Restriction map for
pF1KSDA0760
(Circular) (Six-base) MAPSORT of: pF1KSDA0760.seq Check: 5771 from: 1 to: 6809
pF1KSDA0760 6809 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 157 2084 2232 2429 2798 3354 157
Size: 1927 148 197 369 556 3612
Fragments arranged by size:
3612 1927 556 369 197 148
AatII G_ACGT'C
Cuts at: 3197 3197
Size: 6809
AccI GT'mk_AC
Cuts at: 860 959 2011 3847 860
Size: 99 1052 1836 3822
Fragments arranged by size:
3822 1836 1052 99
Acc65I G'GTAC_C
Cuts at: 2478 3825 2478
Size: 1347 5462
AclI AA'CG_TT
Cuts at: 2744 6649 2744
Size: 3905 2904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 604 744 1252 1918 2298 4634 5066 5813 6081
Size: 140 508 666 380 2336 432 747 268
Cuts at: 6081 604
Size: 1332
Fragments arranged by size:
2336 1332 747 666 508 432 380 268
140
AfeI AGC'GCT
Cuts at: 2845 2845
Size: 6809
AflII C'TTAA_G
Cuts at: 2533 2533
Size: 6809
AflIII A'CryG_T
Cuts at: 1270 2585 2822 4161 5265 6242 1270
Size: 1315 237 1339 1104 977 1837
Fragments arranged by size:
1837 1339 1315 1104 977 237
AgeI A'CCGG_T
Cuts at: 5197 5197
Size: 6809
AhdI GACnn_n'nnGTC
Cuts at: 174 174
Size: 6809
AleI CACnn'nnGTG
Cuts at: 1568 2095 2323 1568
Size: 527 228 6054
Fragments arranged by size:
6054 527 228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 367 3707 367
Size: 3340 3469
AlwNI CAG_nnn'CTG
Cuts at: 1986 3081 3123 3180 4114 5681 1986
Size: 1095 42 57 934 1567 3114
Fragments arranged by size:
3114 1567 1095 934 57 42
ApaI G_GGCC'C
Cuts at: 207 2809 3404 207
Size: 2602 595 3612
Fragments arranged by size:
3612 2602 595
ApaLI G'TGCA_C
Cuts at: 447 615 2155 2245 3697 5579 447
Size: 168 1540 90 1452 1882 1677
Fragments arranged by size:
1882 1677 1540 1452 168 90
ApoI r'AATT_y
Cuts at: 3813 6162 6418 3813
Size: 2349 256 4204
Fragments arranged by size:
4204 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6809
AvaI C'yCGr_G
Cuts at: 1402 3274 3313 3829 1402
Size: 1872 39 516 4382
Fragments arranged by size:
4382 1872 516 39
BamHI G'GATC_C
Cuts at: 3834 3834
Size: 6809
BanI G'GyrC_C
Cuts at: 2478 2547 2908 3061 3412 3625 3825 4239 4487
Size: 69 361 153 351 213 200 414 248
Cuts at: 4487 4522 2478
Size: 35 4765
Fragments arranged by size:
4765 414 361 351 248 213 200 153
69 35
BanII G_rGCy'C
Cuts at: 207 1759 2597 2809 3050 3281 3404 3823 4853
Size: 1552 838 212 241 231 123 419 1030
Cuts at: 4853 207
Size: 2163
Fragments arranged by size:
2163 1552 1030 838 419 241 231 212
123
BbeI G_GCGC'C
Cuts at: 4243 4491 4243
Size: 248 6561
BbsI GAAGACnn'nnnn_
Cuts at: 592 846 1240 1775 2121 3350 3648 592
Size: 254 394 535 346 1229 298 3753
Fragments arranged by size:
3753 1229 535 394 346 298 254
BciVI GTATCCnnnnn_n'
Cuts at: 1850 4707 5468 6809 1850
Size: 2857 761 1341 1850
Fragments arranged by size:
2857 1850 1341 761
BclI T'GATC_A
Cuts at: 4329 4329
Size: 6809
BglI GCCn_nnn'nGGC
Cuts at: 2267 2267
Size: 6809
BglII A'GATC_T
Cuts at: 1277 3716 4324 6361 1277
Size: 2439 608 2037 1725
Fragments arranged by size:
2439 2037 1725 608
BlpI GC'TnA_GC
Cuts at: 2504 3093 3921 2504
Size: 589 828 5392
Fragments arranged by size:
5392 828 589
Bme1580I G_kGCm'C
Cuts at: 207 346 451 619 922 931 1939 2159 2249
Size: 139 105 168 303 9 1008 220 90
Cuts at: 2249 2809 2911 3404 3434 3701 3739 4434 4527
Size: 560 102 493 30 267 38 695 93
Cuts at: 4527 5583 207
Size: 1056 1433
Fragments arranged by size:
1433 1056 1008 695 560 493 303 267
220 168 139 105 102 93 90 38
30 9
BmgBI CAC'GTC
Cuts at: 2009 2250 2580 2009
Size: 241 330 6238
Fragments arranged by size:
6238 330 241
BmrI ACTGGGnnnn_n'
Cuts at: 1036 1753 1935 2223 4021 4102 4437 6581 1036
Size: 717 182 288 1798 81 335 2144 1264
Fragments arranged by size:
2144 1798 1264 717 335 288 182 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 957 1104 5176 957
Size: 147 4072 2590
Fragments arranged by size:
4072 2590 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 252 582 799 978 1050 1240 1930 2431
Size: 13 330 217 179 72 190 690 501
Cuts at: 2431 3370 239
Size: 939 3678
Fragments arranged by size:
3678 939 690 501 330 217 190 179
72 13
Bpu10I CC'TnA_GC
Cuts at: 932 932
Size: 6809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 556 1846 3034 3064 3139 3985 4864 5356
Size: 142 1290 1188 30 75 846 879 492
Cuts at: 5356 5654 414
Size: 298 1569
Fragments arranged by size:
1569 1290 1188 879 846 492 298 142
75 30
BsaI GGTCTCn'nnnn_
Cuts at: 2302 3385 6513 2302
Size: 1083 3128 2598
Fragments arranged by size:
3128 2598 1083
BsaAI yAC'GTr
Cuts at: 789 3801 4162 789
Size: 3012 361 3436
Fragments arranged by size:
3436 3012 361
BsaBI GATnn'nnATC
Cuts at: 6 3496 4323 6360 6
Size: 3490 827 2037 455
Fragments arranged by size:
3490 2037 827 455
BsaHI Gr'CG_yC
Cuts at: 3100 3194 4240 4488 5190 6686 3100
Size: 94 1046 248 702 1496 3223
Fragments arranged by size:
3223 1496 1046 702 248 94
BsaWI w'CCGG_w
Cuts at: 1596 3998 4519 5197 5471 5618 6029 1596
Size: 2402 521 678 274 147 411 2376
Fragments arranged by size:
2402 2376 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1495 1525 1810 1840 1945 1975 2320 2350 2584
Size: 30 285 30 105 30 345 30 234
Cuts at: 2584 2614 1495
Size: 30 5690
Fragments arranged by size:
5690 345 285 234 105 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 495 630 787 933 2959 2962 3574 346
Size: 149 135 157 146 2026 3 612 3581
Fragments arranged by size:
3581 2026 612 157 149 146 135 3
BseYI C'CCAG_C
Cuts at: 1256 4234 4992 5569 6065 1256
Size: 2978 758 577 496 2000
Fragments arranged by size:
2978 2000 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1624 1632 1888 2094 2184 3339 1318
Size: 306 8 256 206 90 1155 4788
Fragments arranged by size:
4788 1155 306 256 206 90 8
BsiEI CG_ry'CG
Cuts at: 104 492 2456 2683 3288 4351 4397 5605 104
Size: 388 1964 227 605 1063 46 1208 1308
Fragments arranged by size:
1964 1308 1208 1063 605 388 227 46
BsiHKAI G_wGCw'C
Cuts at: 451 619 794 1322 1439 1513 1550 2159 2249
Size: 168 175 528 117 74 37 609 90
Cuts at: 2249 2789 2963 3050 3605 3701 3823 4601 4791
Size: 540 174 87 555 96 122 778 190
Cuts at: 4791 5583 451
Size: 792 1677
Fragments arranged by size:
1677 792 778 609 555 540 528 190
175 174 168 122 117 96 90 87
74 37
BsmI GAATG_Cn'
Cuts at: 836 836
Size: 6809
BsmBI CGTCTCn'nnnn_
Cuts at: 266 1452 266
Size: 1186 5623
Bsp1286I G_dGCh'C
Cuts at: 207 346 451 619 794 922 931 1322 1439
Size: 139 105 168 175 128 9 391 117
Cuts at: 1439 1513 1550 1759 1939 2159 2249 2597 2789
Size: 74 37 209 180 220 90 348 192
Cuts at: 2789 2809 2911 2963 3050 3281 3404 3434 3605
Size: 20 102 52 87 231 123 30 171
Cuts at: 3605 3701 3739 3823 4434 4527 4601 4791 4853
Size: 96 38 84 611 93 74 190 62
Cuts at: 4853 5583 207
Size: 730 1433
Fragments arranged by size:
1433 730 611 391 348 231 220 209
192 190 180 175 171 168 139 128
123 117 105 102 96 93 90 87
84 74 74 62 52 38 37 30
20 9
BspEI T'CCGG_A
Cuts at: 6029 6029
Size: 6809
BspHI T'CATG_A
Cuts at: 5985 5985
Size: 6809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 836 2072 2084 2232 2246 2429 2525 2798
Size: 679 1236 12 148 14 183 96 273
Cuts at: 2798 2990 3354 3859 4073 4375 4756 157
Size: 192 364 505 214 302 381 2210
Fragments arranged by size:
2210 1236 679 505 381 364 302 273
214 192 183 148 96 14 12
BsrBI CCG'CTC
Cuts at: 50 5101 5155 6639 6804 50
Size: 5051 54 1484 165 55
Fragments arranged by size:
5051 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2184 4721 2184
Size: 2537 4272
BsrFI r'CCGG_y
Cuts at: 491 980 2657 3223 3287 3298 4807 4988 5197
Size: 489 1677 566 64 11 1509 181 209
Cuts at: 5197 491
Size: 2103
Fragments arranged by size:
2103 1677 1509 566 489 209 181 64
11
BssSI C'ACGA_G
Cuts at: 412 535 3538 5080 5438 412
Size: 123 3003 1542 358 1783
Fragments arranged by size:
3003 1783 1542 358 123
BstAPI GCAn_nnn'nTGC
Cuts at: 455 623 842 1397 1508 1853 2207 2526 3461
Size: 168 219 555 111 345 354 319 935
Cuts at: 3461 4186 455
Size: 725 3078
Fragments arranged by size:
3078 935 725 555 354 345 319 219
168 111
BstBI TT'CG_AA
Cuts at: 110 5170 110
Size: 5060 1749
BstEII G'GTnAC_C
Cuts at: 2880 2880
Size: 6809
BstYI r'GATC_y
Cuts at: 1 419 575 1223 1277 3053 3491 3716 3834
Size: 418 156 648 54 1776 438 225 118
Cuts at: 3834 4301 4324 4659 4905 5917 6003 6015 6361
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6361 1
Size: 449
Fragments arranged by size:
1776 1012 648 467 449 438 418 346
335 246 225 156 118 86 54 23
12
Bsu36I CC'TnA_GG
Cuts at: 848 2543 848
Size: 1695 5114
BtgI C'CryG_G
Cuts at: 125 1514 2633 3057 125
Size: 1389 1119 424 3877
Fragments arranged by size:
3877 1389 1119 424
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3407 4759 4940 5103 3407
Size: 1352 181 163 5113
Fragments arranged by size:
5113 1352 181 163
BtsI GCAGTG_nn'
Cuts at: 528 838 840 871 1386 1452 1563 1575 1584
Size: 310 2 31 515 66 111 12 9
Cuts at: 1584 2106 2341 2961 3327 4052 528
Size: 522 235 620 366 725 3285
Fragments arranged by size:
3285 725 620 522 515 366 310 235
111 66 31 12 9 2
DraI TTT'AAA
Cuts at: 3808 3808
Size: 6809
DraIII CAC_nnn'GTG
Cuts at: 623 2022 2117 2439 2880 623
Size: 1399 95 322 441 4552
Fragments arranged by size:
4552 1399 441 322 95
DrdI GACnn_nn'nnGTC
Cuts at: 4515 5373 4515
Size: 858 5951
EaeI y'GGCC_r
Cuts at: 698 1211 2276 2468 2489 2680 3114 3575 4394
Size: 513 1065 192 21 191 434 461 819
Cuts at: 4394 4568 4959 4986 6331 698
Size: 174 391 27 1345 1176
Fragments arranged by size:
1345 1176 1065 819 513 461 434 391
192 191 174 27 21
EagI C'GGCC_G
Cuts at: 2680 4394 2680
Size: 1714 5095
EarI CTCTTCn'nnn_
Cuts at: 400 472 4832 5042 400
Size: 72 4360 210 2167
Fragments arranged by size:
4360 2167 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 838 5017 5327 5473 6619 838
Size: 4179 310 146 1146 1028
Fragments arranged by size:
4179 1146 1028 310 146
EcoICRI GAG'CTC
Cuts at: 3048 3821 3048
Size: 773 6036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 252 582 604 744 799 978 1050 1240
Size: 13 330 22 140 55 179 72 190
Cuts at: 1240 1252 1918 1930 2298 2431 3370 4634 5066
Size: 12 666 12 368 133 939 1264 432
Cuts at: 5066 5813 6081 239
Size: 747 268 967
Fragments arranged by size:
1264 967 939 747 666 432 368 330
268 190 179 140 133 72 55 22
13 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 328 2119 328
Size: 1791 5018
EcoO109I rG'GnC_Cy
Cuts at: 2805 2806 3177 3401 3948 2805
Size: 1 371 224 547 5666
Fragments arranged by size:
5666 547 371 224 1
EcoRI G'AATT_C
Cuts at: 3813 3813
Size: 6809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 651 747 1215 2313 3768 4828 651
Size: 96 468 1098 1455 1060 2632
Fragments arranged by size:
2632 1455 1098 1060 468 96
FspI TGC'GCA
Cuts at: 2986 4590 2986
Size: 1604 5205
HaeII r_GCGC'y
Cuts at: 1055 1073 1817 2847 3245 4243 4491 5513 6492
Size: 18 744 1030 398 998 248 1022 979
Cuts at: 6492 1055
Size: 1372
Fragments arranged by size:
1372 1030 1022 998 979 744 398 248
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 121 153 160 192 268 300 1945 1977 2683
Size: 32 7 32 76 32 1645 32 706
Cuts at: 2683 2715 4651 4681 4683 4713 121
Size: 32 1936 30 2 30 2217
Fragments arranged by size:
2217 1936 1645 706 76 32 32 32
32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 861 2012 3848 861
Size: 1151 1836 3822
Fragments arranged by size:
3822 1836 1151
HindIII A'AGCT_T
Cuts at: 3187 3187
Size: 6809
KasI G'GCGC_C
Cuts at: 4239 4487 4239
Size: 248 6561
KpnI G_GTAC'C
Cuts at: 2482 3829 2482
Size: 1347 5462
MluI A'CGCG_T
Cuts at: 2585 6242 2585
Size: 3657 3152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 686 1895 2570 5480 5664 686
Size: 1209 675 2910 184 1831
Fragments arranged by size:
2910 1831 1209 675 184
MscI TGG'CCA
Cuts at: 700 2491 3116 4570 700
Size: 1791 625 1454 2939
Fragments arranged by size:
2939 1791 1454 625
MslI CAynn'nnrTG
Cuts at: 295 1568 2095 2152 2177 2323 2638 3364 3450
Size: 1273 527 57 25 146 315 726 86
Cuts at: 3450 4798 4925 295
Size: 1348 127 2179
Fragments arranged by size:
2179 1348 1273 726 527 315 146 127
86 57 25
MspA1I CmG'CkG
Cuts at: 212 810 1116 1800 2198 3008 3097 3920 4234
Size: 598 306 684 398 810 89 823 314
Cuts at: 4234 4594 5607 5852 212
Size: 360 1013 245 1169
Fragments arranged by size:
1169 1013 823 810 684 598 398 360
314 306 245 89
NaeI GCC'GGC
Cuts at: 3225 3300 4990 3225
Size: 75 1690 5044
Fragments arranged by size:
5044 1690 75
NarI GG'CG_CC
Cuts at: 4240 4488 4240
Size: 248 6561
NcoI C'CATG_G
Cuts at: 125 1514 2633 3057 125
Size: 1389 1119 424 3877
Fragments arranged by size:
3877 1389 1119 424
NgoMIV G'CCGG_C
Cuts at: 3223 3298 4988 3223
Size: 75 1690 5044
Fragments arranged by size:
5044 1690 75
NspI r_CATG'y
Cuts at: 669 1274 2088 2529 2826 3024 3075 3363 3862
Size: 605 814 441 297 198 51 288 499
Cuts at: 3862 4362 5269 669
Size: 500 907 2209
Fragments arranged by size:
2209 907 814 605 500 499 441 297
288 198 51
PciI A'CATG_T
Cuts at: 1270 2822 5265 1270
Size: 1552 2443 2814
Fragments arranged by size:
2814 2443 1552
PfoI T'CCnGG_A
Cuts at: 216 216
Size: 6809
PmeI GTTT'AAAC
Cuts at: 3808 3808
Size: 6809
PmlI CAC'GTG
Cuts at: 789 789
Size: 6809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5948 5980 5948
Size: 32 6777
PpuMI rG'GwC_Cy
Cuts at: 3177 3177
Size: 6809
PspOMI G'GGCC_C
Cuts at: 203 2805 3400 203
Size: 2602 595 3612
Fragments arranged by size:
3612 2602 595
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1333 1365 1333
Size: 32 6777
PstI C_TGCA'G
Cuts at: 460 659 833 1433 1570 1982 2069 2243 2336
Size: 199 174 600 137 412 87 174 93
Cuts at: 2336 2383 2522 2651 2795 2939 3128 3770 3856
Size: 47 139 129 144 144 189 642 86
Cuts at: 3856 4541 460
Size: 685 2728
Fragments arranged by size:
2728 685 642 600 412 199 189 174
174 144 144 139 137 129 93 87
86 47
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6809
PvuII CAG'CTG
Cuts at: 810 4234 4594 810
Size: 3424 360 3025
Fragments arranged by size:
3424 3025 360
RsrII CG'GwC_CG
Cuts at: 5004 5004
Size: 6809
SacI G_AGCT'C
Cuts at: 3050 3823 3050
Size: 773 6036
SalI G'TCGA_C
Cuts at: 859 2010 3846 859
Size: 1151 1836 3822
Fragments arranged by size:
3822 1836 1151
SapI GCTCTTCn'nnn_
Cuts at: 4832 5042 4832
Size: 210 6599
SbfI CC_TGCA'GG
Cuts at: 1433 2243 2522 3856 1433
Size: 810 279 1334 4386
Fragments arranged by size:
4386 1334 810 279
ScaI AGT'ACT
Cuts at: 6326 6326
Size: 6809
SexAI A'CCwGG_T
Cuts at: 3179 3179
Size: 6809
SfcI C'TryA_G
Cuts at: 33 456 655 829 1170 1429 1566 1978 2065
Size: 423 199 174 341 259 137 412 87
Cuts at: 2065 2239 2332 2379 2518 2647 2791 2935 3124
Size: 174 93 47 139 129 144 144 189
Cuts at: 3124 3766 3852 4537 5530 5721 33
Size: 642 86 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 423 412 341 259
199 191 189 174 174 144 144 139
137 129 93 87 86 47
SfoI GGC'GCC
Cuts at: 4241 4489 4241
Size: 248 6561
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6809
SmaI CCC'GGG
Cuts at: 3831 3831
Size: 6809
SmlI C'TyrA_G
Cuts at: 393 571 1861 2533 3043 3049 3154 3964 4879
Size: 178 1290 672 510 6 105 810 915
Cuts at: 4879 5371 5633 393
Size: 492 262 1569
Fragments arranged by size:
1569 1290 915 810 672 510 492 262
178 105 6
SnaBI TAC'GTA
Cuts at: 3801 3801
Size: 6809
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6809
SphI G_CATG'C
Cuts at: 2529 3024 3862 4362 2529
Size: 495 838 500 4976
Fragments arranged by size:
4976 838 500 495
StuI AGG'CCT
Cuts at: 2389 2389
Size: 6809
StyI C'CwwG_G
Cuts at: 125 1160 1514 1742 2138 2279 2633 3057 3943
Size: 1035 354 228 396 141 354 424 886
Cuts at: 3943 4297 125
Size: 354 2637
Fragments arranged by size:
2637 1035 886 424 396 354 354 354
228 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 240 303 2470 3247 3430 3775 4392 4525 4984
Size: 63 2167 777 183 345 617 133 459
Cuts at: 4984 5193 240
Size: 209 1856
Fragments arranged by size:
2167 1856 777 617 459 345 209 183
133 63
TatI w'GTAC_w
Cuts at: 1023 6324 1023
Size: 5301 1508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 2320 2352 6326 6358 817
Size: 32 1471 32 3974 32 1268
Fragments arranged by size:
3974 1471 1268 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2007 4606 2007
Size: 2599 4210
XbaI T'CTAG_A
Cuts at: 3840 3840
Size: 6809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 214 245 1074 2406 3252 4304 214
Size: 31 829 1332 846 1052 2719
Fragments arranged by size:
2719 1332 1052 846 829 31
XmaI C'CCGG_G
Cuts at: 3829 3829
Size: 6809
XmnI GAAnn'nnTTC
Cuts at: 3748 3748
Size: 6809
ZraI GAC'GTC
Cuts at: 3195 3195
Size: 6809
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
BsiWI BsrGI BssHII BstXI BstZ17I ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PshAI PsiI PspXI SacII
SanDI SfiI SgrAI SrfI SspI SwaI XhoI