Restriction map for
pF1KSDA0741
(Circular) (Six-base) MAPSORT of: pF1KSDA0741.seq Check: 3095 from: 1 to: 6799
pF1KSDA0741 6799 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2098 2098
Size: 6799
AccI GT'mk_AC
Cuts at: 3835 3835
Size: 6799
Acc65I G'GTAC_C
Cuts at: 2586 3813 2586
Size: 1227 5572
AclI AA'CG_TT
Cuts at: 6639 6639
Size: 6799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1031 1739 3173 3784 4622 5054 5803 6071 1031
Size: 708 1434 611 838 432 749 268 1759
Fragments arranged by size:
1759 1434 838 749 708 611 432 268
AflIII A'CryG_T
Cuts at: 2088 4149 5255 6232 2088
Size: 2061 1106 977 2655
Fragments arranged by size:
2655 2061 1106 977
AgeI A'CCGG_T
Cuts at: 5187 5187
Size: 6799
AleI CACnn'nnGTG
Cuts at: 2575 3498 2575
Size: 923 5876
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1676 1708 1676
Size: 32 6767
AlwNI CAG_nnn'CTG
Cuts at: 1897 4102 5671 1897
Size: 2205 1569 3025
Fragments arranged by size:
3025 2205 1569
ApaI G_GGCC'C
Cuts at: 2813 2813
Size: 6799
ApaLI G'TGCA_C
Cuts at: 5569 5569
Size: 6799
ApoI r'AATT_y
Cuts at: 394 666 700 949 2063 2184 2482 2515 3112
Size: 272 34 249 1114 121 298 33 597
Cuts at: 3112 3290 3373 3516 3801 6152 6408 394
Size: 178 83 143 285 2351 256 785
Fragments arranged by size:
2351 1114 785 597 298 285 272 256
249 178 143 121 83 34 33
AseI AT'TA_AT
Cuts at: 20 2159 20
Size: 2139 4660
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6799
AvaI C'yCGr_G
Cuts at: 2724 3437 3817 2724
Size: 713 380 5706
Fragments arranged by size:
5706 713 380
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 980 1013 980
Size: 33 6766
BamHI G'GATC_C
Cuts at: 3822 3822
Size: 6799
BanI G'GyrC_C
Cuts at: 2586 2734 3813 4227 4475 4510 2586
Size: 148 1079 414 248 35 4875
Fragments arranged by size:
4875 1079 414 248 148 35
BanII G_rGCy'C
Cuts at: 1891 2019 2330 2813 3811 4841 1891
Size: 128 311 483 998 1030 3849
Fragments arranged by size:
3849 1030 998 483 311 128
BbeI G_GCGC'C
Cuts at: 4231 4479 4231
Size: 248 6551
BbsI GAAGACnn'nnnn_
Cuts at: 161 1861 2062 3651 161
Size: 1700 201 1589 3309
Fragments arranged by size:
3309 1700 1589 201
BciVI GTATCCnnnnn_n'
Cuts at: 4695 5458 6799 4695
Size: 763 1341 4695
Fragments arranged by size:
4695 1341 763
BclI T'GATC_A
Cuts at: 2756 4317 2756
Size: 1561 5238
BfrBI ATG'CAT
Cuts at: 2316 2316
Size: 6799
BglII A'GATC_T
Cuts at: 4312 6351 4312
Size: 2039 4760
BlpI GC'TnA_GC
Cuts at: 1172 3909 1172
Size: 2737 4062
Bme1580I G_kGCm'C
Cuts at: 2737 2813 3137 4422 4515 5573 2737
Size: 76 324 1285 93 1058 3963
Fragments arranged by size:
3963 1285 1058 324 93 76
BmrI ACTGGGnnnn_n'
Cuts at: 3097 3154 4009 4090 4425 6571 3097
Size: 57 855 81 335 2146 3325
Fragments arranged by size:
3325 2146 855 335 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5164 5164
Size: 6799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 2819 2959 1013
Size: 1806 140 4853
Fragments arranged by size:
4853 1806 140
Bpu10I CC'TnA_GC
Cuts at: 1409 2909 1409
Size: 1500 5299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 317 3973 4852 5346 5644 317
Size: 3656 879 494 298 1472
Fragments arranged by size:
3656 1472 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 6503 6503
Size: 6799
BsaAI yAC'GTr
Cuts at: 3789 4150 3789
Size: 361 6438
BsaBI GATnn'nnATC
Cuts at: 6 1916 3409 4311 6350 6
Size: 1910 1493 902 2039 455
Fragments arranged by size:
2039 1910 1493 902 455
BsaHI Gr'CG_yC
Cuts at: 785 4228 4476 5180 6676 785
Size: 3443 248 704 1496 908
Fragments arranged by size:
3443 1496 908 704 248
BsaWI w'CCGG_w
Cuts at: 3986 4507 5187 5461 5608 6019 3986
Size: 521 680 274 147 411 4766
Fragments arranged by size:
4766 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1572 1602 3056 3086 1572
Size: 30 1454 30 5285
Fragments arranged by size:
5285 1454 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 634 1678 1738 1741 1744 1747 1789 1798 2503
Size: 1044 60 3 3 3 42 9 705
Cuts at: 2503 2837 634
Size: 334 4596
Fragments arranged by size:
4596 1044 705 334 60 42 9 3
3 3
BseYI C'CCAG_C
Cuts at: 4222 4980 5559 6055 4222
Size: 758 579 496 4966
Fragments arranged by size:
4966 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3320 2582
Size: 738 6061
BsiEI CG_ry'CG
Cuts at: 104 4339 4385 5595 104
Size: 4235 46 1210 1308
Fragments arranged by size:
4235 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1891 2700 3811 4589 4779 5573 1891
Size: 809 1111 778 190 794 3117
Fragments arranged by size:
3117 1111 809 794 778 190
BsmI GAATG_Cn'
Cuts at: 2222 2541 2222
Size: 319 6480
Bsp1286I G_dGCh'C
Cuts at: 1891 2019 2330 2700 2737 2813 3137 3811 4422
Size: 128 311 370 37 76 324 674 611
Cuts at: 4422 4515 4589 4779 4841 5573 1891
Size: 93 74 190 62 732 3117
Fragments arranged by size:
3117 732 674 611 370 324 311 190
128 93 76 74 62 37
BspEI T'CCGG_A
Cuts at: 6019 6019
Size: 6799
BspHI T'CATG_A
Cuts at: 5975 5975
Size: 6799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2098 3463 3847 4061 4363 4744 2098
Size: 1365 384 214 302 381 4153
Fragments arranged by size:
4153 1365 384 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5089 5143 6629 6794 50
Size: 5039 54 1486 165 55
Fragments arranged by size:
5039 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2288 4709 2288
Size: 2421 4378
BsrFI r'CCGG_y
Cuts at: 3686 4795 4976 5187 3686
Size: 1109 181 211 5298
Fragments arranged by size:
5298 1109 211 181
BsrGI T'GTAC_A
Cuts at: 2091 2091
Size: 6799
BssSI C'ACGA_G
Cuts at: 5068 5428 5068
Size: 360 6439
BstAPI GCAn_nnn'nTGC
Cuts at: 4174 4174
Size: 6799
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6799
BstYI r'GATC_y
Cuts at: 1 2562 3441 3822 4289 4312 4647 4893 5907
Size: 2561 879 381 467 23 335 246 1014
Cuts at: 5907 5993 6005 6351 1
Size: 86 12 346 449
Fragments arranged by size:
2561 1014 879 467 449 381 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 6799
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4747 4928 5091 4747
Size: 181 163 6455
Fragments arranged by size:
6455 181 163
BtsI GCAGTG_nn'
Cuts at: 371 1809 4040 371
Size: 1438 2231 3130
Fragments arranged by size:
3130 2231 1438
DraI TTT'AAA
Cuts at: 2453 3796 2453
Size: 1343 5456
DraIII CAC_nnn'GTG
Cuts at: 2683 2683
Size: 6799
DrdI GACnn_nn'nnGTC
Cuts at: 2340 4503 5363 2340
Size: 2163 860 3776
Fragments arranged by size:
3776 2163 860
EaeI y'GGCC_r
Cuts at: 1652 4382 4556 4947 4974 6321 1652
Size: 2730 174 391 27 1347 2130
Fragments arranged by size:
2730 2130 1347 391 174 27
EagI C'GGCC_G
Cuts at: 4382 4382
Size: 6799
EarI CTCTTCn'nnn_
Cuts at: 383 890 1007 1195 1202 1253 1444 1571 1715
Size: 507 117 188 7 51 191 127 144
Cuts at: 1715 1736 1766 1775 2681 3730 4820 5030 383
Size: 21 30 9 906 1049 1090 210 2152
Fragments arranged by size:
2152 1090 1049 906 507 210 191 188
144 127 117 51 30 21 9 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5005 5317 5463 6609 5005
Size: 312 146 1146 5195
Fragments arranged by size:
5195 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1889 3809 1889
Size: 1920 4879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1031 1739 2819 2959 3173 3784 4622 5054
Size: 18 708 1080 140 214 611 838 432
Cuts at: 5054 5803 6071 1013
Size: 749 268 1741
Fragments arranged by size:
1741 1080 838 749 708 611 432 268
214 140 18
EcoO109I rG'GnC_Cy
Cuts at: 751 1129 2809 3936 751
Size: 378 1680 1127 3614
Fragments arranged by size:
3614 1680 1127 378
EcoRI G'AATT_C
Cuts at: 394 3801 394
Size: 3407 3392
EcoRV GAT'ATC
Cuts at: 297 297
Size: 6799
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 297 1360 1408 4816 297
Size: 1063 48 3408 2280
Fragments arranged by size:
3408 2280 1063 48
FspI TGC'GCA
Cuts at: 4578 4578
Size: 6799
HaeII r_GCGC'y
Cuts at: 4231 4479 5503 6482 4231
Size: 248 1024 979 4548
Fragments arranged by size:
4548 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 475 507 772 804 1572 1604 2771 2803 3056
Size: 32 265 32 768 32 1167 32 253
Cuts at: 3056 3088 4639 4669 4671 4701 475
Size: 32 1551 30 2 30 2573
Fragments arranged by size:
2573 1551 1167 768 265 253 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2648 3525 3836 2648
Size: 877 311 5611
Fragments arranged by size:
5611 877 311
HindIII A'AGCT_T
Cuts at: 1174 1174
Size: 6799
HpaI GTT'AAC
Cuts at: 2648 2648
Size: 6799
KasI G'GCGC_C
Cuts at: 4227 4475 4227
Size: 248 6551
KpnI G_GTAC'C
Cuts at: 2590 3817 2590
Size: 1227 5572
MfeI C'AATT_G
Cuts at: 2336 2336
Size: 6799
MluI A'CGCG_T
Cuts at: 6232 6232
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3179 5470 5654 3179
Size: 2291 184 4324
Fragments arranged by size:
4324 2291 184
MscI TGG'CCA
Cuts at: 1654 4558 1654
Size: 2904 3895
MslI CAynn'nnrTG
Cuts at: 2087 2575 2606 3498 4786 4913 2087
Size: 488 31 892 1288 127 3973
Fragments arranged by size:
3973 1288 892 488 127 31
MspA1I CmG'CkG
Cuts at: 1036 3908 4222 4582 5597 5842 1036
Size: 2872 314 360 1015 245 1993
Fragments arranged by size:
2872 1993 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3688 4978 3688
Size: 1290 5509
NarI GG'CG_CC
Cuts at: 4228 4476 4228
Size: 248 6551
NcoI C'CATG_G
Cuts at: 125 125
Size: 6799
NgoMIV G'CCGG_C
Cuts at: 3686 4976 3686
Size: 1290 5509
NruI TCG'CGA
Cuts at: 5160 5160
Size: 6799
NsiI A_TGCA'T
Cuts at: 2318 2318
Size: 6799
NspI r_CATG'y
Cuts at: 2044 2092 3850 4350 5259 2044
Size: 48 1758 500 909 3584
Fragments arranged by size:
3584 1758 909 500 48
PciI A'CATG_T
Cuts at: 2088 5255 2088
Size: 3167 3632
PfoI T'CCnGG_A
Cuts at: 2211 2796 2211
Size: 585 6214
PmeI GTTT'AAAC
Cuts at: 3796 3796
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5938 5970 5938
Size: 32 6767
PpuMI rG'GwC_Cy
Cuts at: 751 1129 751
Size: 378 6421
PsiI TTA'TAA
Cuts at: 2978 2978
Size: 6799
PspOMI G'GGCC_C
Cuts at: 2809 2809
Size: 6799
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1547 1579 1547
Size: 32 6767
PstI C_TGCA'G
Cuts at: 198 1041 3844 4529 198
Size: 843 2803 685 2468
Fragments arranged by size:
2803 2468 843 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6799
PvuII CAG'CTG
Cuts at: 1036 4222 4582 1036
Size: 3186 360 3253
Fragments arranged by size:
3253 3186 360
RsrII CG'GwC_CG
Cuts at: 4992 4992
Size: 6799
SacI G_AGCT'C
Cuts at: 1891 3811 1891
Size: 1920 4879
SalI G'TCGA_C
Cuts at: 3834 3834
Size: 6799
SapI GCTCTTCn'nnn_
Cuts at: 2681 4820 5030 2681
Size: 2139 210 4450
Fragments arranged by size:
4450 2139 210
SbfI CC_TGCA'GG
Cuts at: 3844 3844
Size: 6799
ScaI AGT'ACT
Cuts at: 6316 6316
Size: 6799
SfcI C'TryA_G
Cuts at: 33 194 1037 1541 2741 3659 3840 4525 5520
Size: 161 843 504 1200 918 181 685 995
Cuts at: 5520 5711 33
Size: 191 1121
Fragments arranged by size:
1200 1121 995 918 843 685 504 191
181 161
SfoI GGC'GCC
Cuts at: 4229 4477 4229
Size: 248 6551
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6799
SmaI CCC'GGG
Cuts at: 2726 3819 2726
Size: 1093 5706
SmlI C'TyrA_G
Cuts at: 332 3437 3952 4867 5361 5623 332
Size: 3105 515 915 494 262 1508
Fragments arranged by size:
3105 1508 915 515 494 262
SnaBI TAC'GTA
Cuts at: 3789 3789
Size: 6799
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6799
SphI G_CATG'C
Cuts at: 3850 4350 3850
Size: 500 6299
StuI AGG'CCT
Cuts at: 2834 2834
Size: 6799
StyI C'CwwG_G
Cuts at: 125 164 188 3931 4285 125
Size: 39 24 3743 354 2639
Fragments arranged by size:
3743 2639 354 39 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3510 3645 4380 4513 4972 5183 3510
Size: 135 735 133 459 211 5126
Fragments arranged by size:
5126 735 459 211 135 133
TatI w'GTAC_w
Cuts at: 524 2091 3422 6314 524
Size: 1567 1331 2892 1009
Fragments arranged by size:
2892 1567 1331 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2127 2159 6316 6348 2127
Size: 32 4157 32 2578
Fragments arranged by size:
4157 2578 32 32
Tth111I GACn'n_nGTC
Cuts at: 4594 4594
Size: 6799
XbaI T'CTAG_A
Cuts at: 712 2067 3828 712
Size: 1355 1761 3683
Fragments arranged by size:
3683 1761 1355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4292 4292
Size: 6799
XhoI C'TCGA_G
Cuts at: 3437 3437
Size: 6799
XmaI C'CCGG_G
Cuts at: 2724 3817 2724
Size: 1093 5706
XmnI GAAnn'nnTTC
Cuts at: 666 666
Size: 6799
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PciI PfoI
PmeI PpiI PpuMI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BbvCI
BcgI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI EcoNI FseI FspAI
NdeI NheI NotI PacI PasI PflMI PmlI PshAI
PspXI SacII SanDI SexAI SfiI SgrAI SrfI SspI
SwaI ZraI