Restriction map for
pF1KSDA0604
(Circular) (Six-base) MAPSORT of: pF1KSDA0604.seq Check: 2062 from: 1 to: 5434
pF1KSDA0604 5434 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 307 412 605 1017 1230 307
Size: 105 193 412 213 4511
Fragments arranged by size:
4511 412 213 193 105
AccI GT'mk_AC
Cuts at: 830 2470 830
Size: 1640 3794
Acc65I G'GTAC_C
Cuts at: 2448 2448
Size: 5434
AclI AA'CG_TT
Cuts at: 5274 5274
Size: 5434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 610 1709 2155 3257 3689 4438 4706 610
Size: 1099 446 1102 432 749 268 1338
Fragments arranged by size:
1338 1102 1099 749 446 432 268
AfeI AGC'GCT
Cuts at: 652 652
Size: 5434
AflIII A'CryG_T
Cuts at: 1709 2784 3890 4867 1709
Size: 1075 1106 977 2276
Fragments arranged by size:
2276 1106 1075 977
AgeI A'CCGG_T
Cuts at: 3822 3822
Size: 5434
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1259 1291 1259
Size: 32 5402
AlwNI CAG_nnn'CTG
Cuts at: 147 404 566 708 1032 1134 2103 2193 2737
Size: 257 162 142 324 102 969 90 544
Cuts at: 2737 4306 147
Size: 1569 1275
Fragments arranged by size:
1569 1275 969 544 324 257 162 142
102 90
ApaI G_GGCC'C
Cuts at: 755 755
Size: 5434
ApaLI G'TGCA_C
Cuts at: 4204 4204
Size: 5434
ApoI r'AATT_y
Cuts at: 1682 1716 2436 4787 5043 1682
Size: 34 720 2351 256 2073
Fragments arranged by size:
2351 2073 720 256 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 5434
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5434
AvaI C'yCGr_G
Cuts at: 208 905 2452 208
Size: 697 1547 3190
Fragments arranged by size:
3190 1547 697
AvrII C'CTAG_G
Cuts at: 366 366
Size: 5434
BamHI G'GATC_C
Cuts at: 2457 2457
Size: 5434
BanI G'GyrC_C
Cuts at: 319 1137 2448 2862 3110 3145 319
Size: 818 1311 414 248 35 2608
Fragments arranged by size:
2608 1311 818 414 248 35
BanII G_rGCy'C
Cuts at: 472 702 755 893 1253 1649 2210 2278 2446
Size: 230 53 138 360 396 561 68 168
Cuts at: 2446 3476 472
Size: 1030 2430
Fragments arranged by size:
2430 1030 561 396 360 230 168 138
68 53
BbeI G_GCGC'C
Cuts at: 1141 2866 3114 1141
Size: 1725 248 3461
Fragments arranged by size:
3461 1725 248
BbsI GAAGACnn'nnnn_
Cuts at: 206 649 1579 1833 206
Size: 443 930 254 3807
Fragments arranged by size:
3807 930 443 254
BciVI GTATCCnnnnn_n'
Cuts at: 3330 4093 5434 3330
Size: 763 1341 3330
Fragments arranged by size:
3330 1341 763
BclI T'GATC_A
Cuts at: 1511 2952 1511
Size: 1441 3993
BglI GCCn_nnn'nGGC
Cuts at: 534 1404 1998 2381 534
Size: 870 594 383 3587
Fragments arranged by size:
3587 870 594 383
BglII A'GATC_T
Cuts at: 1097 2947 4986 1097
Size: 1850 2039 1545
Fragments arranged by size:
2039 1850 1545
BlpI GC'TnA_GC
Cuts at: 635 2544 635
Size: 1909 3525
Bme1580I G_kGCm'C
Cuts at: 755 1070 2335 3057 3150 4208 755
Size: 315 1265 722 93 1058 1981
Fragments arranged by size:
1981 1265 1058 722 315 93
BmrI ACTGGGnnnn_n'
Cuts at: 985 2644 2725 3060 5206 985
Size: 1659 81 335 2146 1213
Fragments arranged by size:
2146 1659 1213 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3799 3799
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 466 606 1054 1384 1663 322
Size: 144 140 448 330 279 4093
Fragments arranged by size:
4093 448 330 279 144 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1177 1305 2608 3487 3981 4279 1177
Size: 128 1303 879 494 298 2332
Fragments arranged by size:
2332 1303 879 494 298 128
BsaI GGTCTCn'nnnn_
Cuts at: 205 380 707 1360 1769 5138 205
Size: 175 327 653 409 3369 501
Fragments arranged by size:
3369 653 501 409 327 175
BsaAI yAC'GTr
Cuts at: 2424 2785 2424
Size: 361 5073
BsaBI GATnn'nnATC
Cuts at: 6 2946 4985 6
Size: 2940 2039 455
Fragments arranged by size:
2940 2039 455
BsaHI Gr'CG_yC
Cuts at: 242 1138 1433 2863 3111 3815 5311 242
Size: 896 295 1430 248 704 1496 365
Fragments arranged by size:
1496 1430 896 704 365 295 248
BsaWI w'CCGG_w
Cuts at: 1523 2024 2621 3142 3822 4096 4243 4654 1523
Size: 501 597 521 680 274 147 411 2303
Fragments arranged by size:
2303 680 597 521 501 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 478 508 2038 2068 478
Size: 30 1530 30 3844
Fragments arranged by size:
3844 1530 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 703 1105 1552 2197 703
Size: 402 447 645 3940
Fragments arranged by size:
3940 645 447 402
BseYI C'CCAG_C
Cuts at: 147 693 701 987 1768 2103 2328 2857 3615
Size: 546 8 286 781 335 225 529 758
Cuts at: 3615 4194 4690 147
Size: 579 496 891
Fragments arranged by size:
891 781 758 579 546 529 496 335
286 225 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 1331 323
Size: 1008 4426
BsiEI CG_ry'CG
Cuts at: 104 2094 2974 3020 4230 104
Size: 1990 880 46 1210 1308
Fragments arranged by size:
1990 1308 1210 880 46
BsiHKAI G_wGCw'C
Cuts at: 947 1253 2257 2278 2446 3224 3414 4208 947
Size: 306 1004 21 168 778 190 794 2173
Fragments arranged by size:
2173 1004 794 778 306 190 168 21
BsmI GAATG_Cn'
Cuts at: 422 1812 422
Size: 1390 4044
Bsp1286I G_dGCh'C
Cuts at: 472 702 755 893 947 1070 1253 1649 2210
Size: 230 53 138 54 123 183 396 561
Cuts at: 2210 2257 2278 2335 2446 3057 3150 3224 3414
Size: 47 21 57 111 611 93 74 190
Cuts at: 3414 3476 4208 472
Size: 62 732 1698
Fragments arranged by size:
1698 732 611 561 396 230 190 183
138 123 111 93 74 62 57 54
53 47 21
BspEI T'CCGG_A
Cuts at: 1523 2024 4654 1523
Size: 501 2630 2303
Fragments arranged by size:
2630 2303 501
BspHI T'CATG_A
Cuts at: 4610 4610
Size: 5434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 307 412 605 1017 1230 1420 1994 2482 2696
Size: 105 193 412 213 190 574 488 214
Cuts at: 2696 2998 3379 307
Size: 302 381 2362
Fragments arranged by size:
2362 574 488 412 381 302 214 213
193 190 105
BsrBI CCG'CTC
Cuts at: 50 3724 3778 5264 5429 50
Size: 3674 54 1486 165 55
Fragments arranged by size:
3674 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 870 2114 3344 870
Size: 1244 1230 2960
Fragments arranged by size:
2960 1244 1230
BsrFI r'CCGG_y
Cuts at: 1481 3430 3611 3822 1481
Size: 1949 181 211 3093
Fragments arranged by size:
3093 1949 211 181
BsrGI T'GTAC_A
Cuts at: 1721 1721
Size: 5434
BssSI C'ACGA_G
Cuts at: 1008 2281 3703 4063 1008
Size: 1273 1422 360 2379
Fragments arranged by size:
2379 1422 1273 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 2193 2809 1029
Size: 1164 616 3654
Fragments arranged by size:
3654 1164 616
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5434
BstEII G'GTnAC_C
Cuts at: 2292 2292
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 252 1656 1743 2074 252
Size: 1404 87 331 3612
Fragments arranged by size:
3612 1404 331 87
BstYI r'GATC_y
Cuts at: 1 1097 2457 2924 2947 3282 3528 4542 4628
Size: 1096 1360 467 23 335 246 1014 86
Cuts at: 4628 4640 4986 1
Size: 12 346 449
Fragments arranged by size:
1360 1096 1014 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 245 1145 2201 245
Size: 900 1056 3478
Fragments arranged by size:
3478 1056 900
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3382 3563 3726 3382
Size: 181 163 5090
Fragments arranged by size:
5090 181 163
BtsI GCAGTG_nn'
Cuts at: 340 633 947 2675 340
Size: 293 314 1728 3099
Fragments arranged by size:
3099 1728 314 293
DraI TTT'AAA
Cuts at: 2431 2431
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 3138 3998 3138
Size: 860 4574
EaeI y'GGCC_r
Cuts at: 1052 2091 3017 3191 3582 3609 4956 1052
Size: 1039 926 174 391 27 1347 1530
Fragments arranged by size:
1530 1347 1039 926 391 174 27
EagI C'GGCC_G
Cuts at: 2091 3017 2091
Size: 926 4508
EarI CTCTTCn'nnn_
Cuts at: 1465 2230 3455 3665 1465
Size: 765 1225 210 3234
Fragments arranged by size:
3234 1225 765 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3640 3952 4098 5244 3640
Size: 312 146 1146 3830
Fragments arranged by size:
3830 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1251 2276 2444 1251
Size: 1025 168 4241
Fragments arranged by size:
4241 1025 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 466 606 610 1054 1384 1663 1709 2155
Size: 144 140 4 444 330 279 46 446
Cuts at: 2155 3257 3689 4438 4706 322
Size: 1102 432 749 268 1050
Fragments arranged by size:
1102 1050 749 446 444 432 330 279
268 144 140 46 4
EcoNI CCTnn'n_nnAGG
Cuts at: 412 1008 412
Size: 596 4838
EcoO109I rG'GnC_Cy
Cuts at: 230 751 752 800 1454 1542 1863 1893 2571
Size: 521 1 48 654 88 321 30 678
Cuts at: 2571 230
Size: 3093
Fragments arranged by size:
3093 678 654 521 321 88 48 30
1
EcoRI G'AATT_C
Cuts at: 2436 2436
Size: 5434
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3451 3451
Size: 5434
FspI TGC'GCA
Cuts at: 3213 3213
Size: 5434
HaeII r_GCGC'y
Cuts at: 654 1141 2866 3114 4138 5117 654
Size: 487 1725 248 1024 979 971
Fragments arranged by size:
1725 1024 979 971 487 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1150 1182 3274 3304 3306 3336 1150
Size: 32 2092 30 2 30 3248
Fragments arranged by size:
3248 2092 32 30 30 2
HincII GTy'rAC
Cuts at: 1937 2471 1937
Size: 534 4900
KasI G'GCGC_C
Cuts at: 1137 2862 3110 1137
Size: 1725 248 3461
Fragments arranged by size:
3461 1725 248
KpnI G_GTAC'C
Cuts at: 2452 2452
Size: 5434
MluI A'CGCG_T
Cuts at: 4867 4867
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 718 881 1019 1445 1846 2366 4105 4289 718
Size: 163 138 426 401 520 1739 184 1863
Fragments arranged by size:
1863 1739 520 426 401 184 163 138
MscI TGG'CCA
Cuts at: 1054 3193 1054
Size: 2139 3295
MslI CAynn'nnrTG
Cuts at: 250 427 1063 1915 3421 3548 250
Size: 177 636 852 1506 127 2136
Fragments arranged by size:
2136 1506 852 636 177 127
MspA1I CmG'CkG
Cuts at: 279 300 1050 1080 1554 1849 2406 2543 2857
Size: 21 750 30 474 295 557 137 314
Cuts at: 2857 3217 4232 4477 279
Size: 360 1015 245 1236
Fragments arranged by size:
1236 1015 750 557 474 360 314 295
245 137 30 21
NaeI GCC'GGC
Cuts at: 3613 3613
Size: 5434
NarI GG'CG_CC
Cuts at: 1138 2863 3111 1138
Size: 1725 248 3461
Fragments arranged by size:
3461 1725 248
NcoI C'CATG_G
Cuts at: 245 1145 245
Size: 900 4534
NgoMIV G'CCGG_C
Cuts at: 3611 3611
Size: 5434
NruI TCG'CGA
Cuts at: 3795 3795
Size: 5434
NspI r_CATG'y
Cuts at: 1713 1945 2485 2985 3894 1713
Size: 232 540 500 909 3253
Fragments arranged by size:
3253 909 540 500 232
PasI CC'CwG_GG
Cuts at: 755 1545 2398 755
Size: 790 853 3791
Fragments arranged by size:
3791 853 790
PciI A'CATG_T
Cuts at: 1709 3890 1709
Size: 2181 3253
PflMI CCAn_nnn'nTGG
Cuts at: 1151 2103 1151
Size: 952 4482
PfoI T'CCnGG_A
Cuts at: 443 1640 443
Size: 1197 4237
PmeI GTTT'AAAC
Cuts at: 2431 2431
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4573 4605 4573
Size: 32 5402
PpuMI rG'GwC_Cy
Cuts at: 800 1454 800
Size: 654 4780
PspOMI G'GGCC_C
Cuts at: 751 751
Size: 5434
PstI C_TGCA'G
Cuts at: 143 1130 1862 2479 3164 143
Size: 987 732 617 685 2413
Fragments arranged by size:
2413 987 732 685 617
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5434
PvuII CAG'CTG
Cuts at: 279 300 1050 1554 2406 2857 3217 279
Size: 21 750 504 852 451 360 2496
Fragments arranged by size:
2496 852 750 504 451 360 21
RsrII CG'GwC_CG
Cuts at: 1526 2259 3627 1526
Size: 733 1368 3333
Fragments arranged by size:
3333 1368 733
SacI G_AGCT'C
Cuts at: 1253 2278 2446 1253
Size: 1025 168 4241
Fragments arranged by size:
4241 1025 168
SalI G'TCGA_C
Cuts at: 2469 2469
Size: 5434
SanDI GG'GwC_CC
Cuts at: 1454 1454
Size: 5434
SapI GCTCTTCn'nnn_
Cuts at: 2230 3455 3665 2230
Size: 1225 210 3999
Fragments arranged by size:
3999 1225 210
SbfI CC_TGCA'GG
Cuts at: 1862 2479 1862
Size: 617 4817
ScaI AGT'ACT
Cuts at: 4951 4951
Size: 5434
SexAI A'CCwGG_T
Cuts at: 1301 2066 1301
Size: 765 4669
SfcI C'TryA_G
Cuts at: 33 139 670 804 1126 1858 2475 3160 4155
Size: 106 531 134 322 732 617 685 995
Cuts at: 4155 4346 33
Size: 191 1121
Fragments arranged by size:
1121 995 732 685 617 531 322 191
134 106
SfoI GGC'GCC
Cuts at: 1139 2864 3112 1139
Size: 1725 248 3461
Fragments arranged by size:
3461 1725 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5434
SmaI CCC'GGG
Cuts at: 2454 2454
Size: 5434
SmlI C'TyrA_G
Cuts at: 1156 1320 2587 3502 3996 4258 1156
Size: 164 1267 915 494 262 2332
Fragments arranged by size:
2332 1267 915 494 262 164
SnaBI TAC'GTA
Cuts at: 2424 2424
Size: 5434
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5434
SphI G_CATG'C
Cuts at: 1945 2485 2985 1945
Size: 540 500 4394
Fragments arranged by size:
4394 540 500
StuI AGG'CCT
Cuts at: 418 418
Size: 5434
StyI C'CwwG_G
Cuts at: 245 366 1145 1437 1589 1736 1889 1956 2566
Size: 121 779 292 152 147 153 67 610
Cuts at: 2566 2920 245
Size: 354 2759
Fragments arranged by size:
2759 779 610 354 292 153 152 147
121 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 473 979 1346 2247 2311 3015 3148 3607 3818
Size: 506 367 901 64 704 133 459 211
Cuts at: 3818 473
Size: 2089
Fragments arranged by size:
2089 901 704 506 459 367 211 133
64
TatI w'GTAC_w
Cuts at: 173 1721 2054 4949 173
Size: 1548 333 2895 658
Fragments arranged by size:
2895 1548 658 333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4951 4983 4951
Size: 32 5402
Tth111I GACn'n_nGTC
Cuts at: 3229 3229
Size: 5434
XbaI T'CTAG_A
Cuts at: 2463 2463
Size: 5434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 496 2927 496
Size: 2431 3003
XmaI C'CCGG_G
Cuts at: 2452 2452
Size: 5434
XmnI GAAnn'nnTTC
Cuts at: 553 553
Size: 5434
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI BaeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SrfI SspI SwaI XhoI ZraI