Restriction map for
pF1KSDA0690
(Circular) (Six-base) MAPSORT of: pF1KSDA0690.seq Check: 4432 from: 1 to: 6730
pF1KSDA0690 6730 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3711 3766 3711
Size: 55 6675
Acc65I G'GTAC_C
Cuts at: 403 3744 403
Size: 3341 3389
AclI AA'CG_TT
Cuts at: 6570 6570
Size: 6730
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 429 778 1174 1633 1886 2296 4553 4985
Size: 195 349 396 459 253 410 2257 432
Cuts at: 4985 5734 6002 234
Size: 749 268 962
Fragments arranged by size:
2257 962 749 459 432 410 396 349
268 253 195
AfeI AGC'GCT
Cuts at: 175 460 175
Size: 285 6445
AflII C'TTAA_G
Cuts at: 997 997
Size: 6730
AflIII A'CryG_T
Cuts at: 2766 4080 5186 6163 2766
Size: 1314 1106 977 3333
Fragments arranged by size:
3333 1314 1106 977
AgeI A'CCGG_T
Cuts at: 5118 5118
Size: 6730
AleI CACnn'nnGTG
Cuts at: 523 1680 523
Size: 1157 5573
AlwNI CAG_nnn'CTG
Cuts at: 1216 1305 1371 1818 1855 2655 2737 4033 5602
Size: 89 66 447 37 800 82 1296 1569
Cuts at: 5602 1216
Size: 2344
Fragments arranged by size:
2344 1569 1296 800 447 89 82 66
37
ApaI G_GGCC'C
Cuts at: 2239 2247 2239
Size: 8 6722
ApaLI G'TGCA_C
Cuts at: 2418 5500 2418
Size: 3082 3648
ApoI r'AATT_y
Cuts at: 628 1270 3732 6083 6339 628
Size: 642 2462 2351 256 1019
Fragments arranged by size:
2462 2351 1019 642 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6730
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6730
AvaI C'yCGr_G
Cuts at: 135 399 2239 2899 2945 3748 135
Size: 264 1840 660 46 803 3117
Fragments arranged by size:
3117 1840 803 660 264 46
BamHI G'GATC_C
Cuts at: 3189 3570 3753 3189
Size: 381 183 6166
Fragments arranged by size:
6166 381 183
BanI G'GyrC_C
Cuts at: 403 2957 3334 3744 4158 4406 4441 403
Size: 2554 377 410 414 248 35 2692
Fragments arranged by size:
2692 2554 414 410 377 248 35
BanII G_rGCy'C
Cuts at: 923 1291 2239 2247 2967 3020 3176 3742 4772
Size: 368 948 8 720 53 156 566 1030
Cuts at: 4772 923
Size: 2881
Fragments arranged by size:
2881 1030 948 720 566 368 156 53
8
BbeI G_GCGC'C
Cuts at: 4162 4410 4162
Size: 248 6482
BbsI GAAGACnn'nnnn_
Cuts at: 1305 2287 1305
Size: 982 5748
BbvCI CC'TCA_GC
Cuts at: 2939 3428 2939
Size: 489 6241
BciVI GTATCCnnnnn_n'
Cuts at: 4626 5389 6730 4626
Size: 763 1341 4626
Fragments arranged by size:
4626 1341 763
BclI T'GATC_A
Cuts at: 3287 3380 4248 3287
Size: 93 868 5769
Fragments arranged by size:
5769 868 93
BglI GCCn_nnn'nGGC
Cuts at: 865 2558 3502 865
Size: 1693 944 4093
Fragments arranged by size:
4093 1693 944
BglII A'GATC_T
Cuts at: 494 1664 4243 6282 494
Size: 1170 2579 2039 942
Fragments arranged by size:
2579 2039 1170 942
BlpI GC'TnA_GC
Cuts at: 3840 3840
Size: 6730
Bme1580I G_kGCm'C
Cuts at: 2239 2247 2422 4353 4446 5504 2239
Size: 8 175 1931 93 1058 3465
Fragments arranged by size:
3465 1931 1058 175 93 8
BmgBI CAC'GTC
Cuts at: 2697 2748 2697
Size: 51 6679
BmrI ACTGGGnnnn_n'
Cuts at: 956 2722 3940 4021 4356 6502 956
Size: 1766 1218 81 335 2146 1184
Fragments arranged by size:
2146 1766 1218 1184 335 81
BmtI G_CTAG'C
Cuts at: 991 991
Size: 6730
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 414 492 5095 414
Size: 78 4603 2049
Fragments arranged by size:
4603 2049 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 731 1305 1348 1663 1928 2230 2287 2677 3463
Size: 574 43 315 265 302 57 390 786
Cuts at: 3463 3488 731
Size: 25 3973
Fragments arranged by size:
3973 786 574 390 315 302 265 57
43 25
Bpu10I CC'TnA_GC
Cuts at: 901 1637 2939 2968 3428 901
Size: 736 1302 29 460 4203
Fragments arranged by size:
4203 1302 736 460 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 852 1126 3394 3904 4783 5277 5575 601
Size: 251 274 2268 510 879 494 298 1756
Fragments arranged by size:
2268 1756 879 510 494 298 274 251
BsaI GGTCTCn'nnnn_
Cuts at: 1541 6434 1541
Size: 4893 1837
BsaAI yAC'GTr
Cuts at: 3720 4081 3720
Size: 361 6369
BsaBI GATnn'nnATC
Cuts at: 6 4242 6281 6
Size: 4236 2039 455
Fragments arranged by size:
4236 2039 455
BsaHI Gr'CG_yC
Cuts at: 355 4159 4407 5111 6607 355
Size: 3804 248 704 1496 478
Fragments arranged by size:
3804 1496 704 478 248
BsaWI w'CCGG_w
Cuts at: 581 2930 3917 4438 5118 5392 5539 5950 581
Size: 2349 987 521 680 274 147 411 1361
Fragments arranged by size:
2349 1361 987 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 1174 1204 2203 2233 2650 2680 547
Size: 30 597 30 999 30 417 30 4597
Fragments arranged by size:
4597 999 597 417 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 1191 1267 1516 2227 2301 2917 3001 3013
Size: 386 76 249 711 74 616 84 12
Cuts at: 3013 3016 3019 3022 3025 3028 3061 3088 3100
Size: 3 3 3 3 3 33 27 12
Cuts at: 3100 3103 3150 3313 3445 3448 3451 805
Size: 3 47 163 132 3 3 4084
Fragments arranged by size:
4084 711 616 386 249 163 132 84
76 74 47 33 27 12 12 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 593 1764 4153 4911 5490 5986 593
Size: 1171 2389 758 579 496 1337
Fragments arranged by size:
2389 1337 1171 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 414 724 1698 2206 2275 2693 2791 414
Size: 310 974 508 69 418 98 4353
Fragments arranged by size:
4353 974 508 418 310 98 69
BsiEI CG_ry'CG
Cuts at: 104 259 3568 4270 4316 5526 104
Size: 155 3309 702 46 1210 1308
Fragments arranged by size:
3309 1308 1210 702 155 46
BsiHKAI G_wGCw'C
Cuts at: 907 923 1291 2048 2308 2422 3742 4520 4710
Size: 16 368 757 260 114 1320 778 190
Cuts at: 4710 5504 907
Size: 794 2133
Fragments arranged by size:
2133 1320 794 778 757 368 260 190
114 16
BsmI GAATG_Cn'
Cuts at: 1165 3479 1165
Size: 2314 4416
BsmBI CGTCTCn'nnnn_
Cuts at: 346 544 346
Size: 198 6532
Bsp1286I G_dGCh'C
Cuts at: 907 923 1291 2048 2239 2247 2308 2422 2967
Size: 16 368 757 191 8 61 114 545
Cuts at: 2967 3020 3176 3742 4353 4446 4520 4710 4772
Size: 53 156 566 611 93 74 190 62
Cuts at: 4772 5504 907
Size: 732 2133
Fragments arranged by size:
2133 757 732 611 566 545 368 191
190 156 114 93 74 62 61 53
16 8
BspEI T'CCGG_A
Cuts at: 581 2930 5950 581
Size: 2349 3020 1361
Fragments arranged by size:
3020 2349 1361
BspHI T'CATG_A
Cuts at: 824 5906 824
Size: 5082 1648
BspMI ACCTGCnnnn'nnnn_
Cuts at: 773 1099 1388 3778 3992 4294 4675 773
Size: 326 289 2390 214 302 381 2828
Fragments arranged by size:
2828 2390 381 326 302 289 214
BsrBI CCG'CTC
Cuts at: 50 5020 5074 6560 6725 50
Size: 4970 54 1486 165 55
Fragments arranged by size:
4970 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4640 4640
Size: 6730
BsrFI r'CCGG_y
Cuts at: 254 3281 3563 4726 4907 5118 254
Size: 3027 282 1163 181 211 1866
Fragments arranged by size:
3027 1866 1163 282 211 181
BssSI C'ACGA_G
Cuts at: 2467 4999 5359 2467
Size: 2532 360 3838
Fragments arranged by size:
3838 2532 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1476 4105 1476
Size: 2629 4101
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6730
BstEII G'GTnAC_C
Cuts at: 1086 1440 1800 2565 1086
Size: 354 360 765 5251
Fragments arranged by size:
5251 765 360 354
BstXI CCAn_nnnn'nTGG
Cuts at: 1526 1632 2643 1526
Size: 106 1011 5613
Fragments arranged by size:
5613 1011 106
BstYI r'GATC_y
Cuts at: 1 494 1148 1664 3189 3570 3753 4220 4243
Size: 493 654 516 1525 381 183 467 23
Cuts at: 4243 4578 4824 5838 5924 5936 6282 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1525 1014 654 516 493 467 449 381
346 335 246 183 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 817 1209 1457 1795 2231 2491 817
Size: 392 248 338 436 260 5056
Fragments arranged by size:
5056 436 392 338 260 248
BtgI C'CryG_G
Cuts at: 125 359 1519 1625 1925 2178 2570 2981 125
Size: 234 1160 106 300 253 392 411 3874
Fragments arranged by size:
3874 1160 411 392 300 253 234 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3469 4678 4859 5022 3469
Size: 1209 181 163 5177
Fragments arranged by size:
5177 1209 181 163
BtsI GCAGTG_nn'
Cuts at: 630 674 810 1045 1253 1433 2284 2390 2461
Size: 44 136 235 208 180 851 106 71
Cuts at: 2461 2530 2738 3971 630
Size: 69 208 1233 3389
Fragments arranged by size:
3389 1233 851 235 208 208 180 136
106 71 69 44
DraI TTT'AAA
Cuts at: 2619 3727 2619
Size: 1108 5622
DraIII CAC_nnn'GTG
Cuts at: 1347 1808 2184 2355 1347
Size: 461 376 171 5722
Fragments arranged by size:
5722 461 376 171
DrdI GACnn_nn'nnGTC
Cuts at: 280 1798 4434 5294 280
Size: 1518 2636 860 1716
Fragments arranged by size:
2636 1716 1518 860
EaeI y'GGCC_r
Cuts at: 256 1733 1772 2759 3215 3279 3494 3565 4313
Size: 1477 39 987 456 64 215 71 748
Cuts at: 4313 4487 4878 4905 6252 256
Size: 174 391 27 1347 734
Fragments arranged by size:
1477 1347 987 748 734 456 391 215
174 71 64 39 27
EagI C'GGCC_G
Cuts at: 256 3565 4313 256
Size: 3309 748 2673
Fragments arranged by size:
3309 2673 748
EarI CTCTTCn'nnn_
Cuts at: 362 790 886 1609 1909 2254 2507 2990 3320
Size: 428 96 723 300 345 253 483 330
Cuts at: 3320 3347 4751 4961 362
Size: 27 1404 210 2131
Fragments arranged by size:
2131 1404 723 483 428 345 330 300
253 210 96 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 388 4936 5248 5394 6540 388
Size: 4548 312 146 1146 578
Fragments arranged by size:
4548 1146 578 312 146
EcoICRI GAG'CTC
Cuts at: 921 1289 3740 921
Size: 368 2451 3911
Fragments arranged by size:
3911 2451 368
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 429 731 778 1174 1305 1348 1633 1663
Size: 195 302 47 396 131 43 285 30
Cuts at: 1663 1886 1928 2230 2287 2296 2677 3463 3488
Size: 223 42 302 57 9 381 786 25
Cuts at: 3488 4553 4985 5734 6002 234
Size: 1065 432 749 268 962
Fragments arranged by size:
1065 962 786 749 432 396 381 302
302 285 268 223 195 131 57 47
43 42 30 25 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1636 2971 3655 1636
Size: 1335 684 4711
Fragments arranged by size:
4711 1335 684
EcoO109I rG'GnC_Cy
Cuts at: 320 645 762 1044 1213 1257 1453 1815 2235
Size: 325 117 282 169 44 196 362 420
Cuts at: 2235 2236 2243 2244 2271 2327 2517 3867 320
Size: 1 7 1 27 56 190 1350 3183
Fragments arranged by size:
3183 1350 420 362 325 282 196 190
169 117 56 44 27 7 1 1
EcoRI G'AATT_C
Cuts at: 628 3732 628
Size: 3104 3626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4747 4747
Size: 6730
FseI GG_CCGG'CC
Cuts at: 3567 3567
Size: 6730
FspI TGC'GCA
Cuts at: 1822 4509 1822
Size: 2687 4043
HaeII r_GCGC'y
Cuts at: 177 462 3275 4162 4410 5434 6413 177
Size: 285 2813 887 248 1024 979 494
Fragments arranged by size:
2813 1024 979 887 494 285 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 1174 1206 2066 2098 2906 2938 3438
Size: 32 844 32 860 32 808 32 500
Cuts at: 3438 3470 4570 4600 4602 4632 298
Size: 32 1100 30 2 30 2396
Fragments arranged by size:
2396 1100 860 844 808 500 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2925 3712 3767 2925
Size: 787 55 5888
Fragments arranged by size:
5888 787 55
HindIII A'AGCT_T
Cuts at: 2797 2833 2797
Size: 36 6694
KasI G'GCGC_C
Cuts at: 4158 4406 4158
Size: 248 6482
KpnI G_GTAC'C
Cuts at: 407 3748 407
Size: 3341 3389
MluI A'CGCG_T
Cuts at: 6163 6163
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 449 497 5401 5585 449
Size: 48 4904 184 1594
Fragments arranged by size:
4904 1594 184 48
MscI TGG'CCA
Cuts at: 1735 1774 2761 3217 3496 4489 1735
Size: 39 987 456 279 993 3976
Fragments arranged by size:
3976 993 987 456 279 39
MslI CAynn'nnrTG
Cuts at: 523 1680 2725 2746 4717 4844 523
Size: 1157 1045 21 1971 127 2409
Fragments arranged by size:
2409 1971 1157 1045 127 21
MspA1I CmG'CkG
Cuts at: 1302 2371 2579 2652 2775 3117 3839 4153 4513
Size: 1069 208 73 123 342 722 314 360
Cuts at: 4513 5528 5773 1302
Size: 1015 245 2259
Fragments arranged by size:
2259 1069 1015 722 360 342 314 245
208 123 73
NaeI GCC'GGC
Cuts at: 256 3283 3565 4909 256
Size: 3027 282 1344 2077
Fragments arranged by size:
3027 2077 1344 282
NarI GG'CG_CC
Cuts at: 4159 4407 4159
Size: 248 6482
NcoI C'CATG_G
Cuts at: 125 359 1625 2570 125
Size: 234 1266 945 4285
Fragments arranged by size:
4285 1266 945 234
NgoMIV G'CCGG_C
Cuts at: 254 3281 3563 4907 254
Size: 3027 282 1344 2077
Fragments arranged by size:
3027 2077 1344 282
NheI G'CTAG_C
Cuts at: 987 987
Size: 6730
NruI TCG'CGA
Cuts at: 5091 5091
Size: 6730
NspI r_CATG'y
Cuts at: 747 2770 2814 3781 4281 5190 747
Size: 2023 44 967 500 909 2287
Fragments arranged by size:
2287 2023 967 909 500 44
PasI CC'CwG_GG
Cuts at: 537 3024 537
Size: 2487 4243
PciI A'CATG_T
Cuts at: 2766 5186 2766
Size: 2420 4310
PflMI CCAn_nnn'nTGG
Cuts at: 843 1428 843
Size: 585 6145
PfoI T'CCnGG_A
Cuts at: 692 779 2021 2084 3185 692
Size: 87 1242 63 1101 4237
Fragments arranged by size:
4237 1242 1101 87 63
PmeI GTTT'AAAC
Cuts at: 3727 3727
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1538 1570 5869 5901 1538
Size: 32 4299 32 2367
Fragments arranged by size:
4299 2367 32 32
PpuMI rG'GwC_Cy
Cuts at: 320 762 1044 1453 2271 320
Size: 442 282 409 818 4779
Fragments arranged by size:
4779 818 442 409 282
PshAI GACnn'nnGTC
Cuts at: 2572 2572
Size: 6730
PspOMI G'GGCC_C
Cuts at: 2235 2243 2235
Size: 8 6722
PstI C_TGCA'G
Cuts at: 314 805 1472 2525 2584 3635 3775 4460 314
Size: 491 667 1053 59 1051 140 685 2584
Fragments arranged by size:
2584 1053 1051 685 667 491 140 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6730
PvuII CAG'CTG
Cuts at: 1302 2371 2579 2652 2775 4153 4513 1302
Size: 1069 208 73 123 1378 360 3519
Fragments arranged by size:
3519 1378 1069 360 208 123 73
RsrII CG'GwC_CG
Cuts at: 4923 4923
Size: 6730
SacI G_AGCT'C
Cuts at: 923 1291 3742 923
Size: 368 2451 3911
Fragments arranged by size:
3911 2451 368
SalI G'TCGA_C
Cuts at: 3710 3765 3710
Size: 55 6675
SapI GCTCTTCn'nnn_
Cuts at: 790 1609 4751 4961 790
Size: 819 3142 210 2559
Fragments arranged by size:
3142 2559 819 210
SbfI CC_TGCA'GG
Cuts at: 3775 3775
Size: 6730
ScaI AGT'ACT
Cuts at: 565 6247 565
Size: 5682 1048
SexAI A'CCwGG_T
Cuts at: 1025 2090 1025
Size: 1065 5665
SfcI C'TryA_G
Cuts at: 33 310 801 1468 1724 2521 2580 3631 3771
Size: 277 491 667 256 797 59 1051 140
Cuts at: 3771 4456 5451 5642 33
Size: 685 995 191 1121
Fragments arranged by size:
1121 1051 995 797 685 667 491 277
256 191 140 59
SfoI GGC'GCC
Cuts at: 4160 4408 4160
Size: 248 6482
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6730
SmaI CCC'GGG
Cuts at: 2241 2947 3750 2241
Size: 706 803 5221
Fragments arranged by size:
5221 803 706
SmlI C'TyrA_G
Cuts at: 616 831 997 1141 3409 3883 4798 5292 5554
Size: 215 166 144 2268 474 915 494 262
Cuts at: 5554 616
Size: 1792
Fragments arranged by size:
2268 1792 915 494 474 262 215 166
144
SnaBI TAC'GTA
Cuts at: 3720 3720
Size: 6730
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6730
SphI G_CATG'C
Cuts at: 3781 4281 3781
Size: 500 6230
SrfI GCCC'GGGC
Cuts at: 2947 2947
Size: 6730
StuI AGG'CCT
Cuts at: 788 868 894 1722 2197 3650 788
Size: 80 26 828 475 1453 3868
Fragments arranged by size:
3868 1453 828 475 80 26
StyI C'CwwG_G
Cuts at: 125 359 719 1625 1835 2102 2423 2570 3194
Size: 234 360 906 210 267 321 147 624
Cuts at: 3194 3862 4216 125
Size: 668 354 2639
Fragments arranged by size:
2639 906 668 624 360 354 321 267
234 210 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4311 4444 4903 5114 4311
Size: 133 459 211 5927
Fragments arranged by size:
5927 459 211 133
TatI w'GTAC_w
Cuts at: 453 563 2638 6245 453
Size: 110 2075 3607 938
Fragments arranged by size:
3607 2075 938 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1070 1102 1445 1477 2203 2235 2650 2682 6247
Size: 32 343 32 726 32 415 32 3565
Cuts at: 6247 6279 1070
Size: 32 1521
Fragments arranged by size:
3565 1521 726 415 343 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2092 2698 4525 2092
Size: 606 1827 4297
Fragments arranged by size:
4297 1827 606
XbaI T'CTAG_A
Cuts at: 3759 3759
Size: 6730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 892 4223 892
Size: 3331 3399
XmaI C'CCGG_G
Cuts at: 2239 2945 3748 2239
Size: 706 803 5221
Fragments arranged by size:
5221 803 706
XmnI GAAnn'nnTTC
Cuts at: 2845 2845
Size: 6730
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AlfI AloI AscI AvrII BaeI
BcgI BfrBI BsiWI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FspAI HpaI MfeI NdeI NotI NsiI PacI
PmlI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SspI SwaI XhoI ZraI