Restriction map for
pF1KSDA0626
(Circular) (Six-base) MAPSORT of: pF1KSDA0626.seq Check: 8205 from: 1 to: 4366
pF1KSDA0626 4366 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1336 1336
Size: 4366
AccI GT'mk_AC
Cuts at: 578 983 1352 1402 578
Size: 405 369 50 3542
Fragments arranged by size:
3542 405 369 50
Acc65I G'GTAC_C
Cuts at: 1257 1380 1257
Size: 123 4243
AclI AA'CG_TT
Cuts at: 4206 4206
Size: 4366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1256 2189 2621 3370 3638 1024
Size: 232 933 432 749 268 1752
Fragments arranged by size:
1752 933 749 432 268 232
AflIII A'CryG_T
Cuts at: 494 496 1716 2822 3799 494
Size: 2 1220 1106 977 1061
Fragments arranged by size:
1220 1106 1061 977 2
AgeI A'CCGG_T
Cuts at: 2754 2754
Size: 4366
AleI CACnn'nnGTG
Cuts at: 244 499 244
Size: 255 4111
AlwNI CAG_nnn'CTG
Cuts at: 1669 3238 1669
Size: 1569 2797
ApaLI G'TGCA_C
Cuts at: 3136 3136
Size: 4366
ApoI r'AATT_y
Cuts at: 930 1368 3719 3975 930
Size: 438 2351 256 1321
Fragments arranged by size:
2351 1321 438 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4366
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4366
AvaI C'yCGr_G
Cuts at: 1384 1384
Size: 4366
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 452 485 452
Size: 33 4333
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4366
BanI G'GyrC_C
Cuts at: 520 1257 1380 1794 2042 2077 520
Size: 737 123 414 248 35 2809
Fragments arranged by size:
2809 737 414 248 123 35
BanII G_rGCy'C
Cuts at: 259 1378 2408 259
Size: 1119 1030 2217
Fragments arranged by size:
2217 1119 1030
BbeI G_GCGC'C
Cuts at: 1798 2046 1798
Size: 248 4118
BbsI GAAGACnn'nnnn_
Cuts at: 673 673
Size: 4366
BbvCI CC'TCA_GC
Cuts at: 1061 1061
Size: 4366
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 851 1003 1037 817
Size: 34 152 34 4146
Fragments arranged by size:
4146 152 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 201 480 2262 3025 4366 201
Size: 279 1782 763 1341 201
Fragments arranged by size:
1782 1341 763 279 201
BclI T'GATC_A
Cuts at: 1884 1884
Size: 4366
BglII A'GATC_T
Cuts at: 1879 3918 1879
Size: 2039 2327
BlpI GC'TnA_GC
Cuts at: 1476 1476
Size: 4366
Bme1580I G_kGCm'C
Cuts at: 176 266 1989 2082 3140 176
Size: 90 1723 93 1058 1402
Fragments arranged by size:
1723 1402 1058 93 90
BmgBI CAC'GTC
Cuts at: 1217 1217
Size: 4366
BmrI ACTGGGnnnn_n'
Cuts at: 789 1576 1657 1992 4138 789
Size: 787 81 335 2146 1017
Fragments arranged by size:
2146 1017 787 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 866 962 2731 866
Size: 96 1769 2501
Fragments arranged by size:
2501 1769 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 932 934 584
Size: 348 2 4016
Fragments arranged by size:
4016 348 2
Bpu10I CC'TnA_GC
Cuts at: 1061 1061
Size: 4366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2913 3211 1540
Size: 879 494 298 2695
Fragments arranged by size:
2695 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4070 4070
Size: 4366
BsaAI yAC'GTr
Cuts at: 497 1356 1717 497
Size: 859 361 3146
Fragments arranged by size:
3146 859 361
BsaBI GATnn'nnATC
Cuts at: 6 1878 3917 6
Size: 1872 2039 455
Fragments arranged by size:
2039 1872 455
BsaHI Gr'CG_yC
Cuts at: 1037 1795 2043 2747 4243 1037
Size: 758 248 704 1496 1160
Fragments arranged by size:
1496 1160 758 704 248
BsaWI w'CCGG_w
Cuts at: 1553 2074 2754 3028 3175 3586 1553
Size: 521 680 274 147 411 2333
Fragments arranged by size:
2333 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 431 434 904 931 1171 431
Size: 3 470 27 240 3626
Fragments arranged by size:
3626 470 240 27 3
BseYI C'CCAG_C
Cuts at: 1789 2547 3126 3622 1789
Size: 758 579 496 2533
Fragments arranged by size:
2533 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 1033 728
Size: 305 4061
BsiEI CG_ry'CG
Cuts at: 104 1906 1952 3162 104
Size: 1802 46 1210 1308
Fragments arranged by size:
1802 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1378 2156 2346 3140 1378
Size: 778 190 794 2604
Fragments arranged by size:
2604 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 176 259 266 1378 1989 2082 2156 2346 2408
Size: 83 7 1112 611 93 74 190 62
Cuts at: 2408 3140 176
Size: 732 1402
Fragments arranged by size:
1402 1112 732 611 190 93 83 74
62 7
BspEI T'CCGG_A
Cuts at: 3586 3586
Size: 4366
BspHI T'CATG_A
Cuts at: 3542 3542
Size: 4366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1336 1414 1628 1930 2311 1336
Size: 78 214 302 381 3391
Fragments arranged by size:
3391 381 302 214 78
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4196 4361 50
Size: 2606 54 1486 165 55
Fragments arranged by size:
2606 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 272 2276 272
Size: 2004 2362
BsrFI r'CCGG_y
Cuts at: 2362 2543 2754 2362
Size: 181 211 3974
Fragments arranged by size:
3974 211 181
BssSI C'ACGA_G
Cuts at: 1051 2635 2995 1051
Size: 1584 360 2422
Fragments arranged by size:
2422 1584 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1741 1741
Size: 4366
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4366
BstEII G'GTnAC_C
Cuts at: 537 537
Size: 4366
BstXI CCAn_nnnn'nTGG
Cuts at: 618 1129 618
Size: 511 3855
BstYI r'GATC_y
Cuts at: 1 1389 1856 1879 2214 2460 3474 3560 3572
Size: 1388 467 23 335 246 1014 86 12
Cuts at: 3572 3918 1
Size: 346 449
Fragments arranged by size:
1388 1014 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 125 806 125
Size: 681 3685
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1028 2314 2495 2658 1028
Size: 1286 181 163 2736
Fragments arranged by size:
2736 1286 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1607 1313
Size: 294 4072
ClaI AT'CG_AT
Cuts at: 132 132
Size: 4366
DraI TTT'AAA
Cuts at: 233 1363 233
Size: 1130 3236
DrdI GACnn_nn'nnGTC
Cuts at: 574 1167 2070 2930 574
Size: 593 903 860 2010
Fragments arranged by size:
2010 903 860 593
EaeI y'GGCC_r
Cuts at: 1949 2123 2514 2541 3888 1949
Size: 174 391 27 1347 2427
Fragments arranged by size:
2427 1347 391 174 27
EagI C'GGCC_G
Cuts at: 1949 1949
Size: 4366
EarI CTCTTCn'nnn_
Cuts at: 133 392 824 1232 2387 2597 133
Size: 259 432 408 1155 210 1902
Fragments arranged by size:
1902 1155 432 408 259 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 753 1189 2572 2884 3030 4176 753
Size: 436 1383 312 146 1146 943
Fragments arranged by size:
1383 1146 943 436 312 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 932 934 1024 1256 2189 2621 3370 3638
Size: 348 2 90 232 933 432 749 268
Cuts at: 3638 584
Size: 1312
Fragments arranged by size:
1312 933 749 432 348 268 232 90
2
EcoO109I rG'GnC_Cy
Cuts at: 1503 1503
Size: 4366
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 489 2383 489
Size: 1894 2472
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4366
HaeII r_GCGC'y
Cuts at: 1798 2046 3070 4049 1798
Size: 248 1024 979 2115
Fragments arranged by size:
2115 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 642 674 1027 1059 1201 1216 1233 1248 2206
Size: 32 353 32 142 15 17 15 958
Cuts at: 2206 2236 2238 2268 642
Size: 30 2 30 2740
Fragments arranged by size:
2740 958 353 142 32 32 30 30
17 15 15 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4366
KasI G'GCGC_C
Cuts at: 1794 2042 1794
Size: 248 4118
KpnI G_GTAC'C
Cuts at: 1261 1384 1261
Size: 123 4243
MluI A'CGCG_T
Cuts at: 3799 3799
Size: 4366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3037 3221 3037
Size: 184 4182
MscI TGG'CCA
Cuts at: 2125 2125
Size: 4366
MslI CAynn'nnrTG
Cuts at: 244 499 589 616 1078 1115 2353 2480 244
Size: 255 90 27 462 37 1238 127 2130
Fragments arranged by size:
2130 1238 462 255 127 90 37 27
MspA1I CmG'CkG
Cuts at: 446 1027 1475 1789 2149 3164 3409 446
Size: 581 448 314 360 1015 245 1403
Fragments arranged by size:
1403 1015 581 448 360 314 245
NaeI GCC'GGC
Cuts at: 2545 2545
Size: 4366
NarI GG'CG_CC
Cuts at: 1795 2043 1795
Size: 248 4118
NcoI C'CATG_G
Cuts at: 125 806 125
Size: 681 3685
NgoMIV G'CCGG_C
Cuts at: 2543 2543
Size: 4366
NruI TCG'CGA
Cuts at: 2727 2727
Size: 4366
NspI r_CATG'y
Cuts at: 1114 1417 1917 2826 1114
Size: 303 500 909 2654
Fragments arranged by size:
2654 909 500 303
PciI A'CATG_T
Cuts at: 2822 2822
Size: 4366
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4366
PmlI CAC'GTG
Cuts at: 497 497
Size: 4366
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3505 3537 3505
Size: 32 4334
PstI C_TGCA'G
Cuts at: 722 877 1197 1411 2096 722
Size: 155 320 214 685 2992
Fragments arranged by size:
2992 685 320 214 155
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4366
PvuII CAG'CTG
Cuts at: 1789 2149 1789
Size: 360 4006
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4366
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4366
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4366
SapI GCTCTTCn'nnn_
Cuts at: 133 824 1232 2387 2597 133
Size: 691 408 1155 210 1902
Fragments arranged by size:
1902 1155 691 408 210
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4366
ScaI AGT'ACT
Cuts at: 3883 3883
Size: 4366
SfcI C'TryA_G
Cuts at: 33 327 718 873 1193 1407 2092 3087 3278
Size: 294 391 155 320 214 685 995 191
Cuts at: 3278 33
Size: 1121
Fragments arranged by size:
1121 995 685 391 320 294 214 191
155
SfoI GGC'GCC
Cuts at: 1796 2044 1796
Size: 248 4118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4366
SmaI CCC'GGG
Cuts at: 1386 1386
Size: 4366
SmlI C'TyrA_G
Cuts at: 1519 2434 2928 3190 1519
Size: 915 494 262 2695
Fragments arranged by size:
2695 915 494 262
SnaBI TAC'GTA
Cuts at: 1356 1356
Size: 4366
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4366
SphI G_CATG'C
Cuts at: 1114 1417 1917 1114
Size: 303 500 3563
Fragments arranged by size:
3563 500 303
StyI C'CwwG_G
Cuts at: 125 464 806 1498 1852 125
Size: 339 342 692 354 2639
Fragments arranged by size:
2639 692 354 342 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 497 562 715 808 1947 2080 2539 2750 497
Size: 65 153 93 1139 133 459 211 2113
Fragments arranged by size:
2113 1139 459 211 153 133 93 65
TatI w'GTAC_w
Cuts at: 3881 3881
Size: 4366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 690 722 1081 1113 3883 3915 690
Size: 32 359 32 2770 32 1141
Fragments arranged by size:
2770 1141 359 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4366
XbaI T'CTAG_A
Cuts at: 774 1395 774
Size: 621 3745
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 359 1859 359
Size: 1500 2866
XmaI C'CCGG_G
Cuts at: 1384 1384
Size: 4366
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PciI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BfrBI BglI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NsiI PacI PasI PflMI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SrfI SspI StuI SwaI XhoI XmnI ZraI