Restriction map for
pF1KSDA0680
(Circular) (Six-base) MAPSORT of: pF1KSDA0680.seq Check: 7083 from: 1 to: 5039
pF1KSDA0680 5039 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1935 1935
Size: 5039
AccI GT'mk_AC
Cuts at: 2001 2077 2001
Size: 76 4963
Acc65I G'GTAC_C
Cuts at: 2055 2055
Size: 5039
AclI AA'CG_TT
Cuts at: 4879 4879
Size: 5039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1274 1379 1652 1727 2864 3296 4043 4311
Size: 774 105 273 75 1137 432 747 268
Cuts at: 4311 500
Size: 1228
Fragments arranged by size:
1228 1137 774 747 432 273 268 105
75
AflII C'TTAA_G
Cuts at: 367 1296 367
Size: 929 4110
AflIII A'CryG_T
Cuts at: 2391 3495 4472 2391
Size: 1104 977 2958
Fragments arranged by size:
2958 1104 977
AgeI A'CCGG_T
Cuts at: 3427 3427
Size: 5039
AleI CACnn'nnGTG
Cuts at: 683 683
Size: 5039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1034 1066 1034
Size: 32 5007
AlwNI CAG_nnn'CTG
Cuts at: 583 1291 1368 2344 3911 583
Size: 708 77 976 1567 1711
Fragments arranged by size:
1711 1567 976 708 77
ApaLI G'TGCA_C
Cuts at: 3809 3809
Size: 5039
ApoI r'AATT_y
Cuts at: 286 414 1081 1963 2043 4392 4648 286
Size: 128 667 882 80 2349 256 677
Fragments arranged by size:
2349 882 677 667 256 128 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 5039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
AvaI C'yCGr_G
Cuts at: 455 776 2059 455
Size: 321 1283 3435
Fragments arranged by size:
3435 1283 321
BamHI G'GATC_C
Cuts at: 1841 2064 1841
Size: 223 4816
BanI G'GyrC_C
Cuts at: 2055 2469 2717 2752 2055
Size: 414 248 35 4342
Fragments arranged by size:
4342 414 248 35
BanII G_rGCy'C
Cuts at: 2053 3083 2053
Size: 1030 4009
BbeI G_GCGC'C
Cuts at: 2473 2721 2473
Size: 248 4791
BbsI GAAGACnn'nnnn_
Cuts at: 280 1357 1502 280
Size: 1077 145 3817
Fragments arranged by size:
3817 1077 145
BbvCI CC'TCA_GC
Cuts at: 1061 1361 1061
Size: 300 4739
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 815 849 815
Size: 34 5005
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2937 3698 5039 1548
Size: 1389 761 1341 1548
Fragments arranged by size:
1548 1389 1341 761
BclI T'GATC_A
Cuts at: 381 2559 381
Size: 2178 2861
BglII A'GATC_T
Cuts at: 2554 4591 2554
Size: 2037 3002
BlpI GC'TnA_GC
Cuts at: 1687 1801 2151 1687
Size: 114 350 4575
Fragments arranged by size:
4575 350 114
Bme1580I G_kGCm'C
Cuts at: 428 2664 2757 3813 428
Size: 2236 93 1056 1654
Fragments arranged by size:
2236 1654 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2251 2332 2667 4811 2251
Size: 81 335 2144 2479
Fragments arranged by size:
2479 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1574 3406 1574
Size: 1832 3207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 541 1076 1100 1166 541
Size: 535 24 66 4414
Fragments arranged by size:
4414 535 66 24
Bpu10I CC'TnA_GC
Cuts at: 608 1061 1361 608
Size: 453 300 4286
Fragments arranged by size:
4286 453 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 2215 3094 3586 3884 1171
Size: 1044 879 492 298 2326
Fragments arranged by size:
2326 1044 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1009 1803 4743 1009
Size: 794 2940 1305
Fragments arranged by size:
2940 1305 794
BsaAI yAC'GTr
Cuts at: 2031 2392 2031
Size: 361 4678
BsaBI GATnn'nnATC
Cuts at: 6 436 2553 4590 6
Size: 430 2117 2037 455
Fragments arranged by size:
2117 2037 455 430
BsaHI Gr'CG_yC
Cuts at: 2470 2718 3420 4916 2470
Size: 248 702 1496 2593
Fragments arranged by size:
2593 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2228 2749 3427 3701 3848 4259 2228
Size: 521 678 274 147 411 3008
Fragments arranged by size:
3008 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 705 735 1863 1893 705
Size: 30 1128 30 3851
Fragments arranged by size:
3851 1128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 593 783 1078 1118 1354 358
Size: 235 190 295 40 236 4043
Fragments arranged by size:
4043 295 236 235 190 40
BseYI C'CCAG_C
Cuts at: 667 1344 2464 3222 3799 4295 667
Size: 677 1120 758 577 496 1411
Fragments arranged by size:
1411 1120 758 677 577 496
BsiEI CG_ry'CG
Cuts at: 104 2581 2627 3835 104
Size: 2477 46 1208 1308
Fragments arranged by size:
2477 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2053 2831 3021 3813 2053
Size: 778 190 792 3279
Fragments arranged by size:
3279 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1224 1224
Size: 5039
Bsp1286I G_dGCh'C
Cuts at: 428 2053 2664 2757 2831 3021 3083 3813 428
Size: 1625 611 93 74 190 62 730 1654
Fragments arranged by size:
1654 1625 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4259 4259
Size: 5039
BspHI T'CATG_A
Cuts at: 1989 4215 1989
Size: 2226 2813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 178 1220 1935 2089 2303 2605 2986 178
Size: 1042 715 154 214 302 381 2231
Fragments arranged by size:
2231 1042 715 381 302 214 154
BsrBI CCG'CTC
Cuts at: 50 3331 3385 4869 5034 50
Size: 3281 54 1484 165 55
Fragments arranged by size:
3281 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2951 2951
Size: 5039
BsrFI r'CCGG_y
Cuts at: 573 3037 3218 3427 573
Size: 2464 181 209 2185
Fragments arranged by size:
2464 2185 209 181
BsrGI T'GTAC_A
Cuts at: 1475 1475
Size: 5039
BssSI C'ACGA_G
Cuts at: 3310 3668 3310
Size: 358 4681
BstAPI GCAn_nnn'nTGC
Cuts at: 583 2416 583
Size: 1833 3206
BstBI TT'CG_AA
Cuts at: 110 3400 110
Size: 3290 1749
BstYI r'GATC_y
Cuts at: 1 616 1841 2064 2531 2554 2889 3135 4147
Size: 615 1225 223 467 23 335 246 1012
Cuts at: 4147 4233 4245 4591 1
Size: 86 12 346 449
Fragments arranged by size:
1225 1012 615 467 449 346 335 246
223 86 23 12
BtgI C'CryG_G
Cuts at: 125 1091 125
Size: 966 4073
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2989 3170 3333 2989
Size: 181 163 4695
Fragments arranged by size:
4695 181 163
BtsI GCAGTG_nn'
Cuts at: 662 1521 2282 662
Size: 859 761 3419
Fragments arranged by size:
3419 859 761
DraI TTT'AAA
Cuts at: 1968 2038 1968
Size: 70 4969
DrdI GACnn_nn'nnGTC
Cuts at: 1216 2745 3603 1216
Size: 1529 858 2652
Fragments arranged by size:
2652 1529 858
EaeI y'GGCC_r
Cuts at: 2624 2798 3189 3216 4561 2624
Size: 174 391 27 1345 3102
Fragments arranged by size:
3102 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2624 2624
Size: 5039
EarI CTCTTCn'nnn_
Cuts at: 1250 1631 1745 1988 3062 3272 1250
Size: 381 114 243 1074 210 3017
Fragments arranged by size:
3017 1074 381 243 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3247 3557 3703 4849 3247
Size: 310 146 1146 3437
Fragments arranged by size:
3437 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2051 2051
Size: 5039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 541 1076 1100 1166 1274 1379 1652 1727
Size: 41 535 24 66 108 105 273 75
Cuts at: 1727 2864 3296 4043 4311 500
Size: 1137 432 747 268 1228
Fragments arranged by size:
1228 1137 747 535 432 273 268 108
105 75 66 41 24
EcoNI CCTnn'n_nnAGG
Cuts at: 653 1914 653
Size: 1261 3778
EcoO109I rG'GnC_Cy
Cuts at: 735 2178 735
Size: 1443 3596
EcoRI G'AATT_C
Cuts at: 2043 2043
Size: 5039
EcoRV GAT'ATC
Cuts at: 323 323
Size: 5039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 5039
FspI TGC'GCA
Cuts at: 2820 2820
Size: 5039
HaeII r_GCGC'y
Cuts at: 2473 2721 3743 4722 2473
Size: 248 1022 979 2790
Fragments arranged by size:
2790 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1344 1376 1441 1473 2881 2911 2913 2943 1344
Size: 32 65 32 1408 30 2 30 3440
Fragments arranged by size:
3440 1408 65 32 32 30 30 2
HincII GTy'rAC
Cuts at: 141 405 1305 1922 2002 2078 141
Size: 264 900 617 80 76 3102
Fragments arranged by size:
3102 900 617 264 80 76
HindIII A'AGCT_T
Cuts at: 155 869 155
Size: 714 4325
HpaI GTT'AAC
Cuts at: 405 1305 1922 405
Size: 900 617 3522
Fragments arranged by size:
3522 900 617
KasI G'GCGC_C
Cuts at: 2469 2717 2469
Size: 248 4791
KpnI G_GTAC'C
Cuts at: 2059 2059
Size: 5039
MluI A'CGCG_T
Cuts at: 4472 4472
Size: 5039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1016 1182 1677 3710 3894 1016
Size: 166 495 2033 184 2161
Fragments arranged by size:
2161 2033 495 184 166
MscI TGG'CCA
Cuts at: 2800 2800
Size: 5039
MslI CAynn'nnrTG
Cuts at: 683 1978 3028 3155 683
Size: 1295 1050 127 2567
Fragments arranged by size:
2567 1295 1050 127
MspA1I CmG'CkG
Cuts at: 2150 2464 2824 3837 4082 2150
Size: 314 360 1013 245 3107
Fragments arranged by size:
3107 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3220 3220
Size: 5039
NarI GG'CG_CC
Cuts at: 2470 2718 2470
Size: 248 4791
NcoI C'CATG_G
Cuts at: 125 1091 125
Size: 966 4073
NgoMIV G'CCGG_C
Cuts at: 3218 3218
Size: 5039
NspI r_CATG'y
Cuts at: 2092 2592 3499 2092
Size: 500 907 3632
Fragments arranged by size:
3632 907 500
PasI CC'CwG_GG
Cuts at: 1910 1910
Size: 5039
PciI A'CATG_T
Cuts at: 3495 3495
Size: 5039
PmeI GTTT'AAAC
Cuts at: 2038 2038
Size: 5039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 5007
PshAI GACnn'nnGTC
Cuts at: 1235 1235
Size: 5039
PstI C_TGCA'G
Cuts at: 1014 1932 2086 2771 1014
Size: 918 154 685 3282
Fragments arranged by size:
3282 918 685 154
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5039
PvuII CAG'CTG
Cuts at: 2464 2824 2464
Size: 360 4679
RsrII CG'GwC_CG
Cuts at: 3234 3234
Size: 5039
SacI G_AGCT'C
Cuts at: 2053 2053
Size: 5039
SalI G'TCGA_C
Cuts at: 2000 2076 2000
Size: 76 4963
SapI GCTCTTCn'nnn_
Cuts at: 1250 3062 3272 1250
Size: 1812 210 3017
Fragments arranged by size:
3017 1812 210
SbfI CC_TGCA'GG
Cuts at: 2086 2086
Size: 5039
ScaI AGT'ACT
Cuts at: 4556 4556
Size: 5039
SfcI C'TryA_G
Cuts at: 33 161 1010 1928 2082 2767 3760 3951 33
Size: 128 849 918 154 685 993 191 1121
Fragments arranged by size:
1121 993 918 849 685 191 154 128
SfoI GGC'GCC
Cuts at: 2471 2719 2471
Size: 248 4791
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
SmaI CCC'GGG
Cuts at: 2061 2061
Size: 5039
SmlI C'TyrA_G
Cuts at: 367 1150 1296 2194 3109 3601 3863 367
Size: 783 146 898 915 492 262 1543
Fragments arranged by size:
1543 915 898 783 492 262 146
SnaBI TAC'GTA
Cuts at: 2031 2031
Size: 5039
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5039
SphI G_CATG'C
Cuts at: 2092 2592 2092
Size: 500 4539
StyI C'CwwG_G
Cuts at: 125 253 716 917 1091 2173 2527 125
Size: 128 463 201 174 1082 354 2637
Fragments arranged by size:
2637 1082 463 354 201 174 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 765 2622 2755 3214 3423 765
Size: 1857 133 459 209 2381
Fragments arranged by size:
2381 1857 459 209 133
TatI w'GTAC_w
Cuts at: 1475 4554 1475
Size: 3079 1960
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1863 1895 4556 4588 1863
Size: 32 2661 32 2314
Fragments arranged by size:
2661 2314 32 32
Tth111I GACn'n_nGTC
Cuts at: 2836 2836
Size: 5039
XbaI T'CTAG_A
Cuts at: 2070 2070
Size: 5039
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1341 2534 1341
Size: 1193 3846
XmaI C'CCGG_G
Cuts at: 2059 2059
Size: 5039
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsgI BsiWI BsmI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII FseI
FspAI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SrfI SspI StuI
SwaI XhoI XmnI ZraI