Restriction map for
pF1KSDA0688
(Circular) (Six-base) MAPSORT of: pF1KSDA0688.seq Check: 5691 from: 1 to: 5650
pF1KSDA0688 5650 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1999 1999
Size: 5650
AccI GT'mk_AC
Cuts at: 2686 2686
Size: 5650
Acc65I G'GTAC_C
Cuts at: 2664 2664
Size: 5650
AclI AA'CG_TT
Cuts at: 5490 5490
Size: 5650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2184 2332 3473 3905 4654 4922 2184
Size: 148 1141 432 749 268 2912
Fragments arranged by size:
2912 1141 749 432 268 148
AfeI AGC'GCT
Cuts at: 760 844 760
Size: 84 5566
AflIII A'CryG_T
Cuts at: 3000 4106 5083 3000
Size: 1106 977 3567
Fragments arranged by size:
3567 1106 977
AgeI A'CCGG_T
Cuts at: 4038 4038
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1960 2374 1960
Size: 414 5236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 355 1641 1904 355
Size: 1286 263 4101
Fragments arranged by size:
4101 1286 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 593 625 2040 2072 593
Size: 32 1415 32 4171
Fragments arranged by size:
4171 1415 32 32
AlwNI CAG_nnn'CTG
Cuts at: 459 496 618 990 1001 1086 2598 2953 4522
Size: 37 122 372 11 85 1512 355 1569
Cuts at: 4522 459
Size: 1587
Fragments arranged by size:
1587 1569 1512 372 355 122 85 37
11
ApaI G_GGCC'C
Cuts at: 962 974 1490 1616 1934 962
Size: 12 516 126 318 4678
Fragments arranged by size:
4678 516 318 126 12
ApaLI G'TGCA_C
Cuts at: 4420 4420
Size: 5650
ApoI r'AATT_y
Cuts at: 2206 2652 5003 5259 2206
Size: 446 2351 256 2597
Fragments arranged by size:
2597 2351 446 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5650
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5650
AvaI C'yCGr_G
Cuts at: 148 293 348 1757 2000 2668 148
Size: 145 55 1409 243 668 3130
Fragments arranged by size:
3130 1409 668 243 145 55
BamHI G'GATC_C
Cuts at: 1320 2673 1320
Size: 1353 4297
BanI G'GyrC_C
Cuts at: 352 468 499 514 628 1132 2615 2664 3078
Size: 116 31 15 114 504 1483 49 414
Cuts at: 3078 3326 3361 352
Size: 248 35 2641
Fragments arranged by size:
2641 1483 504 414 248 116 114 49
35 31 15
BanII G_rGCy'C
Cuts at: 186 570 660 962 974 1184 1334 1490 1616
Size: 384 90 302 12 210 150 156 126
Cuts at: 1616 1934 2662 3692 186
Size: 318 728 1030 2144
Fragments arranged by size:
2144 1030 728 384 318 302 210 156
150 126 90 12
BbeI G_GCGC'C
Cuts at: 472 2619 3082 3330 472
Size: 2147 463 248 2792
Fragments arranged by size:
2792 2147 463 248
BbsI GAAGACnn'nnnn_
Cuts at: 1215 1215
Size: 5650
BbvCI CC'TCA_GC
Cuts at: 267 267
Size: 5650
BciVI GTATCCnnnnn_n'
Cuts at: 2212 3546 4309 5650 2212
Size: 1334 763 1341 2212
Fragments arranged by size:
2212 1341 1334 763
BclI T'GATC_A
Cuts at: 3168 3168
Size: 5650
BfrBI ATG'CAT
Cuts at: 1256 2096 1256
Size: 840 4810
BglI GCCn_nnn'nGGC
Cuts at: 277 1853 277
Size: 1576 4074
BglII A'GATC_T
Cuts at: 3163 5202 3163
Size: 2039 3611
BlpI GC'TnA_GC
Cuts at: 1857 2760 1857
Size: 903 4747
Bme1580I G_kGCm'C
Cuts at: 355 962 974 1135 1353 1391 1490 1616 1934
Size: 607 12 161 218 38 99 126 318
Cuts at: 1934 2007 2534 3273 3366 4424 355
Size: 73 527 739 93 1058 1581
Fragments arranged by size:
1581 1058 739 607 527 318 218 161
126 99 93 73 38 12
BmrI ACTGGGnnnn_n'
Cuts at: 564 968 1222 1950 2017 2860 2941 3276 5422
Size: 404 254 728 67 843 81 335 2146
Cuts at: 5422 564
Size: 792
Fragments arranged by size:
2146 843 792 728 404 335 254 81
67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 438 1301 4015 438
Size: 863 2714 2073
Fragments arranged by size:
2714 2073 863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 595 640 1168 1645 2047 2620 433
Size: 162 45 528 477 402 573 3463
Fragments arranged by size:
3463 573 528 477 402 162 45
Bpu10I CC'TnA_GC
Cuts at: 267 473 267
Size: 206 5444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2824 3703 4197 4495 2824
Size: 879 494 298 3979
Fragments arranged by size:
3979 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 5354 5354
Size: 5650
BsaAI yAC'GTr
Cuts at: 2640 3001 2640
Size: 361 5289
BsaBI GATnn'nnATC
Cuts at: 6 3162 5201 6
Size: 3156 2039 455
Fragments arranged by size:
3156 2039 455
BsaHI Gr'CG_yC
Cuts at: 469 2616 3079 3327 4031 5527 469
Size: 2147 463 248 704 1496 592
Fragments arranged by size:
2147 1496 704 592 463 248
BsaWI w'CCGG_w
Cuts at: 424 531 2291 2837 3358 4038 4312 4459 4870
Size: 107 1760 546 521 680 274 147 411
Cuts at: 4870 424
Size: 1204
Fragments arranged by size:
1760 1204 680 546 521 411 274 147
107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 179 209 295 325 593 623 1321 1351 179
Size: 30 86 30 268 30 698 30 4478
Fragments arranged by size:
4478 698 268 86 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 313 316 710 967 1342 1743 1891 2061 313
Size: 3 394 257 375 401 148 170 3902
Fragments arranged by size:
3902 401 394 375 257 170 148 3
BseYI C'CCAG_C
Cuts at: 480 643 733 1113 1691 2459 3073 3831 4410
Size: 163 90 380 578 768 614 758 579
Cuts at: 4410 4906 480
Size: 496 1224
Fragments arranged by size:
1224 768 758 614 579 578 496 380
163 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 472 535 1749 2563 451
Size: 21 63 1214 814 3538
Fragments arranged by size:
3538 1214 814 63 21
BsiEI CG_ry'CG
Cuts at: 104 2539 3190 3236 4446 104
Size: 2435 651 46 1210 1308
Fragments arranged by size:
2435 1308 1210 651 46
BsiHKAI G_wGCw'C
Cuts at: 1282 1543 2592 2662 3440 3630 4424 1282
Size: 261 1049 70 778 190 794 2508
Fragments arranged by size:
2508 1049 794 778 261 190 70
BsmI GAATG_Cn'
Cuts at: 820 820
Size: 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 388 388
Size: 5650
Bsp1286I G_dGCh'C
Cuts at: 186 355 570 660 962 974 1135 1184 1282
Size: 169 215 90 302 12 161 49 98
Cuts at: 1282 1334 1353 1391 1490 1543 1616 1934 2007
Size: 52 19 38 99 53 73 318 73
Cuts at: 2007 2534 2592 2662 3273 3366 3440 3630 3692
Size: 527 58 70 611 93 74 190 62
Cuts at: 3692 4424 186
Size: 732 1412
Fragments arranged by size:
1412 732 611 527 318 302 215 190
169 161 99 98 93 90 74 73
73 70 62 58 53 52 49 38
19 12
BspEI T'CCGG_A
Cuts at: 531 4870 531
Size: 4339 1311
BspHI T'CATG_A
Cuts at: 1206 4826 1206
Size: 3620 2030
BspMI ACCTGCnnnn'nnnn_
Cuts at: 423 857 1999 2698 2912 3214 3595 423
Size: 434 1142 699 214 302 381 2478
Fragments arranged by size:
2478 1142 699 434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 216 1826 3940 3994 5480 5645 50
Size: 166 1610 2114 54 1486 165 55
Fragments arranged by size:
2114 1610 1486 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1417 3560 1417
Size: 2143 3507
BsrFI r'CCGG_y
Cuts at: 3646 3827 4038 3646
Size: 181 211 5258
Fragments arranged by size:
5258 211 181
BssSI C'ACGA_G
Cuts at: 1767 3919 4279 1767
Size: 2152 360 3138
Fragments arranged by size:
3138 2152 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1607 2156 3025 1607
Size: 549 869 4232
Fragments arranged by size:
4232 869 549
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 1555 2376 1555
Size: 821 4829
BstYI r'GATC_y
Cuts at: 1 528 1320 2603 2673 3140 3163 3498 3744
Size: 527 792 1283 70 467 23 335 246
Cuts at: 3744 4758 4844 4856 5202 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1283 1014 792 527 467 449 346 335
246 86 70 23 12
Bsu36I CC'TnA_GG
Cuts at: 1031 1325 1031
Size: 294 5356
BtgI C'CryG_G
Cuts at: 825 1708 1934 2032 2243 2445 825
Size: 883 226 98 211 202 4030
Fragments arranged by size:
4030 883 226 211 202 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3598 3779 3942 3598
Size: 181 163 5306
Fragments arranged by size:
5306 181 163
BtsI GCAGTG_nn'
Cuts at: 1196 1353 1888 2417 2470 2891 1196
Size: 157 535 529 53 421 3955
Fragments arranged by size:
3955 535 529 421 157 53
DraI TTT'AAA
Cuts at: 2647 2647
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 3354 4214 3354
Size: 860 4790
EaeI y'GGCC_r
Cuts at: 278 815 1545 1557 2286 2536 3233 3407 3798
Size: 537 730 12 729 250 697 174 391
Cuts at: 3798 3825 5172 278
Size: 27 1347 756
Fragments arranged by size:
1347 756 730 729 697 537 391 250
174 27 12
EagI C'GGCC_G
Cuts at: 2536 3233 2536
Size: 697 4953
EarI CTCTTCn'nnn_
Cuts at: 670 744 1918 2580 3671 3881 670
Size: 74 1174 662 1091 210 2439
Fragments arranged by size:
2439 1174 1091 662 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3856 4168 4314 5460 3856
Size: 312 146 1146 4046
Fragments arranged by size:
4046 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2660 2660
Size: 5650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 595 640 1168 1645 2047 2184 2332 2620
Size: 162 45 528 477 402 137 148 288
Cuts at: 2620 3473 3905 4654 4922 433
Size: 853 432 749 268 1161
Fragments arranged by size:
1161 853 749 528 477 432 402 288
268 162 148 137 45
EcoNI CCTnn'n_nnAGG
Cuts at: 463 463
Size: 5650
EcoO109I rG'GnC_Cy
Cuts at: 649 697 958 959 970 1017 1083 1696 1930
Size: 48 261 1 11 47 66 613 234
Cuts at: 1930 2048 2787 649
Size: 118 739 3512
Fragments arranged by size:
3512 739 613 261 234 118 66 48
47 11 1
EcoRI G'AATT_C
Cuts at: 2652 2652
Size: 5650
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3667 3667
Size: 5650
FspI TGC'GCA
Cuts at: 994 3429 994
Size: 2435 3215
HaeII r_GCGC'y
Cuts at: 171 472 762 846 2619 3082 3330 4354 5333
Size: 301 290 84 1773 463 248 1024 979
Cuts at: 5333 171
Size: 488
Fragments arranged by size:
1773 1024 979 488 463 301 290 248
84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 520 552 1321 1353 2056 2088 2422 2454 3490
Size: 32 769 32 703 32 334 32 1036
Cuts at: 3490 3520 3522 3552 520
Size: 30 2 30 2618
Fragments arranged by size:
2618 1036 769 703 334 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2687 2687
Size: 5650
KasI G'GCGC_C
Cuts at: 468 2615 3078 3326 468
Size: 2147 463 248 2792
Fragments arranged by size:
2792 2147 463 248
KpnI G_GTAC'C
Cuts at: 2668 2668
Size: 5650
MluI A'CGCG_T
Cuts at: 5083 5083
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4321 4505 4321
Size: 184 5466
MscI TGG'CCA
Cuts at: 280 1547 1559 2288 3409 280
Size: 1267 12 729 1121 2521
Fragments arranged by size:
2521 1267 1121 729 12
MslI CAynn'nnrTG
Cuts at: 557 1553 1960 2356 2374 3637 3764 557
Size: 996 407 396 18 1263 127 2443
Fragments arranged by size:
2443 1263 996 407 396 127 18
MspA1I CmG'CkG
Cuts at: 689 1014 1476 1512 2245 2411 2759 3073 3433
Size: 325 462 36 733 166 348 314 360
Cuts at: 3433 4448 4693 689
Size: 1015 245 1646
Fragments arranged by size:
1646 1015 733 462 360 348 325 314
245 166 36
NaeI GCC'GGC
Cuts at: 3829 3829
Size: 5650
NarI GG'CG_CC
Cuts at: 469 2616 3079 3327 469
Size: 2147 463 248 2792
Fragments arranged by size:
2792 2147 463 248
NcoI C'CATG_G
Cuts at: 1708 1934 2445 1708
Size: 226 511 4913
Fragments arranged by size:
4913 511 226
NgoMIV G'CCGG_C
Cuts at: 3827 3827
Size: 5650
NruI TCG'CGA
Cuts at: 4011 4011
Size: 5650
NsiI A_TGCA'T
Cuts at: 1258 2098 1258
Size: 840 4810
NspI r_CATG'y
Cuts at: 1233 2701 3201 4110 1233
Size: 1468 500 909 2773
Fragments arranged by size:
2773 1468 909 500
PasI CC'CwG_GG
Cuts at: 1589 1927 2621 1589
Size: 338 694 4618
Fragments arranged by size:
4618 694 338
PciI A'CATG_T
Cuts at: 4106 4106
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 618 1296 1684 1940 618
Size: 678 388 256 4328
Fragments arranged by size:
4328 678 388 256
PfoI T'CCnGG_A
Cuts at: 1370 1661 1370
Size: 291 5359
PmeI GTTT'AAAC
Cuts at: 2647 2647
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 593 625 4789 4821 593
Size: 32 4164 32 1422
Fragments arranged by size:
4164 1422 32 32
PpuMI rG'GwC_Cy
Cuts at: 649 697 1083 1696 2048 649
Size: 48 386 613 352 4251
Fragments arranged by size:
4251 613 386 352 48
PspOMI G'GGCC_C
Cuts at: 958 970 1486 1612 1930 958
Size: 12 516 126 318 4678
Fragments arranged by size:
4678 516 318 126 12
PstI C_TGCA'G
Cuts at: 1348 2424 2695 3380 1348
Size: 1076 271 685 3618
Fragments arranged by size:
3618 1076 685 271
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5650
PvuII CAG'CTG
Cuts at: 1476 1512 3073 3433 1476
Size: 36 1561 360 3693
Fragments arranged by size:
3693 1561 360 36
RsrII CG'GwC_CG
Cuts at: 3843 3843
Size: 5650
SacI G_AGCT'C
Cuts at: 2662 2662
Size: 5650
SacII CC_GC'GG
Cuts at: 2246 2246
Size: 5650
SalI G'TCGA_C
Cuts at: 2685 2685
Size: 5650
SanDI GG'GwC_CC
Cuts at: 2048 2048
Size: 5650
SapI GCTCTTCn'nnn_
Cuts at: 744 2580 3671 3881 744
Size: 1836 1091 210 2513
Fragments arranged by size:
2513 1836 1091 210
SbfI CC_TGCA'GG
Cuts at: 2695 2695
Size: 5650
ScaI AGT'ACT
Cuts at: 1798 5167 1798
Size: 3369 2281
SfcI C'TryA_G
Cuts at: 33 1344 2406 2420 2691 3376 4371 4562 33
Size: 1311 1062 14 271 685 995 191 1121
Fragments arranged by size:
1311 1121 1062 995 685 271 191 14
SfoI GGC'GCC
Cuts at: 470 2617 3080 3328 470
Size: 2147 463 248 2792
Fragments arranged by size:
2792 2147 463 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5650
SmaI CCC'GGG
Cuts at: 150 295 2002 2670 150
Size: 145 1707 668 3130
Fragments arranged by size:
3130 1707 668 145
SmlI C'TyrA_G
Cuts at: 2803 3718 4212 4474 2803
Size: 915 494 262 3979
Fragments arranged by size:
3979 915 494 262
SnaBI TAC'GTA
Cuts at: 2640 2640
Size: 5650
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5650
SphI G_CATG'C
Cuts at: 2701 3201 2701
Size: 500 5150
SrfI GCCC'GGGC
Cuts at: 2002 2002
Size: 5650
SspI AAT'ATT
Cuts at: 1674 1674
Size: 5650
StuI AGG'CCT
Cuts at: 385 880 1624 1773 385
Size: 495 744 149 4262
Fragments arranged by size:
4262 744 495 149
StyI C'CwwG_G
Cuts at: 875 1699 1708 1934 2445 2782 3136 875
Size: 824 9 226 511 337 354 3389
Fragments arranged by size:
3389 824 511 354 337 226 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 905 1621 2064 2269 3231 3364 3823 4034
Size: 433 716 443 205 962 133 459 211
Cuts at: 4034 472
Size: 2088
Fragments arranged by size:
2088 962 716 459 443 433 211 205
133
TatI w'GTAC_w
Cuts at: 1796 5165 1796
Size: 3369 2281
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2462 2494 5167 5199 2462
Size: 32 2673 32 2913
Fragments arranged by size:
2913 2673 32 32
Tth111I GACn'n_nGTC
Cuts at: 2436 3445 2436
Size: 1009 4641
XbaI T'CTAG_A
Cuts at: 2679 2679
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3143 3143
Size: 5650
XmaI C'CCGG_G
Cuts at: 148 293 2000 2668 148
Size: 145 1707 668 3130
Fragments arranged by size:
3130 1707 668 145
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AscI AvrII BaeI BcgI BmgBI
BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI PacI PmlI PshAI PsiI PspXI PsrI
SexAI SfiI SgrAI SwaI XhoI XmnI ZraI