Restriction map for
pF1KSDA0582
(Circular) (Six-base) MAPSORT of: pF1KSDA0582.seq Check: 9392 from: 1 to: 5410
pF1KSDA0582 5410 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1127 2446 1127
Size: 1319 4091
Acc65I G'GTAC_C
Cuts at: 2424 2424
Size: 5410
AclI AA'CG_TT
Cuts at: 5250 5250
Size: 5410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 2188 3233 3665 4414 4682 185
Size: 2003 1045 432 749 268 913
Fragments arranged by size:
2003 1045 913 749 432 268
AflII C'TTAA_G
Cuts at: 1249 1249
Size: 5410
AflIII A'CryG_T
Cuts at: 2760 3866 4843 2760
Size: 1106 977 3327
Fragments arranged by size:
3327 1106 977
AgeI A'CCGG_T
Cuts at: 1919 3798 1919
Size: 1879 3531
AleI CACnn'nnGTG
Cuts at: 2384 2384
Size: 5410
AlwNI CAG_nnn'CTG
Cuts at: 528 615 2166 2211 2713 4282 528
Size: 87 1551 45 502 1569 1656
Fragments arranged by size:
1656 1569 1551 502 87 45
ApaI G_GGCC'C
Cuts at: 233 1235 233
Size: 1002 4408
ApaLI G'TGCA_C
Cuts at: 4180 4180
Size: 5410
ApoI r'AATT_y
Cuts at: 2412 4763 5019 2412
Size: 2351 256 2803
Fragments arranged by size:
2803 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5410
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5410
AvaI C'yCGr_G
Cuts at: 1057 1864 2428 1057
Size: 807 564 4039
Fragments arranged by size:
4039 807 564
BamHI G'GATC_C
Cuts at: 1624 1773 2433 1624
Size: 149 660 4601
Fragments arranged by size:
4601 660 149
BanI G'GyrC_C
Cuts at: 1508 2424 2838 3086 3121 1508
Size: 916 414 248 35 3797
Fragments arranged by size:
3797 916 414 248 35
BanII G_rGCy'C
Cuts at: 233 278 360 395 685 1235 1335 1429 1678
Size: 45 82 35 290 550 100 94 249
Cuts at: 1678 2422 3452 233
Size: 744 1030 2191
Fragments arranged by size:
2191 1030 744 550 290 249 100 94
82 45 35
BbeI G_GCGC'C
Cuts at: 1512 2842 3090 1512
Size: 1330 248 3832
Fragments arranged by size:
3832 1330 248
BbsI GAAGACnn'nnnn_
Cuts at: 173 496 2014 2105 173
Size: 323 1518 91 3478
Fragments arranged by size:
3478 1518 323 91
BbvCI CC'TCA_GC
Cuts at: 266 622 1064 266
Size: 356 442 4612
Fragments arranged by size:
4612 442 356
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1555 1589 1555
Size: 34 5376
BciVI GTATCCnnnnn_n'
Cuts at: 299 3306 4069 5410 299
Size: 3007 763 1341 299
Fragments arranged by size:
3007 1341 763 299
BclI T'GATC_A
Cuts at: 2928 2928
Size: 5410
BglII A'GATC_T
Cuts at: 1026 2923 4962 1026
Size: 1897 2039 1474
Fragments arranged by size:
2039 1897 1474
BlpI GC'TnA_GC
Cuts at: 679 698 2520 679
Size: 19 1822 3569
Fragments arranged by size:
3569 1822 19
Bme1580I G_kGCm'C
Cuts at: 233 1235 1506 1821 3033 3126 4184 233
Size: 1002 271 315 1212 93 1058 1459
Fragments arranged by size:
1459 1212 1058 1002 315 271 93
BmrI ACTGGGnnnn_n'
Cuts at: 174 858 954 1664 2221 2620 2701 3036 5182
Size: 684 96 710 557 399 81 335 2146
Cuts at: 5182 174
Size: 402
Fragments arranged by size:
2146 710 684 557 402 399 335 96
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1774 3775 1774
Size: 2001 3409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 730 829 1136 1248 1710 1769 2296 473
Size: 257 99 307 112 462 59 527 3587
Fragments arranged by size:
3587 527 462 307 257 112 99 59
Bpu10I CC'TnA_GC
Cuts at: 266 558 622 642 1064 1438 266
Size: 292 64 20 422 374 4238
Fragments arranged by size:
4238 422 374 292 64 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2092 2584 3463 3957 4255 2092
Size: 492 879 494 298 3247
Fragments arranged by size:
3247 879 494 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 449 652 801 1875 5114 449
Size: 203 149 1074 3239 745
Fragments arranged by size:
3239 1074 745 203 149
BsaAI yAC'GTr
Cuts at: 2400 2761 2400
Size: 361 5049
BsaBI GATnn'nnATC
Cuts at: 6 2156 2922 4961 6
Size: 2150 766 2039 455
Fragments arranged by size:
2150 2039 766 455
BsaHI Gr'CG_yC
Cuts at: 921 1509 2839 3087 3791 5287 921
Size: 588 1330 248 704 1496 1044
Fragments arranged by size:
1496 1330 1044 704 588 248
BsaWI w'CCGG_w
Cuts at: 1919 2597 3118 3798 4072 4219 4630 1919
Size: 678 521 680 274 147 411 2699
Fragments arranged by size:
2699 680 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 976 1006 976
Size: 30 5380
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 526 789 792 1863 1985 526
Size: 263 3 1071 122 3951
Fragments arranged by size:
3951 1071 263 122 3
BseYI C'CCAG_C
Cuts at: 1433 2833 3591 4170 4666 1433
Size: 1400 758 579 496 2177
Fragments arranged by size:
2177 1400 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 401 1503 2322 401
Size: 1102 819 3489
Fragments arranged by size:
3489 1102 819
BsiEI CG_ry'CG
Cuts at: 104 818 987 2950 2996 4206 104
Size: 714 169 1963 46 1210 1308
Fragments arranged by size:
1963 1308 1210 714 169 46
BsiHKAI G_wGCw'C
Cuts at: 2381 2422 3200 3390 4184 2381
Size: 41 778 190 794 3607
Fragments arranged by size:
3607 794 778 190 41
BsmBI CGTCTCn'nnnn_
Cuts at: 912 912
Size: 5410
Bsp1286I G_dGCh'C
Cuts at: 233 278 360 395 685 1235 1335 1429 1506
Size: 45 82 35 290 550 100 94 77
Cuts at: 1506 1678 1821 2381 2422 3033 3126 3200 3390
Size: 172 143 560 41 611 93 74 190
Cuts at: 3390 3452 4184 233
Size: 62 732 1459
Fragments arranged by size:
1459 732 611 560 550 290 190 172
143 100 94 93 82 77 74 62
45 41 35
BspEI T'CCGG_A
Cuts at: 4630 4630
Size: 5410
BspHI T'CATG_A
Cuts at: 4586 4586
Size: 5410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 318 1525 2458 2672 2974 3355 318
Size: 1207 933 214 302 381 2373
Fragments arranged by size:
2373 1207 933 381 302 214
BsrBI CCG'CTC
Cuts at: 50 210 1585 3700 3754 5240 5405 50
Size: 160 1375 2115 54 1486 165 55
Fragments arranged by size:
2115 1486 1375 165 160 55 54
BsrDI GCAATG_nn'
Cuts at: 2005 2378 3320 2005
Size: 373 942 4095
Fragments arranged by size:
4095 942 373
BsrFI r'CCGG_y
Cuts at: 813 1394 1919 2129 3406 3587 3798 813
Size: 581 525 210 1277 181 211 2425
Fragments arranged by size:
2425 1277 581 525 211 210 181
BssSI C'ACGA_G
Cuts at: 773 3679 4039 773
Size: 2906 360 2144
Fragments arranged by size:
2906 2144 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2785 2785
Size: 5410
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5410
BstEII G'GTnAC_C
Cuts at: 751 869 1641 751
Size: 118 772 4520
Fragments arranged by size:
4520 772 118
BstXI CCAn_nnnn'nTGG
Cuts at: 132 187 1768 132
Size: 55 1581 3774
Fragments arranged by size:
3774 1581 55
BstYI r'GATC_y
Cuts at: 1 1026 1624 1765 1773 2433 2900 2923 3258
Size: 1025 598 141 8 660 467 23 335
Cuts at: 3258 3504 4518 4604 4616 4962 1
Size: 246 1014 86 12 346 449
Fragments arranged by size:
1025 1014 660 598 467 449 346 335
246 141 86 23 12 8
Bsu36I CC'TnA_GG
Cuts at: 1378 1778 1378
Size: 400 5010
BtgI C'CryG_G
Cuts at: 125 443 2073 2363 125
Size: 318 1630 290 3172
Fragments arranged by size:
3172 1630 318 290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3358 3539 3702 3358
Size: 181 163 5066
Fragments arranged by size:
5066 181 163
BtsI GCAGTG_nn'
Cuts at: 1211 1261 2651 1211
Size: 50 1390 3970
Fragments arranged by size:
3970 1390 50
DraI TTT'AAA
Cuts at: 2407 2407
Size: 5410
DraIII CAC_nnn'GTG
Cuts at: 424 424
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 3114 3974 3114
Size: 860 4550
EaeI y'GGCC_r
Cuts at: 788 1924 2993 3167 3558 3585 4932 788
Size: 1136 1069 174 391 27 1347 1266
Fragments arranged by size:
1347 1266 1136 1069 391 174 27
EagI C'GGCC_G
Cuts at: 2993 2993
Size: 5410
EarI CTCTTCn'nnn_
Cuts at: 1480 1538 2024 3431 3641 1480
Size: 58 486 1407 210 3249
Fragments arranged by size:
3249 1407 486 210 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 781 3616 3928 4074 5220 781
Size: 2835 312 146 1146 971
Fragments arranged by size:
2835 1146 971 312 146
EcoICRI GAG'CTC
Cuts at: 2420 2420
Size: 5410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 185 473 730 829 1136 1248 1710 1769 2188
Size: 288 257 99 307 112 462 59 419
Cuts at: 2188 2296 3233 3665 4414 4682 185
Size: 108 937 432 749 268 913
Fragments arranged by size:
937 913 749 462 432 419 307 288
268 257 112 108 99 59
EcoNI CCTnn'n_nnAGG
Cuts at: 1467 1467
Size: 5410
EcoO109I rG'GnC_Cy
Cuts at: 229 853 1860 2547 229
Size: 624 1007 687 3092
Fragments arranged by size:
3092 1007 687 624
EcoRI G'AATT_C
Cuts at: 2412 2412
Size: 5410
EcoRV GAT'ATC
Cuts at: 1662 1662
Size: 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1048 1972 3427 1048
Size: 924 1455 3031
Fragments arranged by size:
3031 1455 924
FspI TGC'GCA
Cuts at: 3189 3189
Size: 5410
HaeII r_GCGC'y
Cuts at: 1512 2842 3090 4114 5093 1512
Size: 1330 248 1024 979 1829
Fragments arranged by size:
1829 1330 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 263 295 574 606 976 1008 1126 1158 1411
Size: 32 279 32 370 32 118 32 253
Cuts at: 1411 1443 3250 3280 3282 3312 263
Size: 32 1807 30 2 30 2361
Fragments arranged by size:
2361 1807 370 279 253 118 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2447 2447
Size: 5410
HindIII A'AGCT_T
Cuts at: 496 496
Size: 5410
KasI G'GCGC_C
Cuts at: 1508 2838 3086 1508
Size: 1330 248 3832
Fragments arranged by size:
3832 1330 248
KpnI G_GTAC'C
Cuts at: 2428 2428
Size: 5410
MluI A'CGCG_T
Cuts at: 4843 4843
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1895 2195 4081 4265 1895
Size: 300 1886 184 3040
Fragments arranged by size:
3040 1886 300 184
MscI TGG'CCA
Cuts at: 790 1926 3169 790
Size: 1136 1243 3031
Fragments arranged by size:
3031 1243 1136
MslI CAynn'nnrTG
Cuts at: 2384 3397 3524 2384
Size: 1013 127 4270
Fragments arranged by size:
4270 1013 127
MspA1I CmG'CkG
Cuts at: 710 1099 2025 2519 2833 3193 4208 4453 710
Size: 389 926 494 314 360 1015 245 1667
Fragments arranged by size:
1667 1015 926 494 389 360 314 245
NaeI GCC'GGC
Cuts at: 3589 3589
Size: 5410
NarI GG'CG_CC
Cuts at: 1509 2839 3087 1509
Size: 1330 248 3832
Fragments arranged by size:
3832 1330 248
NcoI C'CATG_G
Cuts at: 125 443 2363 125
Size: 318 1920 3172
Fragments arranged by size:
3172 1920 318
NgoMIV G'CCGG_C
Cuts at: 3587 3587
Size: 5410
NruI TCG'CGA
Cuts at: 3771 3771
Size: 5410
NspI r_CATG'y
Cuts at: 2328 2461 2961 3870 2328
Size: 133 500 909 3868
Fragments arranged by size:
3868 909 500 133
PasI CC'CwG_GG
Cuts at: 132 226 132
Size: 94 5316
PciI A'CATG_T
Cuts at: 3866 3866
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 615 1228 615
Size: 613 4797
PmeI GTTT'AAAC
Cuts at: 2407 2407
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 672 704 4549 4581 672
Size: 32 3845 32 1501
Fragments arranged by size:
3845 1501 32 32
PpuMI rG'GwC_Cy
Cuts at: 853 853
Size: 5410
PspOMI G'GGCC_C
Cuts at: 229 1231 229
Size: 1002 4408
PstI C_TGCA'G
Cuts at: 205 715 1115 2085 2123 2455 3140 205
Size: 510 400 970 38 332 685 2475
Fragments arranged by size:
2475 970 685 510 400 332 38
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5410
PvuII CAG'CTG
Cuts at: 710 1099 2025 2833 3193 710
Size: 389 926 808 360 2927
Fragments arranged by size:
2927 926 808 389 360
RsrII CG'GwC_CG
Cuts at: 3603 3603
Size: 5410
SacI G_AGCT'C
Cuts at: 2422 2422
Size: 5410
SalI G'TCGA_C
Cuts at: 2445 2445
Size: 5410
SapI GCTCTTCn'nnn_
Cuts at: 2024 3431 3641 2024
Size: 1407 210 3793
Fragments arranged by size:
3793 1407 210
SbfI CC_TGCA'GG
Cuts at: 1115 2455 1115
Size: 1340 4070
ScaI AGT'ACT
Cuts at: 943 4927 943
Size: 3984 1426
SfcI C'TryA_G
Cuts at: 33 201 711 1111 1403 2081 2119 2451 3136
Size: 168 510 400 292 678 38 332 685
Cuts at: 3136 4131 4322 33
Size: 995 191 1121
Fragments arranged by size:
1121 995 685 678 510 400 332 292
191 168 38
SfoI GGC'GCC
Cuts at: 1510 2840 3088 1510
Size: 1330 248 3832
Fragments arranged by size:
3832 1330 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5410
SmaI CCC'GGG
Cuts at: 1059 2430 1059
Size: 1371 4039
SmlI C'TyrA_G
Cuts at: 1249 2107 2563 3478 3972 4234 1249
Size: 858 456 915 494 262 2425
Fragments arranged by size:
2425 915 858 494 456 262
SnaBI TAC'GTA
Cuts at: 2400 2400
Size: 5410
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5410
SphI G_CATG'C
Cuts at: 2461 2961 2461
Size: 500 4910
SrfI GCCC'GGGC
Cuts at: 1059 1059
Size: 5410
StyI C'CwwG_G
Cuts at: 125 443 791 1637 1679 1761 2244 2363 2542
Size: 318 348 846 42 82 483 119 179
Cuts at: 2542 2896 125
Size: 354 2639
Fragments arranged by size:
2639 846 483 354 348 318 179 119
82 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 124 2396 2991 3124 3583 3794 124
Size: 2272 595 133 459 211 1740
Fragments arranged by size:
2272 1740 595 459 211 133
TatI w'GTAC_w
Cuts at: 941 4925 941
Size: 3984 1426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1441 1473 4927 4959 1441
Size: 32 3454 32 1892
Fragments arranged by size:
3454 1892 32 32
Tth111I GACn'n_nGTC
Cuts at: 1105 3205 1105
Size: 2100 3310
XbaI T'CTAG_A
Cuts at: 352 2439 352
Size: 2087 3323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1282 1635 1744 2370 2903 1282
Size: 353 109 626 533 3789
Fragments arranged by size:
3789 626 533 353 109
XmaI C'CCGG_G
Cuts at: 1057 2428 1057
Size: 1371 4039
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstZ17I ClaI CspCI FseI FspAI HpaI MfeI
NdeI NheI NotI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SspI StuI SwaI XhoI XmnI ZraI