Restriction map for
pF1KSDA0587
(Circular) (Six-base) MAPSORT of: pF1KSDA0587.seq Check: 9859 from: 1 to: 6539
pF1KSDA0587 6539 bp
With 182 enzymes: SgfI *
June 20, 2008 10:34 ..
AccI GT'mk_AC
Cuts at: 3339 3500 3577 3339
Size: 161 77 6301
Fragments arranged by size:
6301 161 77
Acc65I G'GTAC_C
Cuts at: 3555 3555
Size: 6539
AclI AA'CG_TT
Cuts at: 6379 6379
Size: 6539
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 1523 2336 4364 4796 5543 5811 1213
Size: 310 813 2028 432 747 268 1941
Fragments arranged by size:
2028 1941 813 747 432 310 268
AflII C'TTAA_G
Cuts at: 2740 2740
Size: 6539
AflIII A'CryG_T
Cuts at: 1395 3891 4995 5972 1395
Size: 2496 1104 977 1962
Fragments arranged by size:
2496 1962 1104 977
AgeI A'CCGG_T
Cuts at: 4927 4927
Size: 6539
AlwNI CAG_nnn'CTG
Cuts at: 162 3844 5411 162
Size: 3682 1567 1290
Fragments arranged by size:
3682 1567 1290
ApaLI G'TGCA_C
Cuts at: 845 5309 845
Size: 4464 2075
ApoI r'AATT_y
Cuts at: 316 331 448 727 3313 3543 5892 6148 316
Size: 15 117 279 2586 230 2349 256 707
Fragments arranged by size:
2586 2349 707 279 256 230 117 15
AseI AT'TA_AT
Cuts at: 20 1106 1366 20
Size: 1086 260 5193
Fragments arranged by size:
5193 1086 260
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6539
AvaI C'yCGr_G
Cuts at: 3559 3559
Size: 6539
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 568 601 568
Size: 33 6506
BamHI G'GATC_C
Cuts at: 956 3564 956
Size: 2608 3931
BanI G'GyrC_C
Cuts at: 3555 3969 4217 4252 3555
Size: 414 248 35 5842
Fragments arranged by size:
5842 414 248 35
BanII G_rGCy'C
Cuts at: 3553 4583 3553
Size: 1030 5509
BbeI G_GCGC'C
Cuts at: 3973 4221 3973
Size: 248 6291
BbsI GAAGACnn'nnnn_
Cuts at: 492 1073 3305 492
Size: 581 2232 3726
Fragments arranged by size:
3726 2232 581
BciVI GTATCCnnnnn_n'
Cuts at: 4437 5198 6539 4437
Size: 761 1341 4437
Fragments arranged by size:
4437 1341 761
BclI T'GATC_A
Cuts at: 1218 4059 1218
Size: 2841 3698
BfrBI ATG'CAT
Cuts at: 3487 3487
Size: 6539
BglII A'GATC_T
Cuts at: 1183 1755 4054 6091 1183
Size: 572 2299 2037 1631
Fragments arranged by size:
2299 2037 1631 572
BlpI GC'TnA_GC
Cuts at: 3651 3651
Size: 6539
Bme1580I G_kGCm'C
Cuts at: 849 4164 4257 5313 849
Size: 3315 93 1056 2075
Fragments arranged by size:
3315 2075 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 143 819 3751 3832 4167 6311 143
Size: 676 2932 81 335 2144 371
Fragments arranged by size:
2932 2144 676 371 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1839 4906 1839
Size: 3067 3472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2377 3318 2377
Size: 941 5598
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3715 4594 5086 5384 3715
Size: 879 492 298 4870
Fragments arranged by size:
4870 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 254 6243 254
Size: 5989 550
BsaAI yAC'GTr
Cuts at: 1434 3531 3892 1434
Size: 2097 361 4081
Fragments arranged by size:
4081 2097 361
BsaBI GATnn'nnATC
Cuts at: 6 2835 4053 6090 6
Size: 2829 1218 2037 455
Fragments arranged by size:
2829 2037 1218 455
BsaHI Gr'CG_yC
Cuts at: 197 3970 4218 4920 6416 197
Size: 3773 248 702 1496 320
Fragments arranged by size:
3773 1496 702 320 248
BsaWI w'CCGG_w
Cuts at: 3728 4249 4927 5201 5348 5759 3728
Size: 521 678 274 147 411 4508
Fragments arranged by size:
4508 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 3964 4722 5299 5795 3964
Size: 758 577 496 4708
Fragments arranged by size:
4708 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1172 1352 1456 1501 2168 2434 2801 1172
Size: 180 104 45 667 266 367 4910
Fragments arranged by size:
4910 667 367 266 180 104 45
BsiEI CG_ry'CG
Cuts at: 104 192 4081 4127 5335 104
Size: 88 3889 46 1208 1308
Fragments arranged by size:
3889 1308 1208 88 46
BsiHKAI G_wGCw'C
Cuts at: 849 1395 3553 4331 4521 5313 849
Size: 546 2158 778 190 792 2075
Fragments arranged by size:
2158 2075 792 778 546 190
BsmI GAATG_Cn'
Cuts at: 1126 1126
Size: 6539
Bsp1286I G_dGCh'C
Cuts at: 849 1395 3553 4164 4257 4331 4521 4583 5313
Size: 546 2158 611 93 74 190 62 730
Cuts at: 5313 849
Size: 2075
Fragments arranged by size:
2158 2075 730 611 546 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5759 5759
Size: 6539
BspHI T'CATG_A
Cuts at: 5715 5715
Size: 6539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3589 3803 4105 4486 3589
Size: 214 302 381 5642
Fragments arranged by size:
5642 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4831 4885 6369 6534 50
Size: 4781 54 1484 165 55
Fragments arranged by size:
4781 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 920 1355 2553 2579 3014 3216 3231 4451 920
Size: 435 1198 26 435 202 15 1220 3008
Fragments arranged by size:
3008 1220 1198 435 435 202 26 15
BsrFI r'CCGG_y
Cuts at: 4537 4718 4927 4537
Size: 181 209 6149
Fragments arranged by size:
6149 209 181
BsrGI T'GTAC_A
Cuts at: 3417 3417
Size: 6539
BssSI C'ACGA_G
Cuts at: 1477 2255 4810 5168 1477
Size: 778 2555 358 2848
Fragments arranged by size:
2848 2555 778 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3493 3916 3493
Size: 423 6116
BstBI TT'CG_AA
Cuts at: 110 4900 110
Size: 4790 1749
BstYI r'GATC_y
Cuts at: 1 956 1183 1755 3444 3564 4031 4054 4389
Size: 955 227 572 1689 120 467 23 335
Cuts at: 4389 4635 5647 5733 5745 6091 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1689 1012 955 572 467 449 346 335
246 227 120 86 23 12
BtgI C'CryG_G
Cuts at: 951 951
Size: 6539
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4489 4670 4833 4489
Size: 181 163 6195
Fragments arranged by size:
6195 181 163
BtsI GCAGTG_nn'
Cuts at: 2069 2180 3782 2069
Size: 111 1602 4826
Fragments arranged by size:
4826 1602 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2210 2245 2210
Size: 35 6504
DraI TTT'AAA
Cuts at: 710 2826 3538 710
Size: 2116 712 3711
Fragments arranged by size:
3711 2116 712
DrdI GACnn_nn'nnGTC
Cuts at: 196 4245 5103 196
Size: 4049 858 1632
Fragments arranged by size:
4049 1632 858
EaeI y'GGCC_r
Cuts at: 678 1961 2550 3173 3239 3351 4124 4298 4689
Size: 1283 589 623 66 112 773 174 391
Cuts at: 4689 4716 6061 678
Size: 27 1345 1156
Fragments arranged by size:
1345 1283 1156 773 623 589 391 174
112 66 27
EagI C'GGCC_G
Cuts at: 4124 4124
Size: 6539
EarI CTCTTCn'nnn_
Cuts at: 776 1007 1321 1895 2851 3110 4562 4772 776
Size: 231 314 574 956 259 1452 210 2543
Fragments arranged by size:
2543 1452 956 574 314 259 231 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 181 4747 5057 5203 6349 181
Size: 4566 310 146 1146 371
Fragments arranged by size:
4566 1146 371 310 146
EcoICRI GAG'CTC
Cuts at: 3551 3551
Size: 6539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1213 1523 2336 2377 3318 4364 4796 5543 5811
Size: 310 813 41 941 1046 432 747 268
Cuts at: 5811 1213
Size: 1941
Fragments arranged by size:
1941 1046 941 813 747 432 310 268
41
EcoO109I rG'GnC_Cy
Cuts at: 1237 3678 1237
Size: 2441 4098
EcoRI G'AATT_C
Cuts at: 3543 3543
Size: 6539
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 558 1031 2929 3322 4558 558
Size: 473 1898 393 1236 2539
Fragments arranged by size:
2539 1898 1236 473 393
FspI TGC'GCA
Cuts at: 4320 4320
Size: 6539
HaeII r_GCGC'y
Cuts at: 3325 3973 4221 5243 6222 3325
Size: 648 248 1022 979 3642
Fragments arranged by size:
3642 1022 979 648 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2259 2291 2911 2943 3013 3045 4381 4411 4413
Size: 32 620 32 70 32 1336 30 2
Cuts at: 4413 4443 2259
Size: 30 4355
Fragments arranged by size:
4355 1336 620 70 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1536 2774 2844 3578 1536
Size: 1238 70 734 4497
Fragments arranged by size:
4497 1238 734 70
HpaI GTT'AAC
Cuts at: 1536 2774 1536
Size: 1238 5301
KasI G'GCGC_C
Cuts at: 3969 4217 3969
Size: 248 6291
KpnI G_GTAC'C
Cuts at: 3559 3559
Size: 6539
MfeI C'AATT_G
Cuts at: 1841 2300 2957 1841
Size: 459 657 5423
Fragments arranged by size:
5423 657 459
MluI A'CGCG_T
Cuts at: 5972 5972
Size: 6539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 902 5210 5394 902
Size: 4308 184 2047
Fragments arranged by size:
4308 2047 184
MscI TGG'CCA
Cuts at: 680 1963 2552 3175 3241 3353 4300 680
Size: 1283 589 623 66 112 947 2919
Fragments arranged by size:
2919 1283 947 623 589 112 66
MslI CAynn'nnrTG
Cuts at: 464 4528 4655 464
Size: 4064 127 2348
Fragments arranged by size:
4064 2348 127
MspA1I CmG'CkG
Cuts at: 805 3034 3650 3964 4324 5337 5582 805
Size: 2229 616 314 360 1013 245 1762
Fragments arranged by size:
2229 1762 1013 616 360 314 245
NaeI GCC'GGC
Cuts at: 4720 4720
Size: 6539
NarI GG'CG_CC
Cuts at: 3970 4218 3970
Size: 248 6291
NcoI C'CATG_G
Cuts at: 951 951
Size: 6539
NdeI CA'TA_TG
Cuts at: 2679 2679
Size: 6539
NgoMIV G'CCGG_C
Cuts at: 4718 4718
Size: 6539
NsiI A_TGCA'T
Cuts at: 3489 3489
Size: 6539
NspI r_CATG'y
Cuts at: 207 257 1147 1320 1399 3592 4092 4999 207
Size: 50 890 173 79 2193 500 907 1747
Fragments arranged by size:
2193 1747 907 890 500 173 79 50
PciI A'CATG_T
Cuts at: 1395 4995 1395
Size: 3600 2939
PflMI CCAn_nnn'nTGG
Cuts at: 676 676
Size: 6539
PmeI GTTT'AAAC
Cuts at: 3538 3538
Size: 6539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5678 5710 5678
Size: 32 6507
PpuMI rG'GwC_Cy
Cuts at: 1237 1237
Size: 6539
PsiI TTA'TAA
Cuts at: 556 556
Size: 6539
PstI C_TGCA'G
Cuts at: 146 1065 3267 3586 4271 146
Size: 919 2202 319 685 2414
Fragments arranged by size:
2414 2202 919 685 319
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6539
PvuII CAG'CTG
Cuts at: 805 3034 3964 4324 805
Size: 2229 930 360 3020
Fragments arranged by size:
3020 2229 930 360
RsrII CG'GwC_CG
Cuts at: 4734 4734
Size: 6539
SacI G_AGCT'C
Cuts at: 3553 3553
Size: 6539
SalI G'TCGA_C
Cuts at: 3576 3576
Size: 6539
SapI GCTCTTCn'nnn_
Cuts at: 1007 1895 4562 4772 1007
Size: 888 2667 210 2774
Fragments arranged by size:
2774 2667 888 210
SbfI CC_TGCA'GG
Cuts at: 3586 3586
Size: 6539
ScaI AGT'ACT
Cuts at: 6056 6056
Size: 6539
SexAI A'CCwGG_T
Cuts at: 2121 2121
Size: 6539
SfcI C'TryA_G
Cuts at: 33 142 341 379 974 1061 2597 3206 3263
Size: 109 199 38 595 87 1536 609 57
Cuts at: 3263 3582 4267 5260 5451 33
Size: 319 685 993 191 1121
Fragments arranged by size:
1536 1121 993 685 609 595 319 199
191 109 87 57 38
SfoI GGC'GCC
Cuts at: 3971 4219 3971
Size: 248 6291
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6539
SmaI CCC'GGG
Cuts at: 3561 3561
Size: 6539
SmlI C'TyrA_G
Cuts at: 2740 3694 4609 5101 5363 2740
Size: 954 915 492 262 3916
Fragments arranged by size:
3916 954 915 492 262
SnaBI TAC'GTA
Cuts at: 3531 3531
Size: 6539
SpeI A'CTAG_T
Cuts at: 67 1633 67
Size: 1566 4973
SphI G_CATG'C
Cuts at: 207 3592 4092 207
Size: 3385 500 2654
Fragments arranged by size:
3385 2654 500
SspI AAT'ATT
Cuts at: 1370 2263 2632 1370
Size: 893 369 5277
Fragments arranged by size:
5277 893 369
StyI C'CwwG_G
Cuts at: 266 675 951 1241 1727 2983 3673 4027 266
Size: 409 276 290 486 1256 690 354 2778
Fragments arranged by size:
2778 1256 690 486 409 354 290 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 4122 4255 4714 4923 4122
Size: 133 459 209 5738
Fragments arranged by size:
5738 459 209 133
TatI w'GTAC_w
Cuts at: 853 3194 3417 6054 853
Size: 2341 223 2637 1338
Fragments arranged by size:
2637 2341 1338 223
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 240 272 6056 6088 240
Size: 32 5784 32 691
Fragments arranged by size:
5784 691 32 32
Tth111I GACn'n_nGTC
Cuts at: 2848 4336 2848
Size: 1488 5051
XbaI T'CTAG_A
Cuts at: 3570 3570
Size: 6539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4034 4034
Size: 6539
XmaI C'CCGG_G
Cuts at: 3559 3559
Size: 6539
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PflMI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmBI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
NheI NotI NruI PacI PasI PfoI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
StuI SwaI XhoI XmnI ZraI