Restriction map for
pF1KSDA0655
(Circular) (Six-base) MAPSORT of: pF1KSDA0655.seq Check: 1229 from: 1 to: 6343
pF1KSDA0655 6343 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1608 2075 2305 3066 1608
Size: 467 230 761 4885
Fragments arranged by size:
4885 761 467 230
AccI GT'mk_AC
Cuts at: 521 2474 3379 521
Size: 1953 905 3485
Fragments arranged by size:
3485 1953 905
Acc65I G'GTAC_C
Cuts at: 446 3357 446
Size: 2911 3432
AclI AA'CG_TT
Cuts at: 6183 6183
Size: 6343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1297 1495 2392 3073 4166 4598 5347 5615
Size: 591 198 897 681 1093 432 749 268
Cuts at: 5615 706
Size: 1434
Fragments arranged by size:
1434 1093 897 749 681 591 432 268
198
AflIII A'CryG_T
Cuts at: 3693 4799 5776 3693
Size: 1106 977 4260
Fragments arranged by size:
4260 1106 977
AgeI A'CCGG_T
Cuts at: 878 4731 878
Size: 3853 2490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1969 2001 1969
Size: 32 6311
AlwNI CAG_nnn'CTG
Cuts at: 1419 1851 1959 1971 2160 2358 2424 3646 5215
Size: 432 108 12 189 198 66 1222 1569
Cuts at: 5215 1419
Size: 2547
Fragments arranged by size:
2547 1569 1222 432 198 189 108 66
12
ApaI G_GGCC'C
Cuts at: 1100 2109 2316 1100
Size: 1009 207 5127
Fragments arranged by size:
5127 1009 207
ApaLI G'TGCA_C
Cuts at: 1504 5113 1504
Size: 3609 2734
ApoI r'AATT_y
Cuts at: 2704 3345 5696 5952 2704
Size: 641 2351 256 3095
Fragments arranged by size:
3095 2351 641 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6343
AvaI C'yCGr_G
Cuts at: 973 1042 2315 2562 3361 973
Size: 69 1273 247 799 3955
Fragments arranged by size:
3955 1273 799 247 69
BamHI G'GATC_C
Cuts at: 2591 3366 2591
Size: 775 5568
BanI G'GyrC_C
Cuts at: 416 446 2245 3357 3771 4019 4054 416
Size: 30 1799 1112 414 248 35 2705
Fragments arranged by size:
2705 1799 1112 414 248 35 30
BanII G_rGCy'C
Cuts at: 1100 1496 1765 2109 2316 2322 2330 2834 3355
Size: 396 269 344 207 6 8 504 521
Cuts at: 3355 4385 1100
Size: 1030 3058
Fragments arranged by size:
3058 1030 521 504 396 344 269 207
8 6
BbeI G_GCGC'C
Cuts at: 3775 4023 3775
Size: 248 6095
BbsI GAAGACnn'nnnn_
Cuts at: 3106 3106
Size: 6343
BbvCI CC'TCA_GC
Cuts at: 1002 1381 1002
Size: 379 5964
BciVI GTATCCnnnnn_n'
Cuts at: 744 2220 4239 5002 6343 744
Size: 1476 2019 763 1341 744
Fragments arranged by size:
2019 1476 1341 763 744
BclI T'GATC_A
Cuts at: 3861 3861
Size: 6343
BglI GCCn_nnn'nGGC
Cuts at: 1456 2113 2191 2755 2866 1456
Size: 657 78 564 111 4933
Fragments arranged by size:
4933 657 564 111 78
BglII A'GATC_T
Cuts at: 545 3856 5895 545
Size: 3311 2039 993
Fragments arranged by size:
3311 2039 993
BlpI GC'TnA_GC
Cuts at: 160 1013 1759 1876 3453 160
Size: 853 746 117 1577 3050
Fragments arranged by size:
3050 1577 853 746 117
Bme1580I G_kGCm'C
Cuts at: 298 419 1100 1508 2109 2316 3966 4059 5117
Size: 121 681 408 601 207 1650 93 1058
Cuts at: 5117 298
Size: 1524
Fragments arranged by size:
1650 1524 1058 681 601 408 207 121
93
BmgBI CAC'GTC
Cuts at: 390 390
Size: 6343
BmrI ACTGGGnnnn_n'
Cuts at: 564 2148 3553 3634 3969 6115 564
Size: 1584 1405 81 335 2146 792
Fragments arranged by size:
2146 1584 1405 792 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1779 1961 4708 1779
Size: 182 2747 3414
Fragments arranged by size:
3414 2747 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 607 628 1171 1255 1270 1318 1330 1417
Size: 402 21 543 84 15 48 12 87
Cuts at: 1417 1687 1708 2158 2344 2596 3109 3115 3127
Size: 270 21 450 186 252 513 6 12
Cuts at: 3127 3200 205
Size: 73 3348
Fragments arranged by size:
3348 543 513 450 402 270 252 186
87 84 73 48 21 21 15 12
12 6
Bpu10I CC'TnA_GC
Cuts at: 1002 1381 1729 2920 1002
Size: 379 348 1191 4425
Fragments arranged by size:
4425 1191 379 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 1660 3517 4396 4890 5188 544
Size: 1116 1857 879 494 298 1699
Fragments arranged by size:
1857 1699 1116 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 467 6047 467
Size: 5580 763
BsaAI yAC'GTr
Cuts at: 3153 3333 3694 3153
Size: 180 361 5802
Fragments arranged by size:
5802 361 180
BsaBI GATnn'nnATC
Cuts at: 6 3855 5894 6
Size: 3849 2039 455
Fragments arranged by size:
3849 2039 455
BsaHI Gr'CG_yC
Cuts at: 2177 3772 4020 4724 6220 2177
Size: 1595 248 704 1496 2300
Fragments arranged by size:
2300 1595 1496 704 248
BsaWI w'CCGG_w
Cuts at: 878 3530 4051 4731 5005 5152 5563 878
Size: 2652 521 680 274 147 411 1658
Fragments arranged by size:
2652 1658 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1621 1651 2149 2179 3173 3203 1621
Size: 30 498 30 994 30 4761
Fragments arranged by size:
4761 994 498 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1060 1078 1327 1441 1588 1663 2155 2470 2842
Size: 18 249 114 147 75 492 315 372
Cuts at: 2842 3199 1060
Size: 357 4204
Fragments arranged by size:
4204 492 372 357 315 249 147 114
75 18
BseYI C'CCAG_C
Cuts at: 355 619 1256 1376 1391 2412 2460 2765 2870
Size: 264 637 120 15 1021 48 305 105
Cuts at: 2870 3126 3160 3202 3256 3309 3766 4524 5103
Size: 256 34 42 54 53 457 758 579
Cuts at: 5103 5599 355
Size: 496 1099
Fragments arranged by size:
1099 1021 758 637 579 496 457 305
264 256 120 105 54 53 48 42
34 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2047 2589 2047
Size: 542 5801
BsiEI CG_ry'CG
Cuts at: 104 2283 3883 3929 5139 104
Size: 2179 1600 46 1210 1308
Fragments arranged by size:
2179 1600 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1019 1496 1508 1765 2134 2330 2834 3355 4133
Size: 477 12 257 369 196 504 521 778
Cuts at: 4133 4323 5117 1019
Size: 190 794 2245
Fragments arranged by size:
2245 794 778 521 504 477 369 257
196 190 12
BsmI GAATG_Cn'
Cuts at: 1296 1296
Size: 6343
BsmBI CGTCTCn'nnnn_
Cuts at: 404 3064 404
Size: 2660 3683
Bsp1286I G_dGCh'C
Cuts at: 298 419 1019 1100 1496 1508 1765 2109 2134
Size: 121 600 81 396 12 257 344 25
Cuts at: 2134 2316 2322 2330 2834 3355 3966 4059 4133
Size: 182 6 8 504 521 611 93 74
Cuts at: 4133 4323 4385 5117 298
Size: 190 62 732 1524
Fragments arranged by size:
1524 732 611 600 521 504 396 344
257 190 182 121 93 81 74 62
25 12 8 6
BspEI T'CCGG_A
Cuts at: 5563 5563
Size: 6343
BspHI T'CATG_A
Cuts at: 5519 5519
Size: 6343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1608 1841 2075 2305 3066 3391 3605 3907 4288
Size: 233 234 230 761 325 214 302 381
Cuts at: 4288 1608
Size: 3663
Fragments arranged by size:
3663 761 381 325 302 234 233 230
214
BsrBI CCG'CTC
Cuts at: 50 1226 1407 2678 4633 4687 6173 6338 50
Size: 1176 181 1271 1955 54 1486 165 55
Fragments arranged by size:
1955 1486 1271 1176 181 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4253 4253
Size: 6343
BsrFI r'CCGG_y
Cuts at: 147 878 1026 1925 4339 4520 4731 147
Size: 731 148 899 2414 181 211 1759
Fragments arranged by size:
2414 1759 899 731 211 181 148
BssHII G'CGCG_C
Cuts at: 1459 1715 1459
Size: 256 6087
BssSI C'ACGA_G
Cuts at: 304 886 1366 2848 3319 4612 4972 304
Size: 582 480 1482 471 1293 360 1675
Fragments arranged by size:
1675 1482 1293 582 480 471 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1959 1971 2306 2358 3718 1959
Size: 12 335 52 1360 4584
Fragments arranged by size:
4584 1360 335 52 12
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6343
BstXI CCAn_nnnn'nTGG
Cuts at: 2760 2781 2973 2760
Size: 21 192 6130
Fragments arranged by size:
6130 192 21
BstYI r'GATC_y
Cuts at: 1 545 959 2591 3366 3833 3856 4191 4437
Size: 544 414 1632 775 467 23 335 246
Cuts at: 4437 5451 5537 5549 5895 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1632 1014 775 544 467 449 414 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 747 904 747
Size: 157 6186
BtgI C'CryG_G
Cuts at: 578 1103 1451 2018 2468 3020 578
Size: 525 348 567 450 552 3901
Fragments arranged by size:
3901 567 552 525 450 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 711 1251 4291 4472 4635 711
Size: 540 3040 181 163 2419
Fragments arranged by size:
3040 2419 540 181 163
BtsI GCAGTG_nn'
Cuts at: 1997 3206 3584 1997
Size: 1209 378 4756
Fragments arranged by size:
4756 1209 378
DraI TTT'AAA
Cuts at: 3340 3340
Size: 6343
DraIII CAC_nnn'GTG
Cuts at: 3153 3153
Size: 6343
DrdI GACnn_nn'nnGTC
Cuts at: 4047 4907 4047
Size: 860 5483
EaeI y'GGCC_r
Cuts at: 722 2489 3191 3926 4100 4491 4518 5865 722
Size: 1767 702 735 174 391 27 1347 1200
Fragments arranged by size:
1767 1347 1200 735 702 391 174 27
EagI C'GGCC_G
Cuts at: 3926 3926
Size: 6343
EarI CTCTTCn'nnn_
Cuts at: 1138 1198 1273 1648 4364 4574 1138
Size: 60 75 375 2716 210 2907
Fragments arranged by size:
2907 2716 375 210 75 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1351 2172 4549 4861 5007 6153 1351
Size: 821 2377 312 146 1146 1541
Fragments arranged by size:
2377 1541 1146 821 312 146
EcoICRI GAG'CTC
Cuts at: 1494 1763 2328 2832 3353 1494
Size: 269 565 504 521 4484
Fragments arranged by size:
4484 565 521 504 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 607 628 706 1171 1255 1270 1297 1318
Size: 402 21 78 465 84 15 27 21
Cuts at: 1318 1330 1417 1495 1687 1708 2158 2344 2392
Size: 12 87 78 192 21 450 186 48
Cuts at: 2392 2596 3073 3109 3115 3127 3200 4166 4598
Size: 204 477 36 6 12 73 966 432
Cuts at: 4598 5347 5615 205
Size: 749 268 933
Fragments arranged by size:
966 933 749 477 465 450 432 402
268 204 192 186 87 84 78 78
73 48 36 27 21 21 21 15
12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 397 775 1423 2140 2617 2659 397
Size: 378 648 717 477 42 4081
Fragments arranged by size:
4081 717 648 477 378 42
EcoO109I rG'GnC_Cy
Cuts at: 255 402 979 1050 1096 1097 1182 1338 1416
Size: 147 577 71 46 1 85 156 78
Cuts at: 1416 1725 2105 2312 3480 255
Size: 309 380 207 1168 3118
Fragments arranged by size:
3118 1168 577 380 309 207 156 147
85 78 71 46 1
EcoRI G'AATT_C
Cuts at: 3345 3345
Size: 6343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 567 4360 567
Size: 3793 2550
FspI TGC'GCA
Cuts at: 4122 4122
Size: 6343
HaeII r_GCGC'y
Cuts at: 1876 2006 3775 4023 5047 6026 1876
Size: 130 1769 248 1024 979 2193
Fragments arranged by size:
2193 1769 1024 979 248 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1927 1959 4183 4213 4215 4245 1927
Size: 32 2224 30 2 30 4025
Fragments arranged by size:
4025 2224 32 30 30 2
HincII GTy'rAC
Cuts at: 633 2475 3380 633
Size: 1842 905 3596
Fragments arranged by size:
3596 1842 905
HindIII A'AGCT_T
Cuts at: 688 688
Size: 6343
KasI G'GCGC_C
Cuts at: 3771 4019 3771
Size: 248 6095
KpnI G_GTAC'C
Cuts at: 450 3361 450
Size: 2911 3432
MluI A'CGCG_T
Cuts at: 5776 5776
Size: 6343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 732 953 1613 1733 5014 5198 732
Size: 221 660 120 3281 184 1877
Fragments arranged by size:
3281 1877 660 221 184 120
MscI TGG'CCA
Cuts at: 3193 4102 3193
Size: 909 5434
MslI CAynn'nnrTG
Cuts at: 422 491 4330 4457 422
Size: 69 3839 127 2308
Fragments arranged by size:
3839 2308 127 69
MspA1I CmG'CkG
Cuts at: 351 374 444 510 580 619 1105 1260 1275
Size: 23 70 66 70 39 486 155 15
Cuts at: 1275 1320 1380 1395 1548 1599 1665 1821 2020
Size: 45 60 15 153 51 66 156 199
Cuts at: 2020 2340 2412 2781 2794 2874 3452 3766 4126
Size: 320 72 369 13 80 578 314 360
Cuts at: 4126 5141 5386 351
Size: 1015 245 1308
Fragments arranged by size:
1308 1015 578 486 369 360 320 314
245 199 156 155 153 80 72 70
70 66 66 60 51 45 39 23
15 15 13
NaeI GCC'GGC
Cuts at: 149 1927 4522 149
Size: 1778 2595 1970
Fragments arranged by size:
2595 1970 1778
NarI GG'CG_CC
Cuts at: 3772 4020 3772
Size: 248 6095
NcoI C'CATG_G
Cuts at: 3020 3020
Size: 6343
NgoMIV G'CCGG_C
Cuts at: 147 1925 4520 147
Size: 1778 2595 1970
Fragments arranged by size:
2595 1970 1778
NruI TCG'CGA
Cuts at: 4704 4704
Size: 6343
NspI r_CATG'y
Cuts at: 936 2370 3123 3394 3894 4803 936
Size: 1434 753 271 500 909 2476
Fragments arranged by size:
2476 1434 909 753 500 271
PciI A'CATG_T
Cuts at: 4799 4799
Size: 6343
PflMI CCAn_nnn'nTGG
Cuts at: 183 1371 2877 183
Size: 1188 1506 3649
Fragments arranged by size:
3649 1506 1188
PfoI T'CCnGG_A
Cuts at: 461 785 3086 461
Size: 324 2301 3718
Fragments arranged by size:
3718 2301 324
PmeI GTTT'AAAC
Cuts at: 3340 3340
Size: 6343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5482 5514 5482
Size: 32 6311
PpuMI rG'GwC_Cy
Cuts at: 402 979 1182 402
Size: 577 203 5563
Fragments arranged by size:
5563 577 203
PshAI GACnn'nnGTC
Cuts at: 628 628
Size: 6343
PspOMI G'GGCC_C
Cuts at: 1096 2105 2312 1096
Size: 1009 207 5127
Fragments arranged by size:
5127 1009 207
PstI C_TGCA'G
Cuts at: 796 2033 2302 2345 2399 2660 2933 3388 4073
Size: 1237 269 43 54 261 273 455 685
Cuts at: 4073 796
Size: 3066
Fragments arranged by size:
3066 1237 685 455 273 269 261 54
43
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6343
PvuII CAG'CTG
Cuts at: 374 510 619 1275 1380 1395 1548 1599 1665
Size: 136 109 656 105 15 153 51 66
Cuts at: 1665 2340 2412 2781 2794 2874 3766 4126 374
Size: 675 72 369 13 80 892 360 2591
Fragments arranged by size:
2591 892 675 656 369 360 153 136
109 105 80 72 66 51 15 13
RsrII CG'GwC_CG
Cuts at: 4536 4536
Size: 6343
SacI G_AGCT'C
Cuts at: 1496 1765 2330 2834 3355 1496
Size: 269 565 504 521 4484
Fragments arranged by size:
4484 565 521 504 269
SacII CC_GC'GG
Cuts at: 581 1106 2021 581
Size: 525 915 4903
Fragments arranged by size:
4903 915 525
SalI G'TCGA_C
Cuts at: 2473 3378 2473
Size: 905 5438
SanDI GG'GwC_CC
Cuts at: 979 979
Size: 6343
SapI GCTCTTCn'nnn_
Cuts at: 1273 1648 4364 4574 1273
Size: 375 2716 210 3042
Fragments arranged by size:
3042 2716 375 210
SbfI CC_TGCA'GG
Cuts at: 2033 2302 2399 2933 3388 2033
Size: 269 97 534 455 4988
Fragments arranged by size:
4988 534 455 269 97
ScaI AGT'ACT
Cuts at: 5860 5860
Size: 6343
SexAI A'CCwGG_T
Cuts at: 2093 2093
Size: 6343
SfcI C'TryA_G
Cuts at: 33 792 2029 2298 2341 2395 2656 2929 3384
Size: 759 1237 269 43 54 261 273 455
Cuts at: 3384 4069 5064 5255 33
Size: 685 995 191 1121
Fragments arranged by size:
1237 1121 995 759 685 455 273 269
261 191 54 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 2113 2113
Size: 6343
SfoI GGC'GCC
Cuts at: 3773 4021 3773
Size: 248 6095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6343
SmaI CCC'GGG
Cuts at: 2317 3363 2317
Size: 1046 5297
SmlI C'TyrA_G
Cuts at: 559 1675 3496 4411 4905 5167 559
Size: 1116 1821 915 494 262 1735
Fragments arranged by size:
1821 1735 1116 915 494 262
SnaBI TAC'GTA
Cuts at: 3333 3333
Size: 6343
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6343
SphI G_CATG'C
Cuts at: 3123 3394 3894 3123
Size: 271 500 5572
Fragments arranged by size:
5572 500 271
SrfI GCCC'GGGC
Cuts at: 2317 2317
Size: 6343
StuI AGG'CCT
Cuts at: 2116 2738 2116
Size: 622 5721
StyI C'CwwG_G
Cuts at: 1694 2117 2168 2753 2888 3020 3475 3829 1694
Size: 423 51 585 135 132 455 354 4208
Fragments arranged by size:
4208 585 455 423 354 135 132 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 640 2746 3924 4057 4516 4727 262
Size: 378 2106 1178 133 459 211 1878
Fragments arranged by size:
2106 1878 1178 459 378 211 133
TatI w'GTAC_w
Cuts at: 938 5858 938
Size: 4920 1423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 497 529 1431 1463 3173 3205 5860 5892 497
Size: 32 902 32 1710 32 2655 32 948
Fragments arranged by size:
2655 1710 948 902 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4138 4138
Size: 6343
XbaI T'CTAG_A
Cuts at: 3372 3372
Size: 6343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2256 2781 3836 2256
Size: 525 1055 4763
Fragments arranged by size:
4763 1055 525
XmaI C'CCGG_G
Cuts at: 2315 3361 2315
Size: 1046 5297
XmnI GAAnn'nnTTC
Cuts at: 913 913
Size: 6343
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsrGI BstEII BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NsiI PacI PasI PmlI PsiI PspXI
PsrI SgrAI SspI SwaI XhoI ZraI