Restriction map for
pF1KSDA0758
(Circular) (Six-base) MAPSORT of: pF1KSDA0758.seq Check: 3744 from: 1 to: 7175
pF1KSDA0758 7175 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1150 3289 1150
Size: 2139 5036
AatII G_ACGT'C
Cuts at: 557 1805 557
Size: 1248 5927
AccI GT'mk_AC
Cuts at: 211 4213 211
Size: 4002 3173
Acc65I G'GTAC_C
Cuts at: 4191 4191
Size: 7175
AclI AA'CG_TT
Cuts at: 2198 7015 2198
Size: 4817 2358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 607 2392 2951 2985 3958 5000 5432 6179
Size: 281 1785 559 34 973 1042 432 747
Cuts at: 6179 6447 326
Size: 268 1054
Fragments arranged by size:
1785 1054 1042 973 747 559 432 281
268 34
AflIII A'CryG_T
Cuts at: 4527 5631 6608 4527
Size: 1104 977 5094
Fragments arranged by size:
5094 1104 977
AgeI A'CCGG_T
Cuts at: 5563 5563
Size: 7175
AleI CACnn'nnGTG
Cuts at: 3697 3838 4027 3697
Size: 141 189 6845
Fragments arranged by size:
6845 189 141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 679 711 721 753 679
Size: 32 10 32 7101
Fragments arranged by size:
7101 32 32 10
AlwNI CAG_nnn'CTG
Cuts at: 4480 6047 4480
Size: 1567 5608
ApaLI G'TGCA_C
Cuts at: 5945 5945
Size: 7175
ApoI r'AATT_y
Cuts at: 755 1238 1776 2487 4179 6528 6784 755
Size: 483 538 711 1692 2349 256 1146
Fragments arranged by size:
2349 1692 1146 711 538 483 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7175
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7175
AvaI C'yCGr_G
Cuts at: 512 3581 4195 512
Size: 3069 614 3492
Fragments arranged by size:
3492 3069 614
BamHI G'GATC_C
Cuts at: 357 2136 4200 357
Size: 1779 2064 3332
Fragments arranged by size:
3332 2064 1779
BanI G'GyrC_C
Cuts at: 569 4191 4605 4853 4888 569
Size: 3622 414 248 35 2856
Fragments arranged by size:
3622 2856 414 248 35
BanII G_rGCy'C
Cuts at: 2089 2341 4189 5219 2089
Size: 252 1848 1030 4045
Fragments arranged by size:
4045 1848 1030 252
BbeI G_GCGC'C
Cuts at: 4609 4857 4609
Size: 248 6927
BbsI GAAGACnn'nnnn_
Cuts at: 223 517 549 683 2982 3417 3885 3977 223
Size: 294 32 134 2299 435 468 92 3421
Fragments arranged by size:
3421 2299 468 435 294 134 92 32
BbvCI CC'TCA_GC
Cuts at: 3418 3418
Size: 7175
BciVI GTATCCnnnnn_n'
Cuts at: 5073 5834 7175 5073
Size: 761 1341 5073
Fragments arranged by size:
5073 1341 761
BclI T'GATC_A
Cuts at: 2330 3665 4695 2330
Size: 1335 1030 4810
Fragments arranged by size:
4810 1335 1030
BfrBI ATG'CAT
Cuts at: 346 1601 346
Size: 1255 5920
BglI GCCn_nnn'nGGC
Cuts at: 2969 3385 3643 2969
Size: 416 258 6501
Fragments arranged by size:
6501 416 258
BglII A'GATC_T
Cuts at: 4690 6727 4690
Size: 2037 5138
BlpI GC'TnA_GC
Cuts at: 4287 4287
Size: 7175
Bme1580I G_kGCm'C
Cuts at: 572 1435 4800 4893 5949 572
Size: 863 3365 93 1056 1798
Fragments arranged by size:
3365 1798 1056 863 93
BmrI ACTGGGnnnn_n'
Cuts at: 1422 2253 3454 3630 4387 4468 4803 6947 1422
Size: 831 1201 176 757 81 335 2144 1650
Fragments arranged by size:
2144 1650 1201 831 757 335 176 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2360 5542 2360
Size: 3182 3993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 2296 1133
Size: 1163 6012
Bpu10I CC'TnA_GC
Cuts at: 2321 2855 3418 2321
Size: 534 563 6078
Fragments arranged by size:
6078 563 534
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1396 3219 4351 5230 5722 6020 868
Size: 528 1823 1132 879 492 298 2023
Fragments arranged by size:
2023 1823 1132 879 528 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3835 6879 3835
Size: 3044 4131
BsaAI yAC'GTr
Cuts at: 2508 4167 4528 2508
Size: 1659 361 5155
Fragments arranged by size:
5155 1659 361
BsaBI GATnn'nnATC
Cuts at: 6 4689 6726 6
Size: 4683 2037 455
Fragments arranged by size:
4683 2037 455
BsaHI Gr'CG_yC
Cuts at: 554 1802 4606 4854 5556 7052 554
Size: 1248 2804 248 702 1496 677
Fragments arranged by size:
2804 1496 1248 702 677 248
BsaWI w'CCGG_w
Cuts at: 740 4364 4885 5563 5837 5984 6395 740
Size: 3624 521 678 274 147 411 1520
Fragments arranged by size:
3624 1520 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 679 709 901 931 679
Size: 30 192 30 6923
Fragments arranged by size:
6923 192 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1698 1918 2281 1698
Size: 220 363 6592
Fragments arranged by size:
6592 363 220
BseYI C'CCAG_C
Cuts at: 2203 3564 3575 3657 4600 5358 5935 6431 2203
Size: 1361 11 82 943 758 577 496 2947
Fragments arranged by size:
2947 1361 943 758 577 496 82 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 173 2698 173
Size: 2525 4650
BsiEI CG_ry'CG
Cuts at: 104 4717 4763 5971 104
Size: 4613 46 1208 1308
Fragments arranged by size:
4613 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 487 2513 4189 4967 5157 5949 487
Size: 2026 1676 778 190 792 1713
Fragments arranged by size:
2026 1713 1676 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 487 572 1435 2089 2341 2513 4189 4800 4893
Size: 85 863 654 252 172 1676 611 93
Cuts at: 4893 4967 5157 5219 5949 487
Size: 74 190 62 730 1713
Fragments arranged by size:
1713 1676 863 730 654 611 252 190
172 93 85 74 62
BspEI T'CCGG_A
Cuts at: 740 6395 740
Size: 5655 1520
BspHI T'CATG_A
Cuts at: 234 683 6351 234
Size: 449 5668 1058
Fragments arranged by size:
5668 1058 449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 471 1150 3289 3485 4225 4439 4741 5122 471
Size: 679 2139 196 740 214 302 381 2524
Fragments arranged by size:
2524 2139 740 679 381 302 214 196
BsrBI CCG'CTC
Cuts at: 50 5467 5521 7005 7170 50
Size: 5417 54 1484 165 55
Fragments arranged by size:
5417 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 573 2581 2769 3512 5087 573
Size: 2008 188 743 1575 2661
Fragments arranged by size:
2661 2008 1575 743 188
BsrFI r'CCGG_y
Cuts at: 2186 5173 5354 5563 2186
Size: 2987 181 209 3798
Fragments arranged by size:
3798 2987 209 181
BsrGI T'GTAC_A
Cuts at: 879 879
Size: 7175
BssSI C'ACGA_G
Cuts at: 1680 5446 5804 1680
Size: 3766 358 3051
Fragments arranged by size:
3766 3051 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3041 4552 3041
Size: 1511 5664
BstBI TT'CG_AA
Cuts at: 110 5536 110
Size: 5426 1749
BstEII G'GTnAC_C
Cuts at: 3246 3246
Size: 7175
BstYI r'GATC_y
Cuts at: 1 357 2136 2376 3132 3713 3933 4200 4667
Size: 356 1779 240 756 581 220 267 467
Cuts at: 4667 4690 5025 5271 6283 6369 6381 6727 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1779 1012 756 581 467 449 356 346
335 267 246 240 220 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3718 3718
Size: 7175
BtgI C'CryG_G
Cuts at: 2822 2822
Size: 7175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5125 5306 5469 5125
Size: 181 163 6831
Fragments arranged by size:
6831 181 163
BtsI GCAGTG_nn'
Cuts at: 1509 1755 1878 3042 4418 1509
Size: 246 123 1164 1376 4266
Fragments arranged by size:
4266 1376 1164 246 123
DraI TTT'AAA
Cuts at: 4174 4174
Size: 7175
DraIII CAC_nnn'GTG
Cuts at: 152 152
Size: 7175
DrdI GACnn_nn'nnGTC
Cuts at: 1119 4881 5739 1119
Size: 3762 858 2555
Fragments arranged by size:
3762 2555 858
EaeI y'GGCC_r
Cuts at: 1535 4760 4934 5325 5352 6697 1535
Size: 3225 174 391 27 1345 2013
Fragments arranged by size:
3225 2013 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4760 4760
Size: 7175
EarI CTCTTCn'nnn_
Cuts at: 251 2266 2424 3751 5198 5408 251
Size: 2015 158 1327 1447 210 2018
Fragments arranged by size:
2018 2015 1447 1327 210 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 687 5383 5693 5839 6985 687
Size: 4696 310 146 1146 877
Fragments arranged by size:
4696 1146 877 310 146
EcoICRI GAG'CTC
Cuts at: 4187 4187
Size: 7175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 607 1133 2296 2392 2951 2985 3958 5000
Size: 281 526 1163 96 559 34 973 1042
Cuts at: 5000 5432 6179 6447 326
Size: 432 747 268 1054
Fragments arranged by size:
1163 1054 1042 973 747 559 526 432
281 268 96 34
EcoNI CCTnn'n_nnAGG
Cuts at: 622 1731 2854 622
Size: 1109 1123 4943
Fragments arranged by size:
4943 1123 1109
EcoO109I rG'GnC_Cy
Cuts at: 710 3636 3792 4314 710
Size: 2926 156 522 3571
Fragments arranged by size:
3571 2926 522 156
EcoRI G'AATT_C
Cuts at: 1776 4179 1776
Size: 2403 4772
EcoRV GAT'ATC
Cuts at: 2862 2862
Size: 7175
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 741 2562 2866 3006 5194 741
Size: 1821 304 140 2188 2722
Fragments arranged by size:
2722 2188 1821 304 140
FspI TGC'GCA
Cuts at: 4956 4956
Size: 7175
HaeII r_GCGC'y
Cuts at: 1041 4609 4857 5879 6858 1041
Size: 3568 248 1022 979 1358
Fragments arranged by size:
3568 1358 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 876 901 908 933 1394 1426 1674 1706 2272
Size: 25 7 25 461 32 248 32 566
Cuts at: 2272 2304 2449 2481 3240 3272 5017 5047 5049
Size: 32 145 32 759 32 1745 30 2
Cuts at: 5049 5079 876
Size: 30 2972
Fragments arranged by size:
2972 1745 759 566 461 248 145 32
32 32 32 32 30 30 25 25
7 2
HincII GTy'rAC
Cuts at: 1971 4004 4214 1971
Size: 2033 210 4932
Fragments arranged by size:
4932 2033 210
HindIII A'AGCT_T
Cuts at: 3950 3950
Size: 7175
HpaI GTT'AAC
Cuts at: 1971 1971
Size: 7175
KasI G'GCGC_C
Cuts at: 4605 4853 4605
Size: 248 6927
KpnI G_GTAC'C
Cuts at: 4195 4195
Size: 7175
MluI A'CGCG_T
Cuts at: 6608 6608
Size: 7175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1037 2133 2500 2783 5846 6030 1037
Size: 1096 367 283 3063 184 2182
Fragments arranged by size:
3063 2182 1096 367 283 184
MscI TGG'CCA
Cuts at: 1537 4936 1537
Size: 3399 3776
MslI CAynn'nnrTG
Cuts at: 239 442 1516 1531 3112 3673 3697 3838 4027
Size: 203 1074 15 1581 561 24 141 189
Cuts at: 4027 5164 5291 239
Size: 1137 127 2123
Fragments arranged by size:
2123 1581 1137 1074 561 203 189 141
127 24 15
MspA1I CmG'CkG
Cuts at: 250 1818 4286 4600 4960 5973 6218 250
Size: 1568 2468 314 360 1013 245 1207
Fragments arranged by size:
2468 1568 1207 1013 360 314 245
NaeI GCC'GGC
Cuts at: 5356 5356
Size: 7175
NarI GG'CG_CC
Cuts at: 4606 4854 4606
Size: 248 6927
NcoI C'CATG_G
Cuts at: 2822 2822
Size: 7175
NdeI CA'TA_TG
Cuts at: 819 1934 819
Size: 1115 6060
NgoMIV G'CCGG_C
Cuts at: 5354 5354
Size: 7175
NsiI A_TGCA'T
Cuts at: 348 1603 348
Size: 1255 5920
NspI r_CATG'y
Cuts at: 2132 4228 4728 5635 2132
Size: 2096 500 907 3672
Fragments arranged by size:
3672 2096 907 500
PasI CC'CwG_GG
Cuts at: 565 3847 4011 565
Size: 3282 164 3729
Fragments arranged by size:
3729 3282 164
PciI A'CATG_T
Cuts at: 5631 5631
Size: 7175
PflMI CCAn_nnn'nTGG
Cuts at: 400 1242 1739 3323 400
Size: 842 497 1584 4252
Fragments arranged by size:
4252 1584 842 497
PfoI T'CCnGG_A
Cuts at: 353 1362 2864 3158 3347 353
Size: 1009 1502 294 189 4181
Fragments arranged by size:
4181 1502 1009 294 189
PmeI GTTT'AAAC
Cuts at: 4174 4174
Size: 7175
PmlI CAC'GTG
Cuts at: 2508 2508
Size: 7175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 679 711 6314 6346 679
Size: 32 5603 32 1508
Fragments arranged by size:
5603 1508 32 32
PpuMI rG'GwC_Cy
Cuts at: 710 3792 710
Size: 3082 4093
PshAI GACnn'nnGTC
Cuts at: 451 451
Size: 7175
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 187 219 863 895 187
Size: 32 644 32 6467
Fragments arranged by size:
6467 644 32 32
PstI C_TGCA'G
Cuts at: 460 1303 1399 4222 4907 460
Size: 843 96 2823 685 2728
Fragments arranged by size:
2823 2728 843 685 96
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7175
PvuII CAG'CTG
Cuts at: 250 4600 4960 250
Size: 4350 360 2465
Fragments arranged by size:
4350 2465 360
RsrII CG'GwC_CG
Cuts at: 5370 5370
Size: 7175
SacI G_AGCT'C
Cuts at: 4189 4189
Size: 7175
SalI G'TCGA_C
Cuts at: 4212 4212
Size: 7175
SapI GCTCTTCn'nnn_
Cuts at: 2424 3751 5198 5408 2424
Size: 1327 1447 210 4191
Fragments arranged by size:
4191 1447 1327 210
SbfI CC_TGCA'GG
Cuts at: 4222 4222
Size: 7175
ScaI AGT'ACT
Cuts at: 1712 6692 1712
Size: 4980 2195
SexAI A'CCwGG_T
Cuts at: 3322 3322
Size: 7175
SfcI C'TryA_G
Cuts at: 33 456 705 1299 1395 4218 4903 5896 6087
Size: 423 249 594 96 2823 685 993 191
Cuts at: 6087 33
Size: 1121
Fragments arranged by size:
2823 1121 993 685 594 423 249 191
96
SfoI GGC'GCC
Cuts at: 4607 4855 4607
Size: 248 6927
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7175
SmaI CCC'GGG
Cuts at: 3583 4197 3583
Size: 614 6561
SmlI C'TyrA_G
Cuts at: 847 1411 3198 4330 5245 5737 5999 847
Size: 564 1787 1132 915 492 262 2023
Fragments arranged by size:
2023 1787 1132 915 564 492 262
SnaBI TAC'GTA
Cuts at: 4167 4167
Size: 7175
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7175
SphI G_CATG'C
Cuts at: 4228 4728 4228
Size: 500 6675
SspI AAT'ATT
Cuts at: 324 1359 324
Size: 1035 6140
StuI AGG'CCT
Cuts at: 3723 3723
Size: 7175
StyI C'CwwG_G
Cuts at: 136 2532 2822 3632 4309 4663 136
Size: 2396 290 810 677 354 2648
Fragments arranged by size:
2648 2396 810 677 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 494 736 2726 3029 3543 3589 4758 4891 5350
Size: 242 1990 303 514 46 1169 133 459
Cuts at: 5350 5559 494
Size: 209 2110
Fragments arranged by size:
2110 1990 1169 514 459 303 242 209
133 46
TatI w'GTAC_w
Cuts at: 879 1710 6690 879
Size: 831 4980 1364
Fragments arranged by size:
4980 1364 831
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 279 311 6692 6724 279
Size: 32 6381 32 730
Fragments arranged by size:
6381 730 32 32
Tth111I GACn'n_nGTC
Cuts at: 988 3070 4972 988
Size: 2082 1902 3191
Fragments arranged by size:
3191 2082 1902
XbaI T'CTAG_A
Cuts at: 2787 4206 2787
Size: 1419 5756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1239 3201 3639 3696 4125 4670 1239
Size: 1962 438 57 429 545 3744
Fragments arranged by size:
3744 1962 545 438 429 57
XmaI C'CCGG_G
Cuts at: 3581 4195 3581
Size: 614 6561
ZraI GAC'GTC
Cuts at: 555 1803 555
Size: 1248 5927
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI ApaI AscI AvrII BaeI
BcgI BmgBI BmtI BsiWI BsmI BsmBI BssHII BstXI
BstZ17I ClaI CspCI FseI FspAI MfeI NheI NotI
NruI PacI PsiI PspOMI PspXI SacII SanDI SfiI
SgrAI SrfI SwaI XhoI XmnI