Restriction map for
pF1KSDA0757
(Circular) (Six-base) MAPSORT of: pF1KSDA0757.seq Check: 4294 from: 1 to: 4699
pF1KSDA0757 4699 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1546 1546
Size: 4699
AccI GT'mk_AC
Cuts at: 496 1735 496
Size: 1239 3460
Acc65I G'GTAC_C
Cuts at: 1102 1713 1102
Size: 611 4088
AclI AA'CG_TT
Cuts at: 4539 4539
Size: 4699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 949 2522 2954 3703 3971 375
Size: 574 1573 432 749 268 1103
Fragments arranged by size:
1573 1103 749 574 432 268
AfeI AGC'GCT
Cuts at: 1237 1315 1581 1237
Size: 78 266 4355
Fragments arranged by size:
4355 266 78
AflIII A'CryG_T
Cuts at: 2049 3155 4132 2049
Size: 1106 977 2616
Fragments arranged by size:
2616 1106 977
AgeI A'CCGG_T
Cuts at: 3087 3087
Size: 4699
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 123 155 1639 1671 123
Size: 32 1484 32 3151
Fragments arranged by size:
3151 1484 32 32
AlwNI CAG_nnn'CTG
Cuts at: 905 1147 2002 3571 905
Size: 242 855 1569 2033
Fragments arranged by size:
2033 1569 855 242
ApaI G_GGCC'C
Cuts at: 475 475
Size: 4699
ApaLI G'TGCA_C
Cuts at: 3469 3469
Size: 4699
ApoI r'AATT_y
Cuts at: 154 445 1701 4052 4308 154
Size: 291 1256 2351 256 545
Fragments arranged by size:
2351 1256 545 291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4699
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4699
AvaI C'yCGr_G
Cuts at: 876 1717 876
Size: 841 3858
BamHI G'GATC_C
Cuts at: 291 1722 291
Size: 1431 3268
BanI G'GyrC_C
Cuts at: 385 1102 1525 1713 2127 2375 2410 385
Size: 717 423 188 414 248 35 2674
Fragments arranged by size:
2674 717 423 414 248 188 35
BanII G_rGCy'C
Cuts at: 343 378 475 584 623 1711 2741 343
Size: 35 97 109 39 1088 1030 2301
Fragments arranged by size:
2301 1088 1030 109 97 39 35
BbeI G_GCGC'C
Cuts at: 389 1529 2131 2379 389
Size: 1140 602 248 2709
Fragments arranged by size:
2709 1140 602 248
BbsI GAAGACnn'nnnn_
Cuts at: 937 937
Size: 4699
BbvCI CC'TCA_GC
Cuts at: 1576 1576
Size: 4699
BciVI GTATCCnnnnn_n'
Cuts at: 141 201 2595 3358 4699 141
Size: 60 2394 763 1341 141
Fragments arranged by size:
2394 1341 763 141 60
BclI T'GATC_A
Cuts at: 2217 2217
Size: 4699
BglI GCCn_nnn'nGGC
Cuts at: 1402 1402
Size: 4699
BglII A'GATC_T
Cuts at: 2212 4251 2212
Size: 2039 2660
BlpI GC'TnA_GC
Cuts at: 274 355 1809 274
Size: 81 1454 3164
Fragments arranged by size:
3164 1454 81
Bme1580I G_kGCm'C
Cuts at: 475 562 2322 2415 3473 475
Size: 87 1760 93 1058 1701
Fragments arranged by size:
1760 1701 1058 93 87
BmgBI CAC'GTC
Cuts at: 1128 1128
Size: 4699
BmrI ACTGGGnnnn_n'
Cuts at: 131 915 1539 1909 1990 2325 4471 131
Size: 784 624 370 81 335 2146 359
Fragments arranged by size:
2146 784 624 370 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 829 3064 829
Size: 2235 2464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 580 799 1325 421
Size: 159 219 526 3795
Fragments arranged by size:
3795 526 219 159
Bpu10I CC'TnA_GC
Cuts at: 529 551 1576 529
Size: 22 1025 3652
Fragments arranged by size:
3652 1025 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 1873 2752 3246 3544 970
Size: 903 879 494 298 2125
Fragments arranged by size:
2125 903 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 583 615 4403 583
Size: 32 3788 879
Fragments arranged by size:
3788 879 32
BsaAI yAC'GTr
Cuts at: 183 1689 2050 183
Size: 1506 361 2832
Fragments arranged by size:
2832 1506 361
BsaBI GATnn'nnATC
Cuts at: 6 2211 4250 6
Size: 2205 2039 455
Fragments arranged by size:
2205 2039 455
BsaHI Gr'CG_yC
Cuts at: 386 752 1526 1568 2128 2376 3080 4576 386
Size: 366 774 42 560 248 704 1496 509
Fragments arranged by size:
1496 774 704 560 509 366 248 42
BsaWI w'CCGG_w
Cuts at: 1886 2407 3087 3361 3508 3919 1886
Size: 521 680 274 147 411 2666
Fragments arranged by size:
2666 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1323 1353 1450 1480 1323
Size: 30 97 30 4542
Fragments arranged by size:
4542 97 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 351 1290 351
Size: 939 3760
BseYI C'CCAG_C
Cuts at: 555 1075 1354 1366 1661 2122 2880 3459 3955
Size: 520 279 12 295 461 758 579 496
Cuts at: 3955 555
Size: 1299
Fragments arranged by size:
1299 758 579 520 496 461 295 279
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 265 364 746 1524 1603 205
Size: 60 99 382 778 79 3301
Fragments arranged by size:
3301 778 382 99 79 60
BsiEI CG_ry'CG
Cuts at: 104 1189 1449 2239 2285 3495 104
Size: 1085 260 790 46 1210 1308
Fragments arranged by size:
1308 1210 1085 790 260 46
BsiHKAI G_wGCw'C
Cuts at: 343 584 623 785 1711 2489 2679 3473 343
Size: 241 39 162 926 778 190 794 1569
Fragments arranged by size:
1569 926 794 778 241 190 162 39
BsmBI CGTCTCn'nnnn_
Cuts at: 397 397
Size: 4699
Bsp1286I G_dGCh'C
Cuts at: 343 378 475 562 584 623 785 1711 2322
Size: 35 97 87 22 39 162 926 611
Cuts at: 2322 2415 2489 2679 2741 3473 343
Size: 93 74 190 62 732 1569
Fragments arranged by size:
1569 926 732 611 190 162 97 93
87 74 62 39 35 22
BspEI T'CCGG_A
Cuts at: 3919 3919
Size: 4699
BspHI T'CATG_A
Cuts at: 1383 3875 1383
Size: 2492 2207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1546 1747 1961 2263 2644 1546
Size: 201 214 302 381 3601
Fragments arranged by size:
3601 381 302 214 201
BsrBI CCG'CTC
Cuts at: 50 834 2989 3043 4529 4694 50
Size: 784 2155 54 1486 165 55
Fragments arranged by size:
2155 1486 784 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2609 2609
Size: 4699
BsrFI r'CCGG_y
Cuts at: 2695 2876 3087 2695
Size: 181 211 4307
Fragments arranged by size:
4307 211 181
BsrGI T'GTAC_A
Cuts at: 1136 1679 1136
Size: 543 4156
BssSI C'ACGA_G
Cuts at: 583 803 1675 2968 3328 583
Size: 220 872 1293 360 1954
Fragments arranged by size:
1954 1293 872 360 220
BstAPI GCAn_nnn'nTGC
Cuts at: 2074 2074
Size: 4699
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4699
BstXI CCAn_nnnn'nTGG
Cuts at: 144 144
Size: 4699
BstYI r'GATC_y
Cuts at: 1 291 1131 1722 2189 2212 2547 2793 3807
Size: 290 840 591 467 23 335 246 1014
Cuts at: 3807 3893 3905 4251 1
Size: 86 12 346 449
Fragments arranged by size:
1014 840 591 467 449 346 335 290
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 433 1025 1041 1398 125
Size: 308 592 16 357 3426
Fragments arranged by size:
3426 592 357 308 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 254 2647 2828 2991 254
Size: 2393 181 163 1962
Fragments arranged by size:
2393 1962 181 163
BtsI GCAGTG_nn'
Cuts at: 560 729 1940 560
Size: 169 1211 3319
Fragments arranged by size:
3319 1211 169
ClaI AT'CG_AT
Cuts at: 303 303
Size: 4699
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 953 988 953
Size: 35 4664
DraI TTT'AAA
Cuts at: 1696 1696
Size: 4699
DraIII CAC_nnn'GTG
Cuts at: 288 1547 288
Size: 1259 3440
DrdI GACnn_nn'nnGTC
Cuts at: 2403 3263 2403
Size: 860 3839
EaeI y'GGCC_r
Cuts at: 629 824 839 1028 1186 2282 2456 2847 2874
Size: 195 15 189 158 1096 174 391 27
Cuts at: 2874 4221 629
Size: 1347 1107
Fragments arranged by size:
1347 1107 1096 391 195 189 174 158
27 15
EagI C'GGCC_G
Cuts at: 1186 2282 1186
Size: 1096 3603
EarI CTCTTCn'nnn_
Cuts at: 427 503 2720 2930 427
Size: 76 2217 210 2196
Fragments arranged by size:
2217 2196 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 991 1248 2905 3217 3363 4509 991
Size: 257 1657 312 146 1146 1181
Fragments arranged by size:
1657 1181 1146 312 257 146
EcoICRI GAG'CTC
Cuts at: 341 582 621 1709 341
Size: 241 39 1088 3331
Fragments arranged by size:
3331 1088 241 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 421 580 799 949 1325 2522 2954 3703
Size: 46 159 219 150 376 1197 432 749
Cuts at: 3703 3971 375
Size: 268 1103
Fragments arranged by size:
1197 1103 749 432 376 268 219 159
150 46
EcoNI CCTnn'n_nnAGG
Cuts at: 283 283
Size: 4699
EcoO109I rG'GnC_Cy
Cuts at: 472 1440 1836 472
Size: 968 396 3335
Fragments arranged by size:
3335 968 396
EcoRI G'AATT_C
Cuts at: 445 1701 445
Size: 1256 3443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 183 2716 183
Size: 2533 2166
FspI TGC'GCA
Cuts at: 2478 2478
Size: 4699
HaeII r_GCGC'y
Cuts at: 389 1239 1317 1361 1529 1583 2131 2379 3403
Size: 850 78 44 168 54 548 248 1024
Cuts at: 3403 4382 389
Size: 979 706
Fragments arranged by size:
1024 979 850 706 548 248 168 78
54 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1204 1236 2539 2569 2571 2601 1204
Size: 32 1303 30 2 30 3302
Fragments arranged by size:
3302 1303 32 30 30 2
HincII GTy'rAC
Cuts at: 497 1736 497
Size: 1239 3460
KasI G'GCGC_C
Cuts at: 385 1525 2127 2375 385
Size: 1140 602 248 2709
Fragments arranged by size:
2709 1140 602 248
KpnI G_GTAC'C
Cuts at: 1106 1717 1106
Size: 611 4088
MluI A'CGCG_T
Cuts at: 4132 4132
Size: 4699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3370 3554 3370
Size: 184 4515
MscI TGG'CCA
Cuts at: 631 1030 2458 631
Size: 399 1428 2872
Fragments arranged by size:
2872 1428 399
MslI CAynn'nnrTG
Cuts at: 2686 2813 2686
Size: 127 4572
MspA1I CmG'CkG
Cuts at: 228 1502 1644 1808 2122 2482 3497 3742 228
Size: 1274 142 164 314 360 1015 245 1185
Fragments arranged by size:
1274 1185 1015 360 314 245 164 142
NaeI GCC'GGC
Cuts at: 2878 2878
Size: 4699
NarI GG'CG_CC
Cuts at: 386 1526 2128 2376 386
Size: 1140 602 248 2709
Fragments arranged by size:
2709 1140 602 248
NcoI C'CATG_G
Cuts at: 125 1025 125
Size: 900 3799
NgoMIV G'CCGG_C
Cuts at: 2876 2876
Size: 4699
NruI TCG'CGA
Cuts at: 3060 3060
Size: 4699
NspI r_CATG'y
Cuts at: 399 1750 2250 3159 399
Size: 1351 500 909 1939
Fragments arranged by size:
1939 1351 909 500
PasI CC'CwG_GG
Cuts at: 1154 1154
Size: 4699
PciI A'CATG_T
Cuts at: 3155 3155
Size: 4699
PmeI GTTT'AAAC
Cuts at: 1696 1696
Size: 4699
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3838 3870 3838
Size: 32 4667
PspOMI G'GGCC_C
Cuts at: 471 471
Size: 4699
PspXI vC'TCGA_Gb
Cuts at: 876 876
Size: 4699
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 172 204 172
Size: 32 4667
PstI C_TGCA'G
Cuts at: 1297 1744 2429 1297
Size: 447 685 3567
Fragments arranged by size:
3567 685 447
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4699
PvuII CAG'CTG
Cuts at: 1644 2122 2482 1644
Size: 478 360 3861
Fragments arranged by size:
3861 478 360
RsrII CG'GwC_CG
Cuts at: 2892 2892
Size: 4699
SacI G_AGCT'C
Cuts at: 343 584 623 1711 343
Size: 241 39 1088 3331
Fragments arranged by size:
3331 1088 241 39
SalI G'TCGA_C
Cuts at: 495 1734 495
Size: 1239 3460
SapI GCTCTTCn'nnn_
Cuts at: 2720 2930 2720
Size: 210 4489
SbfI CC_TGCA'GG
Cuts at: 1744 1744
Size: 4699
ScaI AGT'ACT
Cuts at: 4216 4216
Size: 4699
SfcI C'TryA_G
Cuts at: 33 1293 1740 2425 3420 3611 33
Size: 1260 447 685 995 191 1121
Fragments arranged by size:
1260 1121 995 685 447 191
SfoI GGC'GCC
Cuts at: 387 1527 2129 2377 387
Size: 1140 602 248 2709
Fragments arranged by size:
2709 1140 602 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4699
SmaI CCC'GGG
Cuts at: 1719 1719
Size: 4699
SmlI C'TyrA_G
Cuts at: 876 985 1852 2767 3261 3523 876
Size: 109 867 915 494 262 2052
Fragments arranged by size:
2052 915 867 494 262 109
SnaBI TAC'GTA
Cuts at: 1689 1689
Size: 4699
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4699
SphI G_CATG'C
Cuts at: 399 1750 2250 399
Size: 1351 500 2848
Fragments arranged by size:
2848 1351 500
StuI AGG'CCT
Cuts at: 934 1375 934
Size: 441 4258
StyI C'CwwG_G
Cuts at: 125 842 1025 1831 2185 125
Size: 717 183 806 354 2639
Fragments arranged by size:
2639 806 717 354 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1558 1675 2280 2413 2872 3083 1558
Size: 117 605 133 459 211 3174
Fragments arranged by size:
3174 605 459 211 133 117
TatI w'GTAC_w
Cuts at: 1136 1679 4214 1136
Size: 543 2535 1621
Fragments arranged by size:
2535 1621 543
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1323 1355 1450 1456 1482 1488 4216 4248 1323
Size: 32 95 6 26 6 2728 32 1774
Fragments arranged by size:
2728 1774 95 32 32 26 6 6
Tth111I GACn'n_nGTC
Cuts at: 2494 2494
Size: 4699
XbaI T'CTAG_A
Cuts at: 1728 1728
Size: 4699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 804 2192 804
Size: 1388 3311
XhoI C'TCGA_G
Cuts at: 876 876
Size: 4699
XmaI C'CCGG_G
Cuts at: 1717 1717
Size: 4699
XmnI GAAnn'nnTTC
Cuts at: 445 445
Size: 4699
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PasI PciI
PmeI PpiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BcgI BfrBI BmtI BsiWI BsmI BssHII BstEII BstZ17I
Bsu36I EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI SacII SanDI SexAI SfiI SgrAI SrfI
SspI SwaI ZraI