Restriction map for
pF1KSDA0649
(Circular) (Six-base) MAPSORT of: pF1KSDA0649.seq Check: 3807 from: 1 to: 6766
pF1KSDA0649 6766 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 3752 3802 3752
Size: 50 6716
Acc65I G'GTAC_C
Cuts at: 3780 3780
Size: 6766
AclI AA'CG_TT
Cuts at: 6606 6606
Size: 6766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1091 1495 2186 2395 3417 3465 3687 4589
Size: 523 404 691 209 1022 48 222 902
Cuts at: 4589 5021 5770 6038 568
Size: 432 749 268 1296
Fragments arranged by size:
1296 1022 902 749 691 523 432 404
268 222 209 48
AfeI AGC'GCT
Cuts at: 1152 1152
Size: 6766
AflIII A'CryG_T
Cuts at: 4116 5222 6199 4116
Size: 1106 977 4683
Fragments arranged by size:
4683 1106 977
AgeI A'CCGG_T
Cuts at: 5154 5154
Size: 6766
AhdI GACnn_n'nnGTC
Cuts at: 3309 3649 3309
Size: 340 6426
AleI CACnn'nnGTG
Cuts at: 662 662
Size: 6766
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 372 1113 2492 372
Size: 741 1379 4646
Fragments arranged by size:
4646 1379 741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1663 1695 1663
Size: 32 6734
AlwNI CAG_nnn'CTG
Cuts at: 825 963 4069 5638 825
Size: 138 3106 1569 1953
Fragments arranged by size:
3106 1953 1569 138
ApaI G_GGCC'C
Cuts at: 591 2092 2365 2575 2713 2737 3322 3466 591
Size: 1501 273 210 138 24 585 144 3891
Fragments arranged by size:
3891 1501 585 273 210 144 138 24
ApaLI G'TGCA_C
Cuts at: 391 2790 5536 391
Size: 2399 2746 1621
Fragments arranged by size:
2746 2399 1621
ApoI r'AATT_y
Cuts at: 897 3768 6119 6375 897
Size: 2871 2351 256 1288
Fragments arranged by size:
2871 2351 1288 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6766
AvaI C'yCGr_G
Cuts at: 260 305 574 1372 2921 3135 3156 3326 3606
Size: 45 269 798 1549 214 21 170 280
Cuts at: 3606 3784 260
Size: 178 3242
Fragments arranged by size:
3242 1549 798 280 269 214 178 170
45 21
BamHI G'GATC_C
Cuts at: 733 2928 3789 733
Size: 2195 861 3710
Fragments arranged by size:
3710 2195 861
BanI G'GyrC_C
Cuts at: 310 2077 2141 2476 2934 3085 3100 3780 4194
Size: 1767 64 335 458 151 15 680 414
Cuts at: 4194 4442 4477 310
Size: 248 35 2599
Fragments arranged by size:
2599 1767 680 458 414 335 248 151
64 35 15
BanII G_rGCy'C
Cuts at: 591 754 1507 2015 2092 2164 2267 2339 2365
Size: 163 753 508 77 72 103 72 26
Cuts at: 2365 2575 2713 2737 2906 2978 3022 3136 3183
Size: 210 138 24 169 72 44 114 47
Cuts at: 3183 3322 3466 3607 3613 3778 4808 591
Size: 139 144 141 6 165 1030 2549
Fragments arranged by size:
2549 1030 753 508 210 169 165 163
144 141 139 138 114 103 77 72
72 72 47 44 26 24 6
BbeI G_GCGC'C
Cuts at: 2145 3104 4198 4446 2145
Size: 959 1094 248 4465
Fragments arranged by size:
4465 1094 959 248
BbsI GAAGACnn'nnnn_
Cuts at: 2174 2174
Size: 6766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 283 317 283
Size: 34 6732
BciVI GTATCCnnnnn_n'
Cuts at: 4662 5425 6766 4662
Size: 763 1341 4662
Fragments arranged by size:
4662 1341 763
BclI T'GATC_A
Cuts at: 4284 4284
Size: 6766
BglI GCCn_nnn'nGGC
Cuts at: 373 586 968 1759 1850 2059 373
Size: 213 382 791 91 209 5080
Fragments arranged by size:
5080 791 382 213 209 91
BglII A'GATC_T
Cuts at: 4279 6318 4279
Size: 2039 4727
BlpI GC'TnA_GC
Cuts at: 1562 2009 3032 3876 1562
Size: 447 1023 844 4452
Fragments arranged by size:
4452 1023 844 447
Bme1580I G_kGCm'C
Cuts at: 313 395 591 669 2080 2092 2365 2575 2713
Size: 82 196 78 1411 12 273 210 138
Cuts at: 2713 2737 2794 2872 3088 3322 3466 4389 4482
Size: 24 57 78 216 234 144 923 93
Cuts at: 4482 5540 313
Size: 1058 1539
Fragments arranged by size:
1539 1411 1058 923 273 234 216 210
196 144 138 93 82 78 78 57
24 12
BmrI ACTGGGnnnn_n'
Cuts at: 2429 3976 4057 4392 6538 2429
Size: 1547 81 335 2146 2657
Fragments arranged by size:
2657 2146 1547 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5131 5131
Size: 6766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 145 1194 1481 2662 3197 3225 131
Size: 14 1049 287 1181 535 28 3672
Fragments arranged by size:
3672 1181 1049 535 287 28 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2230 3940 4819 5313 5611 2230
Size: 1710 879 494 298 3385
Fragments arranged by size:
3385 1710 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 430 841 1369 2024 2511 6470 430
Size: 411 528 655 487 3959 726
Fragments arranged by size:
3959 726 655 528 487 411
BsaAI yAC'GTr
Cuts at: 3756 4117 3756
Size: 361 6405
BsaBI GATnn'nnATC
Cuts at: 6 274 4278 6317 6
Size: 268 4004 2039 455
Fragments arranged by size:
4004 2039 455 268
BsaHI Gr'CG_yC
Cuts at: 365 971 1192 1201 2142 3101 3287 3677 3692
Size: 606 221 9 941 959 186 390 15
Cuts at: 3692 4195 4443 5147 6643 365
Size: 503 248 704 1496 488
Fragments arranged by size:
1496 959 941 704 606 503 488 390
248 221 186 15 9
BsaWI w'CCGG_w
Cuts at: 1739 2339 2931 3953 4474 5154 5428 5575 5986
Size: 600 592 1022 521 680 274 147 411
Cuts at: 5986 1739
Size: 2519
Fragments arranged by size:
2519 1022 680 600 592 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 736 766 1473 1503 1687 1717 232
Size: 30 474 30 707 30 184 30 5281
Fragments arranged by size:
5281 707 474 184 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 541 1246 1327 2529 3615 469
Size: 72 705 81 1202 1086 3620
Fragments arranged by size:
3620 1202 1086 705 81 72
BseYI C'CCAG_C
Cuts at: 590 605 966 1393 1893 2328 2574 2581 2736
Size: 15 361 427 500 435 246 7 155
Cuts at: 2736 3067 3151 3235 3337 3377 3502 4189 4947
Size: 331 84 84 102 40 125 687 758
Cuts at: 4947 5526 6022 590
Size: 579 496 1334
Fragments arranged by size:
1334 758 687 579 500 496 435 427
361 331 246 155 125 102 84 84
40 15 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 286 620 1595 2277 2298 2778 286
Size: 334 975 682 21 480 4274
Fragments arranged by size:
4274 975 682 480 334 21
BsiEI CG_ry'CG
Cuts at: 104 3192 4306 4352 5562 104
Size: 3088 1114 46 1210 1308
Fragments arranged by size:
3088 1308 1210 1114 46
BsiHKAI G_wGCw'C
Cuts at: 395 2164 2794 2865 2950 3607 3778 4556 4746
Size: 1769 630 71 85 657 171 778 190
Cuts at: 4746 5540 395
Size: 794 1621
Fragments arranged by size:
1769 1621 794 778 657 630 190 171
85 71
Bsp1286I G_dGCh'C
Cuts at: 313 395 591 669 754 1507 2015 2080 2092
Size: 82 196 78 85 753 508 65 12
Cuts at: 2092 2164 2267 2339 2365 2575 2713 2737 2794
Size: 72 103 72 26 210 138 24 57
Cuts at: 2794 2865 2872 2906 2950 2978 3022 3088 3136
Size: 71 7 34 44 28 44 66 48
Cuts at: 3136 3183 3322 3466 3607 3613 3778 4389 4482
Size: 47 139 144 141 6 165 611 93
Cuts at: 4482 4556 4746 4808 5540 313
Size: 74 190 62 732 1539
Fragments arranged by size:
1539 753 732 611 508 210 196 190
165 144 141 139 138 103 93 85
82 78 74 72 72 71 66 65
62 57 48 47 44 44 34 28
26 24 12 7 6
BspEI T'CCGG_A
Cuts at: 1739 2339 5986 1739
Size: 600 3647 2519
Fragments arranged by size:
3647 2519 600
BspHI T'CATG_A
Cuts at: 134 1175 5942 134
Size: 1041 4767 958
Fragments arranged by size:
4767 1041 958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1295 2042 3814 4028 4330 4711 1295
Size: 747 1772 214 302 381 3350
Fragments arranged by size:
3350 1772 747 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1685 2719 3163 5056 5110 6596 6761 50
Size: 1635 1034 444 1893 54 1486 165 55
Fragments arranged by size:
1893 1635 1486 1034 444 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4676 4676
Size: 6766
BsrFI r'CCGG_y
Cuts at: 372 580 651 1086 1611 1849 2769 4762 4943
Size: 208 71 435 525 238 920 1993 181
Cuts at: 4943 5154 372
Size: 211 1984
Fragments arranged by size:
1993 1984 920 525 435 238 211 208
181 71
BssHII G'CGCG_C
Cuts at: 324 324
Size: 6766
BssSI C'ACGA_G
Cuts at: 1539 3023 5035 5395 1539
Size: 1484 2012 360 2910
Fragments arranged by size:
2910 2012 1484 360
BstAPI GCAn_nnn'nTGC
Cuts at: 963 4141 963
Size: 3178 3588
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6766
BstXI CCAn_nnnn'nTGG
Cuts at: 1425 1425
Size: 6766
BstYI r'GATC_y
Cuts at: 1 733 2928 3789 4256 4279 4614 4860 5874
Size: 732 2195 861 467 23 335 246 1014
Cuts at: 5874 5960 5972 6318 1
Size: 86 12 346 449
Fragments arranged by size:
2195 1014 861 732 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1039 3634 1039
Size: 2595 4171
BtgI C'CryG_G
Cuts at: 446 509 1424 1706 3241 3716 446
Size: 63 915 282 1535 475 3496
Fragments arranged by size:
3496 1535 915 475 282 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 332 794 1730 4714 4895 5058 332
Size: 462 936 2984 181 163 2040
Fragments arranged by size:
2984 2040 936 462 181 163
BtsI GCAGTG_nn'
Cuts at: 669 1356 1635 1888 3278 3693 4007 669
Size: 687 279 253 1390 415 314 3428
Fragments arranged by size:
3428 1390 687 415 314 279 253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 392 427 2703 2738 392
Size: 35 2276 35 4420
Fragments arranged by size:
4420 2276 35 35
DraI TTT'AAA
Cuts at: 3763 3763
Size: 6766
DrdI GACnn_nn'nnGTC
Cuts at: 4470 5330 4470
Size: 860 5906
EaeI y'GGCC_r
Cuts at: 1412 1781 1854 1923 2016 2828 3556 4349 4523
Size: 369 73 69 93 812 728 793 174
Cuts at: 4523 4914 4941 6288 1412
Size: 391 27 1347 1890
Fragments arranged by size:
1890 1347 812 793 728 391 369 174
93 73 69 27
EagI C'GGCC_G
Cuts at: 4349 4349
Size: 6766
EarI CTCTTCn'nnn_
Cuts at: 829 1663 2245 2371 3388 3436 4787 4997 829
Size: 834 582 126 1017 48 1351 210 2598
Fragments arranged by size:
2598 1351 1017 834 582 210 126 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 641 744 823 891 1564 2517 2677 2955 4972
Size: 103 79 68 673 953 160 278 2017
Cuts at: 4972 5284 5430 6576 641
Size: 312 146 1146 831
Fragments arranged by size:
2017 1146 953 831 673 312 278 160
146 103 79 68
EcoICRI GAG'CTC
Cuts at: 2162 3605 3776 2162
Size: 1443 171 5152
Fragments arranged by size:
5152 1443 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 131 145 568 1091 1194 1481 1495 2186 2395
Size: 14 423 523 103 287 14 691 209
Cuts at: 2395 2662 3197 3225 3417 3465 3687 4589 5021
Size: 267 535 28 192 48 222 902 432
Cuts at: 5021 5770 6038 131
Size: 749 268 859
Fragments arranged by size:
902 859 749 691 535 523 432 423
287 268 267 222 209 192 103 48
28 14 14
EcoNI CCTnn'n_nnAGG
Cuts at: 704 1436 2921 704
Size: 732 1485 4549
Fragments arranged by size:
4549 1485 732
EcoO109I rG'GnC_Cy
Cuts at: 587 960 1284 1443 1637 2061 2088 2089 2199
Size: 373 324 159 194 424 27 1 110
Cuts at: 2199 2361 2416 2571 2709 2710 2733 2817 3318
Size: 162 55 155 138 1 23 84 501
Cuts at: 3318 3319 3330 3462 3463 3903 587
Size: 1 11 132 1 440 3450
Fragments arranged by size:
3450 501 440 424 373 324 194 162
159 155 138 132 110 84 55 27
23 11 1 1 1 1
EcoRI G'AATT_C
Cuts at: 3768 3768
Size: 6766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 930 4783 930
Size: 3853 2913
FspI TGC'GCA
Cuts at: 4545 4545
Size: 6766
HaeII r_GCGC'y
Cuts at: 260 1148 1154 1324 2145 3104 4198 4446 5470
Size: 888 6 170 821 959 1094 248 1024
Cuts at: 5470 6449 260
Size: 979 577
Fragments arranged by size:
1094 1024 979 959 888 821 577 248
170 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 736 768 1687 1719 3295 3327 3635
Size: 32 472 32 919 32 1576 32 308
Cuts at: 3635 3667 4606 4636 4638 4668 232
Size: 32 939 30 2 30 2330
Fragments arranged by size:
2330 1576 939 919 472 308 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3654 3803 3654
Size: 149 6617
KasI G'GCGC_C
Cuts at: 2141 3100 4194 4442 2141
Size: 959 1094 248 4465
Fragments arranged by size:
4465 1094 959 248
KpnI G_GTAC'C
Cuts at: 3784 3784
Size: 6766
MluI A'CGCG_T
Cuts at: 6199 6199
Size: 6766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1326 5437 5621 1229
Size: 97 4111 184 2374
Fragments arranged by size:
4111 2374 184 97
MscI TGG'CCA
Cuts at: 1414 1783 1925 3558 4525 1414
Size: 369 142 1633 967 3655
Fragments arranged by size:
3655 1633 967 369 142
MslI CAynn'nnrTG
Cuts at: 662 2104 4753 4880 662
Size: 1442 2649 127 2548
Fragments arranged by size:
2649 2548 1442 127
MspA1I CmG'CkG
Cuts at: 1245 1254 2328 2875 2954 3063 3071 3082 3366
Size: 9 1074 547 79 109 8 11 284
Cuts at: 3366 3875 4189 4549 5564 5809 1245
Size: 509 314 360 1015 245 2202
Fragments arranged by size:
2202 1074 1015 547 509 360 314 284
245 109 79 11 9 8
NaeI GCC'GGC
Cuts at: 374 582 1088 1613 2771 4945 374
Size: 208 506 525 1158 2174 2195
Fragments arranged by size:
2195 2174 1158 525 506 208
NarI GG'CG_CC
Cuts at: 2142 3101 4195 4443 2142
Size: 959 1094 248 4465
Fragments arranged by size:
4465 1094 959 248
NcoI C'CATG_G
Cuts at: 446 1424 446
Size: 978 5788
NgoMIV G'CCGG_C
Cuts at: 372 580 1086 1611 2769 4943 372
Size: 208 506 525 1158 2174 2195
Fragments arranged by size:
2195 2174 1158 525 506 208
NruI TCG'CGA
Cuts at: 5127 5127
Size: 6766
NspI r_CATG'y
Cuts at: 2109 3817 4317 5226 2109
Size: 1708 500 909 3649
Fragments arranged by size:
3649 1708 909 500
PasI CC'CwG_GG
Cuts at: 1812 2064 2267 2558 2568 1812
Size: 252 203 291 10 6010
Fragments arranged by size:
6010 291 252 203 10
PciI A'CATG_T
Cuts at: 5222 5222
Size: 6766
PflMI CCAn_nnn'nTGG
Cuts at: 2782 3242 2782
Size: 460 6306
PfoI T'CCnGG_A
Cuts at: 536 1513 1586 536
Size: 977 73 5716
Fragments arranged by size:
5716 977 73
PmeI GTTT'AAAC
Cuts at: 3763 3763
Size: 6766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5905 5937 5905
Size: 32 6734
PpuMI rG'GwC_Cy
Cuts at: 1284 1637 2199 2416 2817 1284
Size: 353 562 217 401 5233
Fragments arranged by size:
5233 562 401 353 217
PshAI GACnn'nnGTC
Cuts at: 1433 1433
Size: 6766
PspOMI G'GGCC_C
Cuts at: 587 2088 2361 2571 2709 2733 3318 3462 587
Size: 1501 273 210 138 24 585 144 3891
Fragments arranged by size:
3891 1501 585 273 210 144 138 24
PstI C_TGCA'G
Cuts at: 290 1259 3461 3811 4496 290
Size: 969 2202 350 685 2560
Fragments arranged by size:
2560 2202 969 685 350
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6766
PvuII CAG'CTG
Cuts at: 1245 1254 2328 2875 3063 3071 4189 4549 1245
Size: 9 1074 547 188 8 1118 360 3462
Fragments arranged by size:
3462 1118 1074 547 360 188 9 8
RsrII CG'GwC_CG
Cuts at: 4959 4959
Size: 6766
SacI G_AGCT'C
Cuts at: 2164 3607 3778 2164
Size: 1443 171 5152
Fragments arranged by size:
5152 1443 171
SalI G'TCGA_C
Cuts at: 3801 3801
Size: 6766
SanDI GG'GwC_CC
Cuts at: 1637 2817 1637
Size: 1180 5586
SapI GCTCTTCn'nnn_
Cuts at: 2371 3388 4787 4997 2371
Size: 1017 1399 210 4140
Fragments arranged by size:
4140 1399 1017 210
SbfI CC_TGCA'GG
Cuts at: 3811 3811
Size: 6766
ScaI AGT'ACT
Cuts at: 6283 6283
Size: 6766
SfcI C'TryA_G
Cuts at: 33 198 286 464 1255 1619 3457 3807 4492
Size: 165 88 178 791 364 1838 350 685
Cuts at: 4492 5487 5678 33
Size: 995 191 1121
Fragments arranged by size:
1838 1121 995 791 685 364 350 191
178 165 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 586 586
Size: 6766
SfoI GGC'GCC
Cuts at: 2143 3102 4196 4444 2143
Size: 959 1094 248 4465
Fragments arranged by size:
4465 1094 959 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6766
SmaI CCC'GGG
Cuts at: 262 576 3158 3328 3786 262
Size: 314 2582 170 458 3242
Fragments arranged by size:
3242 2582 458 314 170
SmlI C'TyrA_G
Cuts at: 2245 3919 4834 5328 5590 2245
Size: 1674 915 494 262 3421
Fragments arranged by size:
3421 1674 915 494 262
SnaBI TAC'GTA
Cuts at: 3756 3756
Size: 6766
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6766
SphI G_CATG'C
Cuts at: 3817 4317 3817
Size: 500 6266
StuI AGG'CCT
Cuts at: 178 178
Size: 6766
StyI C'CwwG_G
Cuts at: 446 1046 1424 2672 2969 3680 3898 4252 446
Size: 600 378 1248 297 711 218 354 2960
Fragments arranged by size:
2960 1248 711 600 378 354 297 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1407 3234 3391 4347 4480 4939 5150 1407
Size: 1827 157 956 133 459 211 3023
Fragments arranged by size:
3023 1827 956 459 211 157 133
TatI w'GTAC_w
Cuts at: 6281 6281
Size: 6766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6283 6315 6283
Size: 32 6734
Tth111I GACn'n_nGTC
Cuts at: 4561 4561
Size: 6766
XbaI T'CTAG_A
Cuts at: 3795 3795
Size: 6766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 859 1422 2061 4259 859
Size: 563 639 2198 3366
Fragments arranged by size:
3366 2198 639 563
XmaI C'CCGG_G
Cuts at: 260 574 3156 3326 3784 260
Size: 314 2582 170 458 3242
Fragments arranged by size:
3242 2582 458 314 170
XmnI GAAnn'nnTTC
Cuts at: 821 2551 2837 821
Size: 1730 286 4750
Fragments arranged by size:
4750 1730 286
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AscI AvrII BaeI BbvCI BfrBI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NsiI PacI PmlI PsiI
PspXI PsrI SacII SexAI SgrAI SrfI SspI SwaI
XhoI ZraI