Restriction map for
pF1KSDA0755
(Circular) (Six-base) MAPSORT of: pF1KSDA0755.seq Check: 8349 from: 1 to: 6235
pF1KSDA0755 6235 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 1567 1567
Size: 6235
AccI GT'mk_AC
Cuts at: 2898 3271 2898
Size: 373 5862
Acc65I G'GTAC_C
Cuts at: 3249 3249
Size: 6235
AclI AA'CG_TT
Cuts at: 6075 6075
Size: 6235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 1907 3026 4058 4490 5239 5507 1540
Size: 367 1119 1032 432 749 268 2268
Fragments arranged by size:
2268 1119 1032 749 432 367 268
AflIII A'CryG_T
Cuts at: 1232 1760 2804 2903 3585 4691 5668 1232
Size: 528 1044 99 682 1106 977 1799
Fragments arranged by size:
1799 1106 1044 977 682 528 99
AgeI A'CCGG_T
Cuts at: 4623 4623
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 2740 2740
Size: 6235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 617 649 617
Size: 32 6203
AlwNI CAG_nnn'CTG
Cuts at: 3135 3538 5107 3135
Size: 403 1569 4263
Fragments arranged by size:
4263 1569 403
ApaLI G'TGCA_C
Cuts at: 5005 5005
Size: 6235
ApoI r'AATT_y
Cuts at: 1538 2546 3237 5588 5844 1538
Size: 1008 691 2351 256 1929
Fragments arranged by size:
2351 1929 1008 691 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6235
AvaI C'yCGr_G
Cuts at: 3253 3253
Size: 6235
BamHI G'GATC_C
Cuts at: 216 903 3258 216
Size: 687 2355 3193
Fragments arranged by size:
3193 2355 687
BanI G'GyrC_C
Cuts at: 670 3249 3663 3911 3946 670
Size: 2579 414 248 35 2959
Fragments arranged by size:
2959 2579 414 248 35
BanII G_rGCy'C
Cuts at: 348 713 3247 4277 348
Size: 365 2534 1030 2306
Fragments arranged by size:
2534 2306 1030 365
BbeI G_GCGC'C
Cuts at: 3667 3915 3667
Size: 248 5987
BbsI GAAGACnn'nnnn_
Cuts at: 1353 1528 2348 2406 3149 1353
Size: 175 820 58 743 4439
Fragments arranged by size:
4439 820 743 175 58
BbvCI CC'TCA_GC
Cuts at: 170 2078 170
Size: 1908 4327
BciVI GTATCCnnnnn_n'
Cuts at: 4131 4894 6235 4131
Size: 763 1341 4131
Fragments arranged by size:
4131 1341 763
BclI T'GATC_A
Cuts at: 3753 3753
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 261 261
Size: 6235
BglII A'GATC_T
Cuts at: 2705 3203 3748 5787 2705
Size: 498 545 2039 3153
Fragments arranged by size:
3153 2039 545 498
BlpI GC'TnA_GC
Cuts at: 1103 3345 1103
Size: 2242 3993
Bme1580I G_kGCm'C
Cuts at: 209 1240 3858 3951 5009 209
Size: 1031 2618 93 1058 1435
Fragments arranged by size:
2618 1435 1058 1031 93
BmgBI CAC'GTC
Cuts at: 1091 2055 1091
Size: 964 5271
BmrI ACTGGGnnnn_n'
Cuts at: 732 3445 3526 3861 6007 732
Size: 2713 81 335 2146 960
Fragments arranged by size:
2713 2146 960 335 81
BmtI G_CTAG'C
Cuts at: 2446 2446
Size: 6235
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1448 4600 1448
Size: 3152 3083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 2108 875
Size: 1233 5002
Bpu10I CC'TnA_GC
Cuts at: 170 2078 170
Size: 1908 4327
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 614 3409 4288 4782 5080 614
Size: 2795 879 494 298 1769
Fragments arranged by size:
2795 1769 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5939 5939
Size: 6235
BsaAI yAC'GTr
Cuts at: 144 3225 3586 144
Size: 3081 361 2793
Fragments arranged by size:
3081 2793 361
BsaBI GATnn'nnATC
Cuts at: 6 3747 5786 6
Size: 3741 2039 455
Fragments arranged by size:
3741 2039 455
BsaHI Gr'CG_yC
Cuts at: 1564 3664 3912 4616 6112 1564
Size: 2100 248 704 1496 1687
Fragments arranged by size:
2100 1687 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2585 3422 3943 4623 4897 5044 5455 2585
Size: 837 521 680 274 147 411 3365
Fragments arranged by size:
3365 837 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 219 249 953 983 219
Size: 30 704 30 5471
Fragments arranged by size:
5471 704 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 527 578 713 716 974 527
Size: 51 135 3 258 5788
Fragments arranged by size:
5788 258 135 51 3
BseYI C'CCAG_C
Cuts at: 479 483 1637 2064 3658 4416 4995 5491 479
Size: 4 1154 427 1594 758 579 496 1223
Fragments arranged by size:
1594 1223 1154 758 579 496 427 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 848 419
Size: 429 5806
BsiEI CG_ry'CG
Cuts at: 104 3775 3821 5031 104
Size: 3671 46 1210 1308
Fragments arranged by size:
3671 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 348 3247 4025 4215 5009 348
Size: 2899 778 190 794 1574
Fragments arranged by size:
2899 1574 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1555 1555
Size: 6235
Bsp1286I G_dGCh'C
Cuts at: 209 348 713 1240 3247 3858 3951 4025 4215
Size: 139 365 527 2007 611 93 74 190
Cuts at: 4215 4277 5009 209
Size: 62 732 1435
Fragments arranged by size:
2007 1435 732 611 527 365 190 139
93 74 62
BspEI T'CCGG_A
Cuts at: 5455 5455
Size: 6235
BspHI T'CATG_A
Cuts at: 1457 2744 5411 1457
Size: 1287 2667 2281
Fragments arranged by size:
2667 2281 1287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2841 3283 3497 3799 4180 2841
Size: 442 214 302 381 4896
Fragments arranged by size:
4896 442 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4525 4579 6065 6230 50
Size: 4475 54 1486 165 55
Fragments arranged by size:
4475 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 577 2995 4145 577
Size: 2418 1150 2667
Fragments arranged by size:
2667 2418 1150
BsrFI r'CCGG_y
Cuts at: 4231 4412 4623 4231
Size: 181 211 5843
Fragments arranged by size:
5843 211 181
BsrGI T'GTAC_A
Cuts at: 695 1066 2657 695
Size: 371 1591 4273
Fragments arranged by size:
4273 1591 371
BssSI C'ACGA_G
Cuts at: 4504 4864 4504
Size: 360 5875
BstAPI GCAn_nnn'nTGC
Cuts at: 1220 3610 1220
Size: 2390 3845
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6235
BstEII G'GTnAC_C
Cuts at: 358 358
Size: 6235
BstXI CCAn_nnnn'nTGG
Cuts at: 331 2757 331
Size: 2426 3809
BstYI r'GATC_y
Cuts at: 1 216 625 903 995 1384 2705 3203 3258
Size: 215 409 278 92 389 1321 498 55
Cuts at: 3258 3725 3748 4083 4329 5343 5429 5441 5787
Size: 467 23 335 246 1014 86 12 346
Cuts at: 5787 1
Size: 449
Fragments arranged by size:
1321 1014 498 467 449 409 389 346
335 278 246 215 92 86 55 23
12
BtgI C'CryG_G
Cuts at: 2309 2750 2309
Size: 441 5794
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4183 4364 4527 4183
Size: 181 163 5891
Fragments arranged by size:
5891 181 163
BtsI GCAGTG_nn'
Cuts at: 2309 3476 2309
Size: 1167 5068
ClaI AT'CG_AT
Cuts at: 2288 2288
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1559 1594 1559
Size: 35 6200
DraI TTT'AAA
Cuts at: 2508 3232 2508
Size: 724 5511
DrdI GACnn_nn'nnGTC
Cuts at: 3939 4799 3939
Size: 860 5375
EaeI y'GGCC_r
Cuts at: 1988 3818 3992 4383 4410 5757 1988
Size: 1830 174 391 27 1347 2466
Fragments arranged by size:
2466 1830 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3818 3818
Size: 6235
EarI CTCTTCn'nnn_
Cuts at: 761 1544 1553 1618 2032 3171 4256 4466 761
Size: 783 9 65 414 1139 1085 210 2530
Fragments arranged by size:
2530 1139 1085 783 414 210 65 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 869 4441 4753 4899 6045 869
Size: 3572 312 146 1146 1059
Fragments arranged by size:
3572 1146 1059 312 146
EcoICRI GAG'CTC
Cuts at: 346 3245 346
Size: 2899 3336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 875 1540 1907 2108 3026 4058 4490 5239 5507
Size: 665 367 201 918 1032 432 749 268
Cuts at: 5507 875
Size: 1603
Fragments arranged by size:
1603 1032 918 749 665 432 367 268
201
EcoNI CCTnn'n_nnAGG
Cuts at: 179 758 179
Size: 579 5656
EcoO109I rG'GnC_Cy
Cuts at: 262 304 382 454 550 859 3372 262
Size: 42 78 72 96 309 2513 3125
Fragments arranged by size:
3125 2513 309 96 78 72 42
EcoRI G'AATT_C
Cuts at: 3237 3237
Size: 6235
EcoRV GAT'ATC
Cuts at: 784 1273 784
Size: 489 5746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4252 4252
Size: 6235
FspI TGC'GCA
Cuts at: 4014 4014
Size: 6235
HaeII r_GCGC'y
Cuts at: 3667 3915 4939 5918 3667
Size: 248 1024 979 3984
Fragments arranged by size:
3984 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 697 729 861 893 895 927 980 1012 2044
Size: 32 132 32 2 32 53 32 1032
Cuts at: 2044 2076 2342 2374 2400 2432 2460 2492 2690
Size: 32 266 32 26 32 28 32 198
Cuts at: 2690 2722 2726 2758 4075 4105 4107 4137 697
Size: 32 4 32 1317 30 2 30 2795
Fragments arranged by size:
2795 1317 1032 266 198 132 53 32
32 32 32 32 32 32 32 32
32 30 30 28 26 4 2 2
HincII GTy'rAC
Cuts at: 133 396 1707 3272 133
Size: 263 1311 1565 3096
Fragments arranged by size:
3096 1565 1311 263
KasI G'GCGC_C
Cuts at: 3663 3911 3663
Size: 248 5987
KpnI G_GTAC'C
Cuts at: 3253 3253
Size: 6235
MfeI C'AATT_G
Cuts at: 3089 3089
Size: 6235
MluI A'CGCG_T
Cuts at: 5668 5668
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 262 1347 1647 1670 1724 3081 3150 4906 5090
Size: 1085 300 23 54 1357 69 1756 184
Cuts at: 5090 262
Size: 1407
Fragments arranged by size:
1756 1407 1357 1085 300 184 69 54
23
MscI TGG'CCA
Cuts at: 1990 3994 1990
Size: 2004 4231
MslI CAynn'nnrTG
Cuts at: 2749 2755 2989 4222 4349 2749
Size: 6 234 1233 127 4635
Fragments arranged by size:
4635 1233 234 127 6
MspA1I CmG'CkG
Cuts at: 483 2739 3213 3344 3658 4018 5033 5278 483
Size: 2256 474 131 314 360 1015 245 1440
Fragments arranged by size:
2256 1440 1015 474 360 314 245 131
NaeI GCC'GGC
Cuts at: 4414 4414
Size: 6235
NarI GG'CG_CC
Cuts at: 3664 3912 3664
Size: 248 5987
NcoI C'CATG_G
Cuts at: 2750 2750
Size: 6235
NdeI CA'TA_TG
Cuts at: 1507 2570 1507
Size: 1063 5172
NgoMIV G'CCGG_C
Cuts at: 4412 4412
Size: 6235
NheI G'CTAG_C
Cuts at: 2442 2442
Size: 6235
NruI TCG'CGA
Cuts at: 4596 4596
Size: 6235
NspI r_CATG'y
Cuts at: 418 1236 1764 2582 2907 3286 3786 4695 418
Size: 818 528 818 325 379 500 909 1958
Fragments arranged by size:
1958 909 818 818 528 500 379 325
PciI A'CATG_T
Cuts at: 1232 1760 2903 4691 1232
Size: 528 1143 1788 2776
Fragments arranged by size:
2776 1788 1143 528
PflMI CCAn_nnn'nTGG
Cuts at: 316 771 1329 2574 316
Size: 455 558 1245 3977
Fragments arranged by size:
3977 1245 558 455
PfoI T'CCnGG_A
Cuts at: 315 2540 315
Size: 2225 4010
PmeI GTTT'AAAC
Cuts at: 3232 3232
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5374 5406 5374
Size: 32 6203
PpuMI rG'GwC_Cy
Cuts at: 304 454 550 859 304
Size: 150 96 309 5680
Fragments arranged by size:
5680 309 150 96
PsiI TTA'TAA
Cuts at: 2456 2456
Size: 6235
PstI C_TGCA'G
Cuts at: 2434 2613 3280 3965 2434
Size: 179 667 685 4704
Fragments arranged by size:
4704 685 667 179
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6235
PvuII CAG'CTG
Cuts at: 483 2739 3213 3658 4018 483
Size: 2256 474 445 360 2700
Fragments arranged by size:
2700 2256 474 445 360
RsrII CG'GwC_CG
Cuts at: 4428 4428
Size: 6235
SacI G_AGCT'C
Cuts at: 348 3247 348
Size: 2899 3336
SalI G'TCGA_C
Cuts at: 3270 3270
Size: 6235
SanDI GG'GwC_CC
Cuts at: 304 550 304
Size: 246 5989
SapI GCTCTTCn'nnn_
Cuts at: 1544 3171 4256 4466 1544
Size: 1627 1085 210 3313
Fragments arranged by size:
3313 1627 1085 210
SbfI CC_TGCA'GG
Cuts at: 3280 3280
Size: 6235
ScaI AGT'ACT
Cuts at: 5752 5752
Size: 6235
SfcI C'TryA_G
Cuts at: 33 1013 2430 2609 3276 3961 4956 5147 33
Size: 980 1417 179 667 685 995 191 1121
Fragments arranged by size:
1417 1121 995 980 685 667 191 179
SfoI GGC'GCC
Cuts at: 3665 3913 3665
Size: 248 5987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6235
SmaI CCC'GGG
Cuts at: 3255 3255
Size: 6235
SmlI C'TyrA_G
Cuts at: 629 3388 4303 4797 5059 629
Size: 2759 915 494 262 1805
Fragments arranged by size:
2759 1805 915 494 262
SnaBI TAC'GTA
Cuts at: 3225 3225
Size: 6235
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6235
SphI G_CATG'C
Cuts at: 3286 3786 3286
Size: 500 5735
SspI AAT'ATT
Cuts at: 2879 2954 2879
Size: 75 6160
StuI AGG'CCT
Cuts at: 185 1228 185
Size: 1043 5192
StyI C'CwwG_G
Cuts at: 770 1035 1991 2750 2946 3367 3721 770
Size: 265 956 759 196 421 354 3284
Fragments arranged by size:
3284 956 759 421 354 265 196
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 493 663 763 2743 3816 3949 4408 4619 493
Size: 170 100 1980 1073 133 459 211 2109
Fragments arranged by size:
2109 1980 1073 459 211 170 133 100
TatI w'GTAC_w
Cuts at: 695 1066 2657 2685 2821 5750 695
Size: 371 1591 28 136 2929 1180
Fragments arranged by size:
2929 1591 1180 371 136 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 219 251 2580 2612 5752 5784 219
Size: 32 2329 32 3140 32 670
Fragments arranged by size:
3140 2329 670 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4030 4030
Size: 6235
XbaI T'CTAG_A
Cuts at: 3264 3264
Size: 6235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 553 1998 3728 553
Size: 1445 1730 3060
Fragments arranged by size:
3060 1730 1445
XmaI C'CCGG_G
Cuts at: 3253 3253
Size: 6235
XmnI GAAnn'nnTTC
Cuts at: 1538 1855 1538
Size: 317 5918
ZraI GAC'GTC
Cuts at: 1565 1565
Size: 6235
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NruI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI ApaI AscI AvrII
BaeI BcgI BfrBI BsiWI BsmI BssHII BstZ17I Bsu36I
DraIII FseI FspAI HindIII HpaI NotI NsiI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SexAI
SfiI SgrAI SrfI SwaI XhoI