Restriction map for
pF1KSDA0526
(Circular) (Six-base) MAPSORT of: pF1KSDA0526.seq Check: 6274 from: 1 to: 4825
pF1KSDA0526 4825 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 610 1861 610
Size: 1251 3574
Acc65I G'GTAC_C
Cuts at: 1758 1839 1758
Size: 81 4744
AclI AA'CG_TT
Cuts at: 1252 4665 1252
Size: 3413 1412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1606 1756 2648 3080 3829 4097 1130
Size: 476 150 892 432 749 268 1858
Fragments arranged by size:
1858 892 749 476 432 268 150
AfeI AGC'GCT
Cuts at: 225 225
Size: 4825
AflIII A'CryG_T
Cuts at: 2175 3281 4258 2175
Size: 1106 977 2742
Fragments arranged by size:
2742 1106 977
AgeI A'CCGG_T
Cuts at: 3213 3213
Size: 4825
AhdI GACnn_n'nnGTC
Cuts at: 459 459
Size: 4825
AleI CACnn'nnGTG
Cuts at: 349 349
Size: 4825
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1082 1114 1082
Size: 32 4793
AlwNI CAG_nnn'CTG
Cuts at: 2128 3697 2128
Size: 1569 3256
ApaI G_GGCC'C
Cuts at: 1186 1186
Size: 4825
ApaLI G'TGCA_C
Cuts at: 3595 3595
Size: 4825
ApoI r'AATT_y
Cuts at: 849 1052 1827 4178 4434 849
Size: 203 775 2351 256 1240
Fragments arranged by size:
2351 1240 775 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 4825
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4825
AvaI C'yCGr_G
Cuts at: 1225 1843 1225
Size: 618 4207
BamHI G'GATC_C
Cuts at: 1221 1848 1221
Size: 627 4198
BanI G'GyrC_C
Cuts at: 1174 1417 1564 1758 1839 2253 2501 2536 1174
Size: 243 147 194 81 414 248 35 3463
Fragments arranged by size:
3463 414 248 243 194 147 81 35
BanII G_rGCy'C
Cuts at: 140 1186 1837 2867 140
Size: 1046 651 1030 2098
Fragments arranged by size:
2098 1046 1030 651
BbeI G_GCGC'C
Cuts at: 1178 1568 2257 2505 1178
Size: 390 689 248 3498
Fragments arranged by size:
3498 689 390 248
BbsI GAAGACnn'nnnn_
Cuts at: 1096 1523 1814 1096
Size: 427 291 4107
Fragments arranged by size:
4107 427 291
BciVI GTATCCnnnnn_n'
Cuts at: 2721 3484 4825 2721
Size: 763 1341 2721
Fragments arranged by size:
2721 1341 763
BclI T'GATC_A
Cuts at: 2343 2343
Size: 4825
BfrBI ATG'CAT
Cuts at: 916 916
Size: 4825
BglI GCCn_nnn'nGGC
Cuts at: 1190 1190
Size: 4825
BglII A'GATC_T
Cuts at: 2338 4377 2338
Size: 2039 2786
BlpI GC'TnA_GC
Cuts at: 1935 1935
Size: 4825
Bme1580I G_kGCm'C
Cuts at: 1186 2448 2541 3599 1186
Size: 1262 93 1058 2412
Fragments arranged by size:
2412 1262 1058 93
BmgBI CAC'GTC
Cuts at: 1352 1352
Size: 4825
BmrI ACTGGGnnnn_n'
Cuts at: 2035 2116 2451 4597 2035
Size: 81 335 2146 2263
Fragments arranged by size:
2263 2146 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 599 3190 599
Size: 2591 2234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 749 1298 1508 572
Size: 177 549 210 3889
Fragments arranged by size:
3889 549 210 177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 733 1108 1999 2878 3372 3670 417
Size: 316 375 891 879 494 298 1572
Fragments arranged by size:
1572 891 879 494 375 316 298
BsaI GGTCTCn'nnnn_
Cuts at: 4529 4529
Size: 4825
BsaAI yAC'GTr
Cuts at: 213 347 1815 2176 213
Size: 134 1468 361 2862
Fragments arranged by size:
2862 1468 361 134
BsaBI GATnn'nnATC
Cuts at: 6 2337 4376 6
Size: 2331 2039 455
Fragments arranged by size:
2331 2039 455
BsaHI Gr'CG_yC
Cuts at: 1175 1479 1565 2254 2502 3206 4702 1175
Size: 304 86 689 248 704 1496 1298
Fragments arranged by size:
1496 1298 704 689 304 248 86
BsaWI w'CCGG_w
Cuts at: 2012 2533 3213 3487 3634 4045 2012
Size: 521 680 274 147 411 2792
Fragments arranged by size:
2792 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 545 565 575 595 716 746 545
Size: 20 10 20 121 30 4624
Fragments arranged by size:
4624 121 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 231 730 799 231
Size: 499 69 4257
Fragments arranged by size:
4257 499 69
BseYI C'CCAG_C
Cuts at: 2248 3006 3585 4081 2248
Size: 758 579 496 2992
Fragments arranged by size:
2992 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 1358 756
Size: 602 4223
BsiEI CG_ry'CG
Cuts at: 104 133 2365 2411 3621 104
Size: 29 2232 46 1210 1308
Fragments arranged by size:
2232 1308 1210 46 29
BsiHKAI G_wGCw'C
Cuts at: 344 1837 2615 2805 3599 344
Size: 1493 778 190 794 1570
Fragments arranged by size:
1570 1493 794 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1470 1780 1470
Size: 310 4515
Bsp1286I G_dGCh'C
Cuts at: 140 344 1186 1837 2448 2541 2615 2805 2867
Size: 204 842 651 611 93 74 190 62
Cuts at: 2867 3599 140
Size: 732 1366
Fragments arranged by size:
1366 842 732 651 611 204 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4045 4045
Size: 4825
BspHI T'CATG_A
Cuts at: 585 4001 585
Size: 3416 1409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1325 1873 2087 2389 2770 1325
Size: 548 214 302 381 3380
Fragments arranged by size:
3380 548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3115 3169 4655 4820 50
Size: 3065 54 1486 165 55
Fragments arranged by size:
3065 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2735 2735
Size: 4825
BsrFI r'CCGG_y
Cuts at: 250 2821 3002 3213 250
Size: 2571 181 211 1862
Fragments arranged by size:
2571 1862 211 181
BsrGI T'GTAC_A
Cuts at: 503 1440 503
Size: 937 3888
BssSI C'ACGA_G
Cuts at: 3094 3454 3094
Size: 360 4465
BstAPI GCAn_nnn'nTGC
Cuts at: 884 1345 2200 884
Size: 461 855 3509
Fragments arranged by size:
3509 855 461
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4825
BstYI r'GATC_y
Cuts at: 1 257 1090 1221 1848 2315 2338 2673 2919
Size: 256 833 131 627 467 23 335 246
Cuts at: 2919 3933 4019 4031 4377 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 833 627 467 449 346 335 256
246 131 86 23 12
BstZ17I GTA'TAC
Cuts at: 611 611
Size: 4825
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2773 2954 3117 2773
Size: 181 163 4481
Fragments arranged by size:
4481 181 163
BtsI GCAGTG_nn'
Cuts at: 1346 2066 1346
Size: 720 4105
DraI TTT'AAA
Cuts at: 1700 1822 1700
Size: 122 4703
DrdI GACnn_nn'nnGTC
Cuts at: 2529 3389 2529
Size: 860 3965
EaeI y'GGCC_r
Cuts at: 130 252 532 2408 2582 2973 3000 4347 130
Size: 122 280 1876 174 391 27 1347 608
Fragments arranged by size:
1876 1347 608 391 280 174 122 27
EagI C'GGCC_G
Cuts at: 130 2408 130
Size: 2278 2547
EarI CTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3031 3343 3489 4635 3031
Size: 312 146 1146 3221
Fragments arranged by size:
3221 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1835 1835
Size: 4825
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 572 749 1130 1298 1508 1606 1756 2648 3080
Size: 177 381 168 210 98 150 892 432
Cuts at: 3080 3829 4097 572
Size: 749 268 1300
Fragments arranged by size:
1300 892 749 432 381 268 210 177
168 150 98
EcoO109I rG'GnC_Cy
Cuts at: 1040 1183 1722 1775 1962 1040
Size: 143 539 53 187 3903
Fragments arranged by size:
3903 539 187 143 53
EcoRI G'AATT_C
Cuts at: 849 1827 849
Size: 978 3847
EcoRV GAT'ATC
Cuts at: 382 382
Size: 4825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1161 1702 2842 1161
Size: 541 1140 3144
Fragments arranged by size:
3144 1140 541
FspI TGC'GCA
Cuts at: 2604 2604
Size: 4825
HaeII r_GCGC'y
Cuts at: 227 1178 1568 2257 2505 3529 4508 227
Size: 951 390 689 248 1024 979 544
Fragments arranged by size:
1024 979 951 689 544 390 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 445 477 565 597 2665 2695 2697 2727 445
Size: 32 88 32 2068 30 2 30 2543
Fragments arranged by size:
2543 2068 88 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1862 1862
Size: 4825
HindIII A'AGCT_T
Cuts at: 308 308
Size: 4825
KasI G'GCGC_C
Cuts at: 1174 1564 2253 2501 1174
Size: 390 689 248 3498
Fragments arranged by size:
3498 689 390 248
KpnI G_GTAC'C
Cuts at: 1762 1843 1762
Size: 81 4744
MluI A'CGCG_T
Cuts at: 4258 4258
Size: 4825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1593 3496 3680 1593
Size: 1903 184 2738
Fragments arranged by size:
2738 1903 184
MscI TGG'CCA
Cuts at: 2584 2584
Size: 4825
MslI CAynn'nnrTG
Cuts at: 349 1063 1390 2812 2939 349
Size: 714 327 1422 127 2235
Fragments arranged by size:
2235 1422 714 327 127
MspA1I CmG'CkG
Cuts at: 1734 1934 2248 2608 3623 3868 1734
Size: 200 314 360 1015 245 2691
Fragments arranged by size:
2691 1015 360 314 245 200
NaeI GCC'GGC
Cuts at: 252 3004 252
Size: 2752 2073
NarI GG'CG_CC
Cuts at: 1175 1565 2254 2502 1175
Size: 390 689 248 3498
Fragments arranged by size:
3498 689 390 248
NgoMIV G'CCGG_C
Cuts at: 250 3002 250
Size: 2752 2073
NruI TCG'CGA
Cuts at: 3186 3186
Size: 4825
NsiI A_TGCA'T
Cuts at: 918 918
Size: 4825
NspI r_CATG'y
Cuts at: 1551 1876 2376 3285 1551
Size: 325 500 909 3091
Fragments arranged by size:
3091 909 500 325
PasI CC'CwG_GG
Cuts at: 1179 1179
Size: 4825
PciI A'CATG_T
Cuts at: 3281 3281
Size: 4825
PmeI GTTT'AAAC
Cuts at: 1822 1822
Size: 4825
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1314 1346 3964 3996 1314
Size: 32 2618 32 2143
Fragments arranged by size:
2618 2143 32 32
PpuMI rG'GwC_Cy
Cuts at: 1722 1722
Size: 4825
PsiI TTA'TAA
Cuts at: 593 637 593
Size: 44 4781
PspOMI G'GGCC_C
Cuts at: 1182 1182
Size: 4825
PstI C_TGCA'G
Cuts at: 231 1870 2555 231
Size: 1639 685 2501
Fragments arranged by size:
2501 1639 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4825
PvuII CAG'CTG
Cuts at: 1734 2248 2608 1734
Size: 514 360 3951
Fragments arranged by size:
3951 514 360
RsrII CG'GwC_CG
Cuts at: 3018 3018
Size: 4825
SacI G_AGCT'C
Cuts at: 1837 1837
Size: 4825
SalI G'TCGA_C
Cuts at: 1860 1860
Size: 4825
SapI GCTCTTCn'nnn_
Cuts at: 2846 3056 2846
Size: 210 4615
SbfI CC_TGCA'GG
Cuts at: 1870 1870
Size: 4825
ScaI AGT'ACT
Cuts at: 741 1210 4342 741
Size: 469 3132 1224
Fragments arranged by size:
3132 1224 469
SexAI A'CCwGG_T
Cuts at: 1462 1462
Size: 4825
SfcI C'TryA_G
Cuts at: 33 227 540 1367 1866 2551 3546 3737 33
Size: 194 313 827 499 685 995 191 1121
Fragments arranged by size:
1121 995 827 685 499 313 194 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1190 1190
Size: 4825
SfoI GGC'GCC
Cuts at: 1176 1566 2255 2503 1176
Size: 390 689 248 3498
Fragments arranged by size:
3498 689 390 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4825
SmaI CCC'GGG
Cuts at: 1845 1845
Size: 4825
SmlI C'TyrA_G
Cuts at: 396 712 1123 1978 2893 3387 3649 396
Size: 316 411 855 915 494 262 1572
Fragments arranged by size:
1572 915 855 494 411 316 262
SnaBI TAC'GTA
Cuts at: 1815 1815
Size: 4825
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4825
SphI G_CATG'C
Cuts at: 1876 2376 1876
Size: 500 4325
StyI C'CwwG_G
Cuts at: 1425 1957 2311 1425
Size: 532 354 3939
Fragments arranged by size:
3939 532 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2406 2539 2998 3209 2406
Size: 133 459 211 4022
Fragments arranged by size:
4022 459 211 133
TatI w'GTAC_w
Cuts at: 503 739 1208 1440 4340 503
Size: 236 469 232 2900 988
Fragments arranged by size:
2900 988 469 236 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 545 577 716 748 1584 1616 4342 4374 545
Size: 32 139 32 836 32 2726 32 996
Fragments arranged by size:
2726 996 836 139 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2620 2620
Size: 4825
XbaI T'CTAG_A
Cuts at: 1854 1854
Size: 4825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2318 2318
Size: 4825
XmaI C'CCGG_G
Cuts at: 1843 1843
Size: 4825
XmnI GAAnn'nnTTC
Cuts at: 1326 1326
Size: 4825
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NruI NsiI NspI PasI PciI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AscI AvrII BaeI BbvCI
BcgI BmtI Bpu10I BsiWI BsmI BssHII BstEII BstXI
Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NcoI NdeI NheI NotI PacI PflMI
PfoI PmlI PshAI PspXI PsrI SacII SanDI SgrAI
SrfI SspI StuI SwaI XhoI ZraI