Restriction map for
pF1KSDA0375
(Circular) (Six-base) MAPSORT of: pF1KSDA0375.seq Check: 5337 from: 1 to: 7687
pF1KSDA0375 7687 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 214 530 214
Size: 316 7371
AatII G_ACGT'C
Cuts at: 1605 3302 1605
Size: 1697 5990
AccI GT'mk_AC
Cuts at: 1565 3965 4723 1565
Size: 2400 758 4529
Fragments arranged by size:
4529 2400 758
Acc65I G'GTAC_C
Cuts at: 3938 4701 3938
Size: 763 6924
AclI AA'CG_TT
Cuts at: 7527 7527
Size: 7687
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 960 3082 3256 3612 5510 5942 6691 6959 960
Size: 2122 174 356 1898 432 749 268 1688
Fragments arranged by size:
2122 1898 1688 749 432 356 268 174
AfeI AGC'GCT
Cuts at: 1609 1609
Size: 7687
AflIII A'CryG_T
Cuts at: 5037 6143 7120 5037
Size: 1106 977 5604
Fragments arranged by size:
5604 1106 977
AgeI A'CCGG_T
Cuts at: 6075 6075
Size: 7687
AleI CACnn'nnGTG
Cuts at: 2006 2006
Size: 7687
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 804 836 804
Size: 32 7655
AlwNI CAG_nnn'CTG
Cuts at: 692 1075 1255 1423 1531 1534 2077 3612 3717
Size: 383 180 168 108 3 543 1535 105
Cuts at: 3717 4488 4990 6559 692
Size: 771 502 1569 1820
Fragments arranged by size:
1820 1569 1535 771 543 502 383 180
168 108 105 3
ApaI G_GGCC'C
Cuts at: 1543 1708 1881 2262 2498 3759 3775 3915 4220
Size: 165 173 381 236 1261 16 140 305
Cuts at: 4220 1543
Size: 5010
Fragments arranged by size:
5010 1261 381 305 236 173 165 140
16
ApaLI G'TGCA_C
Cuts at: 184 6457 184
Size: 6273 1414
ApoI r'AATT_y
Cuts at: 4689 7040 7296 4689
Size: 2351 256 5080
Fragments arranged by size:
5080 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7687
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7687
AvaI C'yCGr_G
Cuts at: 2255 2390 2738 3175 3752 3758 3914 4076 4705
Size: 135 348 437 577 6 156 162 629
Cuts at: 4705 2255
Size: 5237
Fragments arranged by size:
5237 629 577 437 348 162 156 135
6
AvrII C'CTAG_G
Cuts at: 300 300
Size: 7687
BamHI G'GATC_C
Cuts at: 2067 3171 4710 2067
Size: 1104 1539 5044
Fragments arranged by size:
5044 1539 1104
BanI G'GyrC_C
Cuts at: 682 1003 1078 2146 2407 3823 3938 4611 4701
Size: 321 75 1068 261 1416 115 673 90
Cuts at: 4701 5115 5363 5398 682
Size: 414 248 35 2971
Fragments arranged by size:
2971 1416 1068 673 414 321 261 248
115 90 75 35
BanII G_rGCy'C
Cuts at: 414 1543 1663 1708 1881 2095 2262 2498 2701
Size: 1129 120 45 173 214 167 236 203
Cuts at: 2701 3214 3281 3419 3759 3775 3895 3915 3958
Size: 513 67 138 340 16 120 20 43
Cuts at: 3958 4168 4220 4421 4699 5729 414
Size: 210 52 201 278 1030 2372
Fragments arranged by size:
2372 1129 1030 513 340 278 236 214
210 203 201 173 167 138 120 120
67 52 45 43 20 16
BbeI G_GCGC'C
Cuts at: 1082 5119 5367 1082
Size: 4037 248 3402
Fragments arranged by size:
4037 3402 248
BbsI GAAGACnn'nnnn_
Cuts at: 3518 3518
Size: 7687
BbvCI CC'TCA_GC
Cuts at: 1591 2716 4100 4192 1591
Size: 1125 1384 92 5086
Fragments arranged by size:
5086 1384 1125 92
BciVI GTATCCnnnnn_n'
Cuts at: 660 5583 6346 7687 660
Size: 4923 763 1341 660
Fragments arranged by size:
4923 1341 763 660
BclI T'GATC_A
Cuts at: 5205 5205
Size: 7687
BfrBI ATG'CAT
Cuts at: 999 999
Size: 7687
BglI GCCn_nnn'nGGC
Cuts at: 1364 1765 1932 2294 2326 3770 3910 1364
Size: 401 167 362 32 1444 140 5141
Fragments arranged by size:
5141 1444 401 362 167 140 32
BglII A'GATC_T
Cuts at: 5200 7239 5200
Size: 2039 5648
BlpI GC'TnA_GC
Cuts at: 2057 4354 4522 4797 2057
Size: 2297 168 275 4947
Fragments arranged by size:
4947 2297 275 168
Bme1580I G_kGCm'C
Cuts at: 188 1246 1543 1708 1881 1897 2262 2498 3250
Size: 1058 297 165 173 16 365 236 752
Cuts at: 3250 3759 3775 3915 4220 4616 5310 5403 6461
Size: 509 16 140 305 396 694 93 1058
Cuts at: 6461 188
Size: 1414
Fragments arranged by size:
1414 1058 1058 752 694 509 396 365
305 297 236 173 165 140 93 16
16
BmgBI CAC'GTC
Cuts at: 3783 3783
Size: 7687
BmrI ACTGGGnnnn_n'
Cuts at: 1681 2048 2768 4897 4978 5313 7459 1681
Size: 367 720 2129 81 335 2146 1909
Fragments arranged by size:
2146 2129 1909 720 367 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 175 3575 4461 6052 175
Size: 3400 886 1591 1810
Fragments arranged by size:
3400 1810 1591 886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 329 428 644 1808 1989 3023 3289 3499
Size: 159 99 216 1164 181 1034 266 210
Cuts at: 3499 3797 4289 4446 4586 170
Size: 298 492 157 140 3271
Fragments arranged by size:
3271 1164 1034 492 298 266 216 210
181 159 157 140 99
Bpu10I CC'TnA_GC
Cuts at: 269 858 1196 1591 2716 3894 4100 4192 269
Size: 589 338 395 1125 1178 206 92 3764
Fragments arranged by size:
3764 1178 1125 589 395 338 206 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1573 1716 2327 2783 2791 3265 4861 5740 6234
Size: 143 611 456 8 474 1596 879 494
Cuts at: 6234 6532 1573
Size: 298 2728
Fragments arranged by size:
2728 1596 879 611 494 474 456 298
143 8
BsaI GGTCTCn'nnnn_
Cuts at: 148 2428 7391 148
Size: 2280 4963 444
Fragments arranged by size:
4963 2280 444
BsaAI yAC'GTr
Cuts at: 4626 4677 5038 4626
Size: 51 361 7275
Fragments arranged by size:
7275 361 51
BsaBI GATnn'nnATC
Cuts at: 6 5199 7238 6
Size: 5193 2039 455
Fragments arranged by size:
5193 2039 455
BsaHI Gr'CG_yC
Cuts at: 1079 1602 3299 5116 5364 6068 7564 1079
Size: 523 1697 1817 248 704 1496 1202
Fragments arranged by size:
1817 1697 1496 1202 704 523 248
BsaWI w'CCGG_w
Cuts at: 2474 3470 4874 5395 6075 6349 6496 6907 2474
Size: 996 1404 521 680 274 147 411 3254
Fragments arranged by size:
3254 1404 996 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 747 777 1201 1231 3472 3502 4626 4656 747
Size: 30 424 30 2241 30 1124 30 3778
Fragments arranged by size:
3778 2241 1124 424 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 930 1821 3874 4135 4583 930
Size: 891 2053 261 448 4034
Fragments arranged by size:
4034 2053 891 448 261
BseYI C'CCAG_C
Cuts at: 273 284 506 1245 1441 1862 2094 2276 2355
Size: 11 222 739 196 421 232 182 79
Cuts at: 2355 2419 2497 2615 3192 4310 4449 4554 5110
Size: 64 78 118 577 1118 139 105 556
Cuts at: 5110 5868 6447 6943 273
Size: 758 579 496 1017
Fragments arranged by size:
1118 1017 758 739 579 577 556 496
421 232 222 196 182 139 118 105
79 78 64 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 341 3575 4501 319
Size: 22 3234 926 3505
Fragments arranged by size:
3505 3234 926 22
BsiEI CG_ry'CG
Cuts at: 104 5227 5273 6483 104
Size: 5123 46 1210 1308
Fragments arranged by size:
5123 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 188 244 2530 3419 3958 4699 5477 5667 6461
Size: 56 2286 889 539 741 778 190 794
Cuts at: 6461 188
Size: 1414
Fragments arranged by size:
2286 1414 889 794 778 741 539 190
56
BsmBI CGTCTCn'nnnn_
Cuts at: 3773 3773
Size: 7687
Bsp1286I G_dGCh'C
Cuts at: 188 244 414 1246 1543 1663 1708 1881 1897
Size: 56 170 832 297 120 45 173 16
Cuts at: 1897 2095 2262 2498 2530 2701 3214 3250 3281
Size: 198 167 236 32 171 513 36 31
Cuts at: 3281 3419 3759 3775 3895 3915 3958 4168 4220
Size: 138 340 16 120 20 43 210 52
Cuts at: 4220 4421 4616 4699 5310 5403 5477 5667 5729
Size: 201 195 83 611 93 74 190 62
Cuts at: 5729 6461 188
Size: 732 1414
Fragments arranged by size:
1414 832 732 611 513 340 297 236
210 201 198 195 190 173 171 170
167 138 120 120 93 83 74 62
56 52 45 43 36 32 31 20
16 16
BspEI T'CCGG_A
Cuts at: 2474 6907 2474
Size: 4433 3254
BspHI T'CATG_A
Cuts at: 6863 6863
Size: 7687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 214 530 3004 3101 3725 4735 4949 5251 5632
Size: 316 2474 97 624 1010 214 302 381
Cuts at: 5632 214
Size: 2269
Fragments arranged by size:
2474 2269 1010 624 381 316 302 214
97
BsrBI CCG'CTC
Cuts at: 50 3807 5977 6031 7517 7682 50
Size: 3757 2170 54 1486 165 55
Fragments arranged by size:
3757 2170 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 5597 5597
Size: 7687
BsrFI r'CCGG_y
Cuts at: 698 2663 2957 3653 3928 4507 5683 5864 6075
Size: 1965 294 696 275 579 1176 181 211
Cuts at: 6075 698
Size: 2310
Fragments arranged by size:
2310 1965 1176 696 579 294 275 211
181
BssSI C'ACGA_G
Cuts at: 4001 5956 6316 4001
Size: 1955 360 5372
Fragments arranged by size:
5372 1955 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1531 3657 5062 1531
Size: 2126 1405 4156
Fragments arranged by size:
4156 2126 1405
BstBI TT'CG_AA
Cuts at: 110 110
Size: 7687
BstEII G'GTnAC_C
Cuts at: 469 969 1746 469
Size: 500 777 6410
Fragments arranged by size:
6410 777 500
BstXI CCAn_nnnn'nTGG
Cuts at: 526 1929 2244 526
Size: 1403 315 5969
Fragments arranged by size:
5969 1403 315
BstYI r'GATC_y
Cuts at: 1 1092 2067 3171 4710 5177 5200 5535 5781
Size: 1091 975 1104 1539 467 23 335 246
Cuts at: 5781 6795 6881 6893 7239 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1539 1104 1091 1014 975 467 449 346
335 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1566 1566
Size: 7687
Bsu36I CC'TnA_GG
Cuts at: 2364 2364
Size: 7687
BtgI C'CryG_G
Cuts at: 125 598 920 1715 2572 2670 3328 4590 125
Size: 473 322 795 857 98 658 1262 3222
Fragments arranged by size:
3222 1262 857 795 658 473 322 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2432 3114 5635 5816 5979 2432
Size: 682 2521 181 163 4140
Fragments arranged by size:
4140 2521 682 181 163
BtsI GCAGTG_nn'
Cuts at: 186 214 682 690 768 870 1256 1511 2197
Size: 28 468 8 78 102 386 255 686
Cuts at: 2197 2981 4928 186
Size: 784 1947 2945
Fragments arranged by size:
2945 1947 784 686 468 386 255 102
78 28 8
ClaI AT'CG_AT
Cuts at: 3971 3971
Size: 7687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1533 1568 2834 2869 1533
Size: 35 1266 35 6351
Fragments arranged by size:
6351 1266 35 35
DraI TTT'AAA
Cuts at: 4684 4684
Size: 7687
DraIII CAC_nnn'GTG
Cuts at: 1234 1813 2525 1234
Size: 579 712 6396
Fragments arranged by size:
6396 712 579
DrdI GACnn_nn'nnGTC
Cuts at: 5391 6251 5391
Size: 860 6827
EaeI y'GGCC_r
Cuts at: 534 700 1298 1718 1766 2246 2443 2762 2967
Size: 166 598 420 48 480 197 319 205
Cuts at: 2967 4184 4502 5270 5444 5835 5862 7209 534
Size: 1217 318 768 174 391 27 1347 1012
Fragments arranged by size:
1347 1217 1012 768 598 480 420 391
319 318 205 197 174 166 48 27
EagI C'GGCC_G
Cuts at: 5270 5270
Size: 7687
EarI CTCTTCn'nnn_
Cuts at: 393 1056 1194 1374 2514 3581 3836 3842 3851
Size: 663 138 180 1140 1067 255 6 9
Cuts at: 3851 3863 5708 5918 393
Size: 12 1845 210 2162
Fragments arranged by size:
2162 1845 1140 1067 663 255 210 180
138 12 9 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1642 5893 6205 6351 7497 1642
Size: 4251 312 146 1146 1832
Fragments arranged by size:
4251 1832 1146 312 146
EcoICRI GAG'CTC
Cuts at: 3417 3956 4697 3417
Size: 539 741 6407
Fragments arranged by size:
6407 741 539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 329 428 644 960 1808 1989 3023 3082
Size: 159 99 216 316 848 181 1034 59
Cuts at: 3082 3256 3289 3499 3612 3797 4289 4446 4586
Size: 174 33 210 113 185 492 157 140
Cuts at: 4586 5510 5942 6691 6959 170
Size: 924 432 749 268 898
Fragments arranged by size:
1034 924 898 848 749 492 432 316
268 216 210 185 181 174 159 157
140 113 99 59 33
EcoO109I rG'GnC_Cy
Cuts at: 403 745 1420 1438 1540 1704 1705 1877 1878
Size: 342 675 18 102 164 1 172 1
Cuts at: 1878 1996 2047 2074 2259 3012 3375 3714 3771
Size: 118 51 27 185 753 363 339 57
Cuts at: 3771 3772 4054 4125 4216 4250 4824 403
Size: 1 282 71 91 34 574 3266
Fragments arranged by size:
3266 753 675 574 363 342 339 282
185 172 164 118 102 91 71 57
51 34 27 18 1 1 1
EcoRI G'AATT_C
Cuts at: 4689 4689
Size: 7687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5704 5704
Size: 7687
FspI TGC'GCA
Cuts at: 5466 5466
Size: 7687
HaeII r_GCGC'y
Cuts at: 1082 1611 5119 5367 6391 7370 1082
Size: 529 3508 248 1024 979 1399
Fragments arranged by size:
3508 1399 1024 979 529 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 372 404 2699 2731 2860 2892 3055 3087 4185
Size: 32 2295 32 129 32 163 32 1098
Cuts at: 4185 4217 4626 4658 5527 5557 5559 5589 372
Size: 32 409 32 869 30 2 30 2470
Fragments arranged by size:
2470 2295 1098 869 409 163 129 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 3126 4724 3126
Size: 1598 6089
HindIII A'AGCT_T
Cuts at: 2682 2682
Size: 7687
HpaI GTT'AAC
Cuts at: 3126 3126
Size: 7687
KasI G'GCGC_C
Cuts at: 1078 5115 5363 1078
Size: 4037 248 3402
Fragments arranged by size:
4037 3402 248
KpnI G_GTAC'C
Cuts at: 3942 4705 3942
Size: 763 6924
MluI A'CGCG_T
Cuts at: 7120 7120
Size: 7687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4401 4675 6358 6542 4401
Size: 274 1683 184 5546
Fragments arranged by size:
5546 1683 274 184
MscI TGG'CCA
Cuts at: 536 1300 1768 2248 2764 2969 4504 5446 536
Size: 764 468 480 516 205 1535 942 2777
Fragments arranged by size:
2777 1535 942 764 516 480 468 205
MslI CAynn'nnrTG
Cuts at: 524 2006 3133 5674 5801 524
Size: 1482 1127 2541 127 2410
Fragments arranged by size:
2541 2410 1482 1127 127
MspA1I CmG'CkG
Cuts at: 567 878 910 1531 2061 2244 3043 3681 3747
Size: 311 32 621 530 183 799 638 66
Cuts at: 3747 4222 4314 4521 4796 5110 5470 6485 6730
Size: 475 92 207 275 314 360 1015 245
Cuts at: 6730 567
Size: 1524
Fragments arranged by size:
1524 1015 799 638 621 530 475 360
314 311 275 245 207 183 92 66
32
NaeI GCC'GGC
Cuts at: 700 2959 3930 5866 700
Size: 2259 971 1936 2521
Fragments arranged by size:
2521 2259 1936 971
NarI GG'CG_CC
Cuts at: 1079 5116 5364 1079
Size: 4037 248 3402
Fragments arranged by size:
4037 3402 248
NcoI C'CATG_G
Cuts at: 125 598 920 1715 2572 2670 3328 125
Size: 473 322 795 857 98 658 4484
Fragments arranged by size:
4484 857 795 658 473 322 98
NgoMIV G'CCGG_C
Cuts at: 698 2957 3928 5864 698
Size: 2259 971 1936 2521
Fragments arranged by size:
2521 2259 1936 971
NruI TCG'CGA
Cuts at: 6048 6048
Size: 7687
NsiI A_TGCA'T
Cuts at: 1001 1001
Size: 7687
NspI r_CATG'y
Cuts at: 3327 4738 5238 6147 3327
Size: 1411 500 909 4867
Fragments arranged by size:
4867 1411 909 500
PasI CC'CwG_GG
Cuts at: 2086 2454 3539 4128 2086
Size: 368 1085 589 5645
Fragments arranged by size:
5645 1085 589 368
PciI A'CATG_T
Cuts at: 6143 6143
Size: 7687
PflMI CCAn_nnn'nTGG
Cuts at: 466 577 921 1423 4383 4500 4657 466
Size: 111 344 502 2960 117 157 3496
Fragments arranged by size:
3496 2960 502 344 157 117 111
PfoI T'CCnGG_A
Cuts at: 345 3008 345
Size: 2663 5024
PmeI GTTT'AAAC
Cuts at: 4684 4684
Size: 7687
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6826 6858 6826
Size: 32 7655
PpuMI rG'GwC_Cy
Cuts at: 745 1996 3012 3375 3714 4054 745
Size: 1251 1016 363 339 340 4378
Fragments arranged by size:
4378 1251 1016 363 340 339
PshAI GACnn'nnGTC
Cuts at: 1964 1964
Size: 7687
PspOMI G'GGCC_C
Cuts at: 1539 1704 1877 2258 2494 3755 3771 3911 4216
Size: 165 173 381 236 1261 16 140 305
Cuts at: 4216 1539
Size: 5010
Fragments arranged by size:
5010 1261 381 305 236 173 165 140
16
PstI C_TGCA'G
Cuts at: 443 883 1045 1063 1100 1637 1713 2302 3746
Size: 440 162 18 37 537 76 589 1444
Cuts at: 3746 4436 4588 4732 5417 443
Size: 690 152 144 685 2713
Fragments arranged by size:
2713 1444 690 685 589 537 440 162
152 144 76 37 18
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7687
PvuII CAG'CTG
Cuts at: 567 910 1531 2061 2244 3043 3747 4521 5110
Size: 343 621 530 183 799 704 774 589
Cuts at: 5110 5470 567
Size: 360 2784
Fragments arranged by size:
2784 799 774 704 621 589 530 360
343 183
RsrII CG'GwC_CG
Cuts at: 5880 5880
Size: 7687
SacI G_AGCT'C
Cuts at: 3419 3958 4699 3419
Size: 539 741 6407
Fragments arranged by size:
6407 741 539
SalI G'TCGA_C
Cuts at: 4722 4722
Size: 7687
SanDI GG'GwC_CC
Cuts at: 1996 1996
Size: 7687
SapI GCTCTTCn'nnn_
Cuts at: 1374 3581 5708 5918 1374
Size: 2207 2127 210 3143
Fragments arranged by size:
3143 2207 2127 210
SbfI CC_TGCA'GG
Cuts at: 4436 4732 4436
Size: 296 7391
ScaI AGT'ACT
Cuts at: 3241 7204 3241
Size: 3963 3724
SexAI A'CCwGG_T
Cuts at: 747 2847 3227 747
Size: 2100 380 5207
Fragments arranged by size:
5207 2100 380
SfcI C'TryA_G
Cuts at: 33 365 439 879 1041 1059 1096 1633 1709
Size: 332 74 440 162 18 37 537 76
Cuts at: 1709 1902 2172 2283 2298 3604 3742 4432 4584
Size: 193 270 111 15 1306 138 690 152
Cuts at: 4584 4728 5413 6408 6599 33
Size: 144 685 995 191 1121
Fragments arranged by size:
1306 1121 995 690 685 537 440 332
270 193 191 162 152 144 138 111
76 74 37 18 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 3770 3770
Size: 7687
SfoI GGC'GCC
Cuts at: 1080 5117 5365 1080
Size: 4037 248 3402
Fragments arranged by size:
4037 3402 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7687
SmaI CCC'GGG
Cuts at: 2257 2740 3177 3754 3760 4707 2257
Size: 483 437 577 6 947 5237
Fragments arranged by size:
5237 947 577 483 437 6
SmlI C'TyrA_G
Cuts at: 1552 1695 2342 2798 2806 3280 4840 5755 6249
Size: 143 647 456 8 474 1560 915 494
Cuts at: 6249 6511 1552
Size: 262 2728
Fragments arranged by size:
2728 1560 915 647 494 474 456 262
143 8
SnaBI TAC'GTA
Cuts at: 4677 4677
Size: 7687
SpeI A'CTAG_T
Cuts at: 67 4380 67
Size: 4313 3374
SphI G_CATG'C
Cuts at: 4738 5238 4738
Size: 500 7187
SrfI GCCC'GGGC
Cuts at: 2257 3754 2257
Size: 1497 6190
StuI AGG'CCT
Cuts at: 3286 4105 3286
Size: 819 6868
StyI C'CwwG_G
Cuts at: 125 300 598 662 835 920 1715 1769 2249
Size: 175 298 64 173 85 795 54 480
Cuts at: 2249 2572 2670 3328 3371 3798 4421 4819 5173
Size: 323 98 658 43 427 623 398 354
Cuts at: 5173 125
Size: 2639
Fragments arranged by size:
2639 795 658 623 480 427 398 354
323 298 175 173 98 85 64 54
43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 298 442 520 1534 1752 1863 1935 1996 2885
Size: 144 78 1014 218 111 72 61 889
Cuts at: 2885 3811 5268 5401 5860 6071 298
Size: 926 1457 133 459 211 1914
Fragments arranged by size:
1914 1457 1014 926 889 459 218 211
144 133 111 78 72 61
TatI w'GTAC_w
Cuts at: 3239 3291 7202 3239
Size: 52 3911 3724
Fragments arranged by size:
3911 3724 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 747 779 2189 2221 2257 2289 7204 7236 747
Size: 32 1410 32 36 32 4915 32 1198
Fragments arranged by size:
4915 1410 1198 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5482 5482
Size: 7687
XbaI T'CTAG_A
Cuts at: 4716 4716
Size: 7687
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1072 1970 3744 3772 5180 1072
Size: 898 1774 28 1408 3579
Fragments arranged by size:
3579 1774 1408 898 28
XmaI C'CCGG_G
Cuts at: 2255 2738 3175 3752 3758 4705 2255
Size: 483 437 577 6 947 5237
Fragments arranged by size:
5237 947 577 483 437 6
XmnI GAAnn'nnTTC
Cuts at: 3201 3201
Size: 7687
ZraI GAC'GTC
Cuts at: 1603 3300 1603
Size: 1697 5990
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AlfI AscI BaeI BcgI BmtI BsiWI
BsmI BsrGI BssHII EcoNI EcoRV FseI FspAI MfeI
NdeI NheI NotI PacI PmlI PsiI PspXI PsrI
SacII SgrAI SspI SwaI XhoI