Restriction map for
pF1KSDA0694
(Circular) (Six-base) MAPSORT of: pF1KSDA0694.seq Check: 2672 from: 1 to: 4918
pF1KSDA0694 4918 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1954 1954
Size: 4918
Acc65I G'GTAC_C
Cuts at: 1932 1932
Size: 4918
AclI AA'CG_TT
Cuts at: 1881 4758 1881
Size: 2877 2041
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1094 1324 1854 2741 3173 3922 4190 437
Size: 657 230 530 887 432 749 268 1165
Fragments arranged by size:
1165 887 749 657 530 432 268 230
AfeI AGC'GCT
Cuts at: 751 751
Size: 4918
AflIII A'CryG_T
Cuts at: 2268 3374 4351 2268
Size: 1106 977 2835
Fragments arranged by size:
2835 1106 977
AgeI A'CCGG_T
Cuts at: 3306 3306
Size: 4918
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1702 1734 1702
Size: 32 4886
AlwNI CAG_nnn'CTG
Cuts at: 415 2221 3790 415
Size: 1806 1569 1543
Fragments arranged by size:
1806 1569 1543
ApaI G_GGCC'C
Cuts at: 1013 1013
Size: 4918
ApaLI G'TGCA_C
Cuts at: 3688 3688
Size: 4918
ApoI r'AATT_y
Cuts at: 441 466 699 1591 1920 4271 4527 441
Size: 25 233 892 329 2351 256 832
Fragments arranged by size:
2351 892 832 329 256 233 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 4918
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4918
AvaI C'yCGr_G
Cuts at: 268 539 1936 268
Size: 271 1397 3250
Fragments arranged by size:
3250 1397 271
AvrII C'CTAG_G
Cuts at: 259 259
Size: 4918
BamHI G'GATC_C
Cuts at: 968 1941 968
Size: 973 3945
BanI G'GyrC_C
Cuts at: 1690 1932 2346 2594 2629 1690
Size: 242 414 248 35 3979
Fragments arranged by size:
3979 414 248 242 35
BanII G_rGCy'C
Cuts at: 191 1013 1040 1930 2960 191
Size: 822 27 890 1030 2149
Fragments arranged by size:
2149 1030 890 822 27
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4670
BbsI GAAGACnn'nnnn_
Cuts at: 626 626
Size: 4918
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3577 4918 2814
Size: 763 1341 2814
Fragments arranged by size:
2814 1341 763
BclI T'GATC_A
Cuts at: 1271 1310 2436 1271
Size: 39 1126 3753
Fragments arranged by size:
3753 1126 39
BfrBI ATG'CAT
Cuts at: 839 839
Size: 4918
BglI GCCn_nnn'nGGC
Cuts at: 220 220
Size: 4918
BglII A'GATC_T
Cuts at: 336 1248 2431 4470 336
Size: 912 1183 2039 784
Fragments arranged by size:
2039 1183 912 784
BlpI GC'TnA_GC
Cuts at: 2028 2028
Size: 4918
Bme1580I G_kGCm'C
Cuts at: 1013 2541 2634 3692 1013
Size: 1528 93 1058 2239
Fragments arranged by size:
2239 1528 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 347 474 2128 2209 2544 4690 347
Size: 127 1654 81 335 2146 575
Fragments arranged by size:
2146 1654 575 335 127 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3283 3283
Size: 4918
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 539
Size: 4918
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2971 3465 3763 2092
Size: 879 494 298 3247
Fragments arranged by size:
3247 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 162 4622 162
Size: 4460 458
BsaAI yAC'GTr
Cuts at: 1908 2269 1908
Size: 361 4557
BsaBI GATnn'nnATC
Cuts at: 6 2430 4469 6
Size: 2424 2039 455
Fragments arranged by size:
2424 2039 455
BsaHI Gr'CG_yC
Cuts at: 2347 2595 3299 4795 2347
Size: 248 704 1496 2470
Fragments arranged by size:
2470 1496 704 248
BsaWI w'CCGG_w
Cuts at: 317 2105 2626 3306 3580 3727 4138 317
Size: 1788 521 680 274 147 411 1097
Fragments arranged by size:
1788 1097 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1521 1551 1702 1732 1521
Size: 30 151 30 4707
Fragments arranged by size:
4707 151 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 665 1038 1041 665
Size: 373 3 4542
Fragments arranged by size:
4542 373 3
BseYI C'CCAG_C
Cuts at: 2341 3099 3678 4174 2341
Size: 758 579 496 3085
Fragments arranged by size:
3085 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 889 889
Size: 4918
BsiEI CG_ry'CG
Cuts at: 104 185 2458 2504 3714 104
Size: 81 2273 46 1210 1308
Fragments arranged by size:
2273 1308 1210 81 46
BsiHKAI G_wGCw'C
Cuts at: 191 431 1033 1040 1930 2708 2898 3692 191
Size: 240 602 7 890 778 190 794 1417
Fragments arranged by size:
1417 890 794 778 602 240 190 7
BsmI GAATG_Cn'
Cuts at: 986 1090 986
Size: 104 4814
Bsp1286I G_dGCh'C
Cuts at: 191 431 1013 1033 1040 1930 2541 2634 2708
Size: 240 582 20 7 890 611 93 74
Cuts at: 2708 2898 2960 3692 191
Size: 190 62 732 1417
Fragments arranged by size:
1417 890 732 611 582 240 190 93
74 62 20 7
BspEI T'CCGG_A
Cuts at: 4138 4138
Size: 4918
BspHI T'CATG_A
Cuts at: 1307 4094 1307
Size: 2787 2131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 137 1966 2180 2482 2863 137
Size: 1829 214 302 381 2192
Fragments arranged by size:
2192 1829 381 302 214
BsrBI CCG'CTC
Cuts at: 50 138 655 3208 3262 4748 4913 50
Size: 88 517 2553 54 1486 165 55
Fragments arranged by size:
2553 1486 517 165 88 55 54
BsrDI GCAATG_nn'
Cuts at: 1679 2828 1679
Size: 1149 3769
BsrFI r'CCGG_y
Cuts at: 130 233 2914 3095 3306 130
Size: 103 2681 181 211 1742
Fragments arranged by size:
2681 1742 211 181 103
BsrGI T'GTAC_A
Cuts at: 856 856
Size: 4918
BssSI C'ACGA_G
Cuts at: 3187 3547 3187
Size: 360 4558
BstAPI GCAn_nnn'nTGC
Cuts at: 1135 1690 2293 1135
Size: 555 603 3760
Fragments arranged by size:
3760 603 555
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4918
BstXI CCAn_nnnn'nTGG
Cuts at: 1583 1583
Size: 4918
BstYI r'GATC_y
Cuts at: 1 336 968 1212 1248 1458 1941 2408 2431
Size: 335 632 244 36 210 483 467 23
Cuts at: 2431 2766 3012 4026 4112 4124 4470 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 632 483 467 449 346 335 335
246 244 210 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 1004 1004
Size: 4918
BtgI C'CryG_G
Cuts at: 125 215 125
Size: 90 4828
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4574
Fragments arranged by size:
4574 181 163
BtsI GCAGTG_nn'
Cuts at: 2159 2159
Size: 4918
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 627 662 627
Size: 35 4883
DraI TTT'AAA
Cuts at: 1915 1915
Size: 4918
DrdI GACnn_nn'nnGTC
Cuts at: 2622 3482 2622
Size: 860 4058
EaeI y'GGCC_r
Cuts at: 128 182 1649 2501 2675 3066 3093 4440 128
Size: 54 1467 852 174 391 27 1347 606
Fragments arranged by size:
1467 1347 852 606 391 174 54 27
EagI C'GGCC_G
Cuts at: 182 2501 182
Size: 2319 2599
EarI CTCTTCn'nnn_
Cuts at: 335 2939 3149 335
Size: 2604 210 2104
Fragments arranged by size:
2604 2104 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 198 3124 3436 3582 4728 198
Size: 2926 312 146 1146 388
Fragments arranged by size:
2926 1146 388 312 146
EcoICRI GAG'CTC
Cuts at: 189 1038 1928 189
Size: 849 890 3179
Fragments arranged by size:
3179 890 849
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 539 1094 1324 1854 2741 3173 3922 4190
Size: 102 555 230 530 887 432 749 268
Cuts at: 4190 437
Size: 1165
Fragments arranged by size:
1165 887 749 555 530 432 268 230
102
EcoO109I rG'GnC_Cy
Cuts at: 1009 1010 2055 1009
Size: 1 1045 3872
Fragments arranged by size:
3872 1045 1
EcoRI G'AATT_C
Cuts at: 1920 1920
Size: 4918
EcoRV GAT'ATC
Cuts at: 390 390
Size: 4918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 771 2935 771
Size: 2164 2754
FspI TGC'GCA
Cuts at: 2697 2697
Size: 4918
HaeII r_GCGC'y
Cuts at: 203 753 2350 2598 3622 4601 203
Size: 550 1597 248 1024 979 520
Fragments arranged by size:
1597 1024 979 550 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 802 834 1182 1214 1472 1504 2758 2788 2790
Size: 32 348 32 258 32 1254 30 2
Cuts at: 2790 2820 802
Size: 30 2900
Fragments arranged by size:
2900 1254 348 258 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1818 1955 1818
Size: 137 4781
HindIII A'AGCT_T
Cuts at: 562 1866 562
Size: 1304 3614
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4670
KpnI G_GTAC'C
Cuts at: 1936 1936
Size: 4918
MluI A'CGCG_T
Cuts at: 4351 4351
Size: 4918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1745 1775 3589 3773 1745
Size: 30 1814 184 2890
Fragments arranged by size:
2890 1814 184 30
MscI TGG'CCA
Cuts at: 1651 2677 1651
Size: 1026 3892
MslI CAynn'nnrTG
Cuts at: 596 2905 3032 596
Size: 2309 127 2482
Fragments arranged by size:
2482 2309 127
MspA1I CmG'CkG
Cuts at: 217 246 762 940 2027 2341 2701 3716 3961
Size: 29 516 178 1087 314 360 1015 245
Cuts at: 3961 217
Size: 1174
Fragments arranged by size:
1174 1087 1015 516 360 314 245 178
29
NaeI GCC'GGC
Cuts at: 235 3097 235
Size: 2862 2056
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4670
NcoI C'CATG_G
Cuts at: 125 125
Size: 4918
NgoMIV G'CCGG_C
Cuts at: 233 3095 233
Size: 2862 2056
NruI TCG'CGA
Cuts at: 3279 3279
Size: 4918
NsiI A_TGCA'T
Cuts at: 841 841
Size: 4918
NspI r_CATG'y
Cuts at: 490 1969 2469 3378 490
Size: 1479 500 909 2030
Fragments arranged by size:
2030 1479 909 500
PciI A'CATG_T
Cuts at: 3374 3374
Size: 4918
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4918
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1702 1734 4057 4089 1702
Size: 32 2323 32 2531
Fragments arranged by size:
2531 2323 32 32
PspOMI G'GGCC_C
Cuts at: 1009 1009
Size: 4918
PstI C_TGCA'G
Cuts at: 1768 1963 2648 1768
Size: 195 685 4038
Fragments arranged by size:
4038 685 195
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4918
PvuII CAG'CTG
Cuts at: 762 940 2341 2701 762
Size: 178 1401 360 2979
Fragments arranged by size:
2979 1401 360 178
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4918
SacI G_AGCT'C
Cuts at: 191 1040 1930 191
Size: 849 890 3179
Fragments arranged by size:
3179 890 849
SacII CC_GC'GG
Cuts at: 218 218
Size: 4918
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4918
SapI GCTCTTCn'nnn_
Cuts at: 2939 3149 2939
Size: 210 4708
SbfI CC_TGCA'GG
Cuts at: 1963 1963
Size: 4918
ScaI AGT'ACT
Cuts at: 4435 4435
Size: 4918
SfcI C'TryA_G
Cuts at: 33 1764 1959 2644 3639 3830 33
Size: 1731 195 685 995 191 1121
Fragments arranged by size:
1731 1121 995 685 195 191
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4670
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4918
SmaI CCC'GGG
Cuts at: 1938 1938
Size: 4918
SmlI C'TyrA_G
Cuts at: 268 2071 2986 3480 3742 268
Size: 1803 915 494 262 1444
Fragments arranged by size:
1803 1444 915 494 262
SnaBI TAC'GTA
Cuts at: 1908 1908
Size: 4918
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4918
SphI G_CATG'C
Cuts at: 1969 2469 1969
Size: 500 4418
SspI AAT'ATT
Cuts at: 514 1383 514
Size: 869 4049
StyI C'CwwG_G
Cuts at: 125 259 638 1017 2050 2404 125
Size: 134 379 379 1033 354 2639
Fragments arranged by size:
2639 1033 379 379 354 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 725 2499 2632 3091 3302 725
Size: 1774 133 459 211 2341
Fragments arranged by size:
2341 1774 459 211 133
TatI w'GTAC_w
Cuts at: 401 856 4433 401
Size: 455 3577 886
Fragments arranged by size:
3577 886 455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 1521 1553 4435 4467 619
Size: 32 870 32 2882 32 1070
Fragments arranged by size:
2882 1070 870 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2713 2713
Size: 4918
XbaI T'CTAG_A
Cuts at: 1209 1947 1209
Size: 738 4180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2411 2411
Size: 4918
XhoI C'TCGA_G
Cuts at: 268 268
Size: 4918
XmaI C'CCGG_G
Cuts at: 1936 1936
Size: 4918
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NsiI NspI PciI PmeI PpiI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AscI BaeI
BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstZ17I ClaI DraIII EcoNI FseI FspAI HpaI
MfeI NdeI NheI NotI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SrfI StuI SwaI XmnI ZraI