Restriction map for
pF1KSDA0719
(Circular) (Six-base) MAPSORT of: pF1KSDA0719.seq Check: 751 from: 1 to: 4963
pF1KSDA0719 4963 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1999 1999
Size: 4963
Acc65I G'GTAC_C
Cuts at: 1977 1977
Size: 4963
AclI AA'CG_TT
Cuts at: 4803 4803
Size: 4963
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 443 891 1115 1672 2786 3218 3967 4235
Size: 67 448 224 557 1114 432 749 268
Cuts at: 4235 376
Size: 1104
Fragments arranged by size:
1114 1104 749 557 448 432 268 224
67
AflIII A'CryG_T
Cuts at: 1823 2313 3419 4396 1823
Size: 490 1106 977 2390
Fragments arranged by size:
2390 1106 977 490
AgeI A'CCGG_T
Cuts at: 3351 3351
Size: 4963
AleI CACnn'nnGTG
Cuts at: 760 760
Size: 4963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1273 1273
Size: 4963
AlwNI CAG_nnn'CTG
Cuts at: 169 2266 3835 169
Size: 2097 1569 1297
Fragments arranged by size:
2097 1569 1297
ApaI G_GGCC'C
Cuts at: 217 217
Size: 4963
ApaLI G'TGCA_C
Cuts at: 3733 3733
Size: 4963
ApoI r'AATT_y
Cuts at: 1008 1537 1965 4316 4572 1008
Size: 529 428 2351 256 1399
Fragments arranged by size:
2351 1399 529 428 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4963
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4963
AvaI C'yCGr_G
Cuts at: 216 405 1981 216
Size: 189 1576 3198
Fragments arranged by size:
3198 1576 189
BamHI G'GATC_C
Cuts at: 1986 1986
Size: 4963
BanI G'GyrC_C
Cuts at: 264 286 317 1977 2391 2639 2674 264
Size: 22 31 1660 414 248 35 2553
Fragments arranged by size:
2553 1660 414 248 35 31 22
BanII G_rGCy'C
Cuts at: 181 217 468 1975 3005 181
Size: 36 251 1507 1030 2139
Fragments arranged by size:
2139 1507 1030 251 36
BbeI G_GCGC'C
Cuts at: 321 2395 2643 321
Size: 2074 248 2641
Fragments arranged by size:
2641 2074 248
BbsI GAAGACnn'nnnn_
Cuts at: 382 382
Size: 4963
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1543 1577 1543
Size: 34 4929
BciVI GTATCCnnnnn_n'
Cuts at: 1009 2859 3622 4963 1009
Size: 1850 763 1341 1009
Fragments arranged by size:
1850 1341 1009 763
BclI T'GATC_A
Cuts at: 1368 2481 1368
Size: 1113 3850
BglI GCCn_nnn'nGGC
Cuts at: 240 400 240
Size: 160 4803
BglII A'GATC_T
Cuts at: 1701 2476 4515 1701
Size: 775 2039 2149
Fragments arranged by size:
2149 2039 775
BlpI GC'TnA_GC
Cuts at: 2073 2073
Size: 4963
Bme1580I G_kGCm'C
Cuts at: 217 223 267 559 2586 2679 3737 217
Size: 6 44 292 2027 93 1058 1443
Fragments arranged by size:
2027 1443 1058 292 93 44 6
BmgBI CAC'GTC
Cuts at: 703 703
Size: 4963
BmrI ACTGGGnnnn_n'
Cuts at: 2173 2254 2589 4735 2173
Size: 81 335 2146 2401
Fragments arranged by size:
2401 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3328 3328
Size: 4963
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1271 2137 3016 3510 3808 1271
Size: 866 879 494 298 2426
Fragments arranged by size:
2426 879 866 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4667 4667
Size: 4963
BsaAI yAC'GTr
Cuts at: 1953 2314 1953
Size: 361 4602
BsaBI GATnn'nnATC
Cuts at: 6 1334 2475 4514 6
Size: 1328 1141 2039 455
Fragments arranged by size:
2039 1328 1141 455
BsaHI Gr'CG_yC
Cuts at: 318 344 2392 2640 3344 4840 318
Size: 26 2048 248 704 1496 441
Fragments arranged by size:
2048 1496 704 441 248 26
BsaWI w'CCGG_w
Cuts at: 2150 2671 3351 3625 3772 4183 2150
Size: 521 680 274 147 411 2930
Fragments arranged by size:
2930 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 276 935 2386 3144 3723 4219 276
Size: 659 1451 758 579 496 1020
Fragments arranged by size:
1451 1020 758 659 579 496
BsiEI CG_ry'CG
Cuts at: 104 260 2503 2549 3759 104
Size: 156 2243 46 1210 1308
Fragments arranged by size:
2243 1308 1210 156 46
BsiHKAI G_wGCw'C
Cuts at: 181 438 1975 2753 2943 3737 181
Size: 257 1537 778 190 794 1407
Fragments arranged by size:
1537 1407 794 778 257 190
BsmI GAATG_Cn'
Cuts at: 1078 1329 1078
Size: 251 4712
Bsp1286I G_dGCh'C
Cuts at: 181 217 223 267 438 468 559 1975 2586
Size: 36 6 44 171 30 91 1416 611
Cuts at: 2586 2679 2753 2943 3005 3737 181
Size: 93 74 190 62 732 1407
Fragments arranged by size:
1416 1407 732 611 190 171 93 91
74 62 44 36 30 6
BspEI T'CCGG_A
Cuts at: 4183 4183
Size: 4963
BspHI T'CATG_A
Cuts at: 4139 4139
Size: 4963
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1151 2011 2225 2527 2908 1151
Size: 860 214 302 381 3206
Fragments arranged by size:
3206 860 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3253 3307 4793 4958 50
Size: 3203 54 1486 165 55
Fragments arranged by size:
3203 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1117 1161 2873 1117
Size: 44 1712 3207
Fragments arranged by size:
3207 1712 44
BsrFI r'CCGG_y
Cuts at: 2959 3140 3351 2959
Size: 181 211 4571
Fragments arranged by size:
4571 211 181
BsrGI T'GTAC_A
Cuts at: 652 1253 652
Size: 601 4362
BssSI C'ACGA_G
Cuts at: 3232 3592 3232
Size: 360 4603
BstAPI GCAn_nnn'nTGC
Cuts at: 1275 2338 1275
Size: 1063 3900
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4963
BstYI r'GATC_y
Cuts at: 1 1701 1986 2453 2476 2811 3057 4071 4157
Size: 1700 285 467 23 335 246 1014 86
Cuts at: 4157 4169 4515 1
Size: 12 346 449
Fragments arranged by size:
1700 1014 467 449 346 335 285 246
86 23 12
BtgI C'CryG_G
Cuts at: 125 1775 125
Size: 1650 3313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 252 1904 2911 3092 3255 252
Size: 1652 1007 181 163 1960
Fragments arranged by size:
1960 1652 1007 181 163
BtsI GCAGTG_nn'
Cuts at: 755 1391 2204 755
Size: 636 813 3514
Fragments arranged by size:
3514 813 636
ClaI AT'CG_AT
Cuts at: 1313 1313
Size: 4963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1672 1707 1672
Size: 35 4928
DraI TTT'AAA
Cuts at: 501 1960 501
Size: 1459 3504
DrdI GACnn_nn'nnGTC
Cuts at: 2667 3527 2667
Size: 860 4103
EaeI y'GGCC_r
Cuts at: 128 1847 2546 2720 3111 3138 4485 128
Size: 1719 699 174 391 27 1347 606
Fragments arranged by size:
1719 1347 699 606 391 174 27
EagI C'GGCC_G
Cuts at: 2546 2546
Size: 4963
EarI CTCTTCn'nnn_
Cuts at: 1494 1517 2984 3194 1494
Size: 23 1467 210 3263
Fragments arranged by size:
3263 1467 210 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3169 3481 3627 4773 3169
Size: 312 146 1146 3359
Fragments arranged by size:
3359 1146 312 146
EcoICRI GAG'CTC
Cuts at: 179 1973 179
Size: 1794 3169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 443 891 1115 1672 2786 3218 3967 4235
Size: 67 448 224 557 1114 432 749 268
Cuts at: 4235 376
Size: 1104
Fragments arranged by size:
1114 1104 749 557 448 432 268 224
67
EcoO109I rG'GnC_Cy
Cuts at: 427 2100 427
Size: 1673 3290
EcoRI G'AATT_C
Cuts at: 1965 1965
Size: 4963
EcoRV GAT'ATC
Cuts at: 927 927
Size: 4963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 2980 1696
Size: 1284 3679
FspI TGC'GCA
Cuts at: 2742 2742
Size: 4963
HaeII r_GCGC'y
Cuts at: 321 2395 2643 3667 4646 321
Size: 2074 248 1024 979 638
Fragments arranged by size:
2074 1024 979 638 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 436 468 867 899 1857 1889 2803 2833 2835
Size: 32 399 32 958 32 914 30 2
Cuts at: 2835 2865 436
Size: 30 2534
Fragments arranged by size:
2534 958 914 399 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 576 2000 576
Size: 1424 3539
HindIII A'AGCT_T
Cuts at: 1216 1216
Size: 4963
KasI G'GCGC_C
Cuts at: 317 2391 2639 317
Size: 2074 248 2641
Fragments arranged by size:
2641 2074 248
KpnI G_GTAC'C
Cuts at: 1981 1981
Size: 4963
MluI A'CGCG_T
Cuts at: 4396 4396
Size: 4963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3634 3818 3634
Size: 184 4779
MscI TGG'CCA
Cuts at: 1849 2722 1849
Size: 873 4090
MslI CAynn'nnrTG
Cuts at: 760 1255 1446 1822 2950 3077 760
Size: 495 191 376 1128 127 2646
Fragments arranged by size:
2646 1128 495 376 191 127
MspA1I CmG'CkG
Cuts at: 154 169 246 350 413 1353 2072 2386 2746
Size: 15 77 104 63 940 719 314 360
Cuts at: 2746 3761 4006 154
Size: 1015 245 1111
Fragments arranged by size:
1111 1015 940 719 360 314 245 104
77 63 15
NaeI GCC'GGC
Cuts at: 3142 3142
Size: 4963
NarI GG'CG_CC
Cuts at: 318 2392 2640 318
Size: 2074 248 2641
Fragments arranged by size:
2641 2074 248
NcoI C'CATG_G
Cuts at: 125 1775 125
Size: 1650 3313
NdeI CA'TA_TG
Cuts at: 1662 1662
Size: 4963
NgoMIV G'CCGG_C
Cuts at: 3140 3140
Size: 4963
NruI TCG'CGA
Cuts at: 3324 3324
Size: 4963
NspI r_CATG'y
Cuts at: 801 1254 1260 1827 2014 2514 3423 801
Size: 453 6 567 187 500 909 2341
Fragments arranged by size:
2341 909 567 500 453 187 6
PciI A'CATG_T
Cuts at: 1823 3419 1823
Size: 1596 3367
PfoI T'CCnGG_A
Cuts at: 181 181
Size: 4963
PmeI GTTT'AAAC
Cuts at: 1960 1960
Size: 4963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4102 4134 4102
Size: 32 4931
PpuMI rG'GwC_Cy
Cuts at: 427 427
Size: 4963
PshAI GACnn'nnGTC
Cuts at: 1422 1422
Size: 4963
PspOMI G'GGCC_C
Cuts at: 213 213
Size: 4963
PstI C_TGCA'G
Cuts at: 2008 2693 2008
Size: 685 4278
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4963
PvuII CAG'CTG
Cuts at: 246 1353 2386 2746 246
Size: 1107 1033 360 2463
Fragments arranged by size:
2463 1107 1033 360
RsrII CG'GwC_CG
Cuts at: 3156 3156
Size: 4963
SacI G_AGCT'C
Cuts at: 181 1975 181
Size: 1794 3169
SalI G'TCGA_C
Cuts at: 1998 1998
Size: 4963
SapI GCTCTTCn'nnn_
Cuts at: 2984 3194 2984
Size: 210 4753
SbfI CC_TGCA'GG
Cuts at: 2008 2008
Size: 4963
ScaI AGT'ACT
Cuts at: 4480 4480
Size: 4963
SfcI C'TryA_G
Cuts at: 33 560 2004 2689 3684 3875 33
Size: 527 1444 685 995 191 1121
Fragments arranged by size:
1444 1121 995 685 527 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 400 400
Size: 4963
SfoI GGC'GCC
Cuts at: 319 2393 2641 319
Size: 2074 248 2641
Fragments arranged by size:
2641 2074 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4963
SmaI CCC'GGG
Cuts at: 218 407 1983 218
Size: 189 1576 3198
Fragments arranged by size:
3198 1576 189
SmlI C'TyrA_G
Cuts at: 1286 2116 3031 3525 3787 1286
Size: 830 915 494 262 2462
Fragments arranged by size:
2462 915 830 494 262
SnaBI TAC'GTA
Cuts at: 1953 1953
Size: 4963
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4963
SphI G_CATG'C
Cuts at: 801 2014 2514 801
Size: 1213 500 3250
Fragments arranged by size:
3250 1213 500
SrfI GCCC'GGGC
Cuts at: 218 218
Size: 4963
SspI AAT'ATT
Cuts at: 496 496
Size: 4963
StyI C'CwwG_G
Cuts at: 125 828 1543 1775 2095 2449 125
Size: 703 715 232 320 354 2639
Fragments arranged by size:
2639 715 703 354 320 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2544 2677 3136 3347 2544
Size: 133 459 211 4160
Fragments arranged by size:
4160 459 211 133
TatI w'GTAC_w
Cuts at: 652 1253 1791 4478 652
Size: 601 538 2687 1137
Fragments arranged by size:
2687 1137 601 538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4480 4512 4480
Size: 32 4931
Tth111I GACn'n_nGTC
Cuts at: 2758 2758
Size: 4963
XbaI T'CTAG_A
Cuts at: 1992 1992
Size: 4963
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1035 1857 2456 1035
Size: 822 599 3542
Fragments arranged by size:
3542 822 599
XmaI C'CCGG_G
Cuts at: 216 405 1981 216
Size: 189 1576 3198
Fragments arranged by size:
3198 1576 189
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NspI
PciI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BmtI BpmI Bpu10I BsaXI BseRI
BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
DraIII EcoNI FseI FspAI HpaI MfeI NheI NotI
NsiI PacI PasI PflMI PmlI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI StuI SwaI XhoI XmnI
ZraI