Restriction map for
pF1KSDA0554
(Circular) (Six-base) MAPSORT of: pF1KSDA0554.seq Check: 2579 from: 1 to: 4990
pF1KSDA0554 4990 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 834 834
Size: 4990
AccI GT'mk_AC
Cuts at: 325 1448 2026 325
Size: 1123 578 3289
Fragments arranged by size:
3289 1123 578
Acc65I G'GTAC_C
Cuts at: 2004 2004
Size: 4990
AclI AA'CG_TT
Cuts at: 4830 4830
Size: 4990
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1260 2813 3245 3994 4262 1260
Size: 1553 432 749 268 1988
Fragments arranged by size:
1988 1553 749 432 268
AflIII A'CryG_T
Cuts at: 2340 3446 4423 2340
Size: 1106 977 2907
Fragments arranged by size:
2907 1106 977
AgeI A'CCGG_T
Cuts at: 3378 3378
Size: 4990
AlwNI CAG_nnn'CTG
Cuts at: 450 2293 3862 450
Size: 1843 1569 1578
Fragments arranged by size:
1843 1578 1569
ApaLI G'TGCA_C
Cuts at: 3760 3760
Size: 4990
ApoI r'AATT_y
Cuts at: 712 973 1029 1060 1397 1546 1992 4343 4599
Size: 261 56 31 337 149 446 2351 256
Cuts at: 4599 712
Size: 1103
Fragments arranged by size:
2351 1103 446 337 261 256 149 56
31
AscI GG'CGCG_CC
Cuts at: 1603 1603
Size: 4990
AseI AT'TA_AT
Cuts at: 20 20
Size: 4990
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4990
AvaI C'yCGr_G
Cuts at: 2008 2008
Size: 4990
BamHI G'GATC_C
Cuts at: 2013 2013
Size: 4990
BanI G'GyrC_C
Cuts at: 136 2004 2418 2666 2701 136
Size: 1868 414 248 35 2425
Fragments arranged by size:
2425 1868 414 248 35
BanII G_rGCy'C
Cuts at: 146 1678 1769 2002 3032 146
Size: 1532 91 233 1030 2104
Fragments arranged by size:
2104 1532 1030 233 91
BbeI G_GCGC'C
Cuts at: 2422 2670 2422
Size: 248 4742
BbsI GAAGACnn'nnnn_
Cuts at: 826 1877 826
Size: 1051 3939
BbvCI CC'TCA_GC
Cuts at: 1565 1565
Size: 4990
BciVI GTATCCnnnnn_n'
Cuts at: 2886 3649 4990 2886
Size: 763 1341 2886
Fragments arranged by size:
2886 1341 763
BclI T'GATC_A
Cuts at: 903 2508 903
Size: 1605 3385
BglI GCCn_nnn'nGGC
Cuts at: 1561 1682 1561
Size: 121 4869
BglII A'GATC_T
Cuts at: 2503 4542 2503
Size: 2039 2951
BlpI GC'TnA_GC
Cuts at: 2100 2100
Size: 4990
Bme1580I G_kGCm'C
Cuts at: 139 2613 2706 3764 139
Size: 2474 93 1058 1365
Fragments arranged by size:
2474 1365 1058 93
BmgBI CAC'GTC
Cuts at: 1787 1787
Size: 4990
BmrI ACTGGGnnnn_n'
Cuts at: 1191 2200 2281 2616 4762 1191
Size: 1009 81 335 2146 1419
Fragments arranged by size:
2146 1419 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 4990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 983 691
Size: 292 4698
Bpu10I CC'TnA_GC
Cuts at: 1565 1565
Size: 4990
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 1288 2164 3043 3537 3835 220
Size: 1068 876 879 494 298 1375
Fragments arranged by size:
1375 1068 879 876 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1466 1531 4694 1466
Size: 65 3163 1762
Fragments arranged by size:
3163 1762 65
BsaAI yAC'GTr
Cuts at: 1980 2341 1980
Size: 361 4629
BsaBI GATnn'nnATC
Cuts at: 6 853 2502 4541 6
Size: 847 1649 2039 455
Fragments arranged by size:
2039 1649 847 455
BsaHI Gr'CG_yC
Cuts at: 2419 2667 3371 4867 2419
Size: 248 704 1496 2542
Fragments arranged by size:
2542 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1320 2177 2698 3378 3652 3799 4210 1320
Size: 857 521 680 274 147 411 2100
Fragments arranged by size:
2100 857 680 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1052 1711 1777 1052
Size: 659 66 4265
Fragments arranged by size:
4265 659 66
BseYI C'CCAG_C
Cuts at: 131 1308 1476 1584 2413 3171 3750 4246 131
Size: 1177 168 108 829 758 579 496 875
Fragments arranged by size:
1177 875 829 758 579 496 168 108
BsiEI CG_ry'CG
Cuts at: 104 2530 2576 3786 104
Size: 2426 46 1210 1308
Fragments arranged by size:
2426 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 146 2002 2780 2970 3764 146
Size: 1856 778 190 794 1372
Fragments arranged by size:
1856 1372 794 778 190
BsmI GAATG_Cn'
Cuts at: 1895 1895
Size: 4990
Bsp1286I G_dGCh'C
Cuts at: 139 146 1678 1769 2002 2613 2706 2780 2970
Size: 7 1532 91 233 611 93 74 190
Cuts at: 2970 3032 3764 139
Size: 62 732 1365
Fragments arranged by size:
1532 1365 732 611 233 190 93 91
74 62 7
BspEI T'CCGG_A
Cuts at: 4210 4210
Size: 4990
BspHI T'CATG_A
Cuts at: 1247 4166 1247
Size: 2919 2071
BspMI ACCTGCnnnn'nnnn_
Cuts at: 834 2038 2252 2554 2935 834
Size: 1204 214 302 381 2889
Fragments arranged by size:
2889 1204 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1616 3280 3334 4820 4985 50
Size: 1566 1664 54 1486 165 55
Fragments arranged by size:
1664 1566 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2900 2900
Size: 4990
BsrFI r'CCGG_y
Cuts at: 1577 1607 2986 3167 3378 1577
Size: 30 1379 181 211 3189
Fragments arranged by size:
3189 1379 211 181 30
BssHII G'CGCG_C
Cuts at: 1603 1603
Size: 4990
BssSI C'ACGA_G
Cuts at: 3259 3619 3259
Size: 360 4630
BstAPI GCAn_nnn'nTGC
Cuts at: 2365 2365
Size: 4990
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4990
BstYI r'GATC_y
Cuts at: 1 2013 2480 2503 2838 3084 4098 4184 4196
Size: 2012 467 23 335 246 1014 86 12
Cuts at: 4196 4542 1
Size: 346 449
Fragments arranged by size:
2012 1014 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 326 326
Size: 4990
BtgI C'CryG_G
Cuts at: 1733 1733
Size: 4990
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1895 2938 3119 3282 1895
Size: 1043 181 163 3603
Fragments arranged by size:
3603 1043 181 163
BtsI GCAGTG_nn'
Cuts at: 1269 2231 1269
Size: 962 4028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1047 1082 1722 1757 1047
Size: 35 640 35 4280
Fragments arranged by size:
4280 640 35 35
DraI TTT'AAA
Cuts at: 1987 1987
Size: 4990
DraIII CAC_nnn'GTG
Cuts at: 182 182
Size: 4990
DrdI GACnn_nn'nnGTC
Cuts at: 613 2694 3554 613
Size: 2081 860 2049
Fragments arranged by size:
2081 2049 860
EaeI y'GGCC_r
Cuts at: 483 1090 1730 2573 2747 3138 3165 4512 483
Size: 607 640 843 174 391 27 1347 961
Fragments arranged by size:
1347 961 843 640 607 391 174 27
EagI C'GGCC_G
Cuts at: 2573 2573
Size: 4990
EarI CTCTTCn'nnn_
Cuts at: 1910 3011 3221 1910
Size: 1101 210 3679
Fragments arranged by size:
3679 1101 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 583 3196 3508 3654 4800 583
Size: 2613 312 146 1146 773
Fragments arranged by size:
2613 1146 773 312 146
EcoICRI GAG'CTC
Cuts at: 144 2000 144
Size: 1856 3134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 983 1260 2813 3245 3994 4262 691
Size: 292 277 1553 432 749 268 1419
Fragments arranged by size:
1553 1419 749 432 292 277 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1778 1778
Size: 4990
EcoO109I rG'GnC_Cy
Cuts at: 2127 2127
Size: 4990
EcoRI G'AATT_C
Cuts at: 1992 1992
Size: 4990
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3007 3007
Size: 4990
FspI TGC'GCA
Cuts at: 2769 2769
Size: 4990
HaeII r_GCGC'y
Cuts at: 2422 2670 3694 4673 2422
Size: 248 1024 979 2739
Fragments arranged by size:
2739 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 451 483 682 714 2830 2860 2862 2892 451
Size: 32 199 32 2116 30 2 30 2549
Fragments arranged by size:
2549 2116 199 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1650 2027 1650
Size: 377 4613
HindIII A'AGCT_T
Cuts at: 338 935 1111 338
Size: 597 176 4217
Fragments arranged by size:
4217 597 176
KasI G'GCGC_C
Cuts at: 2418 2666 2418
Size: 248 4742
KpnI G_GTAC'C
Cuts at: 2008 2008
Size: 4990
MfeI C'AATT_G
Cuts at: 899 899
Size: 4990
MluI A'CGCG_T
Cuts at: 4423 4423
Size: 4990
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 374 3661 3845 374
Size: 3287 184 1519
Fragments arranged by size:
3287 1519 184
MscI TGG'CCA
Cuts at: 1732 2749 1732
Size: 1017 3973
MslI CAynn'nnrTG
Cuts at: 2977 3104 2977
Size: 127 4863
MspA1I CmG'CkG
Cuts at: 924 2099 2413 2773 3788 4033 924
Size: 1175 314 360 1015 245 1881
Fragments arranged by size:
1881 1175 1015 360 314 245
NaeI GCC'GGC
Cuts at: 1579 1609 3169 1579
Size: 30 1560 3400
Fragments arranged by size:
3400 1560 30
NarI GG'CG_CC
Cuts at: 2419 2667 2419
Size: 248 4742
NdeI CA'TA_TG
Cuts at: 825 1938 825
Size: 1113 3877
NgoMIV G'CCGG_C
Cuts at: 1577 1607 3167 1577
Size: 30 1560 3400
Fragments arranged by size:
3400 1560 30
NruI TCG'CGA
Cuts at: 3351 3351
Size: 4990
NspI r_CATG'y
Cuts at: 2041 2541 3450 2041
Size: 500 909 3581
Fragments arranged by size:
3581 909 500
PciI A'CATG_T
Cuts at: 3446 3446
Size: 4990
PflMI CCAn_nnn'nTGG
Cuts at: 131 859 131
Size: 728 4262
PfoI T'CCnGG_A
Cuts at: 960 960
Size: 4990
PmeI GTTT'AAAC
Cuts at: 1987 1987
Size: 4990
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4129 4161 4129
Size: 32 4958
PsiI TTA'TAA
Cuts at: 941 941
Size: 4990
PstI C_TGCA'G
Cuts at: 1370 2035 2720 1370
Size: 665 685 3640
Fragments arranged by size:
3640 685 665
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4990
PvuII CAG'CTG
Cuts at: 924 2413 2773 924
Size: 1489 360 3141
Fragments arranged by size:
3141 1489 360
RsrII CG'GwC_CG
Cuts at: 3183 3183
Size: 4990
SacI G_AGCT'C
Cuts at: 146 2002 146
Size: 1856 3134
SalI G'TCGA_C
Cuts at: 2025 2025
Size: 4990
SapI GCTCTTCn'nnn_
Cuts at: 3011 3221 3011
Size: 210 4780
SbfI CC_TGCA'GG
Cuts at: 2035 2035
Size: 4990
ScaI AGT'ACT
Cuts at: 586 4507 586
Size: 3921 1069
SfcI C'TryA_G
Cuts at: 33 1366 2031 2716 3711 3902 33
Size: 1333 665 685 995 191 1121
Fragments arranged by size:
1333 1121 995 685 665 191
SfoI GGC'GCC
Cuts at: 2420 2668 2420
Size: 248 4742
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4990
SmaI CCC'GGG
Cuts at: 2010 2010
Size: 4990
SmlI C'TyrA_G
Cuts at: 199 1303 2143 3058 3552 3814 199
Size: 1104 840 915 494 262 1375
Fragments arranged by size:
1375 1104 915 840 494 262
SnaBI TAC'GTA
Cuts at: 1980 1980
Size: 4990
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4990
SphI G_CATG'C
Cuts at: 2041 2541 2041
Size: 500 4490
SspI AAT'ATT
Cuts at: 736 736
Size: 4990
StuI AGG'CCT
Cuts at: 1555 1555
Size: 4990
StyI C'CwwG_G
Cuts at: 2122 2476 2122
Size: 354 4636
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1300 1656 2571 2704 3163 3374 1300
Size: 356 915 133 459 211 2916
Fragments arranged by size:
2916 915 459 356 211 133
TatI w'GTAC_w
Cuts at: 584 4505 584
Size: 3921 1069
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4507 4539 4507
Size: 32 4958
Tth111I GACn'n_nGTC
Cuts at: 2785 2785
Size: 4990
XbaI T'CTAG_A
Cuts at: 2019 2019
Size: 4990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2483
Size: 4990
XmaI C'CCGG_G
Cuts at: 2008 2008
Size: 4990
XmnI GAAnn'nnTTC
Cuts at: 1830 1830
Size: 4990
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AvrII BaeI BcgI BfrBI BmtI BsaXI BsgI BsiWI
BsmBI BsrGI BstEII BstXI Bsu36I ClaI EcoRV FseI
FspAI HpaI NcoI NheI NotI NsiI PacI PasI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI SwaI XhoI ZraI