Restriction map for
pF1KSDA0532
(Circular) (Six-base) MAPSORT of: pF1KSDA0532.seq Check: 8853 from: 1 to: 7660
pF1KSDA0532 7660 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1878 3194 4696 1878
Size: 1316 1502 4842
Fragments arranged by size:
4842 1502 1316
Acc65I G'GTAC_C
Cuts at: 3700 4674 3700
Size: 974 6686
AclI AA'CG_TT
Cuts at: 7500 7500
Size: 7660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 276 450 1277 2104 3588 3920 4144 5483
Size: 132 174 827 827 1484 332 224 1339
Cuts at: 5483 5915 6664 6932 144
Size: 432 749 268 872
Fragments arranged by size:
1484 1339 872 827 827 749 432 332
268 224 174 132
AflIII A'CryG_T
Cuts at: 173 5010 6116 7093 173
Size: 4837 1106 977 740
Fragments arranged by size:
4837 1106 977 740
AgeI A'CCGG_T
Cuts at: 6048 6048
Size: 7660
AhdI GACnn_n'nnGTC
Cuts at: 4180 4472 4180
Size: 292 7368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3880 3880
Size: 7660
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 183 205 215 237 183
Size: 22 10 22 7606
Fragments arranged by size:
7606 22 22 10
AlwNI CAG_nnn'CTG
Cuts at: 2161 3843 4167 4963 6532 2161
Size: 1682 324 796 1569 3289
Fragments arranged by size:
3289 1682 1569 796 324
ApaI G_GGCC'C
Cuts at: 1548 3510 3637 1548
Size: 1962 127 5571
Fragments arranged by size:
5571 1962 127
ApaLI G'TGCA_C
Cuts at: 1053 1693 3475 4328 6430 1053
Size: 640 1782 853 2102 2283
Fragments arranged by size:
2283 2102 1782 853 640
ApoI r'AATT_y
Cuts at: 1384 2241 2429 3360 4603 4662 7013 7269 1384
Size: 857 188 931 1243 59 2351 256 1775
Fragments arranged by size:
2351 1775 1243 931 857 256 188 59
AseI AT'TA_AT
Cuts at: 20 3137 20
Size: 3117 4543
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7660
AvaI C'yCGr_G
Cuts at: 2236 2339 2853 3629 3811 3966 4678 2236
Size: 103 514 776 182 155 712 5218
Fragments arranged by size:
5218 776 712 514 182 155 103
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 160 193 160
Size: 33 7627
BamHI G'GATC_C
Cuts at: 4683 4683
Size: 7660
BanI G'GyrC_C
Cuts at: 1017 3700 4674 5088 5336 5371 1017
Size: 2683 974 414 248 35 3306
Fragments arranged by size:
3306 2683 974 414 248 35
BanII G_rGCy'C
Cuts at: 1548 3510 3637 4219 4672 5702 1548
Size: 1962 127 582 453 1030 3506
Fragments arranged by size:
3506 1962 1030 582 453 127
BbeI G_GCGC'C
Cuts at: 5092 5340 5092
Size: 248 7412
BbsI GAAGACnn'nnnn_
Cuts at: 456 774 962 1904 2333 2652 3188 4584 456
Size: 318 188 942 429 319 536 1396 3532
Fragments arranged by size:
3532 1396 942 536 429 319 318 188
BciVI GTATCCnnnnn_n'
Cuts at: 516 5556 6319 7660 516
Size: 5040 763 1341 516
Fragments arranged by size:
5040 1341 763 516
BclI T'GATC_A
Cuts at: 3891 5178 3891
Size: 1287 6373
BfrBI ATG'CAT
Cuts at: 2110 2110
Size: 7660
BglI GCCn_nnn'nGGC
Cuts at: 3505 3505
Size: 7660
BglII A'GATC_T
Cuts at: 2918 5173 7212 2918
Size: 2255 2039 3366
Fragments arranged by size:
3366 2255 2039
BlpI GC'TnA_GC
Cuts at: 2807 4022 4770 2807
Size: 1215 748 5697
Fragments arranged by size:
5697 1215 748
Bme1580I G_kGCm'C
Cuts at: 1057 1548 1697 3479 3510 3637 4332 5283 5376
Size: 491 149 1782 31 127 695 951 93
Cuts at: 5376 6434 1057
Size: 1058 2283
Fragments arranged by size:
2283 1782 1058 951 695 491 149 127
93 31
BmgBI CAC'GTC
Cuts at: 4200 4200
Size: 7660
BmrI ACTGGGnnnn_n'
Cuts at: 1683 4870 4951 5286 7432 1683
Size: 3187 81 335 2146 1911
Fragments arranged by size:
3187 2146 1911 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6025 6025
Size: 7660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1376 2199 3645 3659 1376
Size: 823 1446 14 5377
Fragments arranged by size:
5377 1446 823 14
Bpu10I CC'TnA_GC
Cuts at: 4218 4586 4218
Size: 368 7292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 215 1067 3373 4354 4834 5713 6207 6505 215
Size: 852 2306 981 480 879 494 298 1370
Fragments arranged by size:
2306 1370 981 879 852 494 480 298
BsaI GGTCTCn'nnnn_
Cuts at: 3542 7364 3542
Size: 3822 3838
BsaAI yAC'GTr
Cuts at: 3177 4650 5011 3177
Size: 1473 361 5826
Fragments arranged by size:
5826 1473 361
BsaBI GATnn'nnATC
Cuts at: 6 757 5172 7211 6
Size: 751 4415 2039 455
Fragments arranged by size:
4415 2039 751 455
BsaHI Gr'CG_yC
Cuts at: 3656 5089 5337 6041 7537 3656
Size: 1433 248 704 1496 3779
Fragments arranged by size:
3779 1496 1433 704 248
BsaWI w'CCGG_w
Cuts at: 2484 4847 5368 6048 6322 6469 6880 2484
Size: 2363 521 680 274 147 411 3264
Fragments arranged by size:
3264 2363 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 205 235 1349 1379 3632 3662 205
Size: 30 1114 30 2253 30 4203
Fragments arranged by size:
4203 2253 1114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 469 1143 3031 3407 3784 3805 4028 469
Size: 674 1888 376 377 21 223 4101
Fragments arranged by size:
4101 1888 674 377 376 223 21
BseYI C'CCAG_C
Cuts at: 2069 2136 3502 3524 3852 4486 5083 5841 6420
Size: 67 1366 22 328 634 597 758 579
Cuts at: 6420 6916 2069
Size: 496 2813
Fragments arranged by size:
2813 1366 758 634 597 579 496 328
67 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 333 1322 4384 276
Size: 57 989 3062 3552
Fragments arranged by size:
3552 3062 989 57
BsiEI CG_ry'CG
Cuts at: 104 5200 5246 6456 104
Size: 5096 46 1210 1308
Fragments arranged by size:
5096 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1057 1697 2446 3479 3776 4265 4332 4672 5450
Size: 640 749 1033 297 489 67 340 778
Cuts at: 5450 5640 6434 1057
Size: 190 794 2283
Fragments arranged by size:
2283 1033 794 778 749 640 489 340
297 190 67
BsmBI CGTCTCn'nnnn_
Cuts at: 3663 3663
Size: 7660
Bsp1286I G_dGCh'C
Cuts at: 1057 1548 1697 2446 3479 3510 3637 3776 4219
Size: 491 149 749 1033 31 127 139 443
Cuts at: 4219 4265 4332 4672 5283 5376 5450 5640 5702
Size: 46 67 340 611 93 74 190 62
Cuts at: 5702 6434 1057
Size: 732 2283
Fragments arranged by size:
2283 1033 749 732 611 491 443 340
190 149 139 127 93 74 67 62
46 31
BspEI T'CCGG_A
Cuts at: 2484 6880 2484
Size: 4396 3264
BspHI T'CATG_A
Cuts at: 1124 2454 6836 1124
Size: 1330 4382 1948
Fragments arranged by size:
4382 1948 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2942 3171 3227 3282 4708 4922 5224 5605 2942
Size: 229 56 55 1426 214 302 381 4997
Fragments arranged by size:
4997 1426 381 302 229 214 56 55
BsrBI CCG'CTC
Cuts at: 50 5950 6004 7490 7655 50
Size: 5900 54 1486 165 55
Fragments arranged by size:
5900 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3494 5570 3494
Size: 2076 5584
BsrFI r'CCGG_y
Cuts at: 3752 3782 5656 5837 6048 3752
Size: 30 1874 181 211 5364
Fragments arranged by size:
5364 1874 211 181 30
BsrGI T'GTAC_A
Cuts at: 1251 2640 3395 1251
Size: 1389 755 5516
Fragments arranged by size:
5516 1389 755
BssSI C'ACGA_G
Cuts at: 5929 6289 5929
Size: 360 7300
BstAPI GCAn_nnn'nTGC
Cuts at: 3474 5035 3474
Size: 1561 6099
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7660
BstXI CCAn_nnnn'nTGG
Cuts at: 3315 3735 3315
Size: 420 7240
BstYI r'GATC_y
Cuts at: 1 215 1373 2918 4683 5150 5173 5508 5754
Size: 214 1158 1545 1765 467 23 335 246
Cuts at: 5754 6768 6854 6866 7212 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1765 1545 1158 1014 467 449 346 335
246 214 86 23 12
BstZ17I GTA'TAC
Cuts at: 1879 3195 1879
Size: 1316 6344
Bsu36I CC'TnA_GG
Cuts at: 446 4630 446
Size: 4184 3476
BtgI C'CryG_G
Cuts at: 1862 4610 1862
Size: 2748 4912
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 338 5608 5789 5952 338
Size: 5270 181 163 2046
Fragments arranged by size:
5270 2046 181 163
BtsI GCAGTG_nn'
Cuts at: 410 502 1225 2777 3458 4901 410
Size: 92 723 1552 681 1443 3169
Fragments arranged by size:
3169 1552 1443 723 681 92
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3660 3695 3660
Size: 35 7625
DraI TTT'AAA
Cuts at: 2742 4657 2742
Size: 1915 5745
DrdI GACnn_nn'nnGTC
Cuts at: 5364 6224 5364
Size: 860 6800
EaeI y'GGCC_r
Cuts at: 513 5243 5417 5808 5835 7182 513
Size: 4730 174 391 27 1347 991
Fragments arranged by size:
4730 1347 991 391 174 27
EagI C'GGCC_G
Cuts at: 5243 5243
Size: 7660
EarI CTCTTCn'nnn_
Cuts at: 2873 4270 5681 5891 2873
Size: 1397 1411 210 4642
Fragments arranged by size:
4642 1411 1397 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5866 6178 6324 7470 5866
Size: 312 146 1146 6056
Fragments arranged by size:
6056 1146 312 146
EcoICRI GAG'CTC
Cuts at: 4670 4670
Size: 7660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 276 450 1277 1376 2104 2199 3588 3645
Size: 132 174 827 99 728 95 1389 57
Cuts at: 3645 3659 3920 4144 5483 5915 6664 6932 144
Size: 14 261 224 1339 432 749 268 872
Fragments arranged by size:
1389 1339 872 827 749 728 432 268
261 224 174 132 99 95 57 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 3823 2153
Size: 1670 5990
EcoO109I rG'GnC_Cy
Cuts at: 1545 1572 2692 2931 3311 3445 3506 3507 3633
Size: 27 1120 239 380 134 61 1 126
Cuts at: 3633 3634 3840 4797 1545
Size: 1 206 957 4408
Fragments arranged by size:
4408 1120 957 380 239 206 134 126
61 27 1 1
EcoRI G'AATT_C
Cuts at: 1384 4662 1384
Size: 3278 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2524 5677 2524
Size: 3153 4507
FspI TGC'GCA
Cuts at: 5439 5439
Size: 7660
HaeII r_GCGC'y
Cuts at: 579 2722 4092 5092 5340 6364 7343 579
Size: 2143 1370 1000 248 1024 979 896
Fragments arranged by size:
2143 1370 1024 1000 979 896 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 761 793 1119 1151 2333 2365 3434 3466 3742
Size: 32 326 32 1182 32 1069 32 276
Cuts at: 3742 3774 4015 4047 4166 4198 4408 4440 4458
Size: 32 241 32 119 32 210 32 18
Cuts at: 4458 4490 5500 5530 5532 5562 761
Size: 32 1010 30 2 30 2859
Fragments arranged by size:
2859 1182 1069 1010 326 276 241 210
119 32 32 32 32 32 32 32
32 32 30 30 18 2
HincII GTy'rAC
Cuts at: 4697 4697
Size: 7660
HindIII A'AGCT_T
Cuts at: 422 1652 422
Size: 1230 6430
KasI G'GCGC_C
Cuts at: 5088 5336 5088
Size: 248 7412
KpnI G_GTAC'C
Cuts at: 3704 4678 3704
Size: 974 6686
MfeI C'AATT_G
Cuts at: 3875 3875
Size: 7660
MluI A'CGCG_T
Cuts at: 7093 7093
Size: 7660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3041 3074 6331 6515 3041
Size: 33 3257 184 4186
Fragments arranged by size:
4186 3257 184 33
MscI TGG'CCA
Cuts at: 515 5419 515
Size: 4904 2756
MslI CAynn'nnrTG
Cuts at: 1015 2648 2668 3991 5647 5774 1015
Size: 1633 20 1323 1656 127 2901
Fragments arranged by size:
2901 1656 1633 1323 127 20
MspA1I CmG'CkG
Cuts at: 572 597 2806 3502 4490 4769 5083 5443 6458
Size: 25 2209 696 988 279 314 360 1015
Cuts at: 6458 6703 572
Size: 245 1529
Fragments arranged by size:
2209 1529 1015 988 696 360 314 279
245 25
NaeI GCC'GGC
Cuts at: 5839 5839
Size: 7660
NarI GG'CG_CC
Cuts at: 5089 5337 5089
Size: 248 7412
NcoI C'CATG_G
Cuts at: 1862 4610 1862
Size: 2748 4912
NdeI CA'TA_TG
Cuts at: 1417 1509 1417
Size: 92 7568
NgoMIV G'CCGG_C
Cuts at: 5837 5837
Size: 7660
NruI TCG'CGA
Cuts at: 6021 6021
Size: 7660
NsiI A_TGCA'T
Cuts at: 2112 2112
Size: 7660
NspI r_CATG'y
Cuts at: 177 394 4114 4711 5211 6120 177
Size: 217 3720 597 500 909 1717
Fragments arranged by size:
3720 1717 909 597 500 217
PasI CC'CwG_GG
Cuts at: 1541 2872 1541
Size: 1331 6329
PciI A'CATG_T
Cuts at: 173 6116 173
Size: 5943 1717
PflMI CCAn_nnn'nTGG
Cuts at: 4566 4566
Size: 7660
PfoI T'CCnGG_A
Cuts at: 1203 1824 2246 1203
Size: 621 422 6617
Fragments arranged by size:
6617 621 422
PmeI GTTT'AAAC
Cuts at: 4657 4657
Size: 7660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 205 237 693 725 6799 6831 205
Size: 32 456 32 6074 32 1034
Fragments arranged by size:
6074 1034 456 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1572 2692 2931 3445 1572
Size: 1120 239 514 5787
Fragments arranged by size:
5787 1120 514 239
PshAI GACnn'nnGTC
Cuts at: 2090 2333 2090
Size: 243 7417
PsiI TTA'TAA
Cuts at: 1943 2380 2957 1943
Size: 437 577 6646
Fragments arranged by size:
6646 577 437
PspOMI G'GGCC_C
Cuts at: 1544 3506 3633 1544
Size: 1962 127 5571
Fragments arranged by size:
5571 1962 127
PspXI vC'TCGA_Gb
Cuts at: 2339 2339
Size: 7660
PstI C_TGCA'G
Cuts at: 2939 4530 4705 5390 2939
Size: 1591 175 685 5209
Fragments arranged by size:
5209 1591 685 175
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7660
PvuII CAG'CTG
Cuts at: 572 597 2806 4490 5083 5443 572
Size: 25 2209 1684 593 360 2789
Fragments arranged by size:
2789 2209 1684 593 360 25
RsrII CG'GwC_CG
Cuts at: 5853 5853
Size: 7660
SacI G_AGCT'C
Cuts at: 4672 4672
Size: 7660
SalI G'TCGA_C
Cuts at: 4695 4695
Size: 7660
SapI GCTCTTCn'nnn_
Cuts at: 2873 4270 5681 5891 2873
Size: 1397 1411 210 4642
Fragments arranged by size:
4642 1411 1397 210
SbfI CC_TGCA'GG
Cuts at: 4705 4705
Size: 7660
ScaI AGT'ACT
Cuts at: 2102 7177 2102
Size: 5075 2585
SexAI A'CCwGG_T
Cuts at: 4565 4565
Size: 7660
SfcI C'TryA_G
Cuts at: 33 780 848 1606 2192 2273 2935 4526 4701
Size: 747 68 758 586 81 662 1591 175
Cuts at: 4701 5386 6381 6572 33
Size: 685 995 191 1121
Fragments arranged by size:
1591 1121 995 758 747 685 662 586
191 175 81 68
SfoI GGC'GCC
Cuts at: 5090 5338 5090
Size: 248 7412
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7660
SmaI CCC'GGG
Cuts at: 2855 3631 4680 2855
Size: 776 1049 5835
Fragments arranged by size:
5835 1049 776
SmlI C'TyrA_G
Cuts at: 230 1082 2339 3388 4369 4813 5728 6222 6484
Size: 852 1257 1049 981 444 915 494 262
Cuts at: 6484 230
Size: 1406
Fragments arranged by size:
1406 1257 1049 981 915 852 494 444
262
SnaBI TAC'GTA
Cuts at: 4650 4650
Size: 7660
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7660
SphI G_CATG'C
Cuts at: 4711 5211 4711
Size: 500 7160
SspI AAT'ATT
Cuts at: 537 1937 537
Size: 1400 6260
StuI AGG'CCT
Cuts at: 4303 4303
Size: 7660
StyI C'CwwG_G
Cuts at: 381 1862 3314 3953 4610 4792 5146 381
Size: 1481 1452 639 657 182 354 2895
Fragments arranged by size:
2895 1481 1452 657 639 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2803 5241 5374 5833 6044 2803
Size: 2438 133 459 211 4419
Fragments arranged by size:
4419 2438 459 211 133
TatI w'GTAC_w
Cuts at: 566 689 741 998 1251 2100 2640 3395 4436
Size: 123 52 257 253 849 540 755 1041
Cuts at: 4436 7175 566
Size: 2739 1051
Fragments arranged by size:
2739 1051 1041 849 755 540 257 253
123 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3210 3242 3632 3664 7177 7209 3210
Size: 32 390 32 3513 32 3661
Fragments arranged by size:
3661 3513 390 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5455 5455
Size: 7660
XbaI T'CTAG_A
Cuts at: 249 2538 4689 249
Size: 2289 2151 3220
Fragments arranged by size:
3220 2289 2151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 2220 3057 5153 327
Size: 1893 837 2096 2834
Fragments arranged by size:
2834 2096 1893 837
XhoI C'TCGA_G
Cuts at: 2339 2339
Size: 7660
XmaI C'CCGG_G
Cuts at: 2853 3629 4678 2853
Size: 776 1049 5835
Fragments arranged by size:
5835 1049 776
XmnI GAAnn'nnTTC
Cuts at: 289 536 705 1655 3014 289
Size: 247 169 950 1359 4935
Fragments arranged by size:
4935 1359 950 247 169
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AscI AvrII BbvCI
BcgI BmtI BsiWI BsmI BssHII BstEII ClaI DraIII
EcoRV FseI FspAI HpaI NheI NotI PacI PmlI
PsrI SacII SanDI SfiI SgrAI SrfI SwaI ZraI