Restriction map for
pF1KSDA0736
(Circular) (Six-base) MAPSORT of: pF1KSDA0736.seq Check: 2338 from: 1 to: 5363
pF1KSDA0736 5363 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2331 2331
Size: 5363
AccI GT'mk_AC
Cuts at: 758 1916 2401 758
Size: 1158 485 3720
Fragments arranged by size:
3720 1158 485
Acc65I G'GTAC_C
Cuts at: 1373 2379 1373
Size: 1006 4357
AclI AA'CG_TT
Cuts at: 5203 5203
Size: 5363
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 2338 3188 3620 4367 4635 1267
Size: 1071 850 432 747 268 1995
Fragments arranged by size:
1995 1071 850 747 432 268
AfeI AGC'GCT
Cuts at: 702 1381 702
Size: 679 4684
AflIII A'CryG_T
Cuts at: 713 1754 2135 2715 3819 4796 713
Size: 1041 381 580 1104 977 1280
Fragments arranged by size:
1280 1104 1041 977 580 381
AgeI A'CCGG_T
Cuts at: 3751 3751
Size: 5363
AleI CACnn'nnGTG
Cuts at: 1579 1579
Size: 5363
AlwNI CAG_nnn'CTG
Cuts at: 442 828 2346 2668 4235 442
Size: 386 1518 322 1567 1570
Fragments arranged by size:
1570 1567 1518 386 322
ApaLI G'TGCA_C
Cuts at: 4133 4133
Size: 5363
ApoI r'AATT_y
Cuts at: 1425 2367 4716 4972 1425
Size: 942 2349 256 1816
Fragments arranged by size:
2349 1816 942 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5363
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5363
AvaI C'yCGr_G
Cuts at: 431 1588 2333 2383 431
Size: 1157 745 50 3411
Fragments arranged by size:
3411 1157 745 50
AvrII C'CTAG_G
Cuts at: 1404 1404
Size: 5363
BamHI G'GATC_C
Cuts at: 2388 2388
Size: 5363
BanI G'GyrC_C
Cuts at: 474 1373 1903 2379 2793 3041 3076 474
Size: 899 530 476 414 248 35 2761
Fragments arranged by size:
2761 899 530 476 414 248 35
BanII G_rGCy'C
Cuts at: 1176 1198 2377 3407 1176
Size: 22 1179 1030 3132
Fragments arranged by size:
3132 1179 1030 22
BbeI G_GCGC'C
Cuts at: 478 2797 3045 478
Size: 2319 248 2796
Fragments arranged by size:
2796 2319 248
BbsI GAAGACnn'nnnn_
Cuts at: 849 945 1122 849
Size: 96 177 5090
Fragments arranged by size:
5090 177 96
BbvCI CC'TCA_GC
Cuts at: 2283 2283
Size: 5363
BciVI GTATCCnnnnn_n'
Cuts at: 3261 4022 5363 3261
Size: 761 1341 3261
Fragments arranged by size:
3261 1341 761
BclI T'GATC_A
Cuts at: 2883 2883
Size: 5363
BglI GCCn_nnn'nGGC
Cuts at: 1171 2099 1171
Size: 928 4435
BglII A'GATC_T
Cuts at: 401 2878 4915 401
Size: 2477 2037 849
Fragments arranged by size:
2477 2037 849
BlpI GC'TnA_GC
Cuts at: 2475 2475
Size: 5363
Bme1580I G_kGCm'C
Cuts at: 2988 3081 4137 2988
Size: 93 1056 4214
Fragments arranged by size:
4214 1056 93
BmgBI CAC'GTC
Cuts at: 1884 2136 1884
Size: 252 5111
BmrI ACTGGGnnnn_n'
Cuts at: 302 2575 2656 2991 5135 302
Size: 2273 81 335 2144 530
Fragments arranged by size:
2273 2144 530 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 802 3730 802
Size: 2928 2435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1143 1534 2001 506
Size: 637 391 467 3868
Fragments arranged by size:
3868 637 467 391
Bpu10I CC'TnA_GC
Cuts at: 182 1289 2283 182
Size: 1107 994 3262
Fragments arranged by size:
3262 1107 994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 1419 1672 2539 3418 3910 4208 522
Size: 897 253 867 879 492 298 1677
Fragments arranged by size:
1677 897 879 867 492 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 137 2323 5067 137
Size: 2186 2744 433
Fragments arranged by size:
2744 2186 433
BsaAI yAC'GTr
Cuts at: 2355 2716 2355
Size: 361 5002
BsaBI GATnn'nnATC
Cuts at: 6 1348 2230 2877 4914 6
Size: 1342 882 647 2037 455
Fragments arranged by size:
2037 1342 882 647 455
BsaHI Gr'CG_yC
Cuts at: 475 2794 3042 3744 5240 475
Size: 2319 248 702 1496 598
Fragments arranged by size:
2319 1496 702 598 248
BsaWI w'CCGG_w
Cuts at: 2552 3073 3751 4025 4172 4583 2552
Size: 521 678 274 147 411 3332
Fragments arranged by size:
3332 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 810 840 810
Size: 30 5333
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 289 292 341 361 289
Size: 3 49 20 5291
Fragments arranged by size:
5291 49 20 3
BseYI C'CCAG_C
Cuts at: 697 810 1382 2220 2788 3546 4123 4619 697
Size: 113 572 838 568 758 577 496 1441
Fragments arranged by size:
1441 838 758 577 572 568 496 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1755 1840 2249 1755
Size: 85 409 4869
Fragments arranged by size:
4869 409 85
BsiEI CG_ry'CG
Cuts at: 104 612 2905 2951 4159 104
Size: 508 2293 46 1208 1308
Fragments arranged by size:
2293 1308 1208 508 46
BsiHKAI G_wGCw'C
Cuts at: 2377 3155 3345 4137 2377
Size: 778 190 792 3603
Fragments arranged by size:
3603 792 778 190
BsmI GAATG_Cn'
Cuts at: 425 2009 2130 2199 425
Size: 1584 121 69 3589
Fragments arranged by size:
3589 1584 121 69
Bsp1286I G_dGCh'C
Cuts at: 1176 1198 2377 2988 3081 3155 3345 3407 4137
Size: 22 1179 611 93 74 190 62 730
Cuts at: 4137 1176
Size: 2402
Fragments arranged by size:
2402 1179 730 611 190 93 74 62
22
BspEI T'CCGG_A
Cuts at: 4583 4583
Size: 5363
BspHI T'CATG_A
Cuts at: 4539 4539
Size: 5363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1242 1404 2331 2413 2627 2929 3310 1242
Size: 162 927 82 214 302 381 3295
Fragments arranged by size:
3295 927 381 302 214 162 82
BsrBI CCG'CTC
Cuts at: 50 459 3655 3709 5193 5358 50
Size: 409 3196 54 1484 165 55
Fragments arranged by size:
3196 1484 409 165 55 54
BsrDI GCAATG_nn'
Cuts at: 176 2111 3275 176
Size: 1935 1164 2264
Fragments arranged by size:
2264 1935 1164
BsrFI r'CCGG_y
Cuts at: 806 3361 3542 3751 806
Size: 2555 181 209 2418
Fragments arranged by size:
2555 2418 209 181
BssSI C'ACGA_G
Cuts at: 3634 3992 3634
Size: 358 5005
BstAPI GCAn_nnn'nTGC
Cuts at: 828 2346 2740 828
Size: 1518 394 3451
Fragments arranged by size:
3451 1518 394
BstBI TT'CG_AA
Cuts at: 110 3724 110
Size: 3614 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 213 213
Size: 5363
BstYI r'GATC_y
Cuts at: 1 401 1349 1637 2388 2855 2878 3213 3459
Size: 400 948 288 751 467 23 335 246
Cuts at: 3459 4471 4557 4569 4915 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 948 751 467 449 400 346 335
288 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1917 1917
Size: 5363
BtgI C'CryG_G
Cuts at: 125 162 606 125
Size: 37 444 4882
Fragments arranged by size:
4882 444 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3313 3494 3657 3313
Size: 181 163 5019
Fragments arranged by size:
5019 181 163
BtsI GCAGTG_nn'
Cuts at: 829 1562 1952 2606 829
Size: 733 390 654 3586
Fragments arranged by size:
3586 733 654 390
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2163 2198 2163
Size: 35 5328
DraI TTT'AAA
Cuts at: 2362 2362
Size: 5363
DrdI GACnn_nn'nnGTC
Cuts at: 3069 3927 3069
Size: 858 4505
EaeI y'GGCC_r
Cuts at: 203 603 609 2072 2948 3122 3513 3540 4885
Size: 400 6 1463 876 174 391 27 1345
Cuts at: 4885 203
Size: 681
Fragments arranged by size:
1463 1345 876 681 400 391 174 27
6
EagI C'GGCC_G
Cuts at: 609 2948 609
Size: 2339 3024
EarI CTCTTCn'nnn_
Cuts at: 125 907 1715 3386 3596 125
Size: 782 808 1671 210 1892
Fragments arranged by size:
1892 1671 808 782 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1531 3571 3881 4027 5173 1531
Size: 2040 310 146 1146 1721
Fragments arranged by size:
2040 1721 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2375 2375
Size: 5363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 506 1143 1267 1534 2001 2338 3188 3620 4367
Size: 637 124 267 467 337 850 432 747
Cuts at: 4367 4635 506
Size: 268 1234
Fragments arranged by size:
1234 850 747 637 467 432 337 268
267 124
EcoO109I rG'GnC_Cy
Cuts at: 222 335 517 1367 2502 222
Size: 113 182 850 1135 3083
Fragments arranged by size:
3083 1135 850 182 113
EcoRI G'AATT_C
Cuts at: 2367 2367
Size: 5363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3382 3382
Size: 5363
FspI TGC'GCA
Cuts at: 3144 3144
Size: 5363
HaeII r_GCGC'y
Cuts at: 478 652 704 1383 2797 3045 4067 5046 478
Size: 174 52 679 1414 248 1022 979 795
Fragments arranged by size:
1414 1022 979 795 679 248 174 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 810 842 926 958 1311 1339 1343 1371 1530
Size: 32 84 32 353 28 4 28 159
Cuts at: 1530 1552 1562 1584 1729 1761 3205 3235 3237
Size: 22 10 22 145 32 1444 30 2
Cuts at: 3237 3267 810
Size: 30 2906
Fragments arranged by size:
2906 1444 353 159 145 84 32 32
32 30 30 28 28 22 22 10
4 2
HincII GTy'rAC
Cuts at: 849 2141 2402 849
Size: 1292 261 3810
Fragments arranged by size:
3810 1292 261
KasI G'GCGC_C
Cuts at: 474 2793 3041 474
Size: 2319 248 2796
Fragments arranged by size:
2796 2319 248
KpnI G_GTAC'C
Cuts at: 1377 2383 1377
Size: 1006 4357
MluI A'CGCG_T
Cuts at: 4796 4796
Size: 5363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 749 755 796 1337 1607 4034 4218 749
Size: 6 41 541 270 2427 184 1894
Fragments arranged by size:
2427 1894 541 270 184 41 6
MscI TGG'CCA
Cuts at: 605 3124 605
Size: 2519 2844
MslI CAynn'nnrTG
Cuts at: 772 1468 1579 3352 3479 772
Size: 696 111 1773 127 2656
Fragments arranged by size:
2656 1773 696 127 111
MspA1I CmG'CkG
Cuts at: 1048 1121 2215 2287 2474 2788 3148 4161 4406
Size: 73 1094 72 187 314 360 1013 245
Cuts at: 4406 1048
Size: 2005
Fragments arranged by size:
2005 1094 1013 360 314 245 187 73
72
NaeI GCC'GGC
Cuts at: 3544 3544
Size: 5363
NarI GG'CG_CC
Cuts at: 475 2794 3042 475
Size: 2319 248 2796
Fragments arranged by size:
2796 2319 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5363
NgoMIV G'CCGG_C
Cuts at: 3542 3542
Size: 5363
NspI r_CATG'y
Cuts at: 202 717 744 1020 1272 1609 2416 2916 3823
Size: 515 27 276 252 337 807 500 907
Cuts at: 3823 202
Size: 1742
Fragments arranged by size:
1742 907 807 515 500 337 276 252
27
PasI CC'CwG_GG
Cuts at: 523 523
Size: 5363
PciI A'CATG_T
Cuts at: 713 3819 713
Size: 3106 2257
PflMI CCAn_nnn'nTGG
Cuts at: 1557 1557
Size: 5363
PfoI T'CCnGG_A
Cuts at: 236 2119 236
Size: 1883 3480
PmeI GTTT'AAAC
Cuts at: 2362 2362
Size: 5363
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1282 1314 4502 4534 1282
Size: 32 3188 32 2111
Fragments arranged by size:
3188 2111 32 32
PpuMI rG'GwC_Cy
Cuts at: 335 517 1367 335
Size: 182 850 4331
Fragments arranged by size:
4331 850 182
PstI C_TGCA'G
Cuts at: 2351 2410 3095 2351
Size: 59 685 4619
Fragments arranged by size:
4619 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5363
PvuII CAG'CTG
Cuts at: 1048 1121 2215 2287 2788 3148 1048
Size: 73 1094 72 501 360 3263
Fragments arranged by size:
3263 1094 501 360 73 72
RsrII CG'GwC_CG
Cuts at: 3558 3558
Size: 5363
SacI G_AGCT'C
Cuts at: 2377 2377
Size: 5363
SalI G'TCGA_C
Cuts at: 2400 2400
Size: 5363
SanDI GG'GwC_CC
Cuts at: 335 517 335
Size: 182 5181
SapI GCTCTTCn'nnn_
Cuts at: 3386 3596 3386
Size: 210 5153
SbfI CC_TGCA'GG
Cuts at: 2410 2410
Size: 5363
ScaI AGT'ACT
Cuts at: 1657 4880 1657
Size: 3223 2140
SexAI A'CCwGG_T
Cuts at: 1369 1369
Size: 5363
SfcI C'TryA_G
Cuts at: 33 2347 2406 3091 4084 4275 33
Size: 2314 59 685 993 191 1121
Fragments arranged by size:
2314 1121 993 685 191 59
SfoI GGC'GCC
Cuts at: 476 2795 3043 476
Size: 2319 248 2796
Fragments arranged by size:
2796 2319 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5363
SmaI CCC'GGG
Cuts at: 433 1590 2335 2385 433
Size: 1157 745 50 3411
Fragments arranged by size:
3411 1157 745 50
SmlI C'TyrA_G
Cuts at: 501 1398 1687 2518 3433 3925 4187 501
Size: 897 289 831 915 492 262 1677
Fragments arranged by size:
1677 915 897 831 492 289 262
SnaBI TAC'GTA
Cuts at: 2355 2355
Size: 5363
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5363
SphI G_CATG'C
Cuts at: 202 744 2416 2916 202
Size: 542 1672 500 2649
Fragments arranged by size:
2649 1672 542 500
SspI AAT'ATT
Cuts at: 247 247
Size: 5363
StuI AGG'CCT
Cuts at: 749 1234 749
Size: 485 4878
StyI C'CwwG_G
Cuts at: 125 1404 2258 2298 2497 2851 125
Size: 1279 854 40 199 354 2637
Fragments arranged by size:
2637 1279 854 354 199 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1070 1468 2283 2946 3079 3538 3747 1070
Size: 398 815 663 133 459 209 2686
Fragments arranged by size:
2686 815 663 459 398 209 133
TatI w'GTAC_w
Cuts at: 1655 1814 1846 4878 1655
Size: 159 32 3032 2140
Fragments arranged by size:
3032 2140 159 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1017 1049 4880 4912 1017
Size: 32 3831 32 1468
Fragments arranged by size:
3831 1468 32 32
Tth111I GACn'n_nGTC
Cuts at: 232 3160 232
Size: 2928 2435
XbaI T'CTAG_A
Cuts at: 2394 2394
Size: 5363
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2858 2858
Size: 5363
XmaI C'CCGG_G
Cuts at: 431 1588 2333 2383 431
Size: 1157 745 50 3411
Fragments arranged by size:
3411 1157 745 50
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI BaeI
BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SfiI
SgrAI SrfI SwaI XhoI XmnI ZraI