Restriction map for
pF1KSDA0607
(Circular) (Six-base) MAPSORT of: pF1KSDA0607.seq Check: 9617 from: 1 to: 5275
pF1KSDA0607 5275 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 259 1206 1386 259
Size: 947 180 4148
Fragments arranged by size:
4148 947 180
AatII G_ACGT'C
Cuts at: 1729 1729
Size: 5275
AccI GT'mk_AC
Cuts at: 2311 2311
Size: 5275
Acc65I G'GTAC_C
Cuts at: 2289 2289
Size: 5275
AclI AA'CG_TT
Cuts at: 5115 5115
Size: 5275
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 940 1816 3098 3530 4279 4547 940
Size: 876 1282 432 749 268 1668
Fragments arranged by size:
1668 1282 876 749 432 268
AfeI AGC'GCT
Cuts at: 187 187
Size: 5275
AflII C'TTAA_G
Cuts at: 1141 1141
Size: 5275
AflIII A'CryG_T
Cuts at: 2625 3731 4708 2625
Size: 1106 977 3192
Fragments arranged by size:
3192 1106 977
AgeI A'CCGG_T
Cuts at: 3663 3663
Size: 5275
AleI CACnn'nnGTG
Cuts at: 1057 1597 1057
Size: 540 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 608 963 608
Size: 355 4920
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1969 2001 1969
Size: 32 5243
AlwNI CAG_nnn'CTG
Cuts at: 549 660 804 1137 1326 2073 2250 2578 4147
Size: 111 144 333 189 747 177 328 1569
Cuts at: 4147 549
Size: 1677
Fragments arranged by size:
1677 1569 747 333 328 189 177 144
111
ApaI G_GGCC'C
Cuts at: 1436 1505 1533 1436
Size: 69 28 5178
Fragments arranged by size:
5178 69 28
ApaLI G'TGCA_C
Cuts at: 4045 4045
Size: 5275
ApoI r'AATT_y
Cuts at: 2277 4628 4884 2277
Size: 2351 256 2668
Fragments arranged by size:
2668 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5275
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5275
AvaI C'yCGr_G
Cuts at: 491 1505 1850 1911 2116 2293 491
Size: 1014 345 61 205 177 3473
Fragments arranged by size:
3473 1014 345 205 177 61
BamHI G'GATC_C
Cuts at: 962 1106 1265 2298 962
Size: 144 159 1033 3939
Fragments arranged by size:
3939 1033 159 144
BanI G'GyrC_C
Cuts at: 354 584 601 1273 1478 2289 2703 2951 2986
Size: 230 17 672 205 811 414 248 35
Cuts at: 2986 354
Size: 2643
Fragments arranged by size:
2643 811 672 414 248 230 205 35
17
BanII G_rGCy'C
Cuts at: 201 722 794 898 1244 1436 1505 1533 1796
Size: 521 72 104 346 192 69 28 263
Cuts at: 1796 1918 1982 2261 2287 3317 201
Size: 122 64 279 26 1030 2159
Fragments arranged by size:
2159 1030 521 346 279 263 192 122
104 72 69 64 28 26
BbeI G_GCGC'C
Cuts at: 358 2707 2955 358
Size: 2349 248 2678
Fragments arranged by size:
2678 2349 248
BbvCI CC'TCA_GC
Cuts at: 770 770
Size: 5275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 214 248 813 847 214
Size: 34 565 34 4642
Fragments arranged by size:
4642 565 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3171 3934 5275 3171
Size: 763 1341 3171
Fragments arranged by size:
3171 1341 763
BclI T'GATC_A
Cuts at: 1517 1679 2793 1517
Size: 162 1114 3999
Fragments arranged by size:
3999 1114 162
BglI GCCn_nnn'nGGC
Cuts at: 406 868 937 1192 1282 1375 1408 1440 406
Size: 462 69 255 90 93 33 32 4241
Fragments arranged by size:
4241 462 255 93 90 69 33 32
BglII A'GATC_T
Cuts at: 2788 4827 2788
Size: 2039 3236
BlpI GC'TnA_GC
Cuts at: 892 2385 892
Size: 1493 3782
Bme1580I G_kGCm'C
Cuts at: 638 836 1054 1436 1505 1533 2898 2991 4049
Size: 198 218 382 69 28 1365 93 1058
Cuts at: 4049 638
Size: 1864
Fragments arranged by size:
1864 1365 1058 382 218 198 93 69
28
BmrI ACTGGGnnnn_n'
Cuts at: 2485 2566 2901 5047 2485
Size: 81 335 2146 2713
Fragments arranged by size:
2713 2146 335 81
BmtI G_CTAG'C
Cuts at: 1936 1936
Size: 5275
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1311 1471 3640 1311
Size: 160 2169 2946
Fragments arranged by size:
2946 2169 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 1060 1808 2062 202
Size: 858 748 254 3415
Fragments arranged by size:
3415 858 748 254
Bpu10I CC'TnA_GC
Cuts at: 770 1813 770
Size: 1043 4232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3328 3822 4120 2449
Size: 879 494 298 3604
Fragments arranged by size:
3604 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1285 4979 1285
Size: 3694 1581
BsaAI yAC'GTr
Cuts at: 1896 2265 2626 1896
Size: 369 361 4545
Fragments arranged by size:
4545 369 361
BsaBI GATnn'nnATC
Cuts at: 6 1516 2787 4826 6
Size: 1510 1271 2039 455
Fragments arranged by size:
2039 1510 1271 455
BsaHI Gr'CG_yC
Cuts at: 355 1691 1726 2704 2952 3656 5152 355
Size: 1336 35 978 248 704 1496 478
Fragments arranged by size:
1496 1336 978 704 478 248 35
BsaWI w'CCGG_w
Cuts at: 2462 2983 3663 3937 4084 4495 2462
Size: 521 680 274 147 411 3242
Fragments arranged by size:
3242 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1969 1999 1969
Size: 30 5245
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1057 1303 1378 1387 1453 1786 2221 1057
Size: 246 75 9 66 333 435 4111
Fragments arranged by size:
4111 435 333 246 75 66 9
BseYI C'CCAG_C
Cuts at: 1391 2698 3456 4035 4531 1391
Size: 1307 758 579 496 2135
Fragments arranged by size:
2135 1307 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 899 1180 1765 899
Size: 281 585 4409
Fragments arranged by size:
4409 585 281
BsiEI CG_ry'CG
Cuts at: 104 2815 2861 4071 104
Size: 2711 46 1210 1308
Fragments arranged by size:
2711 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 794 898 1982 2287 3065 3255 4049 794
Size: 104 1084 305 778 190 794 2020
Fragments arranged by size:
2020 1084 794 778 305 190 104
BsmI GAATG_Cn'
Cuts at: 847 1120 847
Size: 273 5002
BsmBI CGTCTCn'nnnn_
Cuts at: 340 1039 1442 2203 340
Size: 699 403 761 3412
Fragments arranged by size:
3412 761 699 403
Bsp1286I G_dGCh'C
Cuts at: 201 638 722 794 836 898 1054 1244 1436
Size: 437 84 72 42 62 156 190 192
Cuts at: 1436 1505 1533 1796 1918 1982 2261 2287 2898
Size: 69 28 263 122 64 279 26 611
Cuts at: 2898 2991 3065 3255 3317 4049 201
Size: 93 74 190 62 732 1427
Fragments arranged by size:
1427 732 611 437 279 263 192 190
190 156 122 93 84 74 72 69
64 62 62 42 28 26
BspEI T'CCGG_A
Cuts at: 4495 4495
Size: 5275
BspHI T'CATG_A
Cuts at: 1175 1721 4451 1175
Size: 546 2730 1999
Fragments arranged by size:
2730 1999 546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 259 734 1206 1217 1386 1642 1979 2323 2537
Size: 475 472 11 169 256 337 344 214
Cuts at: 2537 2839 3220 259
Size: 302 381 2314
Fragments arranged by size:
2314 475 472 381 344 337 302 256
214 169 11
BsrBI CCG'CTC
Cuts at: 50 521 3565 3619 5105 5270 50
Size: 471 3044 54 1486 165 55
Fragments arranged by size:
3044 1486 471 165 55 54
BsrDI GCAATG_nn'
Cuts at: 590 3185 590
Size: 2595 2680
BsrFI r'CCGG_y
Cuts at: 2008 2168 3271 3452 3663 2008
Size: 160 1103 181 211 3620
Fragments arranged by size:
3620 1103 211 181 160
BssSI C'ACGA_G
Cuts at: 1165 1657 3544 3904 1165
Size: 492 1887 360 2536
Fragments arranged by size:
2536 1887 492 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1137 2073 2650 1137
Size: 936 577 3762
Fragments arranged by size:
3762 936 577
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5275
BstEII G'GTnAC_C
Cuts at: 1074 1662 1074
Size: 588 4687
BstYI r'GATC_y
Cuts at: 1 299 852 962 1106 1265 1511 2298 2765
Size: 298 553 110 144 159 246 787 467
Cuts at: 2765 2788 3123 3369 4383 4469 4481 4827 1
Size: 23 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 787 553 467 449 346 335 298
246 246 159 144 110 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3223 3404 3567 3223
Size: 181 163 4931
Fragments arranged by size:
4931 181 163
BtsI GCAGTG_nn'
Cuts at: 426 1135 1570 1934 2097 2251 2516 426
Size: 709 435 364 163 154 265 3185
Fragments arranged by size:
3185 709 435 364 265 163 154
DraI TTT'AAA
Cuts at: 2272 2272
Size: 5275
DrdI GACnn_nn'nnGTC
Cuts at: 2979 3839 2979
Size: 860 4415
EaeI y'GGCC_r
Cuts at: 434 731 1283 1400 1409 1441 1584 1781 2062
Size: 297 552 117 9 32 143 197 281
Cuts at: 2062 2126 2213 2858 3032 3423 3450 4797 434
Size: 64 87 645 174 391 27 1347 912
Fragments arranged by size:
1347 912 645 552 391 297 281 197
174 143 117 87 64 32 27 9
EagI C'GGCC_G
Cuts at: 2858 2858
Size: 5275
EarI CTCTTCn'nnn_
Cuts at: 1835 1882 3296 3506 1835
Size: 47 1414 210 3604
Fragments arranged by size:
3604 1414 210 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 312 730 3481 3793 3939 5085 312
Size: 418 2751 312 146 1146 502
Fragments arranged by size:
2751 1146 502 418 312 146
EcoICRI GAG'CTC
Cuts at: 792 896 1980 2285 792
Size: 104 1084 305 3782
Fragments arranged by size:
3782 1084 305 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 202 940 1060 1808 1816 2062 3098 3530 4279
Size: 738 120 748 8 246 1036 432 749
Cuts at: 4279 4547 202
Size: 268 930
Fragments arranged by size:
1036 930 749 748 738 432 268 246
120 8
EcoNI CCTnn'n_nnAGG
Cuts at: 205 619 751 1213 1360 1519 205
Size: 414 132 462 147 159 3961
Fragments arranged by size:
3961 462 414 159 147 132
EcoO109I rG'GnC_Cy
Cuts at: 498 577 657 744 1432 1502 1529 1530 2412
Size: 79 80 87 688 70 27 1 882
Cuts at: 2412 498
Size: 3361
Fragments arranged by size:
3361 882 688 87 80 79 70 27
1
EcoRI G'AATT_C
Cuts at: 2277 2277
Size: 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1260 3292 1260
Size: 2032 3243
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5275
HaeII r_GCGC'y
Cuts at: 189 358 2707 2955 3979 4958 189
Size: 169 2349 248 1024 979 506
Fragments arranged by size:
2349 1024 979 506 248 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1282 1314 3115 3145 3147 3177 1282
Size: 32 1801 30 2 30 3380
Fragments arranged by size:
3380 1801 32 30 30 2
HincII GTy'rAC
Cuts at: 2312 2312
Size: 5275
KasI G'GCGC_C
Cuts at: 354 2703 2951 354
Size: 2349 248 2678
Fragments arranged by size:
2678 2349 248
KpnI G_GTAC'C
Cuts at: 2293 2293
Size: 5275
MluI A'CGCG_T
Cuts at: 4708 4708
Size: 5275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 980 3946 4130 980
Size: 2966 184 2125
Fragments arranged by size:
2966 2125 184
MscI TGG'CCA
Cuts at: 1402 1411 1443 1586 1783 3034 1402
Size: 9 32 143 197 1251 3643
Fragments arranged by size:
3643 1251 197 143 32 9
MslI CAynn'nnrTG
Cuts at: 641 1057 1597 1720 3262 3389 641
Size: 416 540 123 1542 127 2527
Fragments arranged by size:
2527 1542 540 416 127 123
MspA1I CmG'CkG
Cuts at: 801 1323 1867 2044 2060 2082 2384 2698 3058
Size: 522 544 177 16 22 302 314 360
Cuts at: 3058 4073 4318 801
Size: 1015 245 1758
Fragments arranged by size:
1758 1015 544 522 360 314 302 245
177 22 16
NaeI GCC'GGC
Cuts at: 3454 3454
Size: 5275
NarI GG'CG_CC
Cuts at: 355 2704 2952 355
Size: 2349 248 2678
Fragments arranged by size:
2678 2349 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5275
NgoMIV G'CCGG_C
Cuts at: 3452 3452
Size: 5275
NheI G'CTAG_C
Cuts at: 1932 1932
Size: 5275
NruI TCG'CGA
Cuts at: 3636 3636
Size: 5275
NspI r_CATG'y
Cuts at: 1998 2326 2826 3735 1998
Size: 328 500 909 3538
Fragments arranged by size:
3538 909 500 328
PasI CC'CwG_GG
Cuts at: 1429 1788 1429
Size: 359 4916
PciI A'CATG_T
Cuts at: 3731 3731
Size: 5275
PflMI CCAn_nnn'nTGG
Cuts at: 660 1398 660
Size: 738 4537
PfoI T'CCnGG_A
Cuts at: 979 1109 979
Size: 130 5145
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5275
PmlI CAC'GTG
Cuts at: 1896 1896
Size: 5275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1969 2001 4414 4446 1969
Size: 32 2413 32 2798
Fragments arranged by size:
2798 2413 32 32
PpuMI rG'GwC_Cy
Cuts at: 498 498
Size: 5275
PspOMI G'GGCC_C
Cuts at: 1432 1501 1529 1432
Size: 69 28 5178
Fragments arranged by size:
5178 69 28
PstI C_TGCA'G
Cuts at: 421 860 1217 1562 1631 1976 2198 2320 3005
Size: 439 357 345 69 345 222 122 685
Cuts at: 3005 421
Size: 2691
Fragments arranged by size:
2691 685 439 357 345 345 222 122
69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5275
PvuII CAG'CTG
Cuts at: 801 1323 1867 2082 2698 3058 801
Size: 522 544 215 616 360 3018
Fragments arranged by size:
3018 616 544 522 360 215
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5275
SacI G_AGCT'C
Cuts at: 794 898 1982 2287 794
Size: 104 1084 305 3782
Fragments arranged by size:
3782 1084 305 104
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5275
SanDI GG'GwC_CC
Cuts at: 498 498
Size: 5275
SapI GCTCTTCn'nnn_
Cuts at: 1835 3296 3506 1835
Size: 1461 210 3604
Fragments arranged by size:
3604 1461 210
SbfI CC_TGCA'GG
Cuts at: 1976 2320 1976
Size: 344 4931
ScaI AGT'ACT
Cuts at: 4792 4792
Size: 5275
SfcI C'TryA_G
Cuts at: 33 417 557 856 1213 1558 1627 1972 2194
Size: 384 140 299 357 345 69 345 222
Cuts at: 2194 2316 3001 3996 4187 33
Size: 122 685 995 191 1121
Fragments arranged by size:
1121 995 685 384 357 345 345 299
222 191 140 122 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 406 1375 1408 1440 406
Size: 969 33 32 4241
Fragments arranged by size:
4241 969 33 32
SfoI GGC'GCC
Cuts at: 356 2705 2953 356
Size: 2349 248 2678
Fragments arranged by size:
2678 2349 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5275
SmaI CCC'GGG
Cuts at: 493 1507 1913 2295 493
Size: 1014 406 382 3473
Fragments arranged by size:
3473 1014 406 382
SmlI C'TyrA_G
Cuts at: 1141 2428 3343 3837 4099 1141
Size: 1287 915 494 262 2317
Fragments arranged by size:
2317 1287 915 494 262
SnaBI TAC'GTA
Cuts at: 2265 2265
Size: 5275
SpeI A'CTAG_T
Cuts at: 67 1740 67
Size: 1673 3602
SphI G_CATG'C
Cuts at: 1998 2326 2826 1998
Size: 328 500 4447
Fragments arranged by size:
4447 500 328
StuI AGG'CCT
Cuts at: 2002 2002
Size: 5275
StyI C'CwwG_G
Cuts at: 125 254 347 2237 2407 2761 125
Size: 129 93 1890 170 354 2639
Fragments arranged by size:
2639 1890 354 170 129 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 382 585 1261 2856 2989 3448 3659 382
Size: 203 676 1595 133 459 211 1998
Fragments arranged by size:
1998 1595 676 459 211 203 133
TatI w'GTAC_w
Cuts at: 4790 4790
Size: 5275
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1257 1289 4792 4824 1257
Size: 32 3503 32 1708
Fragments arranged by size:
3503 1708 32 32
Tth111I GACn'n_nGTC
Cuts at: 3070 3070
Size: 5275
XbaI T'CTAG_A
Cuts at: 2304 2304
Size: 5275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 2768 1436
Size: 1332 3943
XmaI C'CCGG_G
Cuts at: 491 1505 1911 2293 491
Size: 1014 406 382 3473
Fragments arranged by size:
3473 1014 406 382
ZraI GAC'GTC
Cuts at: 1727 1727
Size: 5275
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NruI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AhdI AscI AvrII BaeI BbsI BfrBI BmgBI BsiWI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NsiI PacI PshAI PsiI PspXI PsrI SacII SexAI
SgrAI SrfI SspI SwaI XhoI XmnI