Restriction map for
pF1KSDA0745
(Circular) (Six-base) MAPSORT of: pF1KSDA0745.seq Check: 8341 from: 1 to: 5854
pF1KSDA0745 5854 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 536 536
Size: 5854
AccI GT'mk_AC
Cuts at: 2890 2890
Size: 5854
Acc65I G'GTAC_C
Cuts at: 1862 2868 1862
Size: 1006 4848
AclI AA'CG_TT
Cuts at: 5694 5694
Size: 5854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 710 2080 2116 2363 3677 4109 4858 5126
Size: 212 1370 36 247 1314 432 749 268
Cuts at: 5126 498
Size: 1226
Fragments arranged by size:
1370 1314 1226 749 432 268 247 212
36
AflIII A'CryG_T
Cuts at: 949 3204 4310 5287 949
Size: 2255 1106 977 1516
Fragments arranged by size:
2255 1516 1106 977
AgeI A'CCGG_T
Cuts at: 4242 4242
Size: 5854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 336 336
Size: 5854
AlwNI CAG_nnn'CTG
Cuts at: 147 309 1002 2526 2763 3157 4726 147
Size: 162 693 1524 237 394 1569 1275
Fragments arranged by size:
1569 1524 1275 693 394 237 162
ApaLI G'TGCA_C
Cuts at: 953 4624 953
Size: 3671 2183
ApoI r'AATT_y
Cuts at: 232 2802 2856 5207 5463 232
Size: 2570 54 2351 256 623
Fragments arranged by size:
2570 2351 623 256 54
AseI AT'TA_AT
Cuts at: 20 611 20
Size: 591 5263
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5854
AvaI C'yCGr_G
Cuts at: 2872 2872
Size: 5854
BamHI G'GATC_C
Cuts at: 2877 2877
Size: 5854
BanI G'GyrC_C
Cuts at: 1862 2868 3282 3530 3565 1862
Size: 1006 414 248 35 4151
Fragments arranged by size:
4151 1006 414 248 35
BanII G_rGCy'C
Cuts at: 1859 2866 3896 1859
Size: 1007 1030 3817
Fragments arranged by size:
3817 1030 1007
BbeI G_GCGC'C
Cuts at: 3286 3534 3286
Size: 248 5606
BbsI GAAGACnn'nnnn_
Cuts at: 504 952 1344 2564 504
Size: 448 392 1220 3794
Fragments arranged by size:
3794 1220 448 392
BciVI GTATCCnnnnn_n'
Cuts at: 3750 4513 5854 3750
Size: 763 1341 3750
Fragments arranged by size:
3750 1341 763
BclI T'GATC_A
Cuts at: 3372 3372
Size: 5854
BglII A'GATC_T
Cuts at: 1256 3367 5406 1256
Size: 2111 2039 1704
Fragments arranged by size:
2111 2039 1704
BlpI GC'TnA_GC
Cuts at: 1231 2236 2964 1231
Size: 1005 728 4121
Fragments arranged by size:
4121 1005 728
Bme1580I G_kGCm'C
Cuts at: 957 3477 3570 4628 957
Size: 2520 93 1058 2183
Fragments arranged by size:
2520 2183 1058 93
BmgBI CAC'GTC
Cuts at: 950 2313 950
Size: 1363 4491
BmrI ACTGGGnnnn_n'
Cuts at: 297 487 981 1395 3064 3145 3480 5626 297
Size: 190 494 414 1669 81 335 2146 525
Fragments arranged by size:
2146 1669 525 494 414 335 190 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4219 4219
Size: 5854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 900 1147 1921 193
Size: 707 247 774 4126
Fragments arranged by size:
4126 774 707 247
Bpu10I CC'TnA_GC
Cuts at: 256 1345 256
Size: 1089 4765
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 769 1879 2050 3028 3907 4401 4699 769
Size: 1110 171 978 879 494 298 1924
Fragments arranged by size:
1924 1110 978 879 494 298 171
BsaI GGTCTCn'nnnn_
Cuts at: 1327 1745 5558 1327
Size: 418 3813 1623
Fragments arranged by size:
3813 1623 418
BsaAI yAC'GTr
Cuts at: 2434 2844 3205 2434
Size: 410 361 5083
Fragments arranged by size:
5083 410 361
BsaBI GATnn'nnATC
Cuts at: 6 1631 3366 5405 6
Size: 1625 1735 2039 455
Fragments arranged by size:
2039 1735 1625 455
BsaHI Gr'CG_yC
Cuts at: 533 1130 1599 3283 3531 4235 5731 533
Size: 597 469 1684 248 704 1496 656
Fragments arranged by size:
1684 1496 704 656 597 469 248
BsaWI w'CCGG_w
Cuts at: 2664 3041 3562 4242 4516 4663 5074 2664
Size: 377 521 680 274 147 411 3444
Fragments arranged by size:
3444 680 521 411 377 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 265 949 1594 2185 265
Size: 684 645 591 3934
Fragments arranged by size:
3934 684 645 591
BseYI C'CCAG_C
Cuts at: 1928 3277 4035 4614 5110 1928
Size: 1349 758 579 496 2672
Fragments arranged by size:
2672 1349 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 883 985 1063 1297 2339 2470 160
Size: 723 102 78 234 1042 131 3544
Fragments arranged by size:
3544 1042 723 234 131 102 78
BsiEI CG_ry'CG
Cuts at: 104 418 2478 3394 3440 4650 104
Size: 314 2060 916 46 1210 1308
Fragments arranged by size:
2060 1308 1210 916 314 46
BsiHKAI G_wGCw'C
Cuts at: 398 957 1163 1520 1859 2866 3644 3834 4628
Size: 559 206 357 339 1007 778 190 794
Cuts at: 4628 398
Size: 1624
Fragments arranged by size:
1624 1007 794 778 559 357 339 206
190
BsmI GAATG_Cn'
Cuts at: 2770 2770
Size: 5854
BsmBI CGTCTCn'nnnn_
Cuts at: 1606 2144 2146 1606
Size: 538 2 5314
Fragments arranged by size:
5314 538 2
Bsp1286I G_dGCh'C
Cuts at: 398 957 1163 1520 1859 2866 3477 3570 3644
Size: 559 206 357 339 1007 611 93 74
Cuts at: 3644 3834 3896 4628 398
Size: 190 62 732 1624
Fragments arranged by size:
1624 1007 732 611 559 357 339 206
190 93 74 62
BspEI T'CCGG_A
Cuts at: 5074 5074
Size: 5854
BspHI T'CATG_A
Cuts at: 1796 5030 1796
Size: 3234 2620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2902 3116 3418 3799 2902
Size: 214 302 381 4957
Fragments arranged by size:
4957 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4144 4198 5684 5849 50
Size: 4094 54 1486 165 55
Fragments arranged by size:
4094 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1034 2832 3764 1034
Size: 1798 932 3124
Fragments arranged by size:
3124 1798 932
BsrFI r'CCGG_y
Cuts at: 3850 4031 4242 3850
Size: 181 211 5462
Fragments arranged by size:
5462 211 181
BsrGI T'GTAC_A
Cuts at: 856 856
Size: 5854
BssSI C'ACGA_G
Cuts at: 961 1509 4123 4483 961
Size: 548 2614 360 2332
Fragments arranged by size:
2614 2332 548 360
BstAPI GCAn_nnn'nTGC
Cuts at: 768 1650 2381 2493 2682 3229 768
Size: 882 731 112 189 547 3393
Fragments arranged by size:
3393 882 731 547 189 112
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5854
BstYI r'GATC_y
Cuts at: 1 1256 2877 3344 3367 3702 3948 4962 5048
Size: 1255 1621 467 23 335 246 1014 86
Cuts at: 5048 5060 5406 1
Size: 12 346 449
Fragments arranged by size:
1621 1255 1014 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1133 1337 2366 2531 125
Size: 1008 204 1029 165 3448
Fragments arranged by size:
3448 1029 1008 204 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3802 3983 4146 3802
Size: 181 163 5510
Fragments arranged by size:
5510 181 163
BtsI GCAGTG_nn'
Cuts at: 1046 1091 3095 1046
Size: 45 2004 3805
Fragments arranged by size:
3805 2004 45
DraI TTT'AAA
Cuts at: 2851 2851
Size: 5854
DraIII CAC_nnn'GTG
Cuts at: 2406 2406
Size: 5854
DrdI GACnn_nn'nnGTC
Cuts at: 3558 4418 3558
Size: 860 4994
EaeI y'GGCC_r
Cuts at: 415 1095 1223 3437 3611 4002 4029 5376 415
Size: 680 128 2214 174 391 27 1347 893
Fragments arranged by size:
2214 1347 893 680 391 174 128 27
EagI C'GGCC_G
Cuts at: 415 3437 415
Size: 3022 2832
EarI CTCTTCn'nnn_
Cuts at: 666 1956 1978 2337 2422 2440 3875 4085 666
Size: 1290 22 359 85 18 1435 210 2435
Fragments arranged by size:
2435 1435 1290 359 210 85 22 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 4060 4372 4518 5664 145
Size: 3915 312 146 1146 335
Fragments arranged by size:
3915 1146 335 312 146
EcoICRI GAG'CTC
Cuts at: 1857 2864 1857
Size: 1007 4847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 498 710 900 1147 1921 2080 2116 2363
Size: 305 212 190 247 774 159 36 247
Cuts at: 2363 3677 4109 4858 5126 193
Size: 1314 432 749 268 921
Fragments arranged by size:
1314 921 774 749 432 305 268 247
247 212 190 159 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2272 2464 2272
Size: 192 5662
EcoO109I rG'GnC_Cy
Cuts at: 1925 2034 2991 1925
Size: 109 957 4788
Fragments arranged by size:
4788 957 109
EcoRI G'AATT_C
Cuts at: 2802 2856 2802
Size: 54 5800
EcoRV GAT'ATC
Cuts at: 690 690
Size: 5854
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 311 1809 3871 311
Size: 1498 2062 2294
Fragments arranged by size:
2294 2062 1498
FspI TGC'GCA
Cuts at: 3633 3633
Size: 5854
HaeII r_GCGC'y
Cuts at: 1016 3286 3534 4558 5537 1016
Size: 2270 248 1024 979 1333
Fragments arranged by size:
2270 1333 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 1073 1105 3694 3724 3726 3756 826
Size: 32 215 32 2589 30 2 30 2924
Fragments arranged by size:
2924 2589 215 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2787 2891 2787
Size: 104 5750
HindIII A'AGCT_T
Cuts at: 331 1474 331
Size: 1143 4711
KasI G'GCGC_C
Cuts at: 3282 3530 3282
Size: 248 5606
KpnI G_GTAC'C
Cuts at: 1866 2872 1866
Size: 1006 4848
MfeI C'AATT_G
Cuts at: 1850 1850
Size: 5854
MluI A'CGCG_T
Cuts at: 5287 5287
Size: 5854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1552 1574 1866 4525 4709 1552
Size: 22 292 2659 184 2697
Fragments arranged by size:
2697 2659 292 184 22
MscI TGG'CCA
Cuts at: 1225 3613 1225
Size: 2388 3466
MslI CAynn'nnrTG
Cuts at: 1039 3841 3968 1039
Size: 2802 127 2925
Fragments arranged by size:
2925 2802 127
MspA1I CmG'CkG
Cuts at: 177 878 915 1167 2427 2963 3277 3637 4652
Size: 701 37 252 1260 536 314 360 1015
Cuts at: 4652 4897 177
Size: 245 1134
Fragments arranged by size:
1260 1134 1015 701 536 360 314 252
245 37
NaeI GCC'GGC
Cuts at: 4033 4033
Size: 5854
NarI GG'CG_CC
Cuts at: 3283 3531 3283
Size: 248 5606
NcoI C'CATG_G
Cuts at: 125 1133 1337 2366 125
Size: 1008 204 1029 3613
Fragments arranged by size:
3613 1029 1008 204
NdeI CA'TA_TG
Cuts at: 2282 2282
Size: 5854
NgoMIV G'CCGG_C
Cuts at: 4031 4031
Size: 5854
NruI TCG'CGA
Cuts at: 671 4215 671
Size: 3544 2310
NspI r_CATG'y
Cuts at: 323 1566 2905 3405 4314 323
Size: 1243 1339 500 909 1863
Fragments arranged by size:
1863 1339 1243 909 500
PciI A'CATG_T
Cuts at: 4310 4310
Size: 5854
PflMI CCAn_nnn'nTGG
Cuts at: 309 878 2596 309
Size: 569 1718 3567
Fragments arranged by size:
3567 1718 569
PmeI GTTT'AAAC
Cuts at: 2851 2851
Size: 5854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4993 5025 4993
Size: 32 5822
PpuMI rG'GwC_Cy
Cuts at: 1925 2034 1925
Size: 109 5745
PshAI GACnn'nnGTC
Cuts at: 1339 1339
Size: 5854
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1630 1662 1630
Size: 32 5822
PstI C_TGCA'G
Cuts at: 2177 2899 3584 2177
Size: 722 685 4447
Fragments arranged by size:
4447 722 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5854
PvuII CAG'CTG
Cuts at: 177 878 915 1167 2427 3277 3637 177
Size: 701 37 252 1260 850 360 2394
Fragments arranged by size:
2394 1260 850 701 360 252 37
RsrII CG'GwC_CG
Cuts at: 4047 4047
Size: 5854
SacI G_AGCT'C
Cuts at: 1859 2866 1859
Size: 1007 4847
SalI G'TCGA_C
Cuts at: 2889 2889
Size: 5854
SapI GCTCTTCn'nnn_
Cuts at: 3875 4085 3875
Size: 210 5644
SbfI CC_TGCA'GG
Cuts at: 2899 2899
Size: 5854
ScaI AGT'ACT
Cuts at: 1384 5371 1384
Size: 3987 1867
SfcI C'TryA_G
Cuts at: 33 1006 1420 2173 2562 2895 3580 4575 4766
Size: 973 414 753 389 333 685 995 191
Cuts at: 4766 33
Size: 1121
Fragments arranged by size:
1121 995 973 753 685 414 389 333
191
SfoI GGC'GCC
Cuts at: 3284 3532 3284
Size: 248 5606
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5854
SmaI CCC'GGG
Cuts at: 2874 2874
Size: 5854
SmlI C'TyrA_G
Cuts at: 784 1894 2065 3007 3922 4416 4678 784
Size: 1110 171 942 915 494 262 1960
Fragments arranged by size:
1960 1110 942 915 494 262 171
SnaBI TAC'GTA
Cuts at: 2844 2844
Size: 5854
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5854
SphI G_CATG'C
Cuts at: 2905 3405 2905
Size: 500 5354
StyI C'CwwG_G
Cuts at: 125 224 1133 1337 2366 2986 3340 125
Size: 99 909 204 1029 620 354 2639
Fragments arranged by size:
2639 1029 909 620 354 204 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 643 2770 3435 3568 4027 4238 643
Size: 2127 665 133 459 211 2259
Fragments arranged by size:
2259 2127 665 459 211 133
TatI w'GTAC_w
Cuts at: 856 1382 1879 1890 5369 856
Size: 526 497 11 3479 1341
Fragments arranged by size:
3479 1341 526 497 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 615 647 5371 5403 615
Size: 32 4724 32 1066
Fragments arranged by size:
4724 1066 32 32
Tth111I GACn'n_nGTC
Cuts at: 508 3649 508
Size: 3141 2713
XbaI T'CTAG_A
Cuts at: 2883 2883
Size: 5854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3347 3347
Size: 5854
XmaI C'CCGG_G
Cuts at: 2872 2872
Size: 5854
XmnI GAAnn'nnTTC
Cuts at: 1247 1913 1247
Size: 666 5188
ZraI GAC'GTC
Cuts at: 534 534
Size: 5854
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NspI PciI PflMI PmeI PpiI PpuMI
PshAI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI BsaXI
BsiWI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
FseI FspAI HpaI NheI NotI NsiI PacI PasI
PfoI PmlI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SrfI SspI StuI SwaI XhoI