Restriction map for
pF1KSDA0781
(Circular) (Six-base) MAPSORT of: pF1KSDA0781.seq Check: 521 from: 1 to: 5917
pF1KSDA0781 5917 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2953 2953
Size: 5917
Acc65I G'GTAC_C
Cuts at: 2931 2931
Size: 5917
AclI AA'CG_TT
Cuts at: 5757 5757
Size: 5917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 1848 1915 2281 2506 3740 4172 4921 5189
Size: 1351 67 366 225 1234 432 749 268
Cuts at: 5189 497
Size: 1225
Fragments arranged by size:
1351 1234 1225 749 432 366 268 225
67
AfeI AGC'GCT
Cuts at: 2685 2685
Size: 5917
AflII C'TTAA_G
Cuts at: 1729 1729
Size: 5917
AflIII A'CryG_T
Cuts at: 3267 4373 5350 3267
Size: 1106 977 3834
Fragments arranged by size:
3834 1106 977
AgeI A'CCGG_T
Cuts at: 4305 4305
Size: 5917
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1235 1235
Size: 5917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 2258
Size: 32 5885
AlwNI CAG_nnn'CTG
Cuts at: 1026 1336 1452 1818 1938 2148 2163 2259 2376
Size: 310 116 366 120 210 15 96 117
Cuts at: 2376 2445 2529 2635 3220 4789 1026
Size: 69 84 106 585 1569 2154
Fragments arranged by size:
2154 1569 585 366 310 210 120 117
116 106 96 84 69 15
ApaLI G'TGCA_C
Cuts at: 541 4687 541
Size: 4146 1771
ApoI r'AATT_y
Cuts at: 434 490 618 1625 1955 2919 5270 5526 434
Size: 56 128 1007 330 964 2351 256 825
Fragments arranged by size:
2351 1007 964 825 330 256 128 56
AseI AT'TA_AT
Cuts at: 20 20
Size: 5917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5917
AvaI C'yCGr_G
Cuts at: 144 2549 2935 144
Size: 2405 386 3126
Fragments arranged by size:
3126 2405 386
BamHI G'GATC_C
Cuts at: 2871 2940 2871
Size: 69 5848
BanI G'GyrC_C
Cuts at: 239 2022 2931 3345 3593 3628 239
Size: 1783 909 414 248 35 2528
Fragments arranged by size:
2528 1783 909 414 248 35
BanII G_rGCy'C
Cuts at: 768 1244 1825 2353 2452 2742 2929 3959 768
Size: 476 581 528 99 290 187 1030 2726
Fragments arranged by size:
2726 1030 581 528 476 290 187 99
BbeI G_GCGC'C
Cuts at: 2026 3349 3597 2026
Size: 1323 248 4346
Fragments arranged by size:
4346 1323 248
BbsI GAAGACnn'nnnn_
Cuts at: 1523 1721 1523
Size: 198 5719
BbvCI CC'TCA_GC
Cuts at: 2036 2497 2036
Size: 461 5456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 135 169 135
Size: 34 5883
BciVI GTATCCnnnnn_n'
Cuts at: 2735 3813 4576 5917 2735
Size: 1078 763 1341 2735
Fragments arranged by size:
2735 1341 1078 763
BclI T'GATC_A
Cuts at: 3435 3435
Size: 5917
BfrBI ATG'CAT
Cuts at: 1257 1257
Size: 5917
BglI GCCn_nnn'nGGC
Cuts at: 1537 1813 1537
Size: 276 5641
BglII A'GATC_T
Cuts at: 3430 5469 3430
Size: 2039 3878
BlpI GC'TnA_GC
Cuts at: 1208 3027 1208
Size: 1819 4098
Bme1580I G_kGCm'C
Cuts at: 199 545 3540 3633 4691 199
Size: 346 2995 93 1058 1425
Fragments arranged by size:
2995 1425 1058 346 93
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1240 2151 3127 3208 3543 5689 1153
Size: 87 911 976 81 335 2146 1381
Fragments arranged by size:
2146 1381 976 911 335 87 81
BmtI G_CTAG'C
Cuts at: 2057 2057
Size: 5917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2484 2817 4282 2484
Size: 333 1465 4119
Fragments arranged by size:
4119 1465 333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 747 1513 1838 2026 2161 2170 2260 2479 2787
Size: 766 325 188 135 9 90 219 308
Cuts at: 2787 747
Size: 3877
Fragments arranged by size:
3877 766 325 308 219 188 135 90
9
Bpu10I CC'TnA_GC
Cuts at: 2036 2446 2497 2036
Size: 410 51 5456
Fragments arranged by size:
5456 410 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 2269 3091 3970 4464 4762 328
Size: 1941 822 879 494 298 1483
Fragments arranged by size:
1941 1483 879 822 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 379 954 1465 1785 1856 2146 5621 379
Size: 575 511 320 71 290 3475 675
Fragments arranged by size:
3475 675 575 511 320 290 71
BsaAI yAC'GTr
Cuts at: 2907 3268 2907
Size: 361 5556
BsaBI GATnn'nnATC
Cuts at: 6 3429 5468 6
Size: 3423 2039 455
Fragments arranged by size:
3423 2039 455
BsaHI Gr'CG_yC
Cuts at: 2023 3346 3594 4298 5794 2023
Size: 1323 248 704 1496 2146
Fragments arranged by size:
2146 1496 1323 704 248
BsaWI w'CCGG_w
Cuts at: 242 827 2001 2542 3104 3625 4305 4579 4726
Size: 585 1174 541 562 521 680 274 147
Cuts at: 4726 5137 242
Size: 411 1022
Fragments arranged by size:
1174 1022 680 585 562 541 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1633 1663 2143 2173 2258 2288 1633
Size: 30 480 30 85 30 5262
Fragments arranged by size:
5262 480 85 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1282 2024 2549 2552 1282
Size: 742 525 3 4647
Fragments arranged by size:
4647 742 525 3
BseYI C'CCAG_C
Cuts at: 201 231 1456 1803 2126 2185 2438 2640 2741
Size: 30 1225 347 323 59 253 202 101
Cuts at: 2741 3340 4098 4677 5173 201
Size: 599 758 579 496 945
Fragments arranged by size:
1225 945 758 599 579 496 347 323
253 202 101 59 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1996 1996
Size: 5917
BsiEI CG_ry'CG
Cuts at: 104 3457 3503 4713 104
Size: 3353 46 1210 1308
Fragments arranged by size:
3353 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 545 768 863 1825 2353 2929 3707 3897 4691
Size: 223 95 962 528 576 778 190 794
Cuts at: 4691 545
Size: 1771
Fragments arranged by size:
1771 962 794 778 576 528 223 190
95
BsmI GAATG_Cn'
Cuts at: 1257 1357 1257
Size: 100 5817
BsmBI CGTCTCn'nnnn_
Cuts at: 1560 2103 1560
Size: 543 5374
Bsp1286I G_dGCh'C
Cuts at: 199 545 768 863 1244 1825 2353 2452 2742
Size: 346 223 95 381 581 528 99 290
Cuts at: 2742 2929 3540 3633 3707 3897 3959 4691 199
Size: 187 611 93 74 190 62 732 1425
Fragments arranged by size:
1425 732 611 581 528 381 346 290
223 190 187 99 95 93 74 62
BspEI T'CCGG_A
Cuts at: 827 5137 827
Size: 4310 1607
BspHI T'CATG_A
Cuts at: 5093 5093
Size: 5917
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1011 2171 2965 3179 3481 3862 1011
Size: 1160 794 214 302 381 3066
Fragments arranged by size:
3066 1160 794 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1525 4207 4261 5747 5912 50
Size: 1475 2682 54 1486 165 55
Fragments arranged by size:
2682 1486 1475 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1202 3827 1202
Size: 2625 3292
BsrFI r'CCGG_y
Cuts at: 165 461 2025 3913 4094 4305 165
Size: 296 1564 1888 181 211 1777
Fragments arranged by size:
1888 1777 1564 296 211 181
BssSI C'ACGA_G
Cuts at: 4186 4546 4186
Size: 360 5557
BstAPI GCAn_nnn'nTGC
Cuts at: 1026 2259 3292 1026
Size: 1233 1033 3651
Fragments arranged by size:
3651 1233 1033
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5917
BstXI CCAn_nnnn'nTGG
Cuts at: 132 1599 132
Size: 1467 4450
BstYI r'GATC_y
Cuts at: 1 2871 2940 3407 3430 3765 4011 5025 5111
Size: 2870 69 467 23 335 246 1014 86
Cuts at: 5111 5123 5469 1
Size: 12 346 449
Fragments arranged by size:
2870 1014 467 449 346 335 246 86
69 23 12
Bsu36I CC'TnA_GG
Cuts at: 2066 2066
Size: 5917
BtgI C'CryG_G
Cuts at: 125 2137 2239 125
Size: 2012 102 3803
Fragments arranged by size:
3803 2012 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3865 4046 4209 3865
Size: 181 163 5573
Fragments arranged by size:
5573 181 163
BtsI GCAGTG_nn'
Cuts at: 305 1450 3158 305
Size: 1145 1708 3064
Fragments arranged by size:
3064 1708 1145
ClaI AT'CG_AT
Cuts at: 278 278
Size: 5917
DraI TTT'AAA
Cuts at: 627 2914 627
Size: 2287 3630
DrdI GACnn_nn'nnGTC
Cuts at: 3621 4481 3621
Size: 860 5057
EaeI y'GGCC_r
Cuts at: 459 1179 3500 3674 4065 4092 5439 459
Size: 720 2321 174 391 27 1347 937
Fragments arranged by size:
2321 1347 937 720 391 174 27
EagI C'GGCC_G
Cuts at: 3500 3500
Size: 5917
EarI CTCTTCn'nnn_
Cuts at: 794 1364 3938 4148 794
Size: 570 2574 210 2563
Fragments arranged by size:
2574 2563 570 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 1363 4123 4435 4581 5727 151
Size: 1212 2760 312 146 1146 341
Fragments arranged by size:
2760 1212 1146 341 312 146
EcoICRI GAG'CTC
Cuts at: 766 1823 2351 2927 766
Size: 1057 528 576 3756
Fragments arranged by size:
3756 1057 576 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 497 747 1513 1838 1848 1915 2026 2161 2170
Size: 250 766 325 10 67 111 135 9
Cuts at: 2170 2260 2281 2479 2506 2787 3740 4172 4921
Size: 90 21 198 27 281 953 432 749
Cuts at: 4921 5189 497
Size: 268 1225
Fragments arranged by size:
1225 953 766 749 432 325 281 268
250 198 135 111 90 67 27 21
10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1297 1616 1900 1297
Size: 319 284 5314
Fragments arranged by size:
5314 319 284
EcoO109I rG'GnC_Cy
Cuts at: 1698 2046 2418 2796 3054 1698
Size: 348 372 378 258 4561
Fragments arranged by size:
4561 378 372 348 258
EcoRI G'AATT_C
Cuts at: 1955 2919 1955
Size: 964 4953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 231 2307 2705 3934 231
Size: 2076 398 1229 2214
Fragments arranged by size:
2214 2076 1229 398
FspI TGC'GCA
Cuts at: 3696 3696
Size: 5917
HaeII r_GCGC'y
Cuts at: 1134 2026 2687 3349 3597 4621 5600 1134
Size: 892 661 662 248 1024 979 1451
Fragments arranged by size:
1451 1024 979 892 662 661 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 953 985 1274 1306 1633 1665 1669 1701 3757
Size: 32 289 32 327 32 4 32 2056
Cuts at: 3757 3787 3789 3819 953
Size: 30 2 30 3051
Fragments arranged by size:
3051 2056 327 289 32 32 32 32
30 30 4 2
HincII GTy'rAC
Cuts at: 2954 2954
Size: 5917
KasI G'GCGC_C
Cuts at: 2022 3345 3593 2022
Size: 1323 248 4346
Fragments arranged by size:
4346 1323 248
KpnI G_GTAC'C
Cuts at: 2935 2935
Size: 5917
MluI A'CGCG_T
Cuts at: 5350 5350
Size: 5917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 511 1331 4588 4772 511
Size: 820 3257 184 1656
Fragments arranged by size:
3257 1656 820 184
MscI TGG'CCA
Cuts at: 3676 3676
Size: 5917
MslI CAynn'nnrTG
Cuts at: 130 3904 4031 130
Size: 3774 127 2016
Fragments arranged by size:
3774 2016 127
MspA1I CmG'CkG
Cuts at: 291 717 1559 2060 2130 2241 2259 2376 3026
Size: 426 842 501 70 111 18 117 650
Cuts at: 3026 3340 3700 4715 4960 291
Size: 314 360 1015 245 1248
Fragments arranged by size:
1248 1015 842 650 501 426 360 314
245 117 111 70 18
NaeI GCC'GGC
Cuts at: 2027 4096 2027
Size: 2069 3848
NarI GG'CG_CC
Cuts at: 2023 3346 3594 2023
Size: 1323 248 4346
Fragments arranged by size:
4346 1323 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5917
NgoMIV G'CCGG_C
Cuts at: 2025 4094 2025
Size: 2069 3848
NheI G'CTAG_C
Cuts at: 2053 2053
Size: 5917
NruI TCG'CGA
Cuts at: 4278 4278
Size: 5917
NsiI A_TGCA'T
Cuts at: 1259 1259
Size: 5917
NspI r_CATG'y
Cuts at: 2968 3468 4377 2968
Size: 500 909 4508
Fragments arranged by size:
4508 909 500
PasI CC'CwG_GG
Cuts at: 1902 1902
Size: 5917
PciI A'CATG_T
Cuts at: 4373 4373
Size: 5917
PflMI CCAn_nnn'nTGG
Cuts at: 1336 1336
Size: 5917
PfoI T'CCnGG_A
Cuts at: 2042 2456 2042
Size: 414 5503
PmeI GTTT'AAAC
Cuts at: 2914 2914
Size: 5917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2258 2290 5056 5088 2258
Size: 32 2766 32 3087
Fragments arranged by size:
3087 2766 32 32
PpuMI rG'GwC_Cy
Cuts at: 1698 2046 1698
Size: 348 5569
PstI C_TGCA'G
Cuts at: 2168 2264 2318 2962 3647 2168
Size: 96 54 644 685 4438
Fragments arranged by size:
4438 685 644 96 54
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5917
PvuII CAG'CTG
Cuts at: 291 717 2060 2130 2259 2376 3340 3700 291
Size: 426 1343 70 129 117 964 360 2508
Fragments arranged by size:
2508 1343 964 426 360 129 117 70
RsrII CG'GwC_CG
Cuts at: 168 4110 168
Size: 3942 1975
SacI G_AGCT'C
Cuts at: 768 1825 2353 2929 768
Size: 1057 528 576 3756
Fragments arranged by size:
3756 1057 576 528
SacII CC_GC'GG
Cuts at: 2242 2242
Size: 5917
SalI G'TCGA_C
Cuts at: 2952 2952
Size: 5917
SapI GCTCTTCn'nnn_
Cuts at: 3938 4148 3938
Size: 210 5707
SbfI CC_TGCA'GG
Cuts at: 2962 2962
Size: 5917
ScaI AGT'ACT
Cuts at: 5434 5434
Size: 5917
SfcI C'TryA_G
Cuts at: 33 2164 2260 2314 2578 2958 3643 4638 4829
Size: 2131 96 54 264 380 685 995 191
Cuts at: 4829 33
Size: 1121
Fragments arranged by size:
2131 1121 995 685 380 264 191 96
54
SfoI GGC'GCC
Cuts at: 2024 3347 3595 2024
Size: 1323 248 4346
Fragments arranged by size:
4346 1323 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5917
SgrAI Cr'CCGG_yG
Cuts at: 2025 2025
Size: 5917
SmaI CCC'GGG
Cuts at: 2551 2937 2551
Size: 386 5531
SmlI C'TyrA_G
Cuts at: 307 1729 2248 3070 3985 4479 4741 307
Size: 1422 519 822 915 494 262 1483
Fragments arranged by size:
1483 1422 915 822 519 494 262
SnaBI TAC'GTA
Cuts at: 2907 2907
Size: 5917
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5917
SphI G_CATG'C
Cuts at: 2968 3468 2968
Size: 500 5417
StuI AGG'CCT
Cuts at: 1318 2320 1318
Size: 1002 4915
StyI C'CwwG_G
Cuts at: 125 1044 1223 3049 3403 125
Size: 919 179 1826 354 2639
Fragments arranged by size:
2639 1826 919 354 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 798 1915 3498 3631 4090 4301 798
Size: 1117 1583 133 459 211 2414
Fragments arranged by size:
2414 1583 1117 459 211 133
TatI w'GTAC_w
Cuts at: 327 5432 327
Size: 5105 812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1357 1389 2529 2561 5434 5466 1357
Size: 32 1140 32 2873 32 1808
Fragments arranged by size:
2873 1808 1140 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3712 3712
Size: 5917
XbaI T'CTAG_A
Cuts at: 1959 2946 1959
Size: 987 4930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 258 1464 3410 258
Size: 1206 1946 2765
Fragments arranged by size:
2765 1946 1206
XmaI C'CCGG_G
Cuts at: 2549 2935 2549
Size: 386 5531
XmnI GAAnn'nnTTC
Cuts at: 1627 1627
Size: 5917
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI ApaI AscI AvrII BaeI
BmgBI BsiWI BsrGI BssHII BstEII BstZ17I CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SrfI SspI SwaI XhoI ZraI