Restriction map for
pF1KSDA0722
(Circular) (Six-base) MAPSORT of: pF1KSDA0722.seq Check: 5299 from: 1 to: 6287
pF1KSDA0722 6287 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1388 1425 2916 1388
Size: 37 1491 4759
Fragments arranged by size:
4759 1491 37
AccI GT'mk_AC
Cuts at: 389 758 3325 389
Size: 369 2567 3351
Fragments arranged by size:
3351 2567 369
Acc65I G'GTAC_C
Cuts at: 635 3303 635
Size: 2668 3619
AclI AA'CG_TT
Cuts at: 6127 6127
Size: 6287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 2734 2857 3051 4112 4544 5291 5559 346
Size: 2388 123 194 1061 432 747 268 1074
Fragments arranged by size:
2388 1074 1061 747 432 268 194 123
AfeI AGC'GCT
Cuts at: 3001 3001
Size: 6287
AflII C'TTAA_G
Cuts at: 2233 2233
Size: 6287
AflIII A'CryG_T
Cuts at: 3639 4743 5720 3639
Size: 1104 977 4206
Fragments arranged by size:
4206 1104 977
AgeI A'CCGG_T
Cuts at: 4675 4675
Size: 6287
AleI CACnn'nnGTG
Cuts at: 1435 1435
Size: 6287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3113 3113
Size: 6287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3097 3129 3097
Size: 32 6255
AlwNI CAG_nnn'CTG
Cuts at: 300 768 810 1095 2049 2524 2982 3592 5159
Size: 468 42 285 954 475 458 610 1567
Cuts at: 5159 300
Size: 1428
Fragments arranged by size:
1567 1428 954 610 475 468 458 285
42
ApaI G_GGCC'C
Cuts at: 2155 2188 2431 2458 2504 2746 2155
Size: 33 243 27 46 242 5696
Fragments arranged by size:
5696 243 242 46 33 27
ApaLI G'TGCA_C
Cuts at: 5057 5057
Size: 6287
ApoI r'AATT_y
Cuts at: 3291 5640 5896 3291
Size: 2349 256 3682
Fragments arranged by size:
3682 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
AvaI C'yCGr_G
Cuts at: 694 857 1017 1148 1747 2265 2403 3307 694
Size: 163 160 131 599 518 138 904 3674
Fragments arranged by size:
3674 904 599 518 163 160 138 131
BamHI G'GATC_C
Cuts at: 3312 3312
Size: 6287
BanI G'GyrC_C
Cuts at: 199 496 635 748 780 1239 1405 2359 2493
Size: 297 139 113 32 459 166 954 134
Cuts at: 2493 3303 3717 3965 4000 199
Size: 810 414 248 35 2486
Fragments arranged by size:
2486 954 810 459 414 297 248 166
139 134 113 35 32
BanII G_rGCy'C
Cuts at: 134 1288 1939 1993 2155 2188 2349 2398 2431
Size: 1154 651 54 162 33 161 49 33
Cuts at: 2431 2458 2504 2531 2746 3301 4331 134
Size: 27 46 27 215 555 1030 2090
Fragments arranged by size:
2090 1154 1030 651 555 215 162 161
54 49 46 33 33 27 27
BbeI G_GCGC'C
Cuts at: 203 500 784 1243 1409 3721 3969 203
Size: 297 284 459 166 2312 248 2521
Fragments arranged by size:
2521 2312 459 297 284 248 166
BbsI GAAGACnn'nnnn_
Cuts at: 207 1108 2035 2376 207
Size: 901 927 341 4118
Fragments arranged by size:
4118 927 901 341
BbvCI CC'TCA_GC
Cuts at: 1235 2313 1235
Size: 1078 5209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 912 946 2551 2585 912
Size: 34 1605 34 4614
Fragments arranged by size:
4614 1605 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4185 4946 6287 4185
Size: 761 1341 4185
Fragments arranged by size:
4185 1341 761
BclI T'GATC_A
Cuts at: 3807 3807
Size: 6287
BglI GCCn_nnn'nGGC
Cuts at: 143 1117 1379 1538 2192 2462 2731 143
Size: 974 262 159 654 270 269 3699
Fragments arranged by size:
3699 974 654 270 269 262 159
BglII A'GATC_T
Cuts at: 3802 5839 3802
Size: 2037 4250
BlpI GC'TnA_GC
Cuts at: 454 1577 2794 3399 454
Size: 1123 1217 605 3342
Fragments arranged by size:
3342 1217 1123 605
Bme1580I G_kGCm'C
Cuts at: 1004 2012 2155 2188 2362 2431 2458 2504 2746
Size: 1008 143 33 174 69 27 46 242
Cuts at: 2746 3912 4005 5061 1004
Size: 1166 93 1056 2230
Fragments arranged by size:
2230 1166 1056 1008 242 174 143 93
69 46 33 27
BmgBI CAC'GTC
Cuts at: 1818 2694 1818
Size: 876 5411
BmrI ACTGGGnnnn_n'
Cuts at: 1238 1355 1417 1476 3499 3580 3915 6059 1238
Size: 117 62 59 2023 81 335 2144 1466
Fragments arranged by size:
2144 2023 1466 335 117 81 62 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4654 4654
Size: 6287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 625 1020 1374 1695 2522 2602 2719 3187 625
Size: 395 354 321 827 80 117 468 3725
Fragments arranged by size:
3725 827 468 395 354 321 117 80
Bpu10I CC'TnA_GC
Cuts at: 1235 1661 2313 2525 2983 1235
Size: 426 652 212 458 4539
Fragments arranged by size:
4539 652 458 426 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 3463 4342 4834 5132 1628
Size: 1835 879 492 298 2783
Fragments arranged by size:
2783 1835 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 145 856 2617 5991 145
Size: 711 1761 3374 441
Fragments arranged by size:
3374 1761 711 441
BsaAI yAC'GTr
Cuts at: 3279 3640 3279
Size: 361 5926
BsaBI GATnn'nnATC
Cuts at: 6 3801 5838 6
Size: 3795 2037 455
Fragments arranged by size:
3795 2037 455
BsaHI Gr'CG_yC
Cuts at: 200 497 781 1240 1406 1589 1684 2086 3718
Size: 297 284 459 166 183 95 402 1632
Cuts at: 3718 3966 4668 6164 200
Size: 248 702 1496 323
Fragments arranged by size:
1632 1496 702 459 402 323 297 284
248 183 166 95
BsaWI w'CCGG_w
Cuts at: 2099 3476 3997 4675 4949 5096 5507 2099
Size: 1377 521 678 274 147 411 2879
Fragments arranged by size:
2879 1377 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1048 1078 1317 1347 1623 1653 1048
Size: 30 239 30 276 30 5682
Fragments arranged by size:
5682 276 239 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1631 1728 2635 1631
Size: 97 907 5283
Fragments arranged by size:
5283 907 97
BseYI C'CCAG_C
Cuts at: 303 710 1773 1895 2035 2353 2517 3712 4470
Size: 407 1063 122 140 318 164 1195 758
Cuts at: 4470 5047 5543 303
Size: 577 496 1047
Fragments arranged by size:
1195 1063 1047 758 577 496 407 318
164 140 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 496 1123 1296 1459 1727 1771 2320 2707
Size: 75 627 173 163 268 44 549 387
Cuts at: 2707 3100 3109 421
Size: 393 9 3599
Fragments arranged by size:
3599 627 549 393 387 268 173 163
75 44 9
BsiEI CG_ry'CG
Cuts at: 104 138 579 1323 3829 3875 5083 104
Size: 34 441 744 2506 46 1208 1308
Fragments arranged by size:
2506 1308 1208 744 441 46 34
BsiHKAI G_wGCw'C
Cuts at: 1288 1754 2416 2531 2645 3301 4079 4269 5061
Size: 466 662 115 114 656 778 190 792
Cuts at: 5061 1288
Size: 2514
Fragments arranged by size:
2514 792 778 662 656 466 190 115
114
BsmI GAATG_Cn'
Cuts at: 3035 3035
Size: 6287
Bsp1286I G_dGCh'C
Cuts at: 134 1004 1288 1754 1939 1993 2012 2155 2188
Size: 870 284 466 185 54 19 143 33
Cuts at: 2188 2349 2362 2398 2416 2431 2458 2504 2531
Size: 161 13 36 18 15 27 46 27
Cuts at: 2531 2645 2746 3301 3912 4005 4079 4269 4331
Size: 114 101 555 611 93 74 190 62
Cuts at: 4331 5061 134
Size: 730 1360
Fragments arranged by size:
1360 870 730 611 555 466 284 190
185 161 143 114 101 93 74 62
54 46 36 33 27 27 19 18
15 13
BspEI T'CCGG_A
Cuts at: 5507 5507
Size: 6287
BspHI T'CATG_A
Cuts at: 5463 5463
Size: 6287
BspMI ACCTGCnnnn'nnnn_
Cuts at: 443 818 1388 1425 1475 1505 1709 1937 2057
Size: 375 570 37 50 30 204 228 120
Cuts at: 2057 2234 2916 3337 3551 3853 4234 443
Size: 177 682 421 214 302 381 2496
Fragments arranged by size:
2496 682 570 421 381 375 302 228
214 204 177 120 50 37 30
BsrBI CCG'CTC
Cuts at: 50 1665 1679 2408 3240 4579 4633 6117 6282
Size: 1615 14 729 832 1339 54 1484 165
Cuts at: 6282 50
Size: 55
Fragments arranged by size:
1615 1484 1339 832 729 165 55 54
14
BsrDI GCAATG_nn'
Cuts at: 2567 4199 2567
Size: 1632 4655
BsrFI r'CCGG_y
Cuts at: 574 1622 2195 2893 4285 4466 4675 574
Size: 1048 573 698 1392 181 209 2186
Fragments arranged by size:
2186 1392 1048 698 573 209 181
BsrGI T'GTAC_A
Cuts at: 683 2951 683
Size: 2268 4019
BssSI C'ACGA_G
Cuts at: 4558 4916 4558
Size: 358 5929
BstAPI GCAn_nnn'nTGC
Cuts at: 444 1095 1400 2903 2931 3087 3183 3230 3664
Size: 651 305 1503 28 156 96 47 434
Cuts at: 3664 444
Size: 3067
Fragments arranged by size:
3067 1503 651 434 305 156 96 47
28
BstBI TT'CG_AA
Cuts at: 110 4648 110
Size: 4538 1749
BstEII G'GTnAC_C
Cuts at: 1222 1222
Size: 6287
BstXI CCAn_nnnn'nTGG
Cuts at: 687 1536 687
Size: 849 5438
BstYI r'GATC_y
Cuts at: 1 2651 2885 3312 3779 3802 4137 4383 5395
Size: 2650 234 427 467 23 335 246 1012
Cuts at: 5395 5481 5493 5839 1
Size: 86 12 346 449
Fragments arranged by size:
2650 1012 467 449 427 346 335 246
234 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1368 2618 1368
Size: 1250 5037
BtgI C'CryG_G
Cuts at: 125 138 155 195 1317 1847 1905 2136 2297
Size: 13 17 40 1122 530 58 231 161
Cuts at: 2297 2390 125
Size: 93 4022
Fragments arranged by size:
4022 1122 530 231 161 93 58 40
17 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4237 4418 4581 4237
Size: 181 163 5943
Fragments arranged by size:
5943 181 163
BtsI GCAGTG_nn'
Cuts at: 1284 3047 3530 1284
Size: 1763 483 4041
Fragments arranged by size:
4041 1763 483
DraI TTT'AAA
Cuts at: 3286 3286
Size: 6287
DraIII CAC_nnn'GTG
Cuts at: 2493 3078 3221 2493
Size: 585 143 5559
Fragments arranged by size:
5559 585 143
DrdI GACnn_nn'nnGTC
Cuts at: 1195 1816 3993 4851 1195
Size: 621 2177 858 2631
Fragments arranged by size:
2631 2177 858 621
EaeI y'GGCC_r
Cuts at: 135 192 425 576 659 1320 1595 1666 2164
Size: 57 233 151 83 661 275 71 498
Cuts at: 2164 2193 2774 2843 3872 4046 4437 4464 5809
Size: 29 581 69 1029 174 391 27 1345
Cuts at: 5809 135
Size: 613
Fragments arranged by size:
1345 1029 661 613 581 498 391 275
233 174 151 83 71 69 57 29
27
EagI C'GGCC_G
Cuts at: 135 576 1320 3872 135
Size: 441 744 2552 2550
Fragments arranged by size:
2552 2550 744 441
EarI CTCTTCn'nnn_
Cuts at: 481 1179 2481 4310 4520 481
Size: 698 1302 1829 210 2248
Fragments arranged by size:
2248 1829 1302 698 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 745 1813 4495 4805 4951 6097 745
Size: 1068 2682 310 146 1146 935
Fragments arranged by size:
2682 1146 1068 935 310 146
EcoICRI GAG'CTC
Cuts at: 1286 2529 3299 1286
Size: 1243 770 4274
Fragments arranged by size:
4274 1243 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 625 1020 1374 1695 2522 2602 2719 2734
Size: 279 395 354 321 827 80 117 15
Cuts at: 2734 2857 3051 3187 4112 4544 5291 5559 346
Size: 123 194 136 925 432 747 268 1074
Fragments arranged by size:
1074 925 827 747 432 395 354 321
279 268 194 136 123 117 80 15
EcoNI CCTnn'n_nnAGG
Cuts at: 975 1730 2119 2537 2617 975
Size: 755 389 418 80 4645
Fragments arranged by size:
4645 755 418 389 80
EcoO109I rG'GnC_Cy
Cuts at: 183 420 807 1721 2128 2152 2175 2184 2185
Size: 237 387 914 407 24 23 9 1
Cuts at: 2185 2427 2428 2455 2501 2514 2604 2742 2743
Size: 242 1 27 46 13 90 138 1
Cuts at: 2743 2979 3153 3426 183
Size: 236 174 273 3044
Fragments arranged by size:
3044 914 407 387 273 242 237 236
174 138 90 46 27 24 23 13
9 1 1 1
EcoRI G'AATT_C
Cuts at: 3291 3291
Size: 6287
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 801 2205 4306 801
Size: 1404 2101 2782
Fragments arranged by size:
2782 2101 1404
FseI GG_CCGG'CC
Cuts at: 1626 1626
Size: 6287
FspI TGC'GCA
Cuts at: 448 2935 4068 448
Size: 2487 1133 2667
Fragments arranged by size:
2667 2487 1133
FspAI rTGC'GCAy
Cuts at: 448 448
Size: 6287
HaeII r_GCGC'y
Cuts at: 203 500 784 1243 1409 1796 2726 3003 3256
Size: 297 284 459 166 387 930 277 253
Cuts at: 3256 3721 3969 4991 5970 203
Size: 465 248 1022 979 520
Fragments arranged by size:
1022 979 930 520 465 459 387 297
284 277 253 248 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1317 1349 2076 2108 2839 2871 4129 4159 4161
Size: 32 727 32 731 32 1258 30 2
Cuts at: 4161 4191 1317
Size: 30 3413
Fragments arranged by size:
3413 1258 731 727 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3326 3326
Size: 6287
KasI G'GCGC_C
Cuts at: 199 496 780 1239 1405 3717 3965 199
Size: 297 284 459 166 2312 248 2521
Fragments arranged by size:
2521 2312 459 297 284 248 166
KpnI G_GTAC'C
Cuts at: 639 3307 639
Size: 2668 3619
MluI A'CGCG_T
Cuts at: 5720 5720
Size: 6287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 590 1629 4958 5142 590
Size: 1039 3329 184 1735
Fragments arranged by size:
3329 1735 1039 184
MscI TGG'CCA
Cuts at: 1597 4048 1597
Size: 2451 3836
MslI CAynn'nnrTG
Cuts at: 685 1435 2440 4276 4403 685
Size: 750 1005 1836 127 2569
Fragments arranged by size:
2569 1836 1005 750 127
MspA1I CmG'CkG
Cuts at: 140 876 885 1095 1129 2317 2452 2757 2823
Size: 736 9 210 34 1188 135 305 66
Cuts at: 2823 3021 3398 3712 4072 5085 5330 140
Size: 198 377 314 360 1013 245 1097
Fragments arranged by size:
1188 1097 1013 736 377 360 314 305
245 210 198 135 66 34 9
NaeI GCC'GGC
Cuts at: 576 1624 2895 4468 576
Size: 1048 1271 1573 2395
Fragments arranged by size:
2395 1573 1271 1048
NarI GG'CG_CC
Cuts at: 200 497 781 1240 1406 3718 3966 200
Size: 297 284 459 166 2312 248 2521
Fragments arranged by size:
2521 2312 459 297 284 248 166
NcoI C'CATG_G
Cuts at: 125 2136 2297 125
Size: 2011 161 4115
Fragments arranged by size:
4115 2011 161
NgoMIV G'CCGG_C
Cuts at: 574 1622 2893 4466 574
Size: 1048 1271 1573 2395
Fragments arranged by size:
2395 1573 1271 1048
NspI r_CATG'y
Cuts at: 3186 3340 3840 4747 3186
Size: 154 500 907 4726
Fragments arranged by size:
4726 907 500 154
PasI CC'CwG_GG
Cuts at: 1077 1536 1857 2424 1077
Size: 459 321 567 4940
Fragments arranged by size:
4940 567 459 321
PciI A'CATG_T
Cuts at: 4743 4743
Size: 6287
PflMI CCAn_nnn'nTGG
Cuts at: 375 375
Size: 6287
PfoI T'CCnGG_A
Cuts at: 368 2538 2696 3008 3026 3164 368
Size: 2170 158 312 18 138 3491
Fragments arranged by size:
3491 2170 312 158 138 18
PmeI GTTT'AAAC
Cuts at: 3286 3286
Size: 6287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5426 5458 5426
Size: 32 6255
PpuMI rG'GwC_Cy
Cuts at: 183 420 807 1721 2128 2175 183
Size: 237 387 914 407 47 4295
Fragments arranged by size:
4295 914 407 387 237 47
PshAI GACnn'nnGTC
Cuts at: 841 841
Size: 6287
PspOMI G'GGCC_C
Cuts at: 2151 2184 2427 2454 2500 2742 2151
Size: 33 243 27 46 242 5696
Fragments arranged by size:
5696 243 242 46 33 27
PstI C_TGCA'G
Cuts at: 485 1100 1351 1472 1916 1964 2288 2509 2554
Size: 615 251 121 444 48 324 221 45
Cuts at: 2554 2762 2999 3179 3334 4019 485
Size: 208 237 180 155 685 2753
Fragments arranged by size:
2753 685 615 444 324 251 237 221
208 180 155 121 48 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6287
PvuII CAG'CTG
Cuts at: 885 1095 2317 2757 2823 3712 4072 885
Size: 210 1222 440 66 889 360 3100
Fragments arranged by size:
3100 1222 889 440 360 210 66
RsrII CG'GwC_CG
Cuts at: 4482 4482
Size: 6287
SacI G_AGCT'C
Cuts at: 1288 2531 3301 1288
Size: 1243 770 4274
Fragments arranged by size:
4274 1243 770
SacII CC_GC'GG
Cuts at: 141 141
Size: 6287
SalI G'TCGA_C
Cuts at: 3324 3324
Size: 6287
SanDI GG'GwC_CC
Cuts at: 2128 2128
Size: 6287
SapI GCTCTTCn'nnn_
Cuts at: 481 2481 4310 4520 481
Size: 2000 1829 210 2248
Fragments arranged by size:
2248 2000 1829 210
SbfI CC_TGCA'GG
Cuts at: 2288 3334 2288
Size: 1046 5241
ScaI AGT'ACT
Cuts at: 406 5804 406
Size: 5398 889
SexAI A'CCwGG_T
Cuts at: 392 1226 2489 392
Size: 834 1263 4190
Fragments arranged by size:
4190 1263 834
SfcI C'TryA_G
Cuts at: 33 481 1096 1347 1468 1912 1960 2284 2505
Size: 448 615 251 121 444 48 324 221
Cuts at: 2505 2550 2758 2995 3175 3330 4015 5008 5199
Size: 45 208 237 180 155 685 993 191
Cuts at: 5199 33
Size: 1121
Fragments arranged by size:
1121 993 685 615 448 444 324 251
237 221 208 191 180 155 121 48
45
SfiI GGCCn_nnn'nGGCC
Cuts at: 1379 2192 1379
Size: 813 5474
SfoI GGC'GCC
Cuts at: 201 498 782 1241 1407 3719 3967 201
Size: 297 284 459 166 2312 248 2521
Fragments arranged by size:
2521 2312 459 297 284 248 166
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6287
SmaI CCC'GGG
Cuts at: 859 1150 2267 3309 859
Size: 291 1117 1042 3837
Fragments arranged by size:
3837 1117 1042 291
SmlI C'TyrA_G
Cuts at: 1643 2233 3442 4357 4849 5111 1643
Size: 590 1209 915 492 262 2819
Fragments arranged by size:
2819 1209 915 590 492 262
SnaBI TAC'GTA
Cuts at: 3279 3279
Size: 6287
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6287
SphI G_CATG'C
Cuts at: 3340 3840 3340
Size: 500 5787
StyI C'CwwG_G
Cuts at: 125 2000 2136 2180 2297 3421 3775 125
Size: 1875 136 44 117 1124 354 2637
Fragments arranged by size:
2637 1875 1124 354 136 117 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 963 1487 1491 1495 1603 1956 2354 3870 4003
Size: 524 4 4 108 353 398 1516 133
Cuts at: 4003 4462 4671 963
Size: 459 209 2579
Fragments arranged by size:
2579 1516 524 459 398 353 209 133
108 4 4
TatI w'GTAC_w
Cuts at: 404 683 2951 5802 404
Size: 279 2268 2851 889
Fragments arranged by size:
2851 2268 889 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1048 1080 1623 1655 5804 5836 1048
Size: 32 543 32 4149 32 1499
Fragments arranged by size:
4149 1499 543 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1195 4084 1195
Size: 2889 3398
XbaI T'CTAG_A
Cuts at: 3318 3318
Size: 6287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1056 1579 1854 2494 3782 1056
Size: 523 275 640 1288 3561
Fragments arranged by size:
3561 1288 640 523 275
XmaI C'CCGG_G
Cuts at: 857 1148 2265 3307 857
Size: 291 1117 1042 3837
Fragments arranged by size:
3837 1117 1042 291
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AhdI AscI AvrII BaeI BfrBI BmtI BsiWI
BsmBI BssHII BstZ17I ClaI CspCI EcoRV HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI PsrI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI