Restriction map for
pF1KSDA0416
(Circular) (Six-base) MAPSORT of: pF1KSDA0416.seq Check: 3650 from: 1 to: 4687
pF1KSDA0416 4687 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1673 1723 1673
Size: 50 4637
Acc65I G'GTAC_C
Cuts at: 1701 1701
Size: 4687
AclI AA'CG_TT
Cuts at: 4527 4527
Size: 4687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 2510 2942 3691 3959 360
Size: 2150 432 749 268 1088
Fragments arranged by size:
2150 1088 749 432 268
AflII C'TTAA_G
Cuts at: 457 471 457
Size: 14 4673
AflIII A'CryG_T
Cuts at: 859 1556 2037 3143 4120 859
Size: 697 481 1106 977 1426
Fragments arranged by size:
1426 1106 977 697 481
AgeI A'CCGG_T
Cuts at: 3075 3075
Size: 4687
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1118 1150 1118
Size: 32 4655
AlwNI CAG_nnn'CTG
Cuts at: 1650 1990 3559 1650
Size: 340 1569 2778
Fragments arranged by size:
2778 1569 340
ApaI G_GGCC'C
Cuts at: 159 159
Size: 4687
ApaLI G'TGCA_C
Cuts at: 3457 3457
Size: 4687
ApoI r'AATT_y
Cuts at: 413 534 1209 1689 4040 4296 413
Size: 121 675 480 2351 256 804
Fragments arranged by size:
2351 804 675 480 256 121
AseI AT'TA_AT
Cuts at: 20 466 736 785 1619 20
Size: 446 270 49 834 3088
Fragments arranged by size:
3088 834 446 270 49
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4687
AvaI C'yCGr_G
Cuts at: 1705 1705
Size: 4687
BamHI G'GATC_C
Cuts at: 1710 1710
Size: 4687
BanI G'GyrC_C
Cuts at: 1701 2115 2363 2398 1701
Size: 414 248 35 3990
Fragments arranged by size:
3990 414 248 35
BanII G_rGCy'C
Cuts at: 159 313 353 581 1699 2729 159
Size: 154 40 228 1118 1030 2117
Fragments arranged by size:
2117 1118 1030 228 154 40
BbeI G_GCGC'C
Cuts at: 2119 2367 2119
Size: 248 4439
BciVI GTATCCnnnnn_n'
Cuts at: 2583 3346 4687 2583
Size: 763 1341 2583
Fragments arranged by size:
2583 1341 763
BclI T'GATC_A
Cuts at: 2205 2205
Size: 4687
BglII A'GATC_T
Cuts at: 1010 2200 4239 1010
Size: 1190 2039 1458
Fragments arranged by size:
2039 1458 1190
BlpI GC'TnA_GC
Cuts at: 808 1797 808
Size: 989 3698
Bme1580I G_kGCm'C
Cuts at: 159 886 2310 2403 3461 159
Size: 727 1424 93 1058 1385
Fragments arranged by size:
1424 1385 1058 727 93
BmrI ACTGGGnnnn_n'
Cuts at: 637 1897 1978 2313 4459 637
Size: 1260 81 335 2146 865
Fragments arranged by size:
2146 1260 865 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3052
Size: 4687
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 694 1440 694
Size: 746 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1861 2740 3234 3532 1861
Size: 879 494 298 3016
Fragments arranged by size:
3016 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1051 4391 1051
Size: 3340 1347
BsaAI yAC'GTr
Cuts at: 1677 2038 1677
Size: 361 4326
BsaBI GATnn'nnATC
Cuts at: 6 407 2199 4238 6
Size: 401 1792 2039 455
Fragments arranged by size:
2039 1792 455 401
BsaHI Gr'CG_yC
Cuts at: 2116 2364 3068 4564 2116
Size: 248 704 1496 2239
Fragments arranged by size:
2239 1496 704 248
BsaWI w'CCGG_w
Cuts at: 1874 2395 3075 3349 3496 3907 1874
Size: 521 680 274 147 411 2654
Fragments arranged by size:
2654 680 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1101 2110 2868 3447 3943 1101
Size: 1009 758 579 496 1845
Fragments arranged by size:
1845 1009 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 802 553
Size: 249 4438
BsiEI CG_ry'CG
Cuts at: 104 2227 2273 3483 104
Size: 2123 46 1210 1308
Fragments arranged by size:
2123 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 353 581 694 767 1089 1501 1699 2477 2667
Size: 228 113 73 322 412 198 778 190
Cuts at: 2667 3461 353
Size: 794 1579
Fragments arranged by size:
1579 794 778 412 322 228 198 190
113 73
BsmI GAATG_Cn'
Cuts at: 1072 1072
Size: 4687
Bsp1286I G_dGCh'C
Cuts at: 159 313 353 581 694 767 886 1089 1501
Size: 154 40 228 113 73 119 203 412
Cuts at: 1501 1699 2310 2403 2477 2667 2729 3461 159
Size: 198 611 93 74 190 62 732 1385
Fragments arranged by size:
1385 732 611 412 228 203 198 190
154 119 113 93 74 73 62 40
BspEI T'CCGG_A
Cuts at: 3907 3907
Size: 4687
BspHI T'CATG_A
Cuts at: 3863 3863
Size: 4687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 1735 1949 2251 2632 536
Size: 1199 214 302 381 2591
Fragments arranged by size:
2591 1199 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2977 3031 4517 4682 50
Size: 2927 54 1486 165 55
Fragments arranged by size:
2927 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 188 726 2597 188
Size: 538 1871 2278
Fragments arranged by size:
2278 1871 538
BsrFI r'CCGG_y
Cuts at: 2683 2864 3075 2683
Size: 181 211 4295
Fragments arranged by size:
4295 211 181
BssSI C'ACGA_G
Cuts at: 2956 3316 2956
Size: 360 4327
BstAPI GCAn_nnn'nTGC
Cuts at: 178 609 2062 178
Size: 431 1453 2803
Fragments arranged by size:
2803 1453 431
BstBI TT'CG_AA
Cuts at: 110 110
Size: 4687
BstYI r'GATC_y
Cuts at: 1 1010 1027 1251 1710 2177 2200 2535 2781
Size: 1009 17 224 459 467 23 335 246
Cuts at: 2781 3795 3881 3893 4239 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1014 1009 467 459 449 346 335 246
224 86 23 17 12
BstZ17I GTA'TAC
Cuts at: 1674 1674
Size: 4687
Bsu36I CC'TnA_GG
Cuts at: 328 328
Size: 4687
BtgI C'CryG_G
Cuts at: 125 1188 125
Size: 1063 3624
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2635 2816 2979 2635
Size: 181 163 4343
Fragments arranged by size:
4343 181 163
BtsI GCAGTG_nn'
Cuts at: 1501 1928 1501
Size: 427 4260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1102 1137 1102
Size: 35 4652
DraI TTT'AAA
Cuts at: 197 1684 197
Size: 1487 3200
DraIII CAC_nnn'GTG
Cuts at: 288 288
Size: 4687
DrdI GACnn_nn'nnGTC
Cuts at: 2391 3251 2391
Size: 860 3827
EaeI y'GGCC_r
Cuts at: 145 2270 2444 2835 2862 4209 145
Size: 2125 174 391 27 1347 623
Fragments arranged by size:
2125 1347 623 391 174 27
EagI C'GGCC_G
Cuts at: 2270 2270
Size: 4687
EarI CTCTTCn'nnn_
Cuts at: 262 586 832 2708 2918 262
Size: 324 246 1876 210 2031
Fragments arranged by size:
2031 1876 324 246 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2893 3205 3351 4497 2893
Size: 312 146 1146 3083
Fragments arranged by size:
3083 1146 312 146
EcoICRI GAG'CTC
Cuts at: 351 579 1697 351
Size: 228 1118 3341
Fragments arranged by size:
3341 1118 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 694 1440 2510 2942 3691 3959 360
Size: 334 746 1070 432 749 268 1088
Fragments arranged by size:
1088 1070 749 746 432 334 268
EcoO109I rG'GnC_Cy
Cuts at: 155 156 1603 1824 155
Size: 1 1447 221 3018
Fragments arranged by size:
3018 1447 221 1
EcoRI G'AATT_C
Cuts at: 1689 1689
Size: 4687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 273 2704 273
Size: 2431 2256
FspI TGC'GCA
Cuts at: 2466 2466
Size: 4687
HaeII r_GCGC'y
Cuts at: 1076 2119 2367 3391 4370 1076
Size: 1043 248 1024 979 1393
Fragments arranged by size:
1393 1043 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 295 321 327 353 681 713 942 974 1184
Size: 26 6 26 328 32 229 32 210
Cuts at: 1184 1216 1595 1627 2527 2557 2559 2589 295
Size: 32 379 32 900 30 2 30 2393
Fragments arranged by size:
2393 900 379 328 229 210 32 32
32 32 30 30 26 26 6 2
HincII GTy'rAC
Cuts at: 382 1217 1724 382
Size: 835 507 3345
Fragments arranged by size:
3345 835 507
KasI G'GCGC_C
Cuts at: 2115 2363 2115
Size: 248 4439
KpnI G_GTAC'C
Cuts at: 1705 1705
Size: 4687
MluI A'CGCG_T
Cuts at: 4120 4120
Size: 4687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 647 1279 3358 3542 503
Size: 144 632 2079 184 1648
Fragments arranged by size:
2079 1648 632 184 144
MscI TGG'CCA
Cuts at: 147 2446 147
Size: 2299 2388
MslI CAynn'nnrTG
Cuts at: 234 1231 2674 2801 234
Size: 997 1443 127 2120
Fragments arranged by size:
2120 1443 997 127
MspA1I CmG'CkG
Cuts at: 242 558 774 1389 1650 1796 2110 2470 3485
Size: 316 216 615 261 146 314 360 1015
Cuts at: 3485 3730 242
Size: 245 1199
Fragments arranged by size:
1199 1015 615 360 316 314 261 245
216 146
NaeI GCC'GGC
Cuts at: 2866 2866
Size: 4687
NarI GG'CG_CC
Cuts at: 2116 2364 2116
Size: 248 4439
NcoI C'CATG_G
Cuts at: 125 1188 125
Size: 1063 3624
NdeI CA'TA_TG
Cuts at: 1547 1547
Size: 4687
NgoMIV G'CCGG_C
Cuts at: 2864 2864
Size: 4687
NruI TCG'CGA
Cuts at: 3048 3048
Size: 4687
NspI r_CATG'y
Cuts at: 863 1560 1738 2238 3147 863
Size: 697 178 500 909 2403
Fragments arranged by size:
2403 909 697 500 178
PacI TTA_AT'TAA
Cuts at: 740 740
Size: 4687
PasI CC'CwG_GG
Cuts at: 213 213
Size: 4687
PciI A'CATG_T
Cuts at: 859 1556 3143 859
Size: 697 1587 2403
Fragments arranged by size:
2403 1587 697
PfoI T'CCnGG_A
Cuts at: 1456 1456
Size: 4687
PmeI GTTT'AAAC
Cuts at: 1684 1684
Size: 4687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3826 3858 3826
Size: 32 4655
PpuMI rG'GwC_Cy
Cuts at: 1603 1603
Size: 4687
PspOMI G'GGCC_C
Cuts at: 155 155
Size: 4687
PstI C_TGCA'G
Cuts at: 605 1329 1732 2417 605
Size: 724 403 685 2875
Fragments arranged by size:
2875 724 685 403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4687
PvuII CAG'CTG
Cuts at: 558 774 1650 2110 2470 558
Size: 216 876 460 360 2775
Fragments arranged by size:
2775 876 460 360 216
RsrII CG'GwC_CG
Cuts at: 2880 2880
Size: 4687
SacI G_AGCT'C
Cuts at: 353 581 1699 353
Size: 228 1118 3341
Fragments arranged by size:
3341 1118 228
SalI G'TCGA_C
Cuts at: 1722 1722
Size: 4687
SapI GCTCTTCn'nnn_
Cuts at: 262 586 832 2708 2918 262
Size: 324 246 1876 210 2031
Fragments arranged by size:
2031 1876 324 246 210
SbfI CC_TGCA'GG
Cuts at: 1732 1732
Size: 4687
ScaI AGT'ACT
Cuts at: 4204 4204
Size: 4687
SfcI C'TryA_G
Cuts at: 33 601 1325 1728 2413 3408 3599 33
Size: 568 724 403 685 995 191 1121
Fragments arranged by size:
1121 995 724 685 568 403 191
SfoI GGC'GCC
Cuts at: 2117 2365 2117
Size: 248 4439
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4687
SmaI CCC'GGG
Cuts at: 1707 1707
Size: 4687
SmlI C'TyrA_G
Cuts at: 457 471 1840 2755 3249 3511 457
Size: 14 1369 915 494 262 1633
Fragments arranged by size:
1633 1369 915 494 262 14
SnaBI TAC'GTA
Cuts at: 1677 1677
Size: 4687
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4687
SphI G_CATG'C
Cuts at: 1738 2238 1738
Size: 500 4187
SspI AAT'ATT
Cuts at: 488 488
Size: 4687
StyI C'CwwG_G
Cuts at: 125 1113 1161 1188 1566 1819 2173 125
Size: 988 48 27 378 253 354 2639
Fragments arranged by size:
2639 988 378 354 253 48 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 246 587 1174 1609 2268 2401 2860 3071 246
Size: 341 587 435 659 133 459 211 1862
Fragments arranged by size:
1862 659 587 459 435 341 211 133
TatI w'GTAC_w
Cuts at: 4202 4202
Size: 4687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4204 4236 4204
Size: 32 4655
Tth111I GACn'n_nGTC
Cuts at: 2482 2482
Size: 4687
XbaI T'CTAG_A
Cuts at: 1176 1716 1176
Size: 540 4147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2180 2180
Size: 4687
XmaI C'CCGG_G
Cuts at: 1705 1705
Size: 4687
XmnI GAAnn'nnTTC
Cuts at: 1355 1355
Size: 4687
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NspI PacI PasI PciI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII
BstXI ClaI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NsiI PflMI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI
StuI SwaI XhoI ZraI