
Cuts at: 253 986 1152 2462 253
Size: 733 166 1310 3012
Fragments arranged by size:
3012 1310 733 166
DraI TTT'AAA
Cuts at: 2218 2218
Size: 5221
DraIII CAC_nnn'GTG
Cuts at: 704 704
Size: 5221
DrdI GACnn_nn'nnGTC
Cuts at: 2925 3785 2925
Size: 860 4361
EaeI y'GGCC_r
Cuts at: 455 468 506 590 689 764 1106 1199 1346
Size: 13 38 84 99 75 342 93 147
Cuts at: 1346 1463 1538 1666 1769 1961 2804 2978 3369
Size: 117 75 128 103 192 843 174 391
Cuts at: 3369 3396 4743 455
Size: 27 1347 933
Fragments arranged by size:
1347 933 843 391 342 192 174 147
128 117 103 99 93 84 75 75
38 27 13
EagI C'GGCC_G
Cuts at: 506 590 1666 2804 506
Size: 84 1076 1138 2923
Fragments arranged by size:
2923 1138 1076 84
EarI CTCTTCn'nnn_
Cuts at: 839 1616 1805 1921 1993 3242 3452 839
Size: 777 189 116 72 1249 210 2608
Fragments arranged by size:
2608 1249 777 210 189 116 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 871 1572 1986 2110 3427 3739 3885 5031 871
Size: 701 414 124 1317 312 146 1146 1061
Fragments arranged by size:
1317 1146 1061 701 414 312 146 124
EcoICRI GAG'CTC
Cuts at: 149 1323 1455 2231 149
Size: 1174 132 776 3139
Fragments arranged by size:
3139 1174 776 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 184 346 436 604 700 844 1303 1350
Size: 21 162 90 168 96 144 459 47
Cuts at: 1350 3044 3476 4225 4493 163
Size: 1694 432 749 268 891
Fragments arranged by size:
1694 891 749 459 432 268 168 162
144 96 90 47 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2134 2134
Size: 5221
EcoO109I rG'GnC_Cy
Cuts at: 1632 2127 2358 1632
Size: 495 231 4495
Fragments arranged by size:
4495 495 231
EcoRI G'AATT_C
Cuts at: 346 2223 346
Size: 1877 3344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 5221
FseI GG_CCGG'CC
Cuts at: 470 470
Size: 5221
FspI TGC'GCA
Cuts at: 3000 3000
Size: 5221
HaeII r_GCGC'y
Cuts at: 791 911 1057 1215 2018 2653 2901 3925 4904
Size: 120 146 158 803 635 248 1024 979
Cuts at: 4904 791
Size: 1108
Fragments arranged by size:
1108 1024 979 803 635 248 158 146
120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 723 755 1342 1374 3061 3091 3093 3123 723
Size: 32 587 32 1687 30 2 30 2821
Fragments arranged by size:
2821 1687 587 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2258 2258
Size: 5221
KasI G'GCGC_C
Cuts at: 787 2649 2897 787
Size: 1862 248 3111
Fragments arranged by size:
3111 1862 248
KpnI G_GTAC'C
Cuts at: 1374 2239 1374
Size: 865 4356
MluI A'CGCG_T
Cuts at: 4654 4654
Size: 5221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 332 3892 4076 332
Size: 3560 184 1477
Fragments arranged by size:
3560 1477 184
MscI TGG'CCA
Cuts at: 1108 1201 1465 1540 2980 1108
Size: 93 264 75 1440 3349
Fragments arranged by size:
3349 1440 264 93 75
MslI CAynn'nnrTG
Cuts at: 274 721 871 1114 1162 1384 3208 3335 274
Size: 447 150 243 48 222 1824 127 2160
Fragments arranged by size:
2160 1824 447 243 222 150 127 48
MspA1I CmG'CkG
Cuts at: 818 1398 2171 2330 2644 3004 4019 4264 818
Size: 580 773 159 314 360 1015 245 1775
Fragments arranged by size:
1775 1015 773 580 360 314 245 159
NaeI GCC'GGC
Cuts at: 468 1017 1670 3400 468
Size: 549 653 1730 2289
Fragments arranged by size:
2289 1730 653 549
NarI GG'CG_CC
Cuts at: 788 2650 2898 788
Size: 1862 248 3111
Fragments arranged by size:
3111 1862 248
NcoI C'CATG_G
Cuts at: 125 806 125
Size: 681 4540
NgoMIV G'CCGG_C
Cuts at: 466 1015 1668 3398 466
Size: 549 653 1730 2289
Fragments arranged by size:
2289 1730 653 549
NruI TCG'CGA
Cuts at: 3582 3582
Size: 5221
NsiI A_TGCA'T
Cuts at: 241 241
Size: 5221
NspI r_CATG'y
Cuts at: 726 990 1119 1161 1410 1527 1767 1822 2272
Size: 264 129 42 249 117 240 55 450
Cuts at: 2272 2772 3681 726
Size: 500 909 2266
Fragments arranged by size:
2266 909 500 450 264 249 240 129
117 55 42
PciI A'CATG_T
Cuts at: 722 1523 3677 722
Size: 801 2154 2266
Fragments arranged by size:
2266 2154 801
PflMI CCAn_nnn'nTGG
Cuts at: 777 1295 1830 777
Size: 518 535 4168
Fragments arranged by size:
4168 535 518
PfoI T'CCnGG_A
Cuts at: 665 1547 665
Size: 882 4339
PmeI GTTT'AAAC
Cuts at: 2218 2218
Size: 5221
PmlI CAC'GTG
Cuts at: 2046 2046
Size: 5221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1213 1245 2131 2163 4360 4392 1213
Size: 32 886 32 2197 32 2042
Fragments arranged by size:
2197 2042 886 32 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 736 768 736
Size: 32 5189
PstI C_TGCA'G
Cuts at: 248 1211 1331 1943 2266 2951 248
Size: 963 120 612 323 685 2518
Fragments arranged by size:
2518 963 685 612 323 120
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5221
PvuII CAG'CTG
Cuts at: 2644 3004 2644
Size: 360 4861
RsrII CG'GwC_CG
Cuts at: 3414 3414
Size: 5221
SacI G_AGCT'C
Cuts at: 151 1325 1457 2233 151
Size: 1174 132 776 3139
Fragments arranged by size:
3139 1174 776 132
SalI G'TCGA_C
Cuts at: 2256 2256
Size: 5221
SapI GCTCTTCn'nnn_
Cuts at: 3242 3452 3242
Size: 210 5011
SbfI CC_TGCA'GG
Cuts at: 2266 2266
Size: 5221
ScaI AGT'ACT
Cuts at: 1087 1948 4738 1087
Size: 861 2790 1570
Fragments arranged by size:
2790 1570 861
SexAI A'CCwGG_T
Cuts at: 700 700
Size: 5221
SfcI C'TryA_G
Cuts at: 33 244 1207 1327 1939 2262 2947 3942 4133
Size: 211 963 120 612 323 685 995 191
Cuts at: 4133 33
Size: 1121
Fragments arranged by size:
1121 995 963 685 612 323 211 191
120
SfiI GGCCn_nnn'nGGCC
Cuts at: 463 463
Size: 5221
SfoI GGC'GCC
Cuts at: 789 2651 2899 789
Size: 1862 248 3111
Fragments arranged by size:
3111 1862 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5221
SmaI CCC'GGG
Cuts at: 441 2241 441
Size: 1800 3421
SmlI C'TyrA_G
Cuts at: 1216 1888 2374 3289 3783 4045 1216
Size: 672 486 915 494 262 2392
Fragments arranged by size:
2392 915 672 494 486 262
SnaBI TAC'GTA
Cuts at: 2211 2211
Size: 5221
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5221
SphI G_CATG'C
Cuts at: 1161 1822 2272 2772 1161
Size: 661 450 500 3610
Fragments arranged by size:
3610 661 500 450
SrfI GCCC'GGGC
Cuts at: 441 441
Size: 5221
StuI AGG'CCT
Cuts at: 1030 1030
Size: 5221
StyI C'CwwG_G
Cuts at: 125 635 806 911 1448 1466 1481 1628 1931
Size: 510 171 105 537 18 15 147 303
Cuts at: 1931 2153 2353 2707 125
Size: 222 200 354 2639
Fragments arranged by size:
2639 537 510 354 303 222 200 171
147 105 18 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1456 2802 2935 3394 3605 1456
Size: 1346 133 459 211 3072
Fragments arranged by size:
3072 1346 459 211 133
TatI w'GTAC_w
Cuts at: 623 1085 1112 1946 4736 623
Size: 462 27 834 2790 1108
Fragments arranged by size:
2790 1108 834 462 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4738 4770 4738
Size: 32 5189
Tth111I GACn'n_nGTC
Cuts at: 1645 3016 1645
Size: 1371 3850
XbaI T'CTAG_A
Cuts at: 2250 2250
Size: 5221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1311 1383 1635 1864 2714 1311
Size: 72 252 229 850 3818
Fragments arranged by size:
3818 850 252 229 72
XmaI C'CCGG_G
Cuts at: 439 2239 439
Size: 1800 3421
XmnI GAAnn'nnTTC
Cuts at: 208 829 208
Size: 621 4600
ZraI GAC'GTC
Cuts at: 975 975
Size: 5221
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI
BmgBI BmtI BsiWI BsmI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NdeI
NheI NotI PacI PasI PpuMI PshAI PsiI PspOMI
PspXI SacII SanDI SgrAI SspI SwaI XhoI