Restriction map for
pF1KSDA0576
(Circular) (Six-base) MAPSORT of: pF1KSDA0576.seq Check: 7616 from: 1 to: 6320
pF1KSDA0576 6320 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2512 2512
Size: 6320
AccI GT'mk_AC
Cuts at: 3358 3358
Size: 6320
Acc65I G'GTAC_C
Cuts at: 3336 3336
Size: 6320
AclI AA'CG_TT
Cuts at: 6160 6160
Size: 6320
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 4145 4577 5324 5592 735
Size: 3410 432 747 268 1463
Fragments arranged by size:
3410 1463 747 432 268
AflIII A'CryG_T
Cuts at: 608 1770 3672 4776 5753 608
Size: 1162 1902 1104 977 1175
Fragments arranged by size:
1902 1175 1162 1104 977
AgeI A'CCGG_T
Cuts at: 4708 4708
Size: 6320
AhdI GACnn_n'nnGTC
Cuts at: 138 1285 138
Size: 1147 5173
AleI CACnn'nnGTG
Cuts at: 613 1443 613
Size: 830 5490
AlwNI CAG_nnn'CTG
Cuts at: 3625 5192 3625
Size: 1567 4753
ApaLI G'TGCA_C
Cuts at: 2449 2534 5090 2449
Size: 85 2556 3679
Fragments arranged by size:
3679 2556 85
ApoI r'AATT_y
Cuts at: 481 784 1195 1272 1393 1473 1780 2415 3041
Size: 303 411 77 121 80 307 635 626
Cuts at: 3041 3247 3324 5673 5929 481
Size: 206 77 2349 256 872
Fragments arranged by size:
2349 872 635 626 411 307 303 256
206 121 80 77 77
AseI AT'TA_AT
Cuts at: 20 569 20
Size: 549 5771
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6320
AvaI C'yCGr_G
Cuts at: 135 3340 135
Size: 3205 3115
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2022 2055 2022
Size: 33 6287
BamHI G'GATC_C
Cuts at: 1353 3345 1353
Size: 1992 4328
BanI G'GyrC_C
Cuts at: 674 2500 3220 3336 3750 3998 4033 674
Size: 1826 720 116 414 248 35 2961
Fragments arranged by size:
2961 1826 720 414 248 116 35
BanII G_rGCy'C
Cuts at: 1115 3334 4364 1115
Size: 2219 1030 3071
Fragments arranged by size:
3071 2219 1030
BbeI G_GCGC'C
Cuts at: 3754 4002 3754
Size: 248 6072
BbsI GAAGACnn'nnnn_
Cuts at: 215 3109 215
Size: 2894 3426
BciVI GTATCCnnnnn_n'
Cuts at: 4218 4979 6320 4218
Size: 761 1341 4218
Fragments arranged by size:
4218 1341 761
BclI T'GATC_A
Cuts at: 1905 3840 1905
Size: 1935 4385
BfrBI ATG'CAT
Cuts at: 3006 3006
Size: 6320
BglII A'GATC_T
Cuts at: 3835 5872 3835
Size: 2037 4283
BlpI GC'TnA_GC
Cuts at: 3432 3432
Size: 6320
Bme1580I G_kGCm'C
Cuts at: 677 1698 2453 2538 3945 4038 5094 677
Size: 1021 755 85 1407 93 1056 1903
Fragments arranged by size:
1903 1407 1056 1021 755 93 85
BmrI ACTGGGnnnn_n'
Cuts at: 985 2573 3532 3613 3948 6092 985
Size: 1588 959 81 335 2144 1213
Fragments arranged by size:
2144 1588 1213 959 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3134 4687 3134
Size: 1553 4767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 1462 3100 149
Size: 1313 1638 3369
Fragments arranged by size:
3369 1638 1313
Bpu10I CC'TnA_GC
Cuts at: 2716 2716
Size: 6320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3319 3496 4375 4867 5165 3319
Size: 177 879 492 298 4474
Fragments arranged by size:
4474 879 492 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 125 2263 6024 125
Size: 2138 3761 421
Fragments arranged by size:
3761 2138 421
BsaAI yAC'GTr
Cuts at: 611 3312 3673 611
Size: 2701 361 3258
Fragments arranged by size:
3258 2701 361
BsaBI GATnn'nnATC
Cuts at: 6 1226 3834 5871 6
Size: 1220 2608 2037 455
Fragments arranged by size:
2608 2037 1220 455
BsaHI Gr'CG_yC
Cuts at: 3751 3999 4701 6197 3751
Size: 248 702 1496 3874
Fragments arranged by size:
3874 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3509 4030 4708 4982 5129 5540 3509
Size: 521 678 274 147 411 4289
Fragments arranged by size:
4289 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2467 2497 2467
Size: 30 6290
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 614 937 2200 2485 2488 614
Size: 323 1263 285 3 4446
Fragments arranged by size:
4446 1263 323 285 3
BseYI C'CCAG_C
Cuts at: 3745 4503 5080 5576 3745
Size: 758 577 496 4489
Fragments arranged by size:
4489 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2490 2490
Size: 6320
BsiEI CG_ry'CG
Cuts at: 104 3862 3908 5116 104
Size: 3758 46 1208 1308
Fragments arranged by size:
3758 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1115 2043 2192 2453 2538 3334 4112 4302 5094
Size: 928 149 261 85 796 778 190 792
Cuts at: 5094 1115
Size: 2341
Fragments arranged by size:
2341 928 796 792 778 261 190 149
85
BsmI GAATG_Cn'
Cuts at: 430 1555 430
Size: 1125 5195
Bsp1286I G_dGCh'C
Cuts at: 677 1115 1698 2043 2192 2453 2538 3334 3945
Size: 438 583 345 149 261 85 796 611
Cuts at: 3945 4038 4112 4302 4364 5094 677
Size: 93 74 190 62 730 1903
Fragments arranged by size:
1903 796 730 611 583 438 345 261
190 149 93 85 74 62
BspEI T'CCGG_A
Cuts at: 5540 5540
Size: 6320
BspHI T'CATG_A
Cuts at: 2520 5496 2520
Size: 2976 3344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2512 2777 3370 3584 3886 4267 2512
Size: 265 593 214 302 381 4565
Fragments arranged by size:
4565 593 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 2904 4612 4666 6150 6315 50
Size: 2854 1708 54 1484 165 55
Fragments arranged by size:
2854 1708 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1448 1951 2085 2559 4232 1448
Size: 503 134 474 1673 3536
Fragments arranged by size:
3536 1673 503 474 134
BsrFI r'CCGG_y
Cuts at: 4318 4499 4708 4318
Size: 181 209 5930
Fragments arranged by size:
5930 209 181
BsrGI T'GTAC_A
Cuts at: 2615 2615
Size: 6320
BssSI C'ACGA_G
Cuts at: 4591 4949 4591
Size: 358 5962
BstAPI GCAn_nnn'nTGC
Cuts at: 3697 3697
Size: 6320
BstBI TT'CG_AA
Cuts at: 110 4681 110
Size: 4571 1749
BstYI r'GATC_y
Cuts at: 1 1353 2596 3345 3812 3835 4170 4416 5428
Size: 1352 1243 749 467 23 335 246 1012
Cuts at: 5428 5514 5526 5872 1
Size: 86 12 346 449
Fragments arranged by size:
1352 1243 1012 749 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 176 176
Size: 6320
BtgI C'CryG_G
Cuts at: 125 125
Size: 6320
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4270 4451 4614 4270
Size: 181 163 5976
Fragments arranged by size:
5976 181 163
BtsI GCAGTG_nn'
Cuts at: 282 1093 1115 1995 2043 3563 282
Size: 811 22 880 48 1520 3039
Fragments arranged by size:
3039 1520 880 811 48 22
DraI TTT'AAA
Cuts at: 1478 1703 3319 1478
Size: 225 1616 4479
Fragments arranged by size:
4479 1616 225
DrdI GACnn_nn'nnGTC
Cuts at: 4026 4884 4026
Size: 858 5462
EaeI y'GGCC_r
Cuts at: 3012 3905 4079 4470 4497 5842 3012
Size: 893 174 391 27 1345 3490
Fragments arranged by size:
3490 1345 893 391 174 27
EagI C'GGCC_G
Cuts at: 3905 3905
Size: 6320
EarI CTCTTCn'nnn_
Cuts at: 281 410 591 1165 4343 4553 281
Size: 129 181 574 3178 210 2048
Fragments arranged by size:
3178 2048 574 210 181 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4528 4838 4984 6130 4528
Size: 310 146 1146 4718
Fragments arranged by size:
4718 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1113 3332 1113
Size: 2219 4101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 735 1462 3100 4145 4577 5324 5592 149
Size: 586 727 1638 1045 432 747 268 877
Fragments arranged by size:
1638 1045 877 747 727 586 432 268
EcoO109I rG'GnC_Cy
Cuts at: 3459 3459
Size: 6320
EcoRI G'AATT_C
Cuts at: 1272 3324 1272
Size: 2052 4268
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4339 4339
Size: 6320
FspI TGC'GCA
Cuts at: 4101 4101
Size: 6320
HaeII r_GCGC'y
Cuts at: 3754 4002 5024 6003 3754
Size: 248 1022 979 4071
Fragments arranged by size:
4071 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 1271 1303 2797 2829 4162 4192 4194
Size: 32 1115 32 1494 32 1333 30 2
Cuts at: 4194 4224 124
Size: 30 2220
Fragments arranged by size:
2220 1494 1333 1115 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 191 1745 2285 2322 3359 191
Size: 1554 540 37 1037 3152
Fragments arranged by size:
3152 1554 1037 540 37
HpaI GTT'AAC
Cuts at: 1745 2285 1745
Size: 540 5780
KasI G'GCGC_C
Cuts at: 3750 3998 3750
Size: 248 6072
KpnI G_GTAC'C
Cuts at: 3340 3340
Size: 6320
MfeI C'AATT_G
Cuts at: 2889 2889
Size: 6320
MluI A'CGCG_T
Cuts at: 5753 5753
Size: 6320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 122 748 1361 1705 4991 5175 122
Size: 626 613 344 3286 184 1267
Fragments arranged by size:
3286 1267 626 613 344 184
MscI TGG'CCA
Cuts at: 4081 4081
Size: 6320
MslI CAynn'nnrTG
Cuts at: 461 613 1443 1496 2105 3001 4309 4436 461
Size: 152 830 53 609 896 1308 127 2345
Fragments arranged by size:
2345 1308 896 830 609 152 127 53
MspA1I CmG'CkG
Cuts at: 169 2036 3431 3745 4105 5118 5363 169
Size: 1867 1395 314 360 1013 245 1126
Fragments arranged by size:
1867 1395 1126 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4501 4501
Size: 6320
NarI GG'CG_CC
Cuts at: 3751 3999 3751
Size: 248 6072
NcoI C'CATG_G
Cuts at: 125 125
Size: 6320
NdeI CA'TA_TG
Cuts at: 938 2998 938
Size: 2060 4260
NgoMIV G'CCGG_C
Cuts at: 4499 4499
Size: 6320
NsiI A_TGCA'T
Cuts at: 3008 3008
Size: 6320
NspI r_CATG'y
Cuts at: 460 890 1594 1733 1774 2004 3010 3373 3873
Size: 430 704 139 41 230 1006 363 500
Cuts at: 3873 4780 460
Size: 907 2000
Fragments arranged by size:
2000 1006 907 704 500 430 363 230
139 41
PciI A'CATG_T
Cuts at: 1770 4776 1770
Size: 3006 3314
PmeI GTTT'AAAC
Cuts at: 3319 3319
Size: 6320
PmlI CAC'GTG
Cuts at: 611 611
Size: 6320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2766 2798 5459 5491 2766
Size: 32 2661 32 3595
Fragments arranged by size:
3595 2661 32 32
PsiI TTA'TAA
Cuts at: 779 1619 2921 779
Size: 840 1302 4178
Fragments arranged by size:
4178 1302 840
PstI C_TGCA'G
Cuts at: 2050 2392 3367 4052 2050
Size: 342 975 685 4318
Fragments arranged by size:
4318 975 685 342
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6320
PvuII CAG'CTG
Cuts at: 169 2036 3745 4105 169
Size: 1867 1709 360 2384
Fragments arranged by size:
2384 1867 1709 360
RsrII CG'GwC_CG
Cuts at: 4515 4515
Size: 6320
SacI G_AGCT'C
Cuts at: 1115 3334 1115
Size: 2219 4101
SalI G'TCGA_C
Cuts at: 3357 3357
Size: 6320
SapI GCTCTTCn'nnn_
Cuts at: 281 1165 4343 4553 281
Size: 884 3178 210 2048
Fragments arranged by size:
3178 2048 884 210
SbfI CC_TGCA'GG
Cuts at: 3367 3367
Size: 6320
ScaI AGT'ACT
Cuts at: 5837 5837
Size: 6320
SexAI A'CCwGG_T
Cuts at: 1537 1537
Size: 6320
SfcI C'TryA_G
Cuts at: 33 1154 1160 2046 2388 2964 3363 4048 5041
Size: 1121 6 886 342 576 399 685 993
Cuts at: 5041 5232 33
Size: 191 1121
Fragments arranged by size:
1121 1121 993 886 685 576 399 342
191 6
SfoI GGC'GCC
Cuts at: 3752 4000 3752
Size: 248 6072
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6320
SmaI CCC'GGG
Cuts at: 137 3342 137
Size: 3205 3115
SmlI C'TyrA_G
Cuts at: 3298 3475 4390 4882 5144 3298
Size: 177 915 492 262 4474
Fragments arranged by size:
4474 915 492 262 177
SnaBI TAC'GTA
Cuts at: 3312 3312
Size: 6320
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6320
SphI G_CATG'C
Cuts at: 460 3010 3373 3873 460
Size: 2550 363 500 2907
Fragments arranged by size:
2907 2550 500 363
SspI AAT'ATT
Cuts at: 318 318
Size: 6320
StyI C'CwwG_G
Cuts at: 125 2850 3454 3808 125
Size: 2725 604 354 2637
Fragments arranged by size:
2725 2637 604 354
SwaI ATTT'AAAT
Cuts at: 1703 1703
Size: 6320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 603 967 1270 3208 3903 4036 4495 4704 603
Size: 364 303 1938 695 133 459 209 2219
Fragments arranged by size:
2219 1938 695 459 364 303 209 133
TatI w'GTAC_w
Cuts at: 2615 5835 2615
Size: 3220 3100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5837 5869 5837
Size: 32 6288
Tth111I GACn'n_nGTC
Cuts at: 4117 4117
Size: 6320
XbaI T'CTAG_A
Cuts at: 3018 3351 3018
Size: 333 5987
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 3264 3815 2287
Size: 977 551 4792
Fragments arranged by size:
4792 977 551
XmaI C'CCGG_G
Cuts at: 135 3340 135
Size: 3205 3115
XmnI GAAnn'nnTTC
Cuts at: 485 700 955 2168 485
Size: 215 255 1213 4637
Fragments arranged by size:
4637 1213 255 215
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PmeI PmlI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BbvCI BcgI BglI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII NheI NotI NruI PacI PasI
PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SrfI StuI XhoI ZraI