Restriction map for
pF1KSDA0637
(Circular) (Six-base) MAPSORT of: pF1KSDA0637.seq Check: 4174 from: 1 to: 6652
pF1KSDA0637 6652 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2436 2436
Size: 6652
AatII G_ACGT'C
Cuts at: 2789 2789
Size: 6652
AccI GT'mk_AC
Cuts at: 2153 3688 2153
Size: 1535 5117
Acc65I G'GTAC_C
Cuts at: 1912 3666 1912
Size: 1754 4898
AclI AA'CG_TT
Cuts at: 2159 6492 2159
Size: 4333 2319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 842 1516 2512 2592 3100 3389 3463 3500 4475
Size: 674 996 80 508 289 74 37 975
Cuts at: 4475 4907 5656 5924 842
Size: 432 749 268 1570
Fragments arranged by size:
1570 996 975 749 674 508 432 289
268 80 74 37
AflII C'TTAA_G
Cuts at: 2284 2284
Size: 6652
AflIII A'CryG_T
Cuts at: 839 1115 2438 4002 5108 6085 839
Size: 276 1323 1564 1106 977 1406
Fragments arranged by size:
1564 1406 1323 1106 977 276
AgeI A'CCGG_T
Cuts at: 2708 5040 2708
Size: 2332 4320
AhdI GACnn_n'nnGTC
Cuts at: 1317 2790 1317
Size: 1473 5179
AleI CACnn'nnGTG
Cuts at: 2008 3269 3416 2008
Size: 1261 147 5244
Fragments arranged by size:
5244 1261 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 448 480 448
Size: 32 6620
AlwNI CAG_nnn'CTG
Cuts at: 2670 3955 5524 2670
Size: 1285 1569 3798
Fragments arranged by size:
3798 1569 1285
ApaLI G'TGCA_C
Cuts at: 2704 5422 2704
Size: 2718 3934
ApoI r'AATT_y
Cuts at: 218 3234 3654 6005 6261 218
Size: 3016 420 2351 256 609
Fragments arranged by size:
3016 2351 609 420 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6652
AvaI C'yCGr_G
Cuts at: 336 509 2006 2592 2821 3670 336
Size: 173 1497 586 229 849 3318
Fragments arranged by size:
3318 1497 849 586 229 173
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 567 600 1896 1929 567
Size: 33 1296 33 5290
Fragments arranged by size:
5290 1296 33 33
BamHI G'GATC_C
Cuts at: 3150 3675 3150
Size: 525 6127
BanI G'GyrC_C
Cuts at: 1456 1603 1634 1912 2010 3666 4080 4328 4363
Size: 147 31 278 98 1656 414 248 35
Cuts at: 4363 1456
Size: 3745
Fragments arranged by size:
3745 1656 414 278 248 147 98 35
31
BanII G_rGCy'C
Cuts at: 466 1232 3664 4694 466
Size: 766 2432 1030 2424
Fragments arranged by size:
2432 2424 1030 766
BbeI G_GCGC'C
Cuts at: 1638 4084 4332 1638
Size: 2446 248 3958
Fragments arranged by size:
3958 2446 248
BbsI GAAGACnn'nnnn_
Cuts at: 1358 1915 2575 3126 1358
Size: 557 660 551 4884
Fragments arranged by size:
4884 660 557 551
BbvCI CC'TCA_GC
Cuts at: 721 2765 2986 721
Size: 2044 221 4387
Fragments arranged by size:
4387 2044 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 904 938 904
Size: 34 6618
BciVI GTATCCnnnnn_n'
Cuts at: 4548 5311 6652 4548
Size: 763 1341 4548
Fragments arranged by size:
4548 1341 763
BclI T'GATC_A
Cuts at: 2888 4170 2888
Size: 1282 5370
BfrBI ATG'CAT
Cuts at: 533 533
Size: 6652
BglI GCCn_nnn'nGGC
Cuts at: 424 1664 1708 2397 424
Size: 1240 44 689 4679
Fragments arranged by size:
4679 1240 689 44
BglII A'GATC_T
Cuts at: 2690 4165 6204 2690
Size: 1475 2039 3138
Fragments arranged by size:
3138 2039 1475
BlpI GC'TnA_GC
Cuts at: 1231 2674 3762 1231
Size: 1443 1088 4121
Fragments arranged by size:
4121 1443 1088
Bme1580I G_kGCm'C
Cuts at: 1096 1128 1162 1606 2023 2708 4275 4368 5426
Size: 32 34 444 417 685 1567 93 1058
Cuts at: 5426 1096
Size: 2322
Fragments arranged by size:
2322 1567 1058 685 444 417 93 34
32
BmgBI CAC'GTC
Cuts at: 414 1356 2318 2439 3252 414
Size: 942 962 121 813 3814
Fragments arranged by size:
3814 962 942 813 121
BmrI ACTGGGnnnn_n'
Cuts at: 384 2077 2382 3862 3943 4278 6424 384
Size: 1693 305 1480 81 335 2146 612
Fragments arranged by size:
2146 1693 1480 612 335 305 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 322 1251 5017 322
Size: 929 3766 1957
Fragments arranged by size:
3766 1957 929
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 464 1355 2119 2211 2509 3412 450
Size: 14 891 764 92 298 903 3690
Fragments arranged by size:
3690 903 891 764 298 92 14
Bpu10I CC'TnA_GC
Cuts at: 721 2765 2986 721
Size: 2044 221 4387
Fragments arranged by size:
4387 2044 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1528 3826 4705 5199 5497 600
Size: 928 2298 879 494 298 1755
Fragments arranged by size:
2298 1755 928 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1977 6356 1977
Size: 4379 2273
BsaAI yAC'GTr
Cuts at: 606 3642 4003 606
Size: 3036 361 3255
Fragments arranged by size:
3255 3036 361
BsaBI GATnn'nnATC
Cuts at: 6 3055 4164 6203 6
Size: 3049 1109 2039 455
Fragments arranged by size:
3049 2039 1109 455
BsaHI Gr'CG_yC
Cuts at: 763 1399 1635 2786 3451 4081 4329 5033 6529
Size: 636 236 1151 665 630 248 704 1496
Cuts at: 6529 763
Size: 886
Fragments arranged by size:
1496 1151 886 704 665 636 630 248
236
BsaWI w'CCGG_w
Cuts at: 1251 1998 2708 3839 4360 5040 5314 5461 5872
Size: 747 710 1131 521 680 274 147 411
Cuts at: 5872 1251
Size: 2031
Fragments arranged by size:
2031 1131 747 710 680 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 790 820 2117 2147 2482 2512 3151
Size: 30 312 30 1297 30 335 30 639
Cuts at: 3151 3181 3301 3331 448
Size: 30 120 30 3769
Fragments arranged by size:
3769 1297 639 335 312 120 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 811 822 1275 1278 1983 3058 3196 3199 3322
Size: 11 453 3 705 1075 138 3 123
Cuts at: 3322 3409 3412 811
Size: 87 3 4051
Fragments arranged by size:
4051 1075 705 453 138 123 87 11
3 3 3
BseYI C'CCAG_C
Cuts at: 4075 4833 5412 5908 4075
Size: 758 579 496 4819
Fragments arranged by size:
4819 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2620 2683 2620
Size: 63 6589
BsiEI CG_ry'CG
Cuts at: 104 626 786 2401 2713 4192 4238 5448 104
Size: 522 160 1615 312 1479 46 1210 1308
Fragments arranged by size:
1615 1479 1308 1210 522 312 160 46
BsiHKAI G_wGCw'C
Cuts at: 466 632 967 1232 2591 2708 2806 2971 3664
Size: 166 335 265 1359 117 98 165 693
Cuts at: 3664 4442 4632 5426 466
Size: 778 190 794 1692
Fragments arranged by size:
1692 1359 794 778 693 335 265 190
166 165 117 98
BsmI GAATG_Cn'
Cuts at: 1043 2474 1043
Size: 1431 5221
BsmBI CGTCTCn'nnnn_
Cuts at: 893 1253 1972 3040 893
Size: 360 719 1068 4505
Fragments arranged by size:
4505 1068 719 360
Bsp1286I G_dGCh'C
Cuts at: 466 632 967 1096 1128 1162 1232 1606 2023
Size: 166 335 129 32 34 70 374 417
Cuts at: 2023 2591 2708 2806 2971 3664 4275 4368 4442
Size: 568 117 98 165 693 611 93 74
Cuts at: 4442 4632 4694 5426 466
Size: 190 62 732 1692
Fragments arranged by size:
1692 732 693 611 568 417 374 335
190 166 165 129 117 98 93 74
70 62 34 32
BspEI T'CCGG_A
Cuts at: 1998 5872 1998
Size: 3874 2778
BspHI T'CATG_A
Cuts at: 596 1484 3230 3572 5828 596
Size: 888 1746 342 2256 1420
Fragments arranged by size:
2256 1746 1420 888 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 930 2436 2678 3700 3914 4216 4597 930
Size: 1506 242 1022 214 302 381 2985
Fragments arranged by size:
2985 1506 1022 381 302 242 214
BsrBI CCG'CTC
Cuts at: 50 301 2700 2826 3426 4942 4996 6482 6647
Size: 251 2399 126 600 1516 54 1486 165
Cuts at: 6647 50
Size: 55
Fragments arranged by size:
2399 1516 1486 600 251 165 126 55
54
BsrDI GCAATG_nn'
Cuts at: 4562 4562
Size: 6652
BsrFI r'CCGG_y
Cuts at: 1260 1631 2521 2708 4648 4829 5040 1260
Size: 371 890 187 1940 181 211 2872
Fragments arranged by size:
2872 1940 890 371 211 187 181
BssHII G'CGCG_C
Cuts at: 377 612 377
Size: 235 6417
BssSI C'ACGA_G
Cuts at: 1095 4921 5281 1095
Size: 3826 360 2466
Fragments arranged by size:
3826 2466 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1049 1100 1610 2670 4027 1049
Size: 51 510 1060 1357 3674
Fragments arranged by size:
3674 1357 1060 510 51
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6652
BstEII G'GTnAC_C
Cuts at: 715 2511 715
Size: 1796 4856
BstXI CCAn_nnnn'nTGG
Cuts at: 3147 3147
Size: 6652
BstYI r'GATC_y
Cuts at: 1 770 2690 3150 3675 4142 4165 4500 4746
Size: 769 1920 460 525 467 23 335 246
Cuts at: 4746 5760 5846 5858 6204 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1920 1014 769 525 467 460 449 346
335 246 86 23 12
BtgI C'CryG_G
Cuts at: 125 419 1532 2014 3002 3089 3124 3374 125
Size: 294 1113 482 988 87 35 250 3403
Fragments arranged by size:
3403 1113 988 482 294 250 87 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4600 4781 4944 4600
Size: 181 163 6308
Fragments arranged by size:
6308 181 163
BtsI GCAGTG_nn'
Cuts at: 1047 2418 2608 3893 1047
Size: 1371 190 1285 3806
Fragments arranged by size:
3806 1371 1285 190
DraI TTT'AAA
Cuts at: 192 252 3649 192
Size: 60 3397 3195
Fragments arranged by size:
3397 3195 60
DrdI GACnn_nn'nnGTC
Cuts at: 4356 5216 4356
Size: 860 5792
EaeI y'GGCC_r
Cuts at: 416 1872 2398 2735 3329 3457 3476 3558 4235
Size: 1456 526 337 594 128 19 82 677
Cuts at: 4235 4409 4800 4827 6174 416
Size: 174 391 27 1347 894
Fragments arranged by size:
1456 1347 894 677 594 526 391 337
174 128 82 27 19
EagI C'GGCC_G
Cuts at: 2398 4235 2398
Size: 1837 4815
EarI CTCTTCn'nnn_
Cuts at: 194 937 1734 3043 4673 4883 194
Size: 743 797 1309 1630 210 1963
Fragments arranged by size:
1963 1630 1309 797 743 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3205 4858 5170 5316 6462 3205
Size: 1653 312 146 1146 3395
Fragments arranged by size:
3395 1653 1146 312 146
EcoICRI GAG'CTC
Cuts at: 464 1230 3662 464
Size: 766 2432 3454
Fragments arranged by size:
3454 2432 766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 464 842 1355 1516 2119 2211 2509 2512
Size: 14 378 513 161 603 92 298 3
Cuts at: 2512 2592 3100 3389 3412 3463 3500 4475 4907
Size: 80 508 289 23 51 37 975 432
Cuts at: 4907 5656 5924 450
Size: 749 268 1178
Fragments arranged by size:
1178 975 749 603 513 508 432 378
298 289 268 161 92 80 51 37
23 14 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2779 2989 3264 3445 2779
Size: 210 275 181 5986
Fragments arranged by size:
5986 275 210 181
EcoO109I rG'GnC_Cy
Cuts at: 1086 3789 1086
Size: 2703 3949
EcoRI G'AATT_C
Cuts at: 218 3234 3654 218
Size: 3016 420 3216
Fragments arranged by size:
3216 3016 420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1745 3631 4669 1745
Size: 1886 1038 3728
Fragments arranged by size:
3728 1886 1038
FspI TGC'GCA
Cuts at: 4431 4431
Size: 6652
HaeII r_GCGC'y
Cuts at: 1638 2039 2431 4084 4332 5356 6335 1638
Size: 401 392 1653 248 1024 979 1955
Fragments arranged by size:
1955 1653 1024 979 401 392 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 790 822 1303 1335 1608 1640 2776 2808 2873
Size: 32 481 32 273 32 1136 32 65
Cuts at: 2873 2905 3265 3297 4492 4522 4524 4554 790
Size: 32 360 32 1195 30 2 30 2888
Fragments arranged by size:
2888 1195 1136 481 360 273 65 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3689 3689
Size: 6652
KasI G'GCGC_C
Cuts at: 1634 4080 4328 1634
Size: 2446 248 3958
Fragments arranged by size:
3958 2446 248
KpnI G_GTAC'C
Cuts at: 1916 3670 1916
Size: 1754 4898
MluI A'CGCG_T
Cuts at: 6085 6085
Size: 6652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1481 2414 5323 5507 1481
Size: 933 2909 184 2626
Fragments arranged by size:
2909 2626 933 184
MscI TGG'CCA
Cuts at: 418 3331 3560 4411 418
Size: 2913 229 851 2659
Fragments arranged by size:
2913 2659 851 229
MslI CAynn'nnrTG
Cuts at: 914 2008 2974 3001 3269 3416 4639 4766 914
Size: 1094 966 27 268 147 1223 127 2800
Fragments arranged by size:
2800 1223 1094 966 268 147 127 27
MspA1I CmG'CkG
Cuts at: 287 326 991 1610 1919 1941 2016 2487 2655
Size: 39 665 619 309 22 75 471 168
Cuts at: 2655 2894 3126 3416 3761 4075 4435 5450 5695
Size: 239 232 290 345 314 360 1015 245
Cuts at: 5695 287
Size: 1244
Fragments arranged by size:
1244 1015 665 619 471 360 345 314
309 290 245 239 232 168 75 39
22
NaeI GCC'GGC
Cuts at: 4831 4831
Size: 6652
NarI GG'CG_CC
Cuts at: 1635 4081 4329 1635
Size: 2446 248 3958
Fragments arranged by size:
3958 2446 248
NcoI C'CATG_G
Cuts at: 125 419 1532 3002 3089 3374 125
Size: 294 1113 1470 87 285 3403
Fragments arranged by size:
3403 1470 1113 294 285 87
NgoMIV G'CCGG_C
Cuts at: 4829 4829
Size: 6652
NruI TCG'CGA
Cuts at: 5013 5013
Size: 6652
NsiI A_TGCA'T
Cuts at: 535 535
Size: 6652
NspI r_CATG'y
Cuts at: 537 843 1119 2820 3703 4203 5112 537
Size: 306 276 1701 883 500 909 2077
Fragments arranged by size:
2077 1701 909 883 500 306 276
PciI A'CATG_T
Cuts at: 839 1115 5108 839
Size: 276 3993 2383
Fragments arranged by size:
3993 2383 276
PflMI CCAn_nnn'nTGG
Cuts at: 759 2658 759
Size: 1899 4753
PfoI T'CCnGG_A
Cuts at: 466 976 2227 2780 466
Size: 510 1251 553 4338
Fragments arranged by size:
4338 1251 553 510
PmeI GTTT'AAAC
Cuts at: 3649 3649
Size: 6652
PmlI CAC'GTG
Cuts at: 606 606
Size: 6652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 448 480 3212 3244 5791 5823 448
Size: 32 2732 32 2547 32 1277
Fragments arranged by size:
2732 2547 1277 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1086 1086
Size: 6652
PshAI GACnn'nnGTC
Cuts at: 1497 1497
Size: 6652
PspXI vC'TCGA_Gb
Cuts at: 2592 2821 2592
Size: 229 6423
PstI C_TGCA'G
Cuts at: 357 927 1719 2747 3697 4382 357
Size: 570 792 1028 950 685 2627
Fragments arranged by size:
2627 1028 950 792 685 570
PvuI CG_AT'CG
Cuts at: 104 786 104
Size: 682 5970
PvuII CAG'CTG
Cuts at: 326 1610 1919 2487 2894 3416 4075 4435 326
Size: 1284 309 568 407 522 659 360 2543
Fragments arranged by size:
2543 1284 659 568 522 407 360 309
RsrII CG'GwC_CG
Cuts at: 4845 4845
Size: 6652
SacI G_AGCT'C
Cuts at: 466 1232 3664 466
Size: 766 2432 3454
Fragments arranged by size:
3454 2432 766
SacII CC_GC'GG
Cuts at: 2017 3127 2017
Size: 1110 5542
SalI G'TCGA_C
Cuts at: 3687 3687
Size: 6652
SapI GCTCTTCn'nnn_
Cuts at: 937 3043 4673 4883 937
Size: 2106 1630 210 2706
Fragments arranged by size:
2706 2106 1630 210
SbfI CC_TGCA'GG
Cuts at: 3697 3697
Size: 6652
ScaI AGT'ACT
Cuts at: 346 2200 6169 346
Size: 1854 3969 829
Fragments arranged by size:
3969 1854 829
SfcI C'TryA_G
Cuts at: 33 353 809 923 1715 2460 2743 3693 4378
Size: 320 456 114 792 745 283 950 685
Cuts at: 4378 5373 5564 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 950 792 745 685 456 320
283 191 114
SfiI GGCCn_nnn'nGGCC
Cuts at: 424 424
Size: 6652
SfoI GGC'GCC
Cuts at: 1636 4082 4330 1636
Size: 2446 248 3958
Fragments arranged by size:
3958 2446 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6652
SmaI CCC'GGG
Cuts at: 338 2008 3672 338
Size: 1670 1664 3318
Fragments arranged by size:
3318 1670 1664
SmlI C'TyrA_G
Cuts at: 579 1543 2284 2592 2821 3805 4720 5214 5476
Size: 964 741 308 229 984 915 494 262
Cuts at: 5476 579
Size: 1755
Fragments arranged by size:
1755 984 964 915 741 494 308 262
229
SnaBI TAC'GTA
Cuts at: 3642 3642
Size: 6652
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6652
SphI G_CATG'C
Cuts at: 3703 4203 3703
Size: 500 6152
StuI AGG'CCT
Cuts at: 1393 3166 1393
Size: 1773 4879
StyI C'CwwG_G
Cuts at: 125 419 1532 2717 3002 3089 3332 3374 3555
Size: 294 1113 1185 285 87 243 42 181
Cuts at: 3555 3784 4138 125
Size: 229 354 2639
Fragments arranged by size:
2639 1185 1113 354 294 285 243 229
181 87 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 753 1663 2096 2499 3552 4233 4366 4825 5036
Size: 910 433 403 1053 681 133 459 211
Cuts at: 5036 753
Size: 2369
Fragments arranged by size:
2369 1053 910 681 459 433 403 211
133
TatI w'GTAC_w
Cuts at: 344 392 536 1178 2198 3197 6167 344
Size: 48 144 642 1020 999 2970 829
Fragments arranged by size:
2970 1020 999 829 642 144 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1578 1610 2117 2149 2362 2394 2482 2514 3301
Size: 32 507 32 213 32 88 32 787
Cuts at: 3301 3333 6169 6201 1578
Size: 32 2836 32 2029
Fragments arranged by size:
2836 2029 787 507 213 88 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4447 4447
Size: 6652
XbaI T'CTAG_A
Cuts at: 3681 3681
Size: 6652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 3573 4145 2317
Size: 1256 572 4824
Fragments arranged by size:
4824 1256 572
XhoI C'TCGA_G
Cuts at: 2592 2821 2592
Size: 229 6423
XmaI C'CCGG_G
Cuts at: 336 2006 3670 336
Size: 1670 1664 3318
Fragments arranged by size:
3318 1670 1664
XmnI GAAnn'nnTTC
Cuts at: 550 1003 1060 1666 550
Size: 453 57 606 5536
Fragments arranged by size:
5536 606 453 57
ZraI GAC'GTC
Cuts at: 2787 2787
Size: 6652
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AlfI ApaI AscI AvrII BmtI BsiWI BsrGI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI PacI PasI
PsiI PspOMI PsrI SanDI SexAI SgrAI SrfI SspI
SwaI