Restriction map for
pF1KSDA0420
(Circular) (Six-base) MAPSORT of: pF1KSDA0420.seq Check: 3132 from: 1 to: 5227
pF1KSDA0420 5227 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 334 334
Size: 5227
AccI GT'mk_AC
Cuts at: 158 1955 2263 158
Size: 1797 308 3122
Fragments arranged by size:
3122 1797 308
Acc65I G'GTAC_C
Cuts at: 646 2241 646
Size: 1595 3632
AclI AA'CG_TT
Cuts at: 5067 5067
Size: 5227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 2085 3050 3482 4231 4499 1012
Size: 1073 965 432 749 268 1740
Fragments arranged by size:
1740 1073 965 749 432 268
AflIII A'CryG_T
Cuts at: 2577 3683 4660 2577
Size: 1106 977 3144
Fragments arranged by size:
3144 1106 977
AgeI A'CCGG_T
Cuts at: 3615 3615
Size: 5227
AhdI GACnn_n'nnGTC
Cuts at: 528 1373 528
Size: 845 4382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1061 1061
Size: 5227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1753 1785 1753
Size: 32 5195
AlwNI CAG_nnn'CTG
Cuts at: 1259 2530 4099 1259
Size: 1271 1569 2387
Fragments arranged by size:
2387 1569 1271
ApaI G_GGCC'C
Cuts at: 749 767 2054 749
Size: 18 1287 3922
Fragments arranged by size:
3922 1287 18
ApaLI G'TGCA_C
Cuts at: 280 3997 280
Size: 3717 1510
ApoI r'AATT_y
Cuts at: 2229 4580 4836 2229
Size: 2351 256 2620
Fragments arranged by size:
2620 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5227
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5227
AvaI C'yCGr_G
Cuts at: 968 1400 1811 2010 2047 2245 968
Size: 432 411 199 37 198 3950
Fragments arranged by size:
3950 432 411 199 198 37
BamHI G'GATC_C
Cuts at: 2250 2250
Size: 5227
BanI G'GyrC_C
Cuts at: 646 824 1298 1794 1807 2241 2655 2903 2938
Size: 178 474 496 13 434 414 248 35
Cuts at: 2938 646
Size: 2935
Fragments arranged by size:
2935 496 474 434 414 248 178 35
13
BanII G_rGCy'C
Cuts at: 635 749 767 945 1398 1689 1930 2054 2110
Size: 114 18 178 453 291 241 124 56
Cuts at: 2110 2239 3269 635
Size: 129 1030 2593
Fragments arranged by size:
2593 1030 453 291 241 178 129 124
114 56 18
BbeI G_GCGC'C
Cuts at: 1798 1811 2659 2907 1798
Size: 13 848 248 4118
Fragments arranged by size:
4118 848 248 13
BbsI GAAGACnn'nnnn_
Cuts at: 222 354 494 1398 222
Size: 132 140 904 4051
Fragments arranged by size:
4051 904 140 132
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 527 910 944 493
Size: 34 383 34 4776
Fragments arranged by size:
4776 383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3123 3886 5227 3123
Size: 763 1341 3123
Fragments arranged by size:
3123 1341 763
BclI T'GATC_A
Cuts at: 1427 2745 1427
Size: 1318 3909
BglII A'GATC_T
Cuts at: 2740 4779 2740
Size: 2039 3188
BlpI GC'TnA_GC
Cuts at: 2152 2337 2152
Size: 185 5042
Bme1580I G_kGCm'C
Cuts at: 217 284 749 767 2054 2850 2943 4001 217
Size: 67 465 18 1287 796 93 1058 1443
Fragments arranged by size:
1443 1287 1058 796 465 93 67 18
BmgBI CAC'GTC
Cuts at: 1764 1764
Size: 5227
BmrI ACTGGGnnnn_n'
Cuts at: 220 673 2437 2518 2853 4999 220
Size: 453 1764 81 335 2146 448
Fragments arranged by size:
2146 1764 453 448 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 654 3592 654
Size: 2938 2289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 793 1075 1300 1948 2193 754
Size: 39 282 225 648 245 3788
Fragments arranged by size:
3788 648 282 245 225 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1011 2401 3280 3774 4072 1011
Size: 1390 879 494 298 2166
Fragments arranged by size:
2166 1390 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 883 1360 1648 2211 4931 418
Size: 465 477 288 563 2720 714
Fragments arranged by size:
2720 714 563 477 465 288
BsaAI yAC'GTr
Cuts at: 212 285 357 2217 2578 212
Size: 73 72 1860 361 2861
Fragments arranged by size:
2861 1860 361 73 72
BsaBI GATnn'nnATC
Cuts at: 6 2739 4778 6
Size: 2733 2039 455
Fragments arranged by size:
2733 2039 455
BsaHI Gr'CG_yC
Cuts at: 317 670 1795 1808 1952 2656 2904 3608 5104
Size: 353 1125 13 144 704 248 704 1496
Cuts at: 5104 317
Size: 440
Fragments arranged by size:
1496 1125 704 704 440 353 248 144
13
BsaWI w'CCGG_w
Cuts at: 2414 2935 3615 3889 4036 4447 2414
Size: 521 680 274 147 411 3194
Fragments arranged by size:
3194 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 712 742 1493 1523 1544 1571 1574 1601 1712
Size: 30 751 30 21 27 3 27 111
Cuts at: 1712 1742 712
Size: 30 4197
Fragments arranged by size:
4197 751 111 30 30 30 27 27
21 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 394 703 1012 1072 1180 1630 2163 2166 394
Size: 309 309 60 108 450 533 3 3455
Fragments arranged by size:
3455 533 450 309 309 108 60 3
BseYI C'CCAG_C
Cuts at: 724 1239 1803 1856 1987 2147 2650 3408 3987
Size: 515 564 53 131 160 503 758 579
Cuts at: 3987 4483 724
Size: 496 1468
Fragments arranged by size:
1468 758 579 564 515 503 496 160
131 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 2023 2115 388
Size: 1635 92 3500
Fragments arranged by size:
3500 1635 92
BsiEI CG_ry'CG
Cuts at: 104 1312 2767 2813 4023 104
Size: 1208 1455 46 1210 1308
Fragments arranged by size:
1455 1308 1210 1208 46
BsiHKAI G_wGCw'C
Cuts at: 284 356 458 635 923 1418 1628 1679 2087
Size: 72 102 177 288 495 210 51 408
Cuts at: 2087 2239 3017 3207 4001 284
Size: 152 778 190 794 1510
Fragments arranged by size:
1510 794 778 495 408 288 210 190
177 152 102 72 51
Bsp1286I G_dGCh'C
Cuts at: 217 284 356 458 635 749 767 923 945
Size: 67 72 102 177 114 18 156 22
Cuts at: 945 1398 1418 1628 1679 1689 1930 2054 2087
Size: 453 20 210 51 10 241 124 33
Cuts at: 2087 2110 2239 2850 2943 3017 3207 3269 4001
Size: 23 129 611 93 74 190 62 732
Cuts at: 4001 217
Size: 1443
Fragments arranged by size:
1443 732 611 453 241 210 190 177
156 129 124 114 102 93 74 72
67 62 51 33 23 22 20 18
10
BspEI T'CCGG_A
Cuts at: 4447 4447
Size: 5227
BspHI T'CATG_A
Cuts at: 945 4403 945
Size: 3458 1769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 334 1273 1967 2275 2489 2791 3172 334
Size: 939 694 308 214 302 381 2389
Fragments arranged by size:
2389 939 694 381 308 302 214
BsrBI CCG'CTC
Cuts at: 50 294 769 3517 3571 5057 5222 50
Size: 244 475 2748 54 1486 165 55
Fragments arranged by size:
2748 1486 475 244 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3137 3137
Size: 5227
BsrFI r'CCGG_y
Cuts at: 302 3223 3404 3615 302
Size: 2921 181 211 1914
Fragments arranged by size:
2921 1914 211 181
BssSI C'ACGA_G
Cuts at: 1693 3496 3856 1693
Size: 1803 360 3064
Fragments arranged by size:
3064 1803 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2602 2602
Size: 5227
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5227
BstXI CCAn_nnnn'nTGG
Cuts at: 441 732 1995 441
Size: 291 1263 3673
Fragments arranged by size:
3673 1263 291
BstYI r'GATC_y
Cuts at: 1 221 1017 1709 2250 2717 2740 3075 3321
Size: 220 796 692 541 467 23 335 246
Cuts at: 3321 4335 4421 4433 4779 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 796 692 541 467 449 346 335
246 220 86 23 12
BtgI C'CryG_G
Cuts at: 125 322 741 1157 1680 1703 125
Size: 197 419 416 523 23 3649
Fragments arranged by size:
3649 523 419 416 197 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3175 3356 3519 3175
Size: 181 163 4883
Fragments arranged by size:
4883 181 163
BtsI GCAGTG_nn'
Cuts at: 456 1651 2468 456
Size: 1195 817 3215
Fragments arranged by size:
3215 1195 817
DraI TTT'AAA
Cuts at: 2224 2224
Size: 5227
DraIII CAC_nnn'GTG
Cuts at: 288 1014 1698 288
Size: 726 684 3817
Fragments arranged by size:
3817 726 684
DrdI GACnn_nn'nnGTC
Cuts at: 2931 3791 2931
Size: 860 4367
EaeI y'GGCC_r
Cuts at: 1098 1309 1700 2181 2810 2984 3375 3402 4749
Size: 211 391 481 629 174 391 27 1347
Cuts at: 4749 1098
Size: 1576
Fragments arranged by size:
1576 1347 629 481 391 391 211 174
27
EagI C'GGCC_G
Cuts at: 1309 2810 1309
Size: 1501 3726
EarI CTCTTCn'nnn_
Cuts at: 1607 3248 3458 1607
Size: 1641 210 3376
Fragments arranged by size:
3376 1641 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1108 1473 3433 3745 3891 5037 1108
Size: 365 1960 312 146 1146 1298
Fragments arranged by size:
1960 1298 1146 365 312 146
EcoICRI GAG'CTC
Cuts at: 633 2237 633
Size: 1604 3623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 793 1012 1075 1300 1948 2085 2193 3050
Size: 39 219 63 225 648 137 108 857
Cuts at: 3050 3482 4231 4499 754
Size: 432 749 268 1482
Fragments arranged by size:
1482 857 749 648 432 268 225 219
137 108 63 39
EcoNI CCTnn'n_nnAGG
Cuts at: 961 1054 961
Size: 93 5134
EcoO109I rG'GnC_Cy
Cuts at: 720 746 763 1323 1495 1860 2364 720
Size: 26 17 560 172 365 504 3583
Fragments arranged by size:
3583 560 504 365 172 26 17
EcoRI G'AATT_C
Cuts at: 2229 2229
Size: 5227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 3244
Size: 5227
FspI TGC'GCA
Cuts at: 3006 3006
Size: 5227
HaeII r_GCGC'y
Cuts at: 1174 1798 1811 2159 2659 2907 3931 4910 1174
Size: 624 13 348 500 248 1024 979 1491
Fragments arranged by size:
1491 1024 979 624 500 348 248 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 514 546 712 744 1359 1391 1517 1549 1571
Size: 32 166 32 615 32 126 32 22
Cuts at: 1571 1603 1712 1744 3067 3097 3099 3129 514
Size: 32 109 32 1323 30 2 30 2612
Fragments arranged by size:
2612 1323 615 166 126 109 32 32
32 32 32 32 30 30 22 2
HincII GTy'rAC
Cuts at: 1197 2264 1197
Size: 1067 4160
KasI G'GCGC_C
Cuts at: 1794 1807 2655 2903 1794
Size: 13 848 248 4118
Fragments arranged by size:
4118 848 248 13
KpnI G_GTAC'C
Cuts at: 650 2245 650
Size: 1595 3632
MluI A'CGCG_T
Cuts at: 4660 4660
Size: 5227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3898 4082 3898
Size: 184 5043
MscI TGG'CCA
Cuts at: 2986 2986
Size: 5227
MslI CAynn'nnrTG
Cuts at: 844 3214 3341 844
Size: 2370 127 2730
Fragments arranged by size:
2730 2370 127
MspA1I CmG'CkG
Cuts at: 324 1239 1638 1682 1835 1842 2138 2336 2650
Size: 915 399 44 153 7 296 198 314
Cuts at: 2650 3010 4025 4270 324
Size: 360 1015 245 1281
Fragments arranged by size:
1281 1015 915 399 360 314 296 245
198 153 44 7
NaeI GCC'GGC
Cuts at: 304 3406 304
Size: 3102 2125
NarI GG'CG_CC
Cuts at: 1795 1808 2656 2904 1795
Size: 13 848 248 4118
Fragments arranged by size:
4118 848 248 13
NcoI C'CATG_G
Cuts at: 125 125
Size: 5227
NgoMIV G'CCGG_C
Cuts at: 302 3404 302
Size: 3102 2125
NruI TCG'CGA
Cuts at: 3588 3588
Size: 5227
NspI r_CATG'y
Cuts at: 1186 1296 2278 2778 3687 1186
Size: 110 982 500 909 2726
Fragments arranged by size:
2726 982 909 500 110
PasI CC'CwG_GG
Cuts at: 642 759 1371 642
Size: 117 612 4498
Fragments arranged by size:
4498 612 117
PciI A'CATG_T
Cuts at: 3683 3683
Size: 5227
PflMI CCAn_nnn'nTGG
Cuts at: 1698 1698
Size: 5227
PfoI T'CCnGG_A
Cuts at: 1258 1712 1258
Size: 454 4773
PmeI GTTT'AAAC
Cuts at: 2224 2224
Size: 5227
PmlI CAC'GTG
Cuts at: 212 285 357 212
Size: 73 72 5082
Fragments arranged by size:
5082 73 72
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4366 4398 4366
Size: 32 5195
PpuMI rG'GwC_Cy
Cuts at: 720 1495 1860 720
Size: 775 365 4087
Fragments arranged by size:
4087 775 365
PspOMI G'GGCC_C
Cuts at: 745 763 2050 745
Size: 18 1287 3922
Fragments arranged by size:
3922 1287 18
PstI C_TGCA'G
Cuts at: 463 2005 2272 2957 463
Size: 1542 267 685 2733
Fragments arranged by size:
2733 1542 685 267
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5227
PvuII CAG'CTG
Cuts at: 1239 1638 1842 2650 3010 1239
Size: 399 204 808 360 3456
Fragments arranged by size:
3456 808 399 360 204
RsrII CG'GwC_CG
Cuts at: 3420 3420
Size: 5227
SacI G_AGCT'C
Cuts at: 635 2239 635
Size: 1604 3623
SacII CC_GC'GG
Cuts at: 325 1683 325
Size: 1358 3869
SalI G'TCGA_C
Cuts at: 2262 2262
Size: 5227
SanDI GG'GwC_CC
Cuts at: 720 1495 720
Size: 775 4452
SapI GCTCTTCn'nnn_
Cuts at: 3248 3458 3248
Size: 210 5017
SbfI CC_TGCA'GG
Cuts at: 2272 2272
Size: 5227
ScaI AGT'ACT
Cuts at: 4744 4744
Size: 5227
SexAI A'CCwGG_T
Cuts at: 1010 1010
Size: 5227
SfcI C'TryA_G
Cuts at: 33 459 1473 2001 2268 2953 3948 4139 33
Size: 426 1014 528 267 685 995 191 1121
Fragments arranged by size:
1121 1014 995 685 528 426 267 191
SfoI GGC'GCC
Cuts at: 1796 1809 2657 2905 1796
Size: 13 848 248 4118
Fragments arranged by size:
4118 848 248 13
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5227
SmaI CCC'GGG
Cuts at: 970 1813 2012 2049 2247 970
Size: 843 199 37 198 3950
Fragments arranged by size:
3950 843 199 198 37
SmlI C'TyrA_G
Cuts at: 990 2380 3295 3789 4051 990
Size: 1390 915 494 262 2166
Fragments arranged by size:
2166 1390 915 494 262
SnaBI TAC'GTA
Cuts at: 2217 2217
Size: 5227
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5227
SphI G_CATG'C
Cuts at: 2278 2778 2278
Size: 500 4727
StuI AGG'CCT
Cuts at: 1505 1505
Size: 5227
StyI C'CwwG_G
Cuts at: 125 554 1545 2359 2713 125
Size: 429 991 814 354 2639
Fragments arranged by size:
2639 991 814 429 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 518 729 1716 2808 2941 3400 3611 518
Size: 211 987 1092 133 459 211 2134
Fragments arranged by size:
2134 1092 987 459 211 211 133
TatI w'GTAC_w
Cuts at: 578 4742 578
Size: 4164 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1493 1525 1544 1576 1624 1656 4744 4776 1493
Size: 32 19 32 48 32 3088 32 1944
Fragments arranged by size:
3088 1944 48 32 32 32 32 19
Tth111I GACn'n_nGTC
Cuts at: 2023 3022 2023
Size: 999 4228
XbaI T'CTAG_A
Cuts at: 2256 2256
Size: 5227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 441 1093 2118 2720 441
Size: 652 1025 602 2948
Fragments arranged by size:
2948 1025 652 602
XmaI C'CCGG_G
Cuts at: 968 1811 2010 2047 2245 968
Size: 843 199 37 198 3950
Fragments arranged by size:
3950 843 199 198 37
XmnI GAAnn'nnTTC
Cuts at: 202 202
Size: 5227
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BaeI BbvCI
BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PshAI PsiI PspXI PsrI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI