Restriction map for
pF1KSDA0636
(Circular) (Six-base) MAPSORT of: pF1KSDA0636.seq Check: 2405 from: 1 to: 4750
pF1KSDA0636 4750 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1656 1656
Size: 4750
AccI GT'mk_AC
Cuts at: 1786 1786
Size: 4750
Acc65I G'GTAC_C
Cuts at: 1764 1764
Size: 4750
AclI AA'CG_TT
Cuts at: 4590 4590
Size: 4750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 1491 2573 3005 3754 4022 542
Size: 949 1082 432 749 268 1270
Fragments arranged by size:
1270 1082 949 749 432 268
AflIII A'CryG_T
Cuts at: 2100 3206 4183 2100
Size: 1106 977 2667
Fragments arranged by size:
2667 1106 977
AgeI A'CCGG_T
Cuts at: 3138 3138
Size: 4750
AhdI GACnn_n'nnGTC
Cuts at: 1041 1041
Size: 4750
AleI CACnn'nnGTG
Cuts at: 148 148
Size: 4750
AlwNI CAG_nnn'CTG
Cuts at: 2053 3622 2053
Size: 1569 3181
ApaLI G'TGCA_C
Cuts at: 3520 3520
Size: 4750
ApoI r'AATT_y
Cuts at: 352 616 862 912 996 1204 1752 4103 4359
Size: 264 246 50 84 208 548 2351 256
Cuts at: 4359 352
Size: 743
Fragments arranged by size:
2351 743 548 264 256 246 208 84
50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4750
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4750
AvaI C'yCGr_G
Cuts at: 1768 1768
Size: 4750
BamHI G'GATC_C
Cuts at: 1773 1773
Size: 4750
BanI G'GyrC_C
Cuts at: 1764 2178 2426 2461 1764
Size: 414 248 35 4053
Fragments arranged by size:
4053 414 248 35
BanII G_rGCy'C
Cuts at: 1762 2792 1762
Size: 1030 3720
BbeI G_GCGC'C
Cuts at: 2182 2430 2182
Size: 248 4502
BbsI GAAGACnn'nnnn_
Cuts at: 1273 1273
Size: 4750
BciVI GTATCCnnnnn_n'
Cuts at: 2646 3409 4750 2646
Size: 763 1341 2646
Fragments arranged by size:
2646 1341 763
BclI T'GATC_A
Cuts at: 1415 2268 1415
Size: 853 3897
BfrBI ATG'CAT
Cuts at: 872 872
Size: 4750
BglI GCCn_nnn'nGGC
Cuts at: 1076 1076
Size: 4750
BglII A'GATC_T
Cuts at: 710 2263 4302 710
Size: 1553 2039 1158
Fragments arranged by size:
2039 1553 1158
BlpI GC'TnA_GC
Cuts at: 1860 1860
Size: 4750
Bme1580I G_kGCm'C
Cuts at: 2373 2466 3524 2373
Size: 93 1058 3599
Fragments arranged by size:
3599 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 129 1960 2041 2376 4522 129
Size: 1831 81 335 2146 357
Fragments arranged by size:
2146 1831 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3115 3115
Size: 4750
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 828 1031 1443 828
Size: 203 412 4135
Fragments arranged by size:
4135 412 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 2803 3297 3595 1924
Size: 879 494 298 3079
Fragments arranged by size:
3079 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1049 4454 1049
Size: 3405 1345
BsaAI yAC'GTr
Cuts at: 1335 1740 2101 1335
Size: 405 361 3984
Fragments arranged by size:
3984 405 361
BsaBI GATnn'nnATC
Cuts at: 6 2262 4301 6
Size: 2256 2039 455
Fragments arranged by size:
2256 2039 455
BsaHI Gr'CG_yC
Cuts at: 2179 2427 3131 4627 2179
Size: 248 704 1496 2302
Fragments arranged by size:
2302 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1937 2458 3138 3412 3559 3970 1937
Size: 521 680 274 147 411 2717
Fragments arranged by size:
2717 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1172 1172
Size: 4750
BseYI C'CCAG_C
Cuts at: 1661 1713 2173 2931 3510 4006 1661
Size: 52 460 758 579 496 2405
Fragments arranged by size:
2405 758 579 496 460 52
BsiEI CG_ry'CG
Cuts at: 104 2290 2336 3546 104
Size: 2186 46 1210 1308
Fragments arranged by size:
2186 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1762 2540 2730 3524 1762
Size: 778 190 794 2988
Fragments arranged by size:
2988 794 778 190
BsmI GAATG_Cn'
Cuts at: 874 961 1620 874
Size: 87 659 4004
Fragments arranged by size:
4004 659 87
Bsp1286I G_dGCh'C
Cuts at: 1762 2373 2466 2540 2730 2792 3524 1762
Size: 611 93 74 190 62 732 2988
Fragments arranged by size:
2988 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3970 3970
Size: 4750
BspHI T'CATG_A
Cuts at: 3926 3926
Size: 4750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 498 1182 1656 1798 2012 2314 2695 498
Size: 684 474 142 214 302 381 2553
Fragments arranged by size:
2553 684 474 381 302 214 142
BsrBI CCG'CTC
Cuts at: 50 1550 3040 3094 4580 4745 50
Size: 1500 1490 54 1486 165 55
Fragments arranged by size:
1500 1490 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1248 2660 1248
Size: 1412 3338
BsrFI r'CCGG_y
Cuts at: 2746 2927 3138 2746
Size: 181 211 4358
Fragments arranged by size:
4358 211 181
BssSI C'ACGA_G
Cuts at: 3019 3379 3019
Size: 360 4390
BstAPI GCAn_nnn'nTGC
Cuts at: 2125 2125
Size: 4750
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4750
BstEII G'GTnAC_C
Cuts at: 1033 1033
Size: 4750
BstXI CCAn_nnnn'nTGG
Cuts at: 180 573 759 180
Size: 393 186 4171
Fragments arranged by size:
4171 393 186
BstYI r'GATC_y
Cuts at: 1 585 710 1773 2240 2263 2598 2844 3858
Size: 584 125 1063 467 23 335 246 1014
Cuts at: 3858 3944 3956 4302 1
Size: 86 12 346 449
Fragments arranged by size:
1063 1014 584 467 449 346 335 246
125 86 23 12
BtgI C'CryG_G
Cuts at: 125 1514 125
Size: 1389 3361
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2698 2879 3042 2698
Size: 181 163 4406
Fragments arranged by size:
4406 181 163
BtsI GCAGTG_nn'
Cuts at: 1991 1991
Size: 4750
DraI TTT'AAA
Cuts at: 1747 1747
Size: 4750
DrdI GACnn_nn'nnGTC
Cuts at: 2454 3314 2454
Size: 860 3890
EaeI y'GGCC_r
Cuts at: 1337 1568 2333 2507 2898 2925 4272 1337
Size: 231 765 174 391 27 1347 1815
Fragments arranged by size:
1815 1347 765 391 231 174 27
EagI C'GGCC_G
Cuts at: 2333 2333
Size: 4750
EarI CTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4540
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2956 3268 3414 4560 2956
Size: 312 146 1146 3146
Fragments arranged by size:
3146 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1760 1760
Size: 4750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 828 1031 1443 1491 2573 3005 3754 4022
Size: 286 203 412 48 1082 432 749 268
Cuts at: 4022 542
Size: 1270
Fragments arranged by size:
1270 1082 749 432 412 286 268 203
48
EcoO109I rG'GnC_Cy
Cuts at: 1887 1887
Size: 4750
EcoRI G'AATT_C
Cuts at: 616 912 1752 616
Size: 296 840 3614
Fragments arranged by size:
3614 840 296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 303 1648 2767 303
Size: 1345 1119 2286
Fragments arranged by size:
2286 1345 1119
FspI TGC'GCA
Cuts at: 2529 2529
Size: 4750
HaeII r_GCGC'y
Cuts at: 157 1172 1514 2182 2430 3454 4433 157
Size: 1015 342 668 248 1024 979 474
Fragments arranged by size:
1024 1015 979 668 474 342 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 1027 1059 1167 1199 1362 1394 1568
Size: 32 814 32 108 32 163 32 174
Cuts at: 1568 1600 2590 2620 2622 2652 181
Size: 32 990 30 2 30 2279
Fragments arranged by size:
2279 990 814 174 163 108 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 244 1787 244
Size: 1543 3207
KasI G'GCGC_C
Cuts at: 2178 2426 2178
Size: 248 4502
KpnI G_GTAC'C
Cuts at: 1768 1768
Size: 4750
MluI A'CGCG_T
Cuts at: 4183 4183
Size: 4750
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1467 1473 3421 3605 1467
Size: 6 1948 184 2612
Fragments arranged by size:
2612 1948 184 6
MscI TGG'CCA
Cuts at: 1339 1570 2509 1339
Size: 231 939 3580
Fragments arranged by size:
3580 939 231
MslI CAynn'nnrTG
Cuts at: 148 757 2737 2864 148
Size: 609 1980 127 2034
Fragments arranged by size:
2034 1980 609 127
MspA1I CmG'CkG
Cuts at: 520 993 1859 2173 2533 3548 3793 520
Size: 473 866 314 360 1015 245 1477
Fragments arranged by size:
1477 1015 866 473 360 314 245
NaeI GCC'GGC
Cuts at: 2929 2929
Size: 4750
NarI GG'CG_CC
Cuts at: 2179 2427 2179
Size: 248 4502
NcoI C'CATG_G
Cuts at: 125 1514 125
Size: 1389 3361
NgoMIV G'CCGG_C
Cuts at: 2927 2927
Size: 4750
NruI TCG'CGA
Cuts at: 3111 3111
Size: 4750
NsiI A_TGCA'T
Cuts at: 874 874
Size: 4750
NspI r_CATG'y
Cuts at: 1801 2301 3210 1801
Size: 500 909 3341
Fragments arranged by size:
3341 909 500
PciI A'CATG_T
Cuts at: 3206 3206
Size: 4750
PflMI CCAn_nnn'nTGG
Cuts at: 1159 1668 1159
Size: 509 4241
PmeI GTTT'AAAC
Cuts at: 1747 1747
Size: 4750
PmlI CAC'GTG
Cuts at: 1335 1335
Size: 4750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3889 3921 3889
Size: 32 4718
PstI C_TGCA'G
Cuts at: 495 1356 1795 2480 495
Size: 861 439 685 2765
Fragments arranged by size:
2765 861 685 439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4750
PvuII CAG'CTG
Cuts at: 520 993 2173 2533 520
Size: 473 1180 360 2737
Fragments arranged by size:
2737 1180 473 360
RsrII CG'GwC_CG
Cuts at: 2943 2943
Size: 4750
SacI G_AGCT'C
Cuts at: 1762 1762
Size: 4750
SalI G'TCGA_C
Cuts at: 1785 1785
Size: 4750
SapI GCTCTTCn'nnn_
Cuts at: 2771 2981 2771
Size: 210 4540
SbfI CC_TGCA'GG
Cuts at: 495 1795 495
Size: 1300 3450
ScaI AGT'ACT
Cuts at: 4267 4267
Size: 4750
SfcI C'TryA_G
Cuts at: 33 491 1352 1445 1791 2476 3471 3662 33
Size: 458 861 93 346 685 995 191 1121
Fragments arranged by size:
1121 995 861 685 458 346 191 93
SfoI GGC'GCC
Cuts at: 2180 2428 2180
Size: 248 4502
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4750
SmaI CCC'GGG
Cuts at: 1770 1770
Size: 4750
SmlI C'TyrA_G
Cuts at: 1903 2818 3312 3574 1903
Size: 915 494 262 3079
Fragments arranged by size:
3079 915 494 262
SnaBI TAC'GTA
Cuts at: 1740 1740
Size: 4750
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4750
SphI G_CATG'C
Cuts at: 1801 2301 1801
Size: 500 4250
SspI AAT'ATT
Cuts at: 1384 1384
Size: 4750
StuI AGG'CCT
Cuts at: 702 702
Size: 4750
StyI C'CwwG_G
Cuts at: 125 429 1039 1251 1514 1882 2236 125
Size: 304 610 212 263 368 354 2639
Fragments arranged by size:
2639 610 368 354 304 263 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1262 2331 2464 2923 3134 1262
Size: 1069 133 459 211 2878
Fragments arranged by size:
2878 1069 459 211 133
TatI w'GTAC_w
Cuts at: 4265 4265
Size: 4750
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4267 4299 4267
Size: 32 4718
Tth111I GACn'n_nGTC
Cuts at: 2545 2545
Size: 4750
XbaI T'CTAG_A
Cuts at: 1779 1779
Size: 4750
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1521 2243 1521
Size: 722 4028
XmaI C'CCGG_G
Cuts at: 1768 1768
Size: 4750
XmnI GAAnn'nnTTC
Cuts at: 338 338
Size: 4750
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PciI PflMI
PmeI PmlI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI PacI PasI PfoI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SwaI XhoI ZraI