Restriction map for
pF1KSDA0552
(Circular) (Six-base) MAPSORT of: pF1KSDA0552.seq Check: 613 from: 1 to: 5158
pF1KSDA0552 5158 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1245 1290 1738 1245
Size: 45 448 4665
Fragments arranged by size:
4665 448 45
AccI GT'mk_AC
Cuts at: 2194 2194
Size: 5158
Acc65I G'GTAC_C
Cuts at: 292 2172 292
Size: 1880 3278
AclI AA'CG_TT
Cuts at: 4998 4998
Size: 5158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 1513 1602 2981 3413 4162 4430 1501
Size: 12 89 1379 432 749 268 2229
Fragments arranged by size:
2229 1379 749 432 268 89 12
AflIII A'CryG_T
Cuts at: 2508 3614 4591 2508
Size: 1106 977 3075
Fragments arranged by size:
3075 1106 977
AgeI A'CCGG_T
Cuts at: 3546 3546
Size: 5158
AlwNI CAG_nnn'CTG
Cuts at: 837 942 1095 1182 1491 2022 2461 4030 837
Size: 105 153 87 309 531 439 1569 1965
Fragments arranged by size:
1965 1569 531 439 309 153 105 87
ApaI G_GGCC'C
Cuts at: 703 1842 1896 703
Size: 1139 54 3965
Fragments arranged by size:
3965 1139 54
ApaLI G'TGCA_C
Cuts at: 1079 3928 1079
Size: 2849 2309
ApoI r'AATT_y
Cuts at: 636 654 2160 4511 4767 636
Size: 18 1506 2351 256 1027
Fragments arranged by size:
2351 1506 1027 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 5158
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5158
AvaI C'yCGr_G
Cuts at: 338 1895 2119 2176 338
Size: 1557 224 57 3320
Fragments arranged by size:
3320 1557 224 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 511 544 511
Size: 33 5125
BamHI G'GATC_C
Cuts at: 2181 2181
Size: 5158
BanI G'GyrC_C
Cuts at: 292 840 1909 2172 2586 2834 2869 292
Size: 548 1069 263 414 248 35 2581
Fragments arranged by size:
2581 1069 548 414 263 248 35
BanII G_rGCy'C
Cuts at: 539 703 1021 1842 1896 2170 3200 539
Size: 164 318 821 54 274 1030 2497
Fragments arranged by size:
2497 1030 821 318 274 164 54
BbeI G_GCGC'C
Cuts at: 2590 2838 2590
Size: 248 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1645 1679 1645
Size: 34 5124
BciVI GTATCCnnnnn_n'
Cuts at: 3054 3817 5158 3054
Size: 763 1341 3054
Fragments arranged by size:
3054 1341 763
BclI T'GATC_A
Cuts at: 2676 2676
Size: 5158
BglI GCCn_nnn'nGGC
Cuts at: 341 707 716 1016 1771 1876 341
Size: 366 9 300 755 105 3623
Fragments arranged by size:
3623 755 366 300 105 9
BglII A'GATC_T
Cuts at: 1469 2671 4710 1469
Size: 1202 2039 1917
Fragments arranged by size:
2039 1917 1202
BlpI GC'TnA_GC
Cuts at: 1600 1883 1987 2268 1600
Size: 283 104 281 4490
Fragments arranged by size:
4490 283 281 104
Bme1580I G_kGCm'C
Cuts at: 664 703 1083 1272 1842 1896 2781 2874 3932
Size: 39 380 189 570 54 885 93 1058
Cuts at: 3932 664
Size: 1890
Fragments arranged by size:
1890 1058 885 570 380 189 93 54
39
BmgBI CAC'GTC
Cuts at: 1267 1267
Size: 5158
BmrI ACTGGGnnnn_n'
Cuts at: 1070 2368 2449 2784 4930 1070
Size: 1298 81 335 2146 1298
Fragments arranged by size:
2146 1298 1298 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3523 3523
Size: 5158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 731 804 954 1117 1171 1204 1396 1660
Size: 574 73 150 163 54 33 192 264
Cuts at: 1660 1963 2044 157
Size: 303 81 3271
Fragments arranged by size:
3271 574 303 264 192 163 150 81
73 54 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1660 2332 3211 3705 4003 1660
Size: 672 879 494 298 2815
Fragments arranged by size:
2815 879 672 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 406 4862 406
Size: 4456 702
BsaAI yAC'GTr
Cuts at: 1995 2148 2509 1995
Size: 153 361 4644
Fragments arranged by size:
4644 361 153
BsaBI GATnn'nnATC
Cuts at: 6 2670 4709 6
Size: 2664 2039 455
Fragments arranged by size:
2664 2039 455
BsaHI Gr'CG_yC
Cuts at: 2587 2835 3539 5035 2587
Size: 248 704 1496 2710
Fragments arranged by size:
2710 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2345 2866 3546 3820 3967 4378 2345
Size: 521 680 274 147 411 3125
Fragments arranged by size:
3125 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 539 569 729 750 759 780 1093 1123 2058
Size: 30 160 21 9 21 313 30 935
Cuts at: 2058 2088 539
Size: 30 3609
Fragments arranged by size:
3609 935 313 160 30 30 30 21
21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1114 1201 1354 1603 1939 1960 2104 1114
Size: 87 153 249 336 21 144 4168
Fragments arranged by size:
4168 336 249 153 144 87 21
BseYI C'CCAG_C
Cuts at: 538 1271 1280 1644 2078 2581 3339 3918 4414
Size: 733 9 364 434 503 758 579 496
Cuts at: 4414 538
Size: 1282
Fragments arranged by size:
1282 758 733 579 503 496 434 364
9
BsiEI CG_ry'CG
Cuts at: 104 607 1706 2698 2744 3954 104
Size: 503 1099 992 46 1210 1308
Fragments arranged by size:
1308 1210 1099 992 503 46
BsiHKAI G_wGCw'C
Cuts at: 589 1083 2170 2948 3138 3932 589
Size: 494 1087 778 190 794 1815
Fragments arranged by size:
1815 1087 794 778 494 190
BsmBI CGTCTCn'nnnn_
Cuts at: 133 133
Size: 5158
Bsp1286I G_dGCh'C
Cuts at: 539 589 664 703 1021 1083 1272 1842 1896
Size: 50 75 39 318 62 189 570 54
Cuts at: 1896 2170 2781 2874 2948 3138 3200 3932 539
Size: 274 611 93 74 190 62 732 1765
Fragments arranged by size:
1765 732 611 570 318 274 190 189
93 75 74 62 62 54 50 39
BspEI T'CCGG_A
Cuts at: 4378 4378
Size: 5158
BspHI T'CATG_A
Cuts at: 4334 4334
Size: 5158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1245 1290 1738 2206 2420 2722 3103 1245
Size: 45 448 468 214 302 381 3300
Fragments arranged by size:
3300 468 448 381 302 214 45
BsrBI CCG'CTC
Cuts at: 50 1104 1191 1209 1829 1887 3448 3502 4988
Size: 1054 87 18 620 58 1561 54 1486
Cuts at: 4988 5153 50
Size: 165 55
Fragments arranged by size:
1561 1486 1054 620 165 87 58 55
54 18
BsrDI GCAATG_nn'
Cuts at: 3068 3068
Size: 5158
BsrFI r'CCGG_y
Cuts at: 1680 3154 3335 3546 1680
Size: 1474 181 211 3292
Fragments arranged by size:
3292 1474 211 181
BssHII G'CGCG_C
Cuts at: 153 1275 153
Size: 1122 4036
BssSI C'ACGA_G
Cuts at: 3427 3787 3427
Size: 360 4798
BstAPI GCAn_nnn'nTGC
Cuts at: 1305 2059 2533 1305
Size: 754 474 3930
Fragments arranged by size:
3930 754 474
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5158
BstXI CCAn_nnnn'nTGG
Cuts at: 558 1462 558
Size: 904 4254
BstYI r'GATC_y
Cuts at: 1 1469 2181 2648 2671 3006 3252 4266 4352
Size: 1468 712 467 23 335 246 1014 86
Cuts at: 4352 4364 4710 1
Size: 12 346 449
Fragments arranged by size:
1468 1014 712 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 686 686
Size: 5158
BtgI C'CryG_G
Cuts at: 125 193 233 1694 1796 125
Size: 68 40 1461 102 3487
Fragments arranged by size:
3487 1461 102 68 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3106 3287 3450 3106
Size: 181 163 4814
Fragments arranged by size:
4814 181 163
BtsI GCAGTG_nn'
Cuts at: 255 351 357 447 504 513 881 2399 255
Size: 96 6 90 57 9 368 1518 3014
Fragments arranged by size:
3014 1518 368 96 90 57 9 6
DraI TTT'AAA
Cuts at: 2155 2155
Size: 5158
DrdI GACnn_nn'nnGTC
Cuts at: 2862 3722 2862
Size: 860 4298
EaeI y'GGCC_r
Cuts at: 230 560 604 1003 1217 1388 1703 1724 1772
Size: 330 44 399 214 171 315 21 48
Cuts at: 1772 2741 2915 3306 3333 4680 230
Size: 969 174 391 27 1347 708
Fragments arranged by size:
1347 969 708 399 391 330 315 214
174 171 48 44 27 21
EagI C'GGCC_G
Cuts at: 604 1703 2741 604
Size: 1099 1038 3021
Fragments arranged by size:
3021 1099 1038
EarI CTCTTCn'nnn_
Cuts at: 277 1364 3179 3389 277
Size: 1087 1815 210 2046
Fragments arranged by size:
2046 1815 1087 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 734 1046 1522 1825 3364 3676 3822 4968 734
Size: 312 476 303 1539 312 146 1146 924
Fragments arranged by size:
1539 1146 924 476 312 312 303 146
EcoICRI GAG'CTC
Cuts at: 2168 2168
Size: 5158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 731 804 954 1117 1171 1204 1396 1501
Size: 574 73 150 163 54 33 192 105
Cuts at: 1501 1513 1602 1660 1963 2044 2981 3413 4162
Size: 12 89 58 303 81 937 432 749
Cuts at: 4162 4430 157
Size: 268 885
Fragments arranged by size:
937 885 749 574 432 303 268 192
163 150 105 89 81 73 58 54
33 12
EcoO109I rG'GnC_Cy
Cuts at: 171 699 700 939 947 1287 1839 2295 171
Size: 528 1 239 8 340 552 456 3034
Fragments arranged by size:
3034 552 528 456 340 239 8 1
EcoRI G'AATT_C
Cuts at: 2160 2160
Size: 5158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 5158
FspI TGC'GCA
Cuts at: 2937 2937
Size: 5158
HaeII r_GCGC'y
Cuts at: 1687 1905 1932 2590 2838 3862 4841 1687
Size: 218 27 658 248 1024 979 2004
Fragments arranged by size:
2004 1024 979 658 248 218 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 729 761 1743 1775 2998 3028 3030
Size: 32 126 32 982 32 1223 30 2
Cuts at: 3030 3060 571
Size: 30 2669
Fragments arranged by size:
2669 1223 982 126 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2195 2195
Size: 5158
KasI G'GCGC_C
Cuts at: 2586 2834 2586
Size: 248 4910
KpnI G_GTAC'C
Cuts at: 296 2176 296
Size: 1880 3278
MluI A'CGCG_T
Cuts at: 4591 4591
Size: 5158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3829 4013 3829
Size: 184 4974
MscI TGG'CCA
Cuts at: 232 562 1726 2917 232
Size: 330 1164 1191 2473
Fragments arranged by size:
2473 1191 1164 330
MslI CAynn'nnrTG
Cuts at: 452 3145 3272 452
Size: 2693 127 2338
Fragments arranged by size:
2693 2338 127
MspA1I CmG'CkG
Cuts at: 482 751 917 1241 1296 1332 1365 1491 1554
Size: 269 166 324 55 36 33 126 63
Cuts at: 1554 1596 1777 1980 1986 2022 2267 2581 2941
Size: 42 181 203 6 36 245 314 360
Cuts at: 2941 3956 4201 482
Size: 1015 245 1439
Fragments arranged by size:
1439 1015 360 324 314 269 245 245
203 181 166 126 63 55 42 36
36 33 6
NaeI GCC'GGC
Cuts at: 1682 3337 1682
Size: 1655 3503
NarI GG'CG_CC
Cuts at: 2587 2835 2587
Size: 248 4910
NcoI C'CATG_G
Cuts at: 125 233 125
Size: 108 5050
NgoMIV G'CCGG_C
Cuts at: 1680 3335 1680
Size: 1655 3503
NruI TCG'CGA
Cuts at: 3519 3519
Size: 5158
NspI r_CATG'y
Cuts at: 2209 2709 3618 2209
Size: 500 909 3749
Fragments arranged by size:
3749 909 500
PasI CC'CwG_GG
Cuts at: 163 163
Size: 5158
PciI A'CATG_T
Cuts at: 3614 3614
Size: 5158
PflMI CCAn_nnn'nTGG
Cuts at: 754 837 942 1095 1182 2022 754
Size: 83 105 153 87 840 3890
Fragments arranged by size:
3890 840 153 105 87 83
PfoI T'CCnGG_A
Cuts at: 1088 1088
Size: 5158
PmeI GTTT'AAAC
Cuts at: 2155 2155
Size: 5158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4297 4329 4297
Size: 32 5126
PpuMI rG'GwC_Cy
Cuts at: 171 939 947 171
Size: 768 8 4382
Fragments arranged by size:
4382 768 8
PshAI GACnn'nnGTC
Cuts at: 398 398
Size: 5158
PspOMI G'GGCC_C
Cuts at: 699 1838 1892 699
Size: 1139 54 3965
Fragments arranged by size:
3965 1139 54
PspXI vC'TCGA_Gb
Cuts at: 2119 2119
Size: 5158
PstI C_TGCA'G
Cuts at: 1240 1301 1316 1337 1868 1985 2203 2888 1240
Size: 61 15 21 531 117 218 685 3510
Fragments arranged by size:
3510 685 531 218 117 61 21 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5158
PvuII CAG'CTG
Cuts at: 1296 1332 1491 1554 1596 1980 1986 2022 2581
Size: 36 159 63 42 384 6 36 559
Cuts at: 2581 2941 1296
Size: 360 3513
Fragments arranged by size:
3513 559 384 360 159 63 42 36
36 6
RsrII CG'GwC_CG
Cuts at: 3351 3351
Size: 5158
SacI G_AGCT'C
Cuts at: 2170 2170
Size: 5158
SalI G'TCGA_C
Cuts at: 2193 2193
Size: 5158
SanDI GG'GwC_CC
Cuts at: 171 171
Size: 5158
SapI GCTCTTCn'nnn_
Cuts at: 277 1364 3179 3389 277
Size: 1087 1815 210 2046
Fragments arranged by size:
2046 1815 1087 210
SbfI CC_TGCA'GG
Cuts at: 2203 2203
Size: 5158
ScaI AGT'ACT
Cuts at: 4675 4675
Size: 5158
SfcI C'TryA_G
Cuts at: 33 813 825 1236 1249 1291 1297 1312 1333
Size: 780 12 411 13 42 6 15 21
Cuts at: 1333 1864 1981 2199 2884 3879 4070 33
Size: 531 117 218 685 995 191 1121
Fragments arranged by size:
1121 995 780 685 531 411 218 191
117 42 21 15 13 12 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 707 1771 707
Size: 1064 4094
SfoI GGC'GCC
Cuts at: 2588 2836 2588
Size: 248 4910
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5158
SmaI CCC'GGG
Cuts at: 1897 2178 1897
Size: 281 4877
SmlI C'TyrA_G
Cuts at: 1675 2119 2311 3226 3720 3982 1675
Size: 444 192 915 494 262 2851
Fragments arranged by size:
2851 915 494 444 262 192
SnaBI TAC'GTA
Cuts at: 2148 2148
Size: 5158
SpeI A'CTAG_T
Cuts at: 67 597 67
Size: 530 4628
SphI G_CATG'C
Cuts at: 2209 2709 2209
Size: 500 4658
StuI AGG'CCT
Cuts at: 1043 2101 1043
Size: 1058 4100
StyI C'CwwG_G
Cuts at: 125 233 1787 2290 2644 125
Size: 108 1554 503 354 2639
Fragments arranged by size:
2639 1554 503 354 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 560 564 2739 2872 3331 3542 560
Size: 4 2175 133 459 211 2176
Fragments arranged by size:
2176 2175 459 211 133 4
TatI w'GTAC_w
Cuts at: 4673 4673
Size: 5158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 539 571 750 782 1093 1125 1426 1458 2058
Size: 32 179 32 311 32 301 32 600
Cuts at: 2058 2090 4675 4707 539
Size: 32 2585 32 990
Fragments arranged by size:
2585 990 600 311 301 179 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2953 2953
Size: 5158
XbaI T'CTAG_A
Cuts at: 2187 2187
Size: 5158
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 240 285 1018 1641 2651 132
Size: 108 45 733 623 1010 2639
Fragments arranged by size:
2639 1010 733 623 108 45
XhoI C'TCGA_G
Cuts at: 2119 2119
Size: 5158
XmaI C'CCGG_G
Cuts at: 1895 2176 1895
Size: 281 4877
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbsI BbvCI BfrBI BmtI Bpu10I BsgI BsiWI
BsmI BsrGI BstEII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PmlI PsiI PsrI SacII SexAI
SgrAI SrfI SspI SwaI XmnI ZraI