Restriction map for
pF1KSDA0679
(Circular) (Six-base) MAPSORT of: pF1KSDA0679.seq Check: 4693 from: 1 to: 5887
pF1KSDA0679 5887 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 2539 2923 2539
Size: 384 5503
Acc65I G'GTAC_C
Cuts at: 2901 2901
Size: 5887
AclI AA'CG_TT
Cuts at: 5727 5727
Size: 5887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 2749 3710 4142 4891 5159 2111
Size: 638 961 432 749 268 2839
Fragments arranged by size:
2839 961 749 638 432 268
AflIII A'CryG_T
Cuts at: 2042 3237 4343 5320 2042
Size: 1195 1106 977 2609
Fragments arranged by size:
2609 1195 1106 977
AgeI A'CCGG_T
Cuts at: 4275 4275
Size: 5887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1823 1823
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 3190 4759 3190
Size: 1569 4318
ApaI G_GGCC'C
Cuts at: 1009 1009
Size: 5887
ApaLI G'TGCA_C
Cuts at: 4657 4657
Size: 5887
ApoI r'AATT_y
Cuts at: 899 1075 1867 2507 2783 2791 2889 5240 5496
Size: 176 792 640 276 8 98 2351 256
Cuts at: 5496 899
Size: 1290
Fragments arranged by size:
2351 1290 792 640 276 256 176 98
8
AseI AT'TA_AT
Cuts at: 20 20
Size: 5887
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5887
AvaI C'yCGr_G
Cuts at: 2744 2905 2744
Size: 161 5726
AvrII C'CTAG_G
Cuts at: 741 741
Size: 5887
BamHI G'GATC_C
Cuts at: 2516 2910 2516
Size: 394 5493
BanI G'GyrC_C
Cuts at: 231 2901 3315 3563 3598 231
Size: 2670 414 248 35 2520
Fragments arranged by size:
2670 2520 414 248 35
BanII G_rGCy'C
Cuts at: 176 297 948 1009 1433 2899 3929 176
Size: 121 651 61 424 1466 1030 2134
Fragments arranged by size:
2134 1466 1030 651 424 121 61
BbeI G_GCGC'C
Cuts at: 3319 3567 3319
Size: 248 5639
BbsI GAAGACnn'nnnn_
Cuts at: 248 342 2714 248
Size: 94 2372 3421
Fragments arranged by size:
3421 2372 94
BbvCI CC'TCA_GC
Cuts at: 2410 2410
Size: 5887
BciVI GTATCCnnnnn_n'
Cuts at: 588 3783 4546 5887 588
Size: 3195 763 1341 588
Fragments arranged by size:
3195 1341 763 588
BclI T'GATC_A
Cuts at: 984 3405 984
Size: 2421 3466
BglII A'GATC_T
Cuts at: 3400 5439 3400
Size: 2039 3848
BlpI GC'TnA_GC
Cuts at: 175 473 2997 175
Size: 298 2524 3065
Fragments arranged by size:
3065 2524 298
Bme1580I G_kGCm'C
Cuts at: 1009 2473 3510 3603 4661 1009
Size: 1464 1037 93 1058 2235
Fragments arranged by size:
2235 1464 1058 1037 93
BmrI ACTGGGnnnn_n'
Cuts at: 3097 3178 3513 5659 3097
Size: 81 335 2146 3325
Fragments arranged by size:
3325 2146 335 81
BmtI G_CTAG'C
Cuts at: 1273 1273
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 4252 195
Size: 4057 1830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 364 932 1810 232
Size: 132 568 878 4309
Fragments arranged by size:
4309 878 568 132
Bpu10I CC'TnA_GC
Cuts at: 2410 2410
Size: 5887
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1476 3061 3940 4434 4732 1476
Size: 1585 879 494 298 2631
Fragments arranged by size:
2631 1585 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2141 2760 5591 2141
Size: 619 2831 2437
Fragments arranged by size:
2831 2437 619
BsaAI yAC'GTr
Cuts at: 2877 3238 2877
Size: 361 5526
BsaBI GATnn'nnATC
Cuts at: 6 983 3399 5438 6
Size: 977 2416 2039 455
Fragments arranged by size:
2416 2039 977 455
BsaHI Gr'CG_yC
Cuts at: 3316 3564 4268 5764 3316
Size: 248 704 1496 3439
Fragments arranged by size:
3439 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3074 3595 4275 4549 4696 5107 3074
Size: 521 680 274 147 411 3854
Fragments arranged by size:
3854 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 257 287 2032 2062 257
Size: 30 1745 30 4082
Fragments arranged by size:
4082 1745 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 172 172
Size: 5887
BseYI C'CCAG_C
Cuts at: 259 3310 4068 4647 5143 259
Size: 3051 758 579 496 1003
Fragments arranged by size:
3051 1003 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 485 642 689 151
Size: 334 157 47 5349
Fragments arranged by size:
5349 334 157 47
BsiEI CG_ry'CG
Cuts at: 104 3427 3473 4683 104
Size: 3323 46 1210 1308
Fragments arranged by size:
3323 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 176 948 1854 2899 3677 3867 4661 176
Size: 772 906 1045 778 190 794 1402
Fragments arranged by size:
1402 1045 906 794 778 772 190
Bsp1286I G_dGCh'C
Cuts at: 176 297 948 1009 1433 1854 2473 2899 3510
Size: 121 651 61 424 421 619 426 611
Cuts at: 3510 3603 3677 3867 3929 4661 176
Size: 93 74 190 62 732 1402
Fragments arranged by size:
1402 732 651 619 611 426 424 421
190 121 93 74 62 61
BspEI T'CCGG_A
Cuts at: 5107 5107
Size: 5887
BspHI T'CATG_A
Cuts at: 5063 5063
Size: 5887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2935 3149 3451 3832 2935
Size: 214 302 381 4990
Fragments arranged by size:
4990 381 302 214
BsrBI CCG'CTC
Cuts at: 50 162 268 4177 4231 5717 5882 50
Size: 112 106 3909 54 1486 165 55
Fragments arranged by size:
3909 1486 165 112 106 55 54
BsrDI GCAATG_nn'
Cuts at: 1694 3797 1694
Size: 2103 3784
BsrFI r'CCGG_y
Cuts at: 219 3883 4064 4275 219
Size: 3664 181 211 1831
Fragments arranged by size:
3664 1831 211 181
BsrGI T'GTAC_A
Cuts at: 1766 2097 2309 1766
Size: 331 212 5344
Fragments arranged by size:
5344 331 212
BssSI C'ACGA_G
Cuts at: 503 1952 4156 4516 503
Size: 1449 2204 360 1874
Fragments arranged by size:
2204 1874 1449 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1740 3262 1740
Size: 1522 4365
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5887
BstXI CCAn_nnnn'nTGG
Cuts at: 956 1524 956
Size: 568 5319
BstYI r'GATC_y
Cuts at: 1 1021 2516 2910 3377 3400 3735 3981 4995
Size: 1020 1495 394 467 23 335 246 1014
Cuts at: 4995 5081 5093 5439 1
Size: 86 12 346 449
Fragments arranged by size:
1495 1020 1014 467 449 394 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 2540 2540
Size: 5887
Bsu36I CC'TnA_GG
Cuts at: 2291 2291
Size: 5887
BtgI C'CryG_G
Cuts at: 125 1039 1517 125
Size: 914 478 4495
Fragments arranged by size:
4495 914 478
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 191 3835 4016 4179 191
Size: 3644 181 163 1899
Fragments arranged by size:
3644 1899 181 163
BtsI GCAGTG_nn'
Cuts at: 1221 1769 3128 1221
Size: 548 1359 3980
Fragments arranged by size:
3980 1359 548
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2831 2866 2831
Size: 35 5852
DraI TTT'AAA
Cuts at: 1394 2884 1394
Size: 1490 4397
DraIII CAC_nnn'GTG
Cuts at: 1339 1339
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 3591 4451 3591
Size: 860 5027
EaeI y'GGCC_r
Cuts at: 275 2693 3470 3644 4035 4062 5409 275
Size: 2418 777 174 391 27 1347 753
Fragments arranged by size:
2418 1347 777 753 391 174 27
EagI C'GGCC_G
Cuts at: 3470 3470
Size: 5887
EarI CTCTTCn'nnn_
Cuts at: 769 914 985 1964 3908 4118 769
Size: 145 71 979 1944 210 2538
Fragments arranged by size:
2538 1944 979 210 145 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4093 4405 4551 5697 4093
Size: 312 146 1146 4283
Fragments arranged by size:
4283 1146 312 146
EcoICRI GAG'CTC
Cuts at: 174 946 2897 174
Size: 772 1951 3164
Fragments arranged by size:
3164 1951 772
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 364 932 1810 2111 2749 3710 4142 4891
Size: 132 568 878 301 638 961 432 749
Cuts at: 4891 5159 232
Size: 268 960
Fragments arranged by size:
961 960 878 749 638 568 432 301
268 132
EcoO109I rG'GnC_Cy
Cuts at: 877 3024 877
Size: 2147 3740
EcoRI G'AATT_C
Cuts at: 2783 2889 2783
Size: 106 5781
EcoRV GAT'ATC
Cuts at: 552 2064 552
Size: 1512 4375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3904 3904
Size: 5887
FspI TGC'GCA
Cuts at: 3666 3666
Size: 5887
HaeII r_GCGC'y
Cuts at: 3319 3567 4591 5570 3319
Size: 248 1024 979 3636
Fragments arranged by size:
3636 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 536 568 2032 2064 2287 2319 3727 3757 3759
Size: 32 1464 32 223 32 1408 30 2
Cuts at: 3759 3789 536
Size: 30 2634
Fragments arranged by size:
2634 1464 1408 223 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2924 2924
Size: 5887
HindIII A'AGCT_T
Cuts at: 844 844
Size: 5887
KasI G'GCGC_C
Cuts at: 3315 3563 3315
Size: 248 5639
KpnI G_GTAC'C
Cuts at: 2905 2905
Size: 5887
MfeI C'AATT_G
Cuts at: 1908 2013 2207 1908
Size: 105 194 5588
Fragments arranged by size:
5588 194 105
MluI A'CGCG_T
Cuts at: 5320 5320
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 134 206 2845 4558 4742 134
Size: 72 2639 1713 184 1279
Fragments arranged by size:
2639 1713 1279 184 72
MscI TGG'CCA
Cuts at: 2695 3646 2695
Size: 951 4936
MslI CAynn'nnrTG
Cuts at: 974 1522 1528 1559 3874 4001 974
Size: 548 6 31 2315 127 2860
Fragments arranged by size:
2860 2315 548 127 31 6
MspA1I CmG'CkG
Cuts at: 2996 3310 3670 4685 4930 2996
Size: 314 360 1015 245 3953
Fragments arranged by size:
3953 1015 360 314 245
NaeI GCC'GGC
Cuts at: 221 4066 221
Size: 3845 2042
NarI GG'CG_CC
Cuts at: 3316 3564 3316
Size: 248 5639
NcoI C'CATG_G
Cuts at: 125 1517 125
Size: 1392 4495
NdeI CA'TA_TG
Cuts at: 2452 2452
Size: 5887
NgoMIV G'CCGG_C
Cuts at: 219 4064 219
Size: 3845 2042
NheI G'CTAG_C
Cuts at: 1269 1269
Size: 5887
NruI TCG'CGA
Cuts at: 4248 4248
Size: 5887
NspI r_CATG'y
Cuts at: 2046 2938 3438 4347 2046
Size: 892 500 909 3586
Fragments arranged by size:
3586 909 892 500
PasI CC'CwG_GG
Cuts at: 2159 2159
Size: 5887
PciI A'CATG_T
Cuts at: 2042 4343 2042
Size: 2301 3586
PflMI CCAn_nnn'nTGG
Cuts at: 2212 2212
Size: 5887
PfoI T'CCnGG_A
Cuts at: 2512 2512
Size: 5887
PmeI GTTT'AAAC
Cuts at: 2884 2884
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 505 537 5026 5058 505
Size: 32 4489 32 1334
Fragments arranged by size:
4489 1334 32 32
PpuMI rG'GwC_Cy
Cuts at: 877 877
Size: 5887
PspOMI G'GGCC_C
Cuts at: 1005 1005
Size: 5887
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 5855
PstI C_TGCA'G
Cuts at: 288 2932 3617 288
Size: 2644 685 2558
Fragments arranged by size:
2644 2558 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5887
PvuII CAG'CTG
Cuts at: 3310 3670 3310
Size: 360 5527
RsrII CG'GwC_CG
Cuts at: 4080 4080
Size: 5887
SacI G_AGCT'C
Cuts at: 176 948 2899 176
Size: 772 1951 3164
Fragments arranged by size:
3164 1951 772
SalI G'TCGA_C
Cuts at: 2922 2922
Size: 5887
SapI GCTCTTCn'nnn_
Cuts at: 3908 4118 3908
Size: 210 5677
SbfI CC_TGCA'GG
Cuts at: 2932 2932
Size: 5887
ScaI AGT'ACT
Cuts at: 1179 2615 5404 1179
Size: 1436 2789 1662
Fragments arranged by size:
2789 1662 1436
SexAI A'CCwGG_T
Cuts at: 602 1114 602
Size: 512 5375
SfcI C'TryA_G
Cuts at: 33 284 1245 2474 2928 3613 4608 4799 33
Size: 251 961 1229 454 685 995 191 1121
Fragments arranged by size:
1229 1121 995 961 685 454 251 191
SfoI GGC'GCC
Cuts at: 3317 3565 3317
Size: 248 5639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5887
SmaI CCC'GGG
Cuts at: 2746 2907 2746
Size: 161 5726
SmlI C'TyrA_G
Cuts at: 1455 3040 3955 4449 4711 1455
Size: 1585 915 494 262 2631
Fragments arranged by size:
2631 1585 915 494 262
SnaBI TAC'GTA
Cuts at: 2877 2877
Size: 5887
SpeI A'CTAG_T
Cuts at: 67 1983 67
Size: 1916 3971
SphI G_CATG'C
Cuts at: 2938 3438 2938
Size: 500 5387
StyI C'CwwG_G
Cuts at: 125 340 569 741 1481 1517 2465 3019 3373
Size: 215 229 172 740 36 948 554 354
Cuts at: 3373 125
Size: 2639
Fragments arranged by size:
2639 948 740 554 354 229 215 172
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 608 826 3468 3601 4060 4271 608
Size: 218 2642 133 459 211 2224
Fragments arranged by size:
2642 2224 459 218 211 133
TatI w'GTAC_w
Cuts at: 1177 1239 1766 2045 2097 2309 2613 5402 1177
Size: 62 527 279 52 212 304 2789 1662
Fragments arranged by size:
2789 1662 527 304 279 212 62 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 257 289 822 848 854 880 5404 5436 257
Size: 32 533 26 6 26 4524 32 708
Fragments arranged by size:
4524 708 533 32 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 3682 3682
Size: 5887
XbaI T'CTAG_A
Cuts at: 2916 2916
Size: 5887
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1524 2703 3380 1524
Size: 1179 677 4031
Fragments arranged by size:
4031 1179 677
XmaI C'CCGG_G
Cuts at: 2744 2905 2744
Size: 161 5726
XmnI GAAnn'nnTTC
Cuts at: 845 2837 845
Size: 1992 3895
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
BaeI BcgI BfrBI BglI BmgBI BsiWI BsmI BsmBI
BssHII BstEII ClaI EcoNI FseI FspAI HpaI NotI
NsiI PacI PmlI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SrfI SspI StuI SwaI XhoI ZraI