Restriction map for
pF1KSDA0704
(Circular) (Six-base) MAPSORT of: pF1KSDA0704.seq Check: 870 from: 1 to: 5798
pF1KSDA0704 5798 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AatII G_ACGT'C
Cuts at: 860 860
Size: 5798
AccI GT'mk_AC
Cuts at: 216 1446 2836 216
Size: 1230 1390 3178
Fragments arranged by size:
3178 1390 1230
Acc65I G'GTAC_C
Cuts at: 2814 2814
Size: 5798
AclI AA'CG_TT
Cuts at: 5638 5638
Size: 5798
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 755 1228 1285 1499 3623 4055 4802 5070
Size: 232 473 57 214 2124 432 747 268
Cuts at: 5070 523
Size: 1251
Fragments arranged by size:
2124 1251 747 473 432 268 232 214
57
AflII C'TTAA_G
Cuts at: 2205 2205
Size: 5798
AflIII A'CryG_T
Cuts at: 3150 4254 5231 3150
Size: 1104 977 3717
Fragments arranged by size:
3717 1104 977
AgeI A'CCGG_T
Cuts at: 4186 4186
Size: 5798
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 830 830
Size: 5798
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2348 2380 2348
Size: 32 5766
AlwNI CAG_nnn'CTG
Cuts at: 843 3103 4670 843
Size: 2260 1567 1971
Fragments arranged by size:
2260 1971 1567
ApaLI G'TGCA_C
Cuts at: 4568 4568
Size: 5798
ApoI r'AATT_y
Cuts at: 696 2055 2092 2286 2802 5151 5407 696
Size: 1359 37 194 516 2349 256 1087
Fragments arranged by size:
2349 1359 1087 516 256 194 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 5798
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
AvaI C'yCGr_G
Cuts at: 1686 2818 1686
Size: 1132 4666
BamHI G'GATC_C
Cuts at: 2511 2823 2511
Size: 312 5486
BanI G'GyrC_C
Cuts at: 879 1677 2725 2814 3228 3476 3511 879
Size: 798 1048 89 414 248 35 3166
Fragments arranged by size:
3166 1048 798 414 248 89 35
BanII G_rGCy'C
Cuts at: 434 1419 1781 1829 2812 3842 434
Size: 985 362 48 983 1030 2390
Fragments arranged by size:
2390 1030 985 983 362 48
BbeI G_GCGC'C
Cuts at: 1681 3232 3480 1681
Size: 1551 248 3999
Fragments arranged by size:
3999 1551 248
BbsI GAAGACnn'nnnn_
Cuts at: 761 761
Size: 5798
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 196 230 408 442 2662 2696 196
Size: 34 178 34 2220 34 3298
Fragments arranged by size:
3298 2220 178 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3696 4457 5798 3696
Size: 761 1341 3696
Fragments arranged by size:
3696 1341 761
BclI T'GATC_A
Cuts at: 3318 3318
Size: 5798
BfrBI ATG'CAT
Cuts at: 467 1906 467
Size: 1439 4359
BglII A'GATC_T
Cuts at: 1128 1302 2748 3313 5350 1128
Size: 174 1446 565 2037 1576
Fragments arranged by size:
2037 1576 1446 565 174
BlpI GC'TnA_GC
Cuts at: 2910 2910
Size: 5798
Bme1580I G_kGCm'C
Cuts at: 3423 3516 4572 3423
Size: 93 1056 4649
Fragments arranged by size:
4649 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1012 3010 3091 3426 5570 1012
Size: 1998 81 335 2144 1240
Fragments arranged by size:
2144 1998 1240 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1413 4165 1413
Size: 2752 3046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1383 1834 2328 1383
Size: 451 494 4853
Fragments arranged by size:
4853 494 451
Bpu10I CC'TnA_GC
Cuts at: 1250 1487 1250
Size: 237 5561
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2974 3853 4345 4643 2974
Size: 879 492 298 4129
Fragments arranged by size:
4129 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5502 5502
Size: 5798
BsaAI yAC'GTr
Cuts at: 2790 3151 2790
Size: 361 5437
BsaBI GATnn'nnATC
Cuts at: 6 2245 3312 5349 6
Size: 2239 1067 2037 455
Fragments arranged by size:
2239 2037 1067 455
BsaHI Gr'CG_yC
Cuts at: 857 1678 3229 3477 4179 5675 857
Size: 821 1551 248 702 1496 980
Fragments arranged by size:
1551 1496 980 821 702 248
BsaWI w'CCGG_w
Cuts at: 1944 2987 3508 4186 4460 4607 5018 1944
Size: 1043 521 678 274 147 411 2724
Fragments arranged by size:
2724 1043 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 174 204 1801 1831 2228 2258 174
Size: 30 1597 30 397 30 3714
Fragments arranged by size:
3714 1597 397 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 1023 1224 1227 1336 1822 2406 499
Size: 524 201 3 109 486 584 3891
Fragments arranged by size:
3891 584 524 486 201 109 3
BseYI C'CCAG_C
Cuts at: 227 1852 3223 3981 4558 5054 227
Size: 1625 1371 758 577 496 971
Fragments arranged by size:
1625 1371 971 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 3340 3386 4594 104
Size: 3236 46 1208 1308
Fragments arranged by size:
3236 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 187 491 1419 1829 2231 2314 2675 2812 3590
Size: 304 928 410 402 83 361 137 778
Cuts at: 3590 3780 4572 187
Size: 190 792 1413
Fragments arranged by size:
1413 928 792 778 410 402 361 304
190 137 83
Bsp1286I G_dGCh'C
Cuts at: 187 434 491 1419 1781 1829 2231 2314 2675
Size: 247 57 928 362 48 402 83 361
Cuts at: 2675 2812 3423 3516 3590 3780 3842 4572 187
Size: 137 611 93 74 190 62 730 1413
Fragments arranged by size:
1413 928 730 611 402 362 361 247
190 137 93 83 74 62 57 48
BspEI T'CCGG_A
Cuts at: 5018 5018
Size: 5798
BspHI T'CATG_A
Cuts at: 4974 4974
Size: 5798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 984 2264 2848 3062 3364 3745 984
Size: 1280 584 214 302 381 3037
Fragments arranged by size:
3037 1280 584 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4090 4144 5628 5793 50
Size: 4040 54 1484 165 55
Fragments arranged by size:
4040 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 723 3710 723
Size: 2987 2811
BsrFI r'CCGG_y
Cuts at: 1674 1761 3796 3977 4186 1674
Size: 87 2035 181 209 3286
Fragments arranged by size:
3286 2035 209 181 87
BssSI C'ACGA_G
Cuts at: 746 4069 4427 746
Size: 3323 358 2117
Fragments arranged by size:
3323 2117 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1888 3175 1888
Size: 1287 4511
BstBI TT'CG_AA
Cuts at: 110 4159 110
Size: 4049 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 801 1764 1860 801
Size: 963 96 4739
Fragments arranged by size:
4739 963 96
BstYI r'GATC_y
Cuts at: 1 907 1128 1302 2511 2748 2823 3290 3313
Size: 906 221 174 1209 237 75 467 23
Cuts at: 3313 3648 3894 4906 4992 5004 5350 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1209 1012 906 467 449 346 335 246
237 221 174 86 75 23 12
Bsu36I CC'TnA_GG
Cuts at: 1720 1720
Size: 5798
BtgI C'CryG_G
Cuts at: 2108 2108
Size: 5798
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3748 3929 4092 3748
Size: 181 163 5454
Fragments arranged by size:
5454 181 163
BtsI GCAGTG_nn'
Cuts at: 988 3041 988
Size: 2053 3745
DraI TTT'AAA
Cuts at: 2797 2797
Size: 5798
DrdI GACnn_nn'nnGTC
Cuts at: 1546 2776 3504 4362 1546
Size: 1230 728 858 2982
Fragments arranged by size:
2982 1230 858 728
EaeI y'GGCC_r
Cuts at: 1754 3383 3557 3948 3975 5320 1754
Size: 1629 174 391 27 1345 2232
Fragments arranged by size:
2232 1629 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3383 3383
Size: 5798
EarI CTCTTCn'nnn_
Cuts at: 204 642 1064 1518 2424 3821 4031 204
Size: 438 422 454 906 1397 210 1971
Fragments arranged by size:
1971 1397 906 454 438 422 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1272 2012 4006 4316 4462 5608 1272
Size: 740 1994 310 146 1146 1462
Fragments arranged by size:
1994 1462 1146 740 310 146
EcoICRI GAG'CTC
Cuts at: 1417 1827 2810 1417
Size: 410 983 4405
Fragments arranged by size:
4405 983 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 755 1228 1285 1383 1499 1834 2328 3623
Size: 232 473 57 98 116 335 494 1295
Cuts at: 3623 4055 4802 5070 523
Size: 432 747 268 1251
Fragments arranged by size:
1295 1251 747 494 473 432 335 268
232 116 98 57
EcoNI CCTnn'n_nnAGG
Cuts at: 1747 1834 1747
Size: 87 5711
EcoO109I rG'GnC_Cy
Cuts at: 996 1621 1740 2937 996
Size: 625 119 1197 3857
Fragments arranged by size:
3857 1197 625 119
EcoRI G'AATT_C
Cuts at: 2802 2802
Size: 5798
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 336 360 1190 3817 336
Size: 24 830 2627 2317
Fragments arranged by size:
2627 2317 830 24
FspI TGC'GCA
Cuts at: 3579 3579
Size: 5798
HaeII r_GCGC'y
Cuts at: 1681 3232 3480 4502 5481 1681
Size: 1551 248 1022 979 1998
Fragments arranged by size:
1998 1551 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 625 657 1219 1251 1439 1471 1525
Size: 32 70 32 562 32 188 32 54
Cuts at: 1525 1557 2228 2260 2427 2459 2503 2535 2692
Size: 32 671 32 167 32 44 32 157
Cuts at: 2692 2724 3640 3670 3672 3702 523
Size: 32 916 30 2 30 2619
Fragments arranged by size:
2619 916 671 562 188 167 157 70
54 44 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 217 603 1052 2837 217
Size: 386 449 1785 3178
Fragments arranged by size:
3178 1785 449 386
HpaI GTT'AAC
Cuts at: 603 603
Size: 5798
KasI G'GCGC_C
Cuts at: 1677 3228 3476 1677
Size: 1551 248 3999
Fragments arranged by size:
3999 1551 248
KpnI G_GTAC'C
Cuts at: 2818 2818
Size: 5798
MfeI C'AATT_G
Cuts at: 2123 2123
Size: 5798
MluI A'CGCG_T
Cuts at: 5231 5231
Size: 5798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 4469 4653 2723
Size: 1746 184 3868
Fragments arranged by size:
3868 1746 184
MscI TGG'CCA
Cuts at: 1756 3559 1756
Size: 1803 3995
MslI CAynn'nnrTG
Cuts at: 462 812 1762 1965 3787 3914 462
Size: 350 950 203 1822 127 2346
Fragments arranged by size:
2346 1822 950 350 203 127
MspA1I CmG'CkG
Cuts at: 227 1192 1215 1360 2909 3223 3583 4596 4841
Size: 965 23 145 1549 314 360 1013 245
Cuts at: 4841 227
Size: 1184
Fragments arranged by size:
1549 1184 1013 965 360 314 245 145
23
NaeI GCC'GGC
Cuts at: 1676 3979 1676
Size: 2303 3495
NarI GG'CG_CC
Cuts at: 1678 3229 3477 1678
Size: 1551 248 3999
Fragments arranged by size:
3999 1551 248
NcoI C'CATG_G
Cuts at: 2108 2108
Size: 5798
NdeI CA'TA_TG
Cuts at: 1545 1962 1545
Size: 417 5381
NgoMIV G'CCGG_C
Cuts at: 1674 3977 1674
Size: 2303 3495
NsiI A_TGCA'T
Cuts at: 469 1908 469
Size: 1439 4359
NspI r_CATG'y
Cuts at: 2851 3351 4258 2851
Size: 500 907 4391
Fragments arranged by size:
4391 907 500
PciI A'CATG_T
Cuts at: 4254 4254
Size: 5798
PflMI CCAn_nnn'nTGG
Cuts at: 165 2155 165
Size: 1990 3808
PfoI T'CCnGG_A
Cuts at: 1654 1829 1654
Size: 175 5623
PmeI GTTT'AAAC
Cuts at: 2797 2797
Size: 5798
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1406 1438 4937 4969 1406
Size: 32 3499 32 2235
Fragments arranged by size:
3499 2235 32 32
PpuMI rG'GwC_Cy
Cuts at: 996 1740 996
Size: 744 5054
PsiI TTA'TAA
Cuts at: 2293 2293
Size: 5798
PstI C_TGCA'G
Cuts at: 995 1796 2633 2845 3530 995
Size: 801 837 212 685 3263
Fragments arranged by size:
3263 837 801 685 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5798
PvuII CAG'CTG
Cuts at: 227 1215 3223 3583 227
Size: 988 2008 360 2442
Fragments arranged by size:
2442 2008 988 360
RsrII CG'GwC_CG
Cuts at: 3993 3993
Size: 5798
SacI G_AGCT'C
Cuts at: 1419 1829 2812 1419
Size: 410 983 4405
Fragments arranged by size:
4405 983 410
SalI G'TCGA_C
Cuts at: 215 2835 215
Size: 2620 3178
SapI GCTCTTCn'nnn_
Cuts at: 204 1518 3821 4031 204
Size: 1314 2303 210 1971
Fragments arranged by size:
2303 1971 1314 210
SbfI CC_TGCA'GG
Cuts at: 2845 2845
Size: 5798
ScaI AGT'ACT
Cuts at: 5315 5315
Size: 5798
SexAI A'CCwGG_T
Cuts at: 821 821
Size: 5798
SfcI C'TryA_G
Cuts at: 33 991 1792 2472 2629 2841 3526 4519 4710
Size: 958 801 680 157 212 685 993 191
Cuts at: 4710 33
Size: 1121
Fragments arranged by size:
1121 993 958 801 685 680 212 191
157
SfoI GGC'GCC
Cuts at: 1679 3230 3478 1679
Size: 1551 248 3999
Fragments arranged by size:
3999 1551 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5798
SmaI CCC'GGG
Cuts at: 2820 2820
Size: 5798
SmlI C'TyrA_G
Cuts at: 2205 2953 3868 4360 4622 2205
Size: 748 915 492 262 3381
Fragments arranged by size:
3381 915 748 492 262
SnaBI TAC'GTA
Cuts at: 2790 2790
Size: 5798
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5798
SphI G_CATG'C
Cuts at: 2851 3351 2851
Size: 500 5298
StyI C'CwwG_G
Cuts at: 147 1616 1748 2108 2932 3286 147
Size: 1469 132 360 824 354 2659
Fragments arranged by size:
2659 1469 824 360 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 2703 3381 3514 3973 4182 283
Size: 2420 678 133 459 209 1899
Fragments arranged by size:
2420 1899 678 459 209 133
TatI w'GTAC_w
Cuts at: 1817 5313 1817
Size: 3496 2302
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 980 1012 5315 5347 980
Size: 32 4303 32 1431
Fragments arranged by size:
4303 1431 32 32
Tth111I GACn'n_nGTC
Cuts at: 3595 3595
Size: 5798
XbaI T'CTAG_A
Cuts at: 2050 2574 2829 2050
Size: 524 255 5019
Fragments arranged by size:
5019 524 255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3293 3293
Size: 5798
XmaI C'CCGG_G
Cuts at: 2818 2818
Size: 5798
XmnI GAAnn'nnTTC
Cuts at: 1970 2117 1970
Size: 147 5651
ZraI GAC'GTC
Cuts at: 858 858
Size: 5798
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI ApaI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII NheI NotI NruI PacI PasI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SspI StuI SwaI XhoI