Restriction map for
pF1KSDA0436
(Circular) (Six-base) MAPSORT of: pF1KSDA0436.seq Check: 6341 from: 1 to: 5053
pF1KSDA0436 5053 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2089 2089
Size: 5053
Acc65I G'GTAC_C
Cuts at: 1800 2067 1800
Size: 267 4786
AclI AA'CG_TT
Cuts at: 1722 4893 1722
Size: 3171 1882
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 416 704 967 2876 3308 4057 4325 407
Size: 9 288 263 1909 432 749 268 1135
Fragments arranged by size:
1909 1135 749 432 288 268 263 9
AflII C'TTAA_G
Cuts at: 310 2020 310
Size: 1710 3343
AflIII A'CryG_T
Cuts at: 917 1287 2403 3509 4486 917
Size: 370 1116 1106 977 1484
Fragments arranged by size:
1484 1116 1106 977 370
AgeI A'CCGG_T
Cuts at: 3441 3441
Size: 5053
AlwNI CAG_nnn'CTG
Cuts at: 844 2356 3925 844
Size: 1512 1569 1972
Fragments arranged by size:
1972 1569 1512
ApaLI G'TGCA_C
Cuts at: 3823 3823
Size: 5053
ApoI r'AATT_y
Cuts at: 820 1050 1323 1969 2035 2055 4406 4662 820
Size: 230 273 646 66 20 2351 256 1211
Fragments arranged by size:
2351 1211 646 273 256 230 66 20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5053
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5053
AvaI C'yCGr_G
Cuts at: 2071 2071
Size: 5053
BamHI G'GATC_C
Cuts at: 2076 2076
Size: 5053
BanI G'GyrC_C
Cuts at: 1605 1800 2067 2481 2729 2764 1605
Size: 195 267 414 248 35 3894
Fragments arranged by size:
3894 414 267 248 195 35
BanII G_rGCy'C
Cuts at: 712 2065 3095 712
Size: 1353 1030 2670
Fragments arranged by size:
2670 1353 1030
BbeI G_GCGC'C
Cuts at: 2485 2733 2485
Size: 248 4805
BbsI GAAGACnn'nnnn_
Cuts at: 1166 1166
Size: 5053
BbvCI CC'TCA_GC
Cuts at: 1751 1751
Size: 5053
BciVI GTATCCnnnnn_n'
Cuts at: 1038 2949 3712 5053 1038
Size: 1911 763 1341 1038
Fragments arranged by size:
1911 1341 1038 763
BclI T'GATC_A
Cuts at: 2571 2571
Size: 5053
BfrBI ATG'CAT
Cuts at: 133 133
Size: 5053
BglI GCCn_nnn'nGGC
Cuts at: 1338 1338
Size: 5053
BglII A'GATC_T
Cuts at: 2566 4605 2566
Size: 2039 3014
BlpI GC'TnA_GC
Cuts at: 424 2163 424
Size: 1739 3314
Bme1580I G_kGCm'C
Cuts at: 2676 2769 3827 2676
Size: 93 1058 3902
Fragments arranged by size:
3902 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1160 2263 2344 2679 4825 1160
Size: 1103 81 335 2146 1388
Fragments arranged by size:
2146 1388 1103 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 3418
Size: 5053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 1393 1559 1937 335
Size: 1058 166 378 3451
Fragments arranged by size:
3451 1058 378 166
Bpu10I CC'TnA_GC
Cuts at: 706 1751 1862 706
Size: 1045 111 3897
Fragments arranged by size:
3897 1045 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2227 3106 3600 3898 1822
Size: 405 879 494 298 2977
Fragments arranged by size:
2977 879 494 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 1010 4757 1010
Size: 3747 1306
BsaAI yAC'GTr
Cuts at: 2043 2404 2043
Size: 361 4692
BsaBI GATnn'nnATC
Cuts at: 6 2565 4604 6
Size: 2559 2039 455
Fragments arranged by size:
2559 2039 455
BsaHI Gr'CG_yC
Cuts at: 2482 2730 3434 4930 2482
Size: 248 704 1496 2605
Fragments arranged by size:
2605 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2240 2761 3441 3715 3862 4273 2240
Size: 521 680 274 147 411 3020
Fragments arranged by size:
3020 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2476 3234 3813 4309 2476
Size: 758 579 496 3220
Fragments arranged by size:
3220 758 579 496
BsiEI CG_ry'CG
Cuts at: 104 2593 2639 3849 104
Size: 2489 46 1210 1308
Fragments arranged by size:
2489 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2065 2843 3033 3827 2065
Size: 778 190 794 3291
Fragments arranged by size:
3291 794 778 190
Bsp1286I G_dGCh'C
Cuts at: 712 2065 2676 2769 2843 3033 3095 3827 712
Size: 1353 611 93 74 190 62 732 1938
Fragments arranged by size:
1938 1353 732 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4273 4273
Size: 5053
BspHI T'CATG_A
Cuts at: 540 4229 540
Size: 3689 1364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2101 2315 2617 2998 2101
Size: 214 302 381 4156
Fragments arranged by size:
4156 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1591 3343 3397 4883 5048 50
Size: 1541 1752 54 1486 165 55
Fragments arranged by size:
1752 1541 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2963 2963
Size: 5053
BsrFI r'CCGG_y
Cuts at: 3049 3230 3441 3049
Size: 181 211 4661
Fragments arranged by size:
4661 211 181
BssSI C'ACGA_G
Cuts at: 3322 3682 3322
Size: 360 4693
BstAPI GCAn_nnn'nTGC
Cuts at: 2428 2428
Size: 5053
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5053
BstYI r'GATC_y
Cuts at: 1 2016 2076 2543 2566 2901 3147 4161 4247
Size: 2015 60 467 23 335 246 1014 86
Cuts at: 4247 4259 4605 1
Size: 12 346 449
Fragments arranged by size:
2015 1014 467 449 346 335 246 86
60 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 5053
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1833 3001 3182 3345 1833
Size: 1168 181 163 3541
Fragments arranged by size:
3541 1168 181 163
BtsI GCAGTG_nn'
Cuts at: 2294 2294
Size: 5053
DraI TTT'AAA
Cuts at: 2050 2050
Size: 5053
DrdI GACnn_nn'nnGTC
Cuts at: 2757 3617 2757
Size: 860 4193
EaeI y'GGCC_r
Cuts at: 1425 1488 2636 2810 3201 3228 4575 1425
Size: 63 1148 174 391 27 1347 1903
Fragments arranged by size:
1903 1347 1148 391 174 63 27
EagI C'GGCC_G
Cuts at: 2636 2636
Size: 5053
EarI CTCTTCn'nnn_
Cuts at: 883 3074 3284 883
Size: 2191 210 2652
Fragments arranged by size:
2652 2191 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3259 3571 3717 4863 3259
Size: 312 146 1146 3449
Fragments arranged by size:
3449 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2063 2063
Size: 5053
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 407 416 704 967 1393 1559 1937 2876
Size: 72 9 288 263 426 166 378 939
Cuts at: 2876 3308 4057 4325 335
Size: 432 749 268 1063
Fragments arranged by size:
1063 939 749 432 426 378 288 268
263 166 72 9
EcoNI CCTnn'n_nnAGG
Cuts at: 522 1808 1978 522
Size: 1286 170 3597
Fragments arranged by size:
3597 1286 170
EcoO109I rG'GnC_Cy
Cuts at: 717 753 1344 2190 717
Size: 36 591 846 3580
Fragments arranged by size:
3580 846 591 36
EcoRI G'AATT_C
Cuts at: 2055 2055
Size: 5053
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3070 3070
Size: 5053
FspI TGC'GCA
Cuts at: 2832 2832
Size: 5053
HaeII r_GCGC'y
Cuts at: 2485 2733 3757 4736 2485
Size: 248 1024 979 2802
Fragments arranged by size:
2802 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2893 2923 2925 2955 2893
Size: 30 2 30 4991
Fragments arranged by size:
4991 30 30 2
HincII GTy'rAC
Cuts at: 2090 2090
Size: 5053
HindIII A'AGCT_T
Cuts at: 446 446
Size: 5053
KasI G'GCGC_C
Cuts at: 2481 2729 2481
Size: 248 4805
KpnI G_GTAC'C
Cuts at: 1804 2071 1804
Size: 267 4786
MluI A'CGCG_T
Cuts at: 4486 4486
Size: 5053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 786 3724 3908 786
Size: 2938 184 1931
Fragments arranged by size:
2938 1931 184
MscI TGG'CCA
Cuts at: 1490 2812 1490
Size: 1322 3731
MslI CAynn'nnrTG
Cuts at: 193 776 1292 1421 1633 3040 3167 193
Size: 583 516 129 212 1407 127 2079
Fragments arranged by size:
2079 1407 583 516 212 129 127
MspA1I CmG'CkG
Cuts at: 841 2162 2476 2836 3851 4096 841
Size: 1321 314 360 1015 245 1798
Fragments arranged by size:
1798 1321 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3232 3232
Size: 5053
NarI GG'CG_CC
Cuts at: 2482 2730 2482
Size: 248 4805
NcoI C'CATG_G
Cuts at: 125 125
Size: 5053
NdeI CA'TA_TG
Cuts at: 1782 1782
Size: 5053
NgoMIV G'CCGG_C
Cuts at: 3230 3230
Size: 5053
NruI TCG'CGA
Cuts at: 3414 3414
Size: 5053
NsiI A_TGCA'T
Cuts at: 135 135
Size: 5053
NspI r_CATG'y
Cuts at: 921 1291 1861 2104 2604 3513 921
Size: 370 570 243 500 909 2461
Fragments arranged by size:
2461 909 570 500 370 243
PciI A'CATG_T
Cuts at: 917 1287 3509 917
Size: 370 2222 2461
Fragments arranged by size:
2461 2222 370
PmeI GTTT'AAAC
Cuts at: 2050 2050
Size: 5053
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4192 4224 4192
Size: 32 5021
PpuMI rG'GwC_Cy
Cuts at: 717 753 1344 717
Size: 36 591 4426
Fragments arranged by size:
4426 591 36
PsiI TTA'TAA
Cuts at: 418 418
Size: 5053
PstI C_TGCA'G
Cuts at: 2098 2783 2098
Size: 685 4368
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5053
PvuII CAG'CTG
Cuts at: 2476 2836 2476
Size: 360 4693
RsrII CG'GwC_CG
Cuts at: 3246 3246
Size: 5053
SacI G_AGCT'C
Cuts at: 2065 2065
Size: 5053
SalI G'TCGA_C
Cuts at: 2088 2088
Size: 5053
SanDI GG'GwC_CC
Cuts at: 717 717
Size: 5053
SapI GCTCTTCn'nnn_
Cuts at: 3074 3284 3074
Size: 210 4843
SbfI CC_TGCA'GG
Cuts at: 2098 2098
Size: 5053
ScaI AGT'ACT
Cuts at: 728 4570 728
Size: 3842 1211
SfcI C'TryA_G
Cuts at: 33 815 2094 2779 3774 3965 33
Size: 782 1279 685 995 191 1121
Fragments arranged by size:
1279 1121 995 782 685 191
SfoI GGC'GCC
Cuts at: 2483 2731 2483
Size: 248 4805
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5053
SmaI CCC'GGG
Cuts at: 2073 2073
Size: 5053
SmlI C'TyrA_G
Cuts at: 310 1837 2020 2206 3121 3615 3877 310
Size: 1527 183 186 915 494 262 1486
Fragments arranged by size:
1527 1486 915 494 262 186 183
SnaBI TAC'GTA
Cuts at: 2043 2043
Size: 5053
SpeI A'CTAG_T
Cuts at: 67 1177 67
Size: 1110 3943
SphI G_CATG'C
Cuts at: 1861 2104 2604 1861
Size: 243 500 4310
Fragments arranged by size:
4310 500 243
SspI AAT'ATT
Cuts at: 663 1108 1743 1896 663
Size: 445 635 153 3820
Fragments arranged by size:
3820 635 445 153
StuI AGG'CCT
Cuts at: 1332 1406 1332
Size: 74 4979
StyI C'CwwG_G
Cuts at: 125 712 1021 1491 2185 2539 125
Size: 587 309 470 694 354 2639
Fragments arranged by size:
2639 694 587 470 354 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 992 2634 2767 3226 3437 992
Size: 1642 133 459 211 2608
Fragments arranged by size:
2608 1642 459 211 133
TatI w'GTAC_w
Cuts at: 726 4568 726
Size: 3842 1211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1208 1240 1632 1664 4570 4602 1208
Size: 32 392 32 2906 32 1659
Fragments arranged by size:
2906 1659 392 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2848 2848
Size: 5053
XbaI T'CTAG_A
Cuts at: 1194 1902 2082 1194
Size: 708 180 4165
Fragments arranged by size:
4165 708 180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1387 2546 1387
Size: 1159 3894
XmaI C'CCGG_G
Cuts at: 2071 2071
Size: 5053
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BmgBI BmtI BsaXI BseRI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NheI NotI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SexAI SfiI
SgrAI SrfI SwaI XhoI XmnI ZraI