Restriction map for
pF1KSDA0482
(Circular) (Six-base) MAPSORT of: pF1KSDA0482.seq Check: 3557 from: 1 to: 7009
pF1KSDA0482 7009 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 636 636
Size: 7009
AccI GT'mk_AC
Cuts at: 821 4045 821
Size: 3224 3785
Acc65I G'GTAC_C
Cuts at: 877 1061 2033 4023 877
Size: 184 972 1990 3863
Fragments arranged by size:
3863 1990 972 184
AclI AA'CG_TT
Cuts at: 6849 6849
Size: 7009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 2280 2696 2975 3613 4832 5264 6013 6281
Size: 1529 416 279 638 1219 432 749 268
Cuts at: 6281 751
Size: 1479
Fragments arranged by size:
1529 1479 1219 749 638 432 416 279
268
AfeI AGC'GCT
Cuts at: 2180 2647 2180
Size: 467 6542
AflIII A'CryG_T
Cuts at: 713 866 1532 4359 5465 6442 713
Size: 153 666 2827 1106 977 1280
Fragments arranged by size:
2827 1280 1106 977 666 153
AgeI A'CCGG_T
Cuts at: 5397 5397
Size: 7009
AhdI GACnn_n'nnGTC
Cuts at: 480 480
Size: 7009
AleI CACnn'nnGTG
Cuts at: 1128 2836 1128
Size: 1708 5301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1146 4002 1146
Size: 2856 4153
AlwNI CAG_nnn'CTG
Cuts at: 494 793 1590 2113 2146 4312 5881 494
Size: 299 797 523 33 2166 1569 1622
Fragments arranged by size:
2166 1622 1569 797 523 299 33
ApaI G_GGCC'C
Cuts at: 215 1119 1299 1833 3155 215
Size: 904 180 534 1322 4069
Fragments arranged by size:
4069 1322 904 534 180
ApaLI G'TGCA_C
Cuts at: 1459 3676 5779 1459
Size: 2217 2103 2689
Fragments arranged by size:
2689 2217 2103
ApoI r'AATT_y
Cuts at: 4011 6362 6618 4011
Size: 2351 256 4402
Fragments arranged by size:
4402 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7009
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7009
AvaI C'yCGr_G
Cuts at: 515 1826 2669 2798 3012 3051 3303 3458 3707
Size: 1311 843 129 214 39 252 155 249
Cuts at: 3707 4027 515
Size: 320 3497
Fragments arranged by size:
3497 1311 843 320 252 249 214 155
129 39
BamHI G'GATC_C
Cuts at: 1047 3507 4032 1047
Size: 2460 525 4024
Fragments arranged by size:
4024 2460 525
BanI G'GyrC_C
Cuts at: 877 1061 1663 2033 2227 3564 4023 4437 4685
Size: 184 602 370 194 1337 459 414 248
Cuts at: 4685 4720 877
Size: 35 3166
Fragments arranged by size:
3166 1337 602 459 414 370 248 194
184 35
BanII G_rGCy'C
Cuts at: 215 334 418 560 896 1119 1299 1833 2239
Size: 119 84 142 336 223 180 534 406
Cuts at: 2239 3101 3155 3310 3319 3331 3714 3815 3955
Size: 862 54 155 9 12 383 101 140
Cuts at: 3955 4021 5051 215
Size: 66 1030 2173
Fragments arranged by size:
2173 1030 862 534 406 383 336 223
180 155 142 140 119 101 84 66
54 12 9
BbeI G_GCGC'C
Cuts at: 1667 4441 4689 1667
Size: 2774 248 3987
Fragments arranged by size:
3987 2774 248
BbsI GAAGACnn'nnnn_
Cuts at: 912 1008 912
Size: 96 6913
BbvCI CC'TCA_GC
Cuts at: 972 2106 2509 2568 3110 3369 3443 972
Size: 1134 403 59 542 259 74 4538
Fragments arranged by size:
4538 1134 542 403 259 74 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2609 2643 3619 3653 2609
Size: 34 976 34 5965
Fragments arranged by size:
5965 976 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 771 1175 4905 5668 7009 771
Size: 404 3730 763 1341 771
Fragments arranged by size:
3730 1341 771 763 404
BclI T'GATC_A
Cuts at: 4527 4527
Size: 7009
BglI GCCn_nnn'nGGC
Cuts at: 2257 2597 3196 2257
Size: 340 599 6070
Fragments arranged by size:
6070 599 340
BglII A'GATC_T
Cuts at: 1769 4522 6561 1769
Size: 2753 2039 2217
Fragments arranged by size:
2753 2217 2039
BlpI GC'TnA_GC
Cuts at: 3179 3470 4119 3179
Size: 291 649 6069
Fragments arranged by size:
6069 649 291
Bme1580I G_kGCm'C
Cuts at: 215 964 1119 1299 1413 1463 1833 2843 2940
Size: 749 155 180 114 50 370 1010 97
Cuts at: 2940 3155 3569 3680 4632 4725 5783 215
Size: 215 414 111 952 93 1058 1441
Fragments arranged by size:
1441 1058 1010 952 749 414 370 215
180 155 114 111 97 93 50
BmgBI CAC'GTC
Cuts at: 752 867 1533 752
Size: 115 666 6228
Fragments arranged by size:
6228 666 115
BmrI ACTGGGnnnn_n'
Cuts at: 1294 2779 3158 3676 3811 4219 4300 4635 6781
Size: 1485 379 518 135 408 81 335 2146
Cuts at: 6781 1294
Size: 1522
Fragments arranged by size:
2146 1522 1485 518 408 379 335 135
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 720 3038 5374 720
Size: 2318 2336 2355
Fragments arranged by size:
2355 2336 2318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 706 751 760 1488 1689 1936 1971 2059
Size: 309 45 9 728 201 247 35 88
Cuts at: 2059 2926 3055 3112 3225 3487 3555 3829 397
Size: 867 129 57 113 262 68 274 3577
Fragments arranged by size:
3577 867 728 309 274 262 247 201
129 113 88 68 57 45 35 9
Bpu10I CC'TnA_GC
Cuts at: 972 2106 2233 2509 2568 3110 3369 3443 972
Size: 1134 127 276 59 542 259 74 4538
Fragments arranged by size:
4538 1134 542 276 259 127 74 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 4183 5062 5556 5854 544
Size: 3639 879 494 298 1699
Fragments arranged by size:
3639 1699 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 373 1110 2516 6713 373
Size: 737 1406 4197 669
Fragments arranged by size:
4197 1406 737 669
BsaAI yAC'GTr
Cuts at: 3498 3999 4360 3498
Size: 501 361 6147
Fragments arranged by size:
6147 501 361
BsaBI GATnn'nnATC
Cuts at: 6 1784 4521 6560 6
Size: 1778 2737 2039 455
Fragments arranged by size:
2737 2039 1778 455
BsaHI Gr'CG_yC
Cuts at: 197 1664 2438 4438 4686 5390 6886 197
Size: 1467 774 2000 248 704 1496 320
Fragments arranged by size:
2000 1496 1467 774 704 320 248
BsaWI w'CCGG_w
Cuts at: 3689 4196 4717 5397 5671 5818 6229 3689
Size: 507 521 680 274 147 411 4469
Fragments arranged by size:
4469 680 521 507 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 328 358 3265 3295 328
Size: 30 2907 30 4042
Fragments arranged by size:
4042 2907 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 333 336 703 748 1046 1978 2073 2076 2079
Size: 3 367 45 298 932 95 3 3
Cuts at: 2079 2094 2786 2816 3011 3109 3895 3946 3949
Size: 15 692 30 195 98 786 51 3
Cuts at: 3949 333
Size: 3393
Fragments arranged by size:
3393 932 786 692 367 298 195 98
95 51 45 30 15 3 3 3
3
BseYI C'CCAG_C
Cuts at: 241 427 1222 1266 1445 1552 1557 1636 2056
Size: 186 795 44 179 107 5 79 420
Cuts at: 2056 2385 2392 2397 2404 2427 2757 2810 2844
Size: 329 7 5 7 23 330 53 34
Cuts at: 2844 3448 4432 5190 5769 6265 241
Size: 604 984 758 579 496 985
Fragments arranged by size:
985 984 795 758 604 579 496 420
330 329 186 179 107 79 53 44
34 23 7 7 5 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1106 2464 3972 670
Size: 436 1358 1508 3707
Fragments arranged by size:
3707 1508 1358 436
BsiEI CG_ry'CG
Cuts at: 104 4549 4595 5805 104
Size: 4445 46 1210 1308
Fragments arranged by size:
4445 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 334 403 560 746 896 1307 1463 2894 3101
Size: 69 157 186 150 411 156 1431 207
Cuts at: 3101 3503 3680 3955 4021 4799 4989 5783 334
Size: 402 177 275 66 778 190 794 1560
Fragments arranged by size:
1560 1431 794 778 411 402 275 207
190 186 177 157 156 150 69 66
Bsp1286I G_dGCh'C
Cuts at: 215 334 403 418 560 746 896 964 1119
Size: 119 69 15 142 186 150 68 155
Cuts at: 1119 1299 1307 1413 1463 1833 2239 2843 2894
Size: 180 8 106 50 370 406 604 51
Cuts at: 2894 2940 3101 3155 3310 3319 3331 3503 3569
Size: 46 161 54 155 9 12 172 66
Cuts at: 3569 3680 3714 3815 3955 4021 4632 4725 4799
Size: 111 34 101 140 66 611 93 74
Cuts at: 4799 4989 5051 5783 215
Size: 190 62 732 1441
Fragments arranged by size:
1441 732 611 604 406 370 190 186
180 172 161 155 155 150 142 140
119 111 106 101 93 74 69 68
66 66 62 54 51 50 46 34
15 12 9 8
BspEI T'CCGG_A
Cuts at: 3689 6229 3689
Size: 2540 4469
BspHI T'CATG_A
Cuts at: 539 2351 3548 6185 539
Size: 1812 1197 2637 1363
Fragments arranged by size:
2637 1812 1363 1197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 636 967 1283 2372 3257 4057 4271 4573 4954
Size: 331 316 1089 885 800 214 302 381
Cuts at: 4954 636
Size: 2691
Fragments arranged by size:
2691 1089 885 800 381 331 316 302
214
BsrBI CCG'CTC
Cuts at: 50 599 2241 3609 5299 5353 6839 7004 50
Size: 549 1642 1368 1690 54 1486 165 55
Fragments arranged by size:
1690 1642 1486 1368 549 165 55 54
BsrDI GCAATG_nn'
Cuts at: 279 294 437 4919 279
Size: 15 143 4482 2369
Fragments arranged by size:
4482 2369 143 15
BsrFI r'CCGG_y
Cuts at: 224 1607 5005 5186 5397 224
Size: 1383 3398 181 211 1836
Fragments arranged by size:
3398 1836 1383 211 181
BssSI C'ACGA_G
Cuts at: 5278 5638 5278
Size: 360 6649
BstAPI GCAn_nnn'nTGC
Cuts at: 4384 4384
Size: 7009
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7009
BstEII G'GTnAC_C
Cuts at: 2868 3763 2868
Size: 895 6114
BstXI CCAn_nnnn'nTGG
Cuts at: 1696 3760 1696
Size: 2064 4945
BstYI r'GATC_y
Cuts at: 1 1047 1097 1601 1769 2163 3507 3756 4032
Size: 1046 50 504 168 394 1344 249 276
Cuts at: 4032 4499 4522 4857 5103 6117 6203 6215 6561
Size: 467 23 335 246 1014 86 12 346
Cuts at: 6561 1
Size: 449
Fragments arranged by size:
1344 1046 1014 504 467 449 394 346
335 276 249 246 168 86 50 23
12
Bsu36I CC'TnA_GG
Cuts at: 348 988 1322 1961 2029 348
Size: 640 334 639 68 5328
Fragments arranged by size:
5328 640 639 334 68
BtgI C'CryG_G
Cuts at: 707 1433 1643 2203 2595 3815 707
Size: 726 210 560 392 1220 3901
Fragments arranged by size:
3901 1220 726 560 392 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4957 5138 5301 4957
Size: 181 163 6665
Fragments arranged by size:
6665 181 163
BtsI GCAGTG_nn'
Cuts at: 507 682 932 2625 3150 3864 4250 507
Size: 175 250 1693 525 714 386 3266
Fragments arranged by size:
3266 1693 714 525 386 250 175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1934 1969 1934
Size: 35 6974
DraI TTT'AAA
Cuts at: 4006 4006
Size: 7009
DrdI GACnn_nn'nnGTC
Cuts at: 3544 4713 5573 3544
Size: 1169 860 4980
Fragments arranged by size:
4980 1169 860
EaeI y'GGCC_r
Cuts at: 248 611 2249 3527 4592 4766 5157 5184 6531
Size: 363 1638 1278 1065 174 391 27 1347
Cuts at: 6531 248
Size: 726
Fragments arranged by size:
1638 1347 1278 1065 726 391 363 174
27
EagI C'GGCC_G
Cuts at: 4592 4592
Size: 7009
EarI CTCTTCn'nnn_
Cuts at: 1237 2489 2559 2822 3438 3787 3815 5030 5240
Size: 1252 70 263 616 349 28 1215 210
Cuts at: 5240 1237
Size: 3006
Fragments arranged by size:
3006 1252 1215 616 349 263 210 70
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1063 2156 2275 3214 5215 5527 5673 6819 1063
Size: 1093 119 939 2001 312 146 1146 1253
Fragments arranged by size:
2001 1253 1146 1093 939 312 146 119
EcoICRI GAG'CTC
Cuts at: 332 558 894 3099 3953 4019 332
Size: 226 336 2205 854 66 3322
Fragments arranged by size:
3322 2205 854 336 226 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 706 751 760 1488 1689 1936 1971 2059
Size: 309 45 9 728 201 247 35 88
Cuts at: 2059 2280 2696 2926 2975 3055 3112 3225 3487
Size: 221 416 230 49 80 57 113 262
Cuts at: 3487 3555 3613 3829 4832 5264 6013 6281 397
Size: 68 58 216 1003 432 749 268 1125
Fragments arranged by size:
1125 1003 749 728 432 416 309 268
262 247 230 221 216 201 113 88
80 68 58 57 49 45 35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 233 688 1234 1279 1525 1862 2801 2954 3977
Size: 455 546 45 246 337 939 153 1023
Cuts at: 3977 233
Size: 3265
Fragments arranged by size:
3265 1023 939 546 455 337 246 153
45
EcoO109I rG'GnC_Cy
Cuts at: 162 212 238 605 968 1036 1054 1115 1116
Size: 50 26 367 363 68 18 61 1
Cuts at: 1116 1284 1295 1296 1681 1700 1735 1884 1941
Size: 168 11 1 385 19 35 149 57
Cuts at: 1941 2361 2806 2959 3133 3152 4146 162
Size: 420 445 153 174 19 994 3025
Fragments arranged by size:
3025 994 445 420 385 367 363 174
168 153 149 68 61 57 50 35
26 19 19 18 11 1 1
EcoRI G'AATT_C
Cuts at: 4011 4011
Size: 7009
EcoRV GAT'ATC
Cuts at: 1581 1581
Size: 7009
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1032 1500 5026 1032
Size: 468 3526 3015
Fragments arranged by size:
3526 3015 468
FspI TGC'GCA
Cuts at: 4788 4788
Size: 7009
HaeII r_GCGC'y
Cuts at: 585 1667 2182 2649 2683 4441 4689 5713 6692
Size: 1082 515 467 34 1758 248 1024 979
Cuts at: 6692 585
Size: 902
Fragments arranged by size:
1758 1082 1024 979 902 515 467 248
34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 466 498 769 801 2121 2153 2155 2187 3265
Size: 32 271 32 1320 32 2 32 1078
Cuts at: 3265 3297 3568 3600 4849 4879 4881 4911 466
Size: 32 271 32 1249 30 2 30 2564
Fragments arranged by size:
2564 1320 1249 1078 271 271 32 32
32 32 32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 4046 4046
Size: 7009
HindIII A'AGCT_T
Cuts at: 562 562
Size: 7009
KasI G'GCGC_C
Cuts at: 1663 4437 4685 1663
Size: 2774 248 3987
Fragments arranged by size:
3987 2774 248
KpnI G_GTAC'C
Cuts at: 881 1065 2037 4027 881
Size: 184 972 1990 3863
Fragments arranged by size:
3863 1990 972 184
MluI A'CGCG_T
Cuts at: 6442 6442
Size: 7009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 320 2321 5680 5864 320
Size: 2001 3359 184 1465
Fragments arranged by size:
3359 2001 1465 184
MscI TGG'CCA
Cuts at: 613 2251 3529 4768 613
Size: 1638 1278 1239 2854
Fragments arranged by size:
2854 1638 1278 1239
MslI CAynn'nnrTG
Cuts at: 712 1128 2350 2353 2836 3770 4996 5123 712
Size: 416 1222 3 483 934 1226 127 2598
Fragments arranged by size:
2598 1226 1222 934 483 416 127 3
MspA1I CmG'CkG
Cuts at: 327 1226 1445 1991 2761 2848 3090 3657 3848
Size: 899 219 546 770 87 242 567 191
Cuts at: 3848 4118 4432 4792 5807 6052 327
Size: 270 314 360 1015 245 1284
Fragments arranged by size:
1284 1015 899 770 567 546 360 314
270 245 242 219 191 87
NaeI GCC'GGC
Cuts at: 5188 5188
Size: 7009
NarI GG'CG_CC
Cuts at: 1664 4438 4686 1664
Size: 2774 248 3987
Fragments arranged by size:
3987 2774 248
NcoI C'CATG_G
Cuts at: 707 1433 3815 707
Size: 726 2382 3901
Fragments arranged by size:
3901 2382 726
NgoMIV G'CCGG_C
Cuts at: 5186 5186
Size: 7009
NruI TCG'CGA
Cuts at: 5370 5370
Size: 7009
NspI r_CATG'y
Cuts at: 717 4060 4560 5469 717
Size: 3343 500 909 2257
Fragments arranged by size:
3343 2257 909 500
PasI CC'CwG_GG
Cuts at: 208 1696 3136 208
Size: 1488 1440 4081
Fragments arranged by size:
4081 1488 1440
PciI A'CATG_T
Cuts at: 713 5465 713
Size: 4752 2257
PflMI CCAn_nnn'nTGG
Cuts at: 208 912 1242 2726 208
Size: 704 330 1484 4491
Fragments arranged by size:
4491 1484 704 330
PfoI T'CCnGG_A
Cuts at: 1235 1583 3503 1235
Size: 348 1920 4741
Fragments arranged by size:
4741 1920 348
PmeI GTTT'AAAC
Cuts at: 4006 4006
Size: 7009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1519 1551 6148 6180 1519
Size: 32 4597 32 2348
Fragments arranged by size:
4597 2348 32 32
PpuMI rG'GwC_Cy
Cuts at: 162 605 1036 1284 1700 2361 162
Size: 443 431 248 416 661 4810
Fragments arranged by size:
4810 661 443 431 416 248
PspOMI G'GGCC_C
Cuts at: 211 1115 1295 1829 3151 211
Size: 904 180 534 1322 4069
Fragments arranged by size:
4069 1322 904 534 180
PspXI vC'TCGA_Gb
Cuts at: 2798 2798
Size: 7009
PstI C_TGCA'G
Cuts at: 499 623 1367 1622 3089 4054 4739 499
Size: 124 744 255 1467 965 685 2769
Fragments arranged by size:
2769 1467 965 744 685 255 124
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7009
PvuII CAG'CTG
Cuts at: 1226 1445 1991 2848 3090 4432 4792 1226
Size: 219 546 857 242 1342 360 3443
Fragments arranged by size:
3443 1342 857 546 360 242 219
RsrII CG'GwC_CG
Cuts at: 5202 5202
Size: 7009
SacI G_AGCT'C
Cuts at: 334 560 896 3101 3955 4021 334
Size: 226 336 2205 854 66 3322
Fragments arranged by size:
3322 2205 854 336 226 66
SalI G'TCGA_C
Cuts at: 4044 4044
Size: 7009
SanDI GG'GwC_CC
Cuts at: 162 605 162
Size: 443 6566
SapI GCTCTTCn'nnn_
Cuts at: 2489 2559 5030 5240 2489
Size: 70 2471 210 4258
Fragments arranged by size:
4258 2471 210 70
SbfI CC_TGCA'GG
Cuts at: 4054 4054
Size: 7009
ScaI AGT'ACT
Cuts at: 6526 6526
Size: 7009
SexAI A'CCwGG_T
Cuts at: 3482 3560 3482
Size: 78 6931
SfcI C'TryA_G
Cuts at: 33 444 495 619 1363 1618 1862 3085 3974
Size: 411 51 124 744 255 244 1223 889
Cuts at: 3974 4050 4735 5730 5921 33
Size: 76 685 995 191 1121
Fragments arranged by size:
1223 1121 995 889 744 685 411 255
244 191 124 76 51
SfoI GGC'GCC
Cuts at: 1665 4439 4687 1665
Size: 2774 248 3987
Fragments arranged by size:
3987 2774 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7009
SmaI CCC'GGG
Cuts at: 517 4029 517
Size: 3512 3497
SmlI C'TyrA_G
Cuts at: 559 2798 3051 4162 5077 5571 5833 559
Size: 2239 253 1111 915 494 262 1735
Fragments arranged by size:
2239 1735 1111 915 494 262 253
SnaBI TAC'GTA
Cuts at: 3999 3999
Size: 7009
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7009
SphI G_CATG'C
Cuts at: 4060 4560 4060
Size: 500 6509
SrfI GCCC'GGGC
Cuts at: 517 517
Size: 7009
StuI AGG'CCT
Cuts at: 171 986 171
Size: 815 6194
StyI C'CwwG_G
Cuts at: 707 1433 1880 1952 2462 2574 2885 3311 3815
Size: 726 447 72 510 112 311 426 504
Cuts at: 3815 3888 3899 4141 4495 707
Size: 73 11 242 354 3221
Fragments arranged by size:
3221 726 510 504 447 426 354 311
242 112 73 72 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 441 1236 1343 3658 3766 4590 4723 5182 5393
Size: 795 107 2315 108 824 133 459 211
Cuts at: 5393 441
Size: 2057
Fragments arranged by size:
2315 2057 824 795 459 211 133 108
107
TatI w'GTAC_w
Cuts at: 758 6524 758
Size: 5766 1243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 2617 2649 3741 3773 6526 6558 328
Size: 32 2257 32 1092 32 2753 32 779
Fragments arranged by size:
2753 2257 1092 779 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4804 4804
Size: 7009
XbaI T'CTAG_A
Cuts at: 310 4038 310
Size: 3728 3281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 726 2313 4502 726
Size: 1587 2189 3233
Fragments arranged by size:
3233 2189 1587
XhoI C'TCGA_G
Cuts at: 2798 3051 2798
Size: 253 6756
XmaI C'CCGG_G
Cuts at: 515 4027 515
Size: 3512 3497
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AloI AscI AvrII BaeI BfrBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I ClaI DraIII
FseI FspAI HpaI MfeI NdeI NheI NotI NsiI
PacI PmlI PshAI PsiI PsrI SacII SfiI SgrAI
SspI SwaI XmnI ZraI