Restriction map for
pF1KSDA0717
(Circular) (Six-base) MAPSORT of: pF1KSDA0717.seq Check: 2996 from: 1 to: 5320
pF1KSDA0717 5320 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 773 773
Size: 5320
AatII G_ACGT'C
Cuts at: 722 929 1961 722
Size: 207 1032 4081
Fragments arranged by size:
4081 1032 207
AccI GT'mk_AC
Cuts at: 2306 2356 2306
Size: 50 5270
Acc65I G'GTAC_C
Cuts at: 491 1256 2135 2334 491
Size: 765 879 199 3477
Fragments arranged by size:
3477 879 765 199
AclI AA'CG_TT
Cuts at: 5160 5160
Size: 5320
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2161 3143 3575 4324 4592 2161
Size: 982 432 749 268 2889
Fragments arranged by size:
2889 982 749 432 268
AflIII A'CryG_T
Cuts at: 1163 2048 2670 3776 4753 1163
Size: 885 622 1106 977 1730
Fragments arranged by size:
1730 1106 977 885 622
AgeI A'CCGG_T
Cuts at: 3708 3708
Size: 5320
AleI CACnn'nnGTG
Cuts at: 271 2130 271
Size: 1859 3461
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2230 2262 2230
Size: 32 5288
AlwNI CAG_nnn'CTG
Cuts at: 261 2623 4192 261
Size: 2362 1569 1389
Fragments arranged by size:
2362 1569 1389
ApaI G_GGCC'C
Cuts at: 1048 1400 1048
Size: 352 4968
ApaLI G'TGCA_C
Cuts at: 4090 4090
Size: 5320
ApoI r'AATT_y
Cuts at: 2322 4673 4929 2322
Size: 2351 256 2713
Fragments arranged by size:
2713 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5320
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5320
AvaI C'yCGr_G
Cuts at: 332 518 1489 1739 2066 2338 332
Size: 186 971 250 327 272 3314
Fragments arranged by size:
3314 971 327 272 250 186
BamHI G'GATC_C
Cuts at: 2343 2343
Size: 5320
BanI G'GyrC_C
Cuts at: 491 816 858 1063 1256 1615 1651 2135 2334
Size: 325 42 205 193 359 36 484 199
Cuts at: 2334 2748 2996 3031 491
Size: 414 248 35 2780
Fragments arranged by size:
2780 484 414 359 325 248 205 199
193 42 36 35
BanII G_rGCy'C
Cuts at: 1048 1195 1400 1735 2332 3362 1048
Size: 147 205 335 597 1030 3006
Fragments arranged by size:
3006 1030 597 335 205 147
BbeI G_GCGC'C
Cuts at: 2752 3000 2752
Size: 248 5072
BbsI GAAGACnn'nnnn_
Cuts at: 1277 2249 1277
Size: 972 4348
BciVI GTATCCnnnnn_n'
Cuts at: 3216 3979 5320 3216
Size: 763 1341 3216
Fragments arranged by size:
3216 1341 763
BclI T'GATC_A
Cuts at: 1095 2838 1095
Size: 1743 3577
BglI GCCn_nnn'nGGC
Cuts at: 2220 2220
Size: 5320
BglII A'GATC_T
Cuts at: 421 971 2833 4872 421
Size: 550 1862 2039 869
Fragments arranged by size:
2039 1862 869 550
BlpI GC'TnA_GC
Cuts at: 1481 2430 1481
Size: 949 4371
Bme1580I G_kGCm'C
Cuts at: 225 278 863 892 1048 1066 1400 2169 2943
Size: 53 585 29 156 18 334 769 774
Cuts at: 2943 3036 4094 225
Size: 93 1058 1451
Fragments arranged by size:
1451 1058 774 769 585 334 156 93
53 29 18
BmgBI CAC'GTC
Cuts at: 339 1164 1575 339
Size: 825 411 4084
Fragments arranged by size:
4084 825 411
BmrI ACTGGGnnnn_n'
Cuts at: 243 696 2530 2611 2946 5092 243
Size: 453 1834 81 335 2146 471
Fragments arranged by size:
2146 1834 471 453 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 3685 763
Size: 2922 2398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 858 925 1311 1803 592
Size: 266 67 386 492 4109
Fragments arranged by size:
4109 492 386 266 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2188 2494 3373 3867 4165 1399
Size: 789 306 879 494 298 2554
Fragments arranged by size:
2554 879 789 494 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 157 380 679 5024 157
Size: 223 299 4345 453
Fragments arranged by size:
4345 453 299 223
BsaAI yAC'GTr
Cuts at: 2310 2671 2310
Size: 361 4959
BsaBI GATnn'nnATC
Cuts at: 6 2832 4871 6
Size: 2826 2039 455
Fragments arranged by size:
2826 2039 455
BsaHI Gr'CG_yC
Cuts at: 719 926 1958 2749 2997 3701 5197 719
Size: 207 1032 791 248 704 1496 842
Fragments arranged by size:
1496 1032 842 791 704 248 207
BsaWI w'CCGG_w
Cuts at: 1195 2507 3028 3708 3982 4129 4540 1195
Size: 1312 521 680 274 147 411 1975
Fragments arranged by size:
1975 1312 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 856 886 1735 1765 856
Size: 30 849 30 4411
Fragments arranged by size:
4411 849 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 898 978 981 2262 2265 2283 898
Size: 80 3 1281 3 18 3935
Fragments arranged by size:
3935 1281 80 18 3 3
BseYI C'CCAG_C
Cuts at: 666 1038 1186 2743 3501 4080 4576 666
Size: 372 148 1557 758 579 496 1410
Fragments arranged by size:
1557 1410 758 579 496 372 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 817 2016 728
Size: 89 1199 4032
Fragments arranged by size:
4032 1199 89
BsiEI CG_ry'CG
Cuts at: 104 2860 2906 4116 104
Size: 2756 46 1210 1308
Fragments arranged by size:
2756 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 525 1735 2332 3110 3300 4094 525
Size: 1210 597 778 190 794 1751
Fragments arranged by size:
1751 1210 794 778 597 190
BsmBI CGTCTCn'nnnn_
Cuts at: 329 363 329
Size: 34 5286
Bsp1286I G_dGCh'C
Cuts at: 225 278 525 863 892 1048 1066 1195 1400
Size: 53 247 338 29 156 18 129 205
Cuts at: 1400 1735 2169 2332 2943 3036 3110 3300 3362
Size: 335 434 163 611 93 74 190 62
Cuts at: 3362 4094 225
Size: 732 1451
Fragments arranged by size:
1451 732 611 434 338 335 247 205
190 163 156 129 93 74 62 53
29 18
BspEI T'CCGG_A
Cuts at: 4540 4540
Size: 5320
BspHI T'CATG_A
Cuts at: 1544 4496 1544
Size: 2952 2368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 563 773 2368 2582 2884 3265 563
Size: 210 1595 214 302 381 2618
Fragments arranged by size:
2618 1595 381 302 214 210
BsrBI CCG'CTC
Cuts at: 50 915 948 3610 3664 5150 5315 50
Size: 865 33 2662 54 1486 165 55
Fragments arranged by size:
2662 1486 865 165 55 54 33
BsrDI GCAATG_nn'
Cuts at: 240 449 798 1466 1475 3230 240
Size: 209 349 668 9 1755 2330
Fragments arranged by size:
2330 1755 668 349 209 9
BsrFI r'CCGG_y
Cuts at: 1804 3316 3497 3708 1804
Size: 1512 181 211 3416
Fragments arranged by size:
3416 1512 211 181
BsrGI T'GTAC_A
Cuts at: 1424 1424
Size: 5320
BssSI C'ACGA_G
Cuts at: 3589 3949 3589
Size: 360 4960
BstAPI GCAn_nnn'nTGC
Cuts at: 1772 2695 1772
Size: 923 4397
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5320
BstXI CCAn_nnnn'nTGG
Cuts at: 666 696 1126 2132 666
Size: 30 430 1006 3854
Fragments arranged by size:
3854 1006 430 30
BstYI r'GATC_y
Cuts at: 1 421 971 1232 1332 2343 2810 2833 3168
Size: 420 550 261 100 1011 467 23 335
Cuts at: 3168 3414 4428 4514 4526 4872 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1014 1011 550 467 449 420 346 335
261 246 100 86 23 12
BtgI C'CryG_G
Cuts at: 125 197 1125 125
Size: 72 928 4320
Fragments arranged by size:
4320 928 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3268 3449 3612 3268
Size: 181 163 4976
Fragments arranged by size:
4976 181 163
BtsI GCAGTG_nn'
Cuts at: 2561 2561
Size: 5320
ClaI AT'CG_AT
Cuts at: 1949 1949
Size: 5320
DraI TTT'AAA
Cuts at: 2317 2317
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 3024 3884 3024
Size: 860 4460
EaeI y'GGCC_r
Cuts at: 263 656 1517 2006 2122 2903 3077 3468 3495
Size: 393 861 489 116 781 174 391 27
Cuts at: 3495 4842 263
Size: 1347 741
Fragments arranged by size:
1347 861 781 741 489 393 391 174
116 27
EagI C'GGCC_G
Cuts at: 2903 2903
Size: 5320
EarI CTCTTCn'nnn_
Cuts at: 1313 2236 3341 3551 1313
Size: 923 1105 210 3082
Fragments arranged by size:
3082 1105 923 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1567 3526 3838 3984 5130 1567
Size: 1959 312 146 1146 1757
Fragments arranged by size:
1959 1757 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1733 2330 1733
Size: 597 4723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 858 925 1311 1803 2161 3143 3575 4324
Size: 266 67 386 492 358 982 432 749
Cuts at: 4324 4592 592
Size: 268 1320
Fragments arranged by size:
1320 982 749 492 432 386 358 268
266 67
EcoNI CCTnn'n_nnAGG
Cuts at: 603 904 1027 2143 603
Size: 301 123 1116 3780
Fragments arranged by size:
3780 1116 301 123
EcoO109I rG'GnC_Cy
Cuts at: 909 1044 1045 1396 1397 2457 909
Size: 135 1 351 1 1060 3772
Fragments arranged by size:
3772 1060 351 135 1 1
EcoRI G'AATT_C
Cuts at: 2322 2322
Size: 5320
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 738 1620 3337 738
Size: 882 1717 2721
Fragments arranged by size:
2721 1717 882
FspI TGC'GCA
Cuts at: 955 1507 3099 955
Size: 552 1592 3176
Fragments arranged by size:
3176 1592 552
FspAI rTGC'GCAy
Cuts at: 955 955
Size: 5320
HaeII r_GCGC'y
Cuts at: 1664 2752 3000 4024 5003 1664
Size: 1088 248 1024 979 1981
Fragments arranged by size:
1981 1088 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 512 544 856 888 3160 3190 3192 3222 512
Size: 32 312 32 2272 30 2 30 2610
Fragments arranged by size:
2610 2272 312 32 32 30 30 2
HincII GTy'rAC
Cuts at: 582 2357 582
Size: 1775 3545
KasI G'GCGC_C
Cuts at: 2748 2996 2748
Size: 248 5072
KpnI G_GTAC'C
Cuts at: 495 1260 2139 2338 495
Size: 765 879 199 3477
Fragments arranged by size:
3477 879 765 199
MluI A'CGCG_T
Cuts at: 4753 4753
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 530 3991 4175 530
Size: 3461 184 1675
Fragments arranged by size:
3461 1675 184
MscI TGG'CCA
Cuts at: 265 658 1519 2124 3079 265
Size: 393 861 605 955 2506
Fragments arranged by size:
2506 955 861 605 393
MslI CAynn'nnrTG
Cuts at: 271 535 856 934 1124 1513 1807 2130 3307
Size: 264 321 78 190 389 294 323 1177
Cuts at: 3307 3434 271
Size: 127 2157
Fragments arranged by size:
2157 1177 389 323 321 294 264 190
127 78
MspA1I CmG'CkG
Cuts at: 258 801 1688 2004 2163 2429 2743 3103 4118
Size: 543 887 316 159 266 314 360 1015
Cuts at: 4118 4363 258
Size: 245 1215
Fragments arranged by size:
1215 1015 887 543 360 316 314 266
245 159
NaeI GCC'GGC
Cuts at: 3499 3499
Size: 5320
NarI GG'CG_CC
Cuts at: 2749 2997 2749
Size: 248 5072
NcoI C'CATG_G
Cuts at: 125 1125 125
Size: 1000 4320
NgoMIV G'CCGG_C
Cuts at: 3497 3497
Size: 5320
NruI TCG'CGA
Cuts at: 3681 3681
Size: 5320
NspI r_CATG'y
Cuts at: 274 1779 1812 2371 2871 3780 274
Size: 1505 33 559 500 909 1814
Fragments arranged by size:
1814 1505 909 559 500 33
PciI A'CATG_T
Cuts at: 3776 3776
Size: 5320
PflMI CCAn_nnn'nTGG
Cuts at: 261 284 903 1938 261
Size: 23 619 1035 3643
Fragments arranged by size:
3643 1035 619 23
PfoI T'CCnGG_A
Cuts at: 902 1288 1313 1640 1970 902
Size: 386 25 327 330 4252
Fragments arranged by size:
4252 386 330 327 25
PmeI GTTT'AAAC
Cuts at: 2317 2317
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4459 4491 4459
Size: 32 5288
PpuMI rG'GwC_Cy
Cuts at: 909 909
Size: 5320
PshAI GACnn'nnGTC
Cuts at: 2015 2015
Size: 5320
PspOMI G'GGCC_C
Cuts at: 1044 1396 1044
Size: 352 4968
PspXI vC'TCGA_Gb
Cuts at: 1489 1489
Size: 5320
PstI C_TGCA'G
Cuts at: 770 815 944 2365 3050 770
Size: 45 129 1421 685 3040
Fragments arranged by size:
3040 1421 685 129 45
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5320
PvuII CAG'CTG
Cuts at: 258 1688 2004 2743 3103 258
Size: 1430 316 739 360 2475
Fragments arranged by size:
2475 1430 739 360 316
RsrII CG'GwC_CG
Cuts at: 3513 3513
Size: 5320
SacI G_AGCT'C
Cuts at: 1735 2332 1735
Size: 597 4723
SalI G'TCGA_C
Cuts at: 2355 2355
Size: 5320
SapI GCTCTTCn'nnn_
Cuts at: 2236 3341 3551 2236
Size: 1105 210 4005
Fragments arranged by size:
4005 1105 210
SbfI CC_TGCA'GG
Cuts at: 2365 2365
Size: 5320
ScaI AGT'ACT
Cuts at: 4837 4837
Size: 5320
SexAI A'CCwGG_T
Cuts at: 1880 1880
Size: 5320
SfcI C'TryA_G
Cuts at: 33 766 811 940 2361 3046 4041 4232 33
Size: 733 45 129 1421 685 995 191 1121
Fragments arranged by size:
1421 1121 995 733 685 191 129 45
SfoI GGC'GCC
Cuts at: 2750 2998 2750
Size: 248 5072
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5320
SmaI CCC'GGG
Cuts at: 1741 2340 1741
Size: 599 4721
SmlI C'TyrA_G
Cuts at: 1414 1489 2203 2473 3388 3882 4144 1414
Size: 75 714 270 915 494 262 2590
Fragments arranged by size:
2590 915 714 494 270 262 75
SnaBI TAC'GTA
Cuts at: 2310 2310
Size: 5320
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5320
SphI G_CATG'C
Cuts at: 1779 2371 2871 1779
Size: 592 500 4228
Fragments arranged by size:
4228 592 500
StuI AGG'CCT
Cuts at: 1540 1567 1540
Size: 27 5293
StyI C'CwwG_G
Cuts at: 125 599 659 1125 1562 2452 2806 125
Size: 474 60 466 437 890 354 2639
Fragments arranged by size:
2639 890 474 466 437 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 1081 1085 2901 3034 3493 3704 262
Size: 819 4 1816 133 459 211 1878
Fragments arranged by size:
1878 1816 819 459 211 133 4
TatI w'GTAC_w
Cuts at: 1424 1901 4835 1424
Size: 477 2934 1909
Fragments arranged by size:
2934 1909 477
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 4837 4869 2163
Size: 32 2642 32 2614
Fragments arranged by size:
2642 2614 32 32
Tth111I GACn'n_nGTC
Cuts at: 3115 3115
Size: 5320
XbaI T'CTAG_A
Cuts at: 2349 2349
Size: 5320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1123 1743 1827 2813 1123
Size: 620 84 986 3630
Fragments arranged by size:
3630 986 620 84
XhoI C'TCGA_G
Cuts at: 1489 1489
Size: 5320
XmaI C'CCGG_G
Cuts at: 1739 2338 1739
Size: 599 4721
ZraI GAC'GTC
Cuts at: 720 927 1959 720
Size: 207 1032 4081
Fragments arranged by size:
4081 1032 207
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BsmI BssHII BstEII
BstZ17I Bsu36I CspCI DraIII EcoRV FseI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PasI PmlI
PsiI PsrI SacII SanDI SfiI SgrAI SrfI SspI
SwaI XmnI