Restriction map for
pF1KSDA0372
(Circular) (Six-base) MAPSORT of: pF1KSDA0372.seq Check: 2140 from: 1 to: 7829
pF1KSDA0372 7829 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2204 2446 4404 4867 2204
Size: 242 1958 463 5166
Fragments arranged by size:
5166 1958 463 242
Acc65I G'GTAC_C
Cuts at: 4697 4845 4697
Size: 148 7681
AclI AA'CG_TT
Cuts at: 7669 7669
Size: 7829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 1214 1406 1594 2859 2927 3592 4175 5654
Size: 70 192 188 1265 68 665 583 1479
Cuts at: 5654 6086 6833 7101 1144
Size: 432 747 268 1872
Fragments arranged by size:
1872 1479 1265 747 665 583 432 268
192 188 70 68
AflII C'TTAA_G
Cuts at: 1950 2533 1950
Size: 583 7246
AflIII A'CryG_T
Cuts at: 4347 5181 6285 7262 4347
Size: 834 1104 977 4914
Fragments arranged by size:
4914 1104 977 834
AgeI A'CCGG_T
Cuts at: 6217 6217
Size: 7829
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 7829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2962 2962
Size: 7829
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1277 1308 1309 1340 1999 2031 1277
Size: 31 1 31 659 32 7075
Fragments arranged by size:
7075 659 32 31 31 1
AlwNI CAG_nnn'CTG
Cuts at: 624 1879 4276 4341 5134 6701 624
Size: 1255 2397 65 793 1567 1752
Fragments arranged by size:
2397 1752 1567 1255 793 65
ApaLI G'TGCA_C
Cuts at: 6599 6599
Size: 7829
ApoI r'AATT_y
Cuts at: 766 3050 4069 4179 4833 7182 7438 766
Size: 2284 1019 110 654 2349 256 1157
Fragments arranged by size:
2349 2284 1157 1019 654 256 110
AseI AT'TA_AT
Cuts at: 20 2477 2702 20
Size: 2457 225 5147
Fragments arranged by size:
5147 2457 225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7829
AvaI C'yCGr_G
Cuts at: 3725 4849 3725
Size: 1124 6705
AvrII C'CTAG_G
Cuts at: 2058 2058
Size: 7829
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1466 1499 4336 4369 4681 4714 1466
Size: 33 2837 33 312 33 4581
Fragments arranged by size:
4581 2837 312 33 33 33
BamHI G'GATC_C
Cuts at: 4854 4854
Size: 7829
BanI G'GyrC_C
Cuts at: 3608 4697 4845 5259 5507 5542 3608
Size: 1089 148 414 248 35 5895
Fragments arranged by size:
5895 1089 414 248 148 35
BanII G_rGCy'C
Cuts at: 1464 1665 2399 4843 5873 1464
Size: 201 734 2444 1030 3420
Fragments arranged by size:
3420 2444 1030 734 201
BbeI G_GCGC'C
Cuts at: 5263 5511 5263
Size: 248 7581
BbsI GAAGACnn'nnnn_
Cuts at: 151 1174 1361 3173 3287 151
Size: 1023 187 1812 114 4693
Fragments arranged by size:
4693 1812 1023 187 114
BbvCI CC'TCA_GC
Cuts at: 2840 2840
Size: 7829
BciVI GTATCCnnnnn_n'
Cuts at: 848 1584 4676 5727 6488 7829 848
Size: 736 3092 1051 761 1341 848
Fragments arranged by size:
3092 1341 1051 848 761 736
BclI T'GATC_A
Cuts at: 294 1254 2096 4197 4506 5349 294
Size: 960 842 2101 309 843 2774
Fragments arranged by size:
2774 2101 960 843 842 309
BfrBI ATG'CAT
Cuts at: 1083 2901 3130 1083
Size: 1818 229 5782
Fragments arranged by size:
5782 1818 229
BglI GCCn_nnn'nGGC
Cuts at: 953 1402 953
Size: 449 7380
BglII A'GATC_T
Cuts at: 5344 7381 5344
Size: 2037 5792
BlpI GC'TnA_GC
Cuts at: 2663 4941 2663
Size: 2278 5551
Bme1580I G_kGCm'C
Cuts at: 5454 5547 6603 5454
Size: 93 1056 6680
Fragments arranged by size:
6680 1056 93
BmgBI CAC'GTC
Cuts at: 4746 4746
Size: 7829
BmrI ACTGGGnnnn_n'
Cuts at: 703 5041 5122 5457 7601 703
Size: 4338 81 335 2144 931
Fragments arranged by size:
4338 2144 931 335 81
BmtI G_CTAG'C
Cuts at: 2284 2284
Size: 7829
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6196 6196
Size: 7829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1736 2566 2980 3908 4270 1736
Size: 830 414 928 362 5295
Fragments arranged by size:
5295 928 830 414 362
Bpu10I CC'TnA_GC
Cuts at: 401 957 1828 2840 401
Size: 556 871 1012 5390
Fragments arranged by size:
5390 1012 871 556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 1486 1853 4431 5005 5884 6376 6674 1420
Size: 66 367 2578 574 879 492 298 2575
Fragments arranged by size:
2578 2575 879 574 492 367 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 3719 4112 7533 3719
Size: 393 3421 4015
Fragments arranged by size:
4015 3421 393
BsaAI yAC'GTr
Cuts at: 4821 5182 4821
Size: 361 7468
BsaBI GATnn'nnATC
Cuts at: 6 817 5343 7380 6
Size: 811 4526 2037 455
Fragments arranged by size:
4526 2037 811 455
BsaHI Gr'CG_yC
Cuts at: 4632 5260 5508 6210 7706 4632
Size: 628 248 702 1496 4755
Fragments arranged by size:
4755 1496 702 628 248
BsaWI w'CCGG_w
Cuts at: 5018 5539 6217 6491 6638 7049 5018
Size: 521 678 274 147 411 5798
Fragments arranged by size:
5798 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3755 3785 3755
Size: 30 7799
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1973 2389 1973
Size: 416 7413
BseYI C'CCAG_C
Cuts at: 2284 3066 3946 5254 6012 6589 7085 2284
Size: 782 880 1308 758 577 496 3028
Fragments arranged by size:
3028 1308 880 782 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 596 1023 2922 3872 4155 4673 596
Size: 427 1899 950 283 518 3752
Fragments arranged by size:
3752 1899 950 518 427 283
BsiEI CG_ry'CG
Cuts at: 104 5371 5417 6625 104
Size: 5267 46 1208 1308
Fragments arranged by size:
5267 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1464 1665 2244 2399 3516 4843 5621 5811 6603
Size: 201 579 155 1117 1327 778 190 792
Cuts at: 6603 1464
Size: 2690
Fragments arranged by size:
2690 1327 1117 792 778 579 201 190
155
BsiWI C'GTAC_G
Cuts at: 1246 1246
Size: 7829
BsmI GAATG_Cn'
Cuts at: 693 1863 2152 2616 3009 693
Size: 1170 289 464 393 5513
Fragments arranged by size:
5513 1170 464 393 289
BsmBI CGTCTCn'nnnn_
Cuts at: 1631 1631
Size: 7829
Bsp1286I G_dGCh'C
Cuts at: 1464 1665 2244 2399 3516 4843 5454 5547 5621
Size: 201 579 155 1117 1327 611 93 74
Cuts at: 5621 5811 5873 6603 1464
Size: 190 62 730 2690
Fragments arranged by size:
2690 1327 1117 730 611 579 201 190
155 93 74 62
BspEI T'CCGG_A
Cuts at: 7049 7049
Size: 7829
BspHI T'CATG_A
Cuts at: 774 2766 3311 7005 774
Size: 1992 545 3694 1598
Fragments arranged by size:
3694 1992 1598 545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4879 5093 5395 5776 4879
Size: 214 302 381 6932
Fragments arranged by size:
6932 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6121 6175 7659 7824 50
Size: 6071 54 1484 165 55
Fragments arranged by size:
6071 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2944 3451 3533 5741 2944
Size: 507 82 2208 5032
Fragments arranged by size:
5032 2208 507 82
BsrFI r'CCGG_y
Cuts at: 5827 6008 6217 5827
Size: 181 209 7439
Fragments arranged by size:
7439 209 181
BsrGI T'GTAC_A
Cuts at: 831 3827 831
Size: 2996 4833
BssSI C'ACGA_G
Cuts at: 1664 6100 6458 1664
Size: 4436 358 3035
Fragments arranged by size:
4436 3035 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3127 3742 4471 4687 5206 3127
Size: 615 729 216 519 5750
Fragments arranged by size:
5750 729 615 519 216
BstBI TT'CG_AA
Cuts at: 110 6190 110
Size: 6080 1749
BstEII G'GTnAC_C
Cuts at: 3697 3697
Size: 7829
BstYI r'GATC_y
Cuts at: 1 438 495 2868 4854 5321 5344 5679 5925
Size: 437 57 2373 1986 467 23 335 246
Cuts at: 5925 6937 7023 7035 7381 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2373 1986 1012 467 449 437 346 335
246 86 57 23 12
BtgI C'CryG_G
Cuts at: 2864 2864
Size: 7829
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5779 5960 6123 5779
Size: 181 163 7485
Fragments arranged by size:
7485 181 163
BtsI GCAGTG_nn'
Cuts at: 1880 2582 3654 3830 4342 5072 1880
Size: 702 1072 176 512 730 4637
Fragments arranged by size:
4637 1072 730 702 512 176
DraI TTT'AAA
Cuts at: 2132 3462 4184 4828 2132
Size: 1330 722 644 5133
Fragments arranged by size:
5133 1330 722 644
DrdI GACnn_nn'nnGTC
Cuts at: 5535 6393 5535
Size: 858 6971
EaeI y'GGCC_r
Cuts at: 4513 5414 5588 5979 6006 7351 4513
Size: 901 174 391 27 1345 4991
Fragments arranged by size:
4991 1345 901 391 174 27
EagI C'GGCC_G
Cuts at: 5414 5414
Size: 7829
EarI CTCTTCn'nnn_
Cuts at: 581 1233 1371 1526 2878 5852 6062 581
Size: 652 138 155 1352 2974 210 2348
Fragments arranged by size:
2974 2348 1352 652 210 155 138
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6037 6347 6493 7639 6037
Size: 310 146 1146 6227
Fragments arranged by size:
6227 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1462 1663 2397 4841 1462
Size: 201 734 2444 4450
Fragments arranged by size:
4450 2444 734 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1144 1214 1406 1594 1736 2566 2859 2927 2980
Size: 70 192 188 142 830 293 68 53
Cuts at: 2980 3592 3908 4175 4270 5654 6086 6833 7101
Size: 612 316 267 95 1384 432 747 268
Cuts at: 7101 1144
Size: 1872
Fragments arranged by size:
1872 1384 830 747 612 432 316 293
268 267 192 188 142 95 70 68
53
EcoNI CCTnn'n_nnAGG
Cuts at: 1827 1827
Size: 7829
EcoO109I rG'GnC_Cy
Cuts at: 4968 4968
Size: 7829
EcoRI G'AATT_C
Cuts at: 3050 4833 3050
Size: 1783 6046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 426 1120 3574 4575 5848 426
Size: 694 2454 1001 1273 2407
Fragments arranged by size:
2454 2407 1273 1001 694
FspI TGC'GCA
Cuts at: 5610 5610
Size: 7829
HaeII r_GCGC'y
Cuts at: 5263 5511 6533 7512 5263
Size: 248 1022 979 5580
Fragments arranged by size:
5580 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 158 190 1210 1242 1348 1380 1696 1728 4617
Size: 32 1020 32 106 32 316 32 2889
Cuts at: 4617 4649 5671 5701 5703 5733 158
Size: 32 1022 30 2 30 2254
Fragments arranged by size:
2889 2254 1022 1020 316 106 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 456 1731 3260 4868 456
Size: 1275 1529 1608 3417
Fragments arranged by size:
3417 1608 1529 1275
HindIII A'AGCT_T
Cuts at: 197 1302 4552 4586 197
Size: 1105 3250 34 3440
Fragments arranged by size:
3440 3250 1105 34
KasI G'GCGC_C
Cuts at: 5259 5507 5259
Size: 248 7581
KpnI G_GTAC'C
Cuts at: 4701 4849 4701
Size: 148 7681
MfeI C'AATT_G
Cuts at: 664 1920 4408 664
Size: 1256 2488 4085
Fragments arranged by size:
4085 2488 1256
MluI A'CGCG_T
Cuts at: 7262 7262
Size: 7829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2829 4334 4411 6500 6684 2829
Size: 1505 77 2089 184 3974
Fragments arranged by size:
3974 2089 1505 184 77
MscI TGG'CCA
Cuts at: 4515 5590 4515
Size: 1075 6754
MslI CAynn'nnrTG
Cuts at: 395 3971 4715 5818 5945 395
Size: 3576 744 1103 127 2279
Fragments arranged by size:
3576 2279 1103 744 127
MspA1I CmG'CkG
Cuts at: 277 4940 5254 5614 6627 6872 277
Size: 4663 314 360 1013 245 1234
Fragments arranged by size:
4663 1234 1013 360 314 245
NaeI GCC'GGC
Cuts at: 6010 6010
Size: 7829
NarI GG'CG_CC
Cuts at: 5260 5508 5260
Size: 248 7581
NcoI C'CATG_G
Cuts at: 2864 2864
Size: 7829
NgoMIV G'CCGG_C
Cuts at: 6008 6008
Size: 7829
NheI G'CTAG_C
Cuts at: 2280 2280
Size: 7829
NsiI A_TGCA'T
Cuts at: 1085 2903 3132 1085
Size: 1818 229 5782
Fragments arranged by size:
5782 1818 229
NspI r_CATG'y
Cuts at: 2633 4351 4882 5382 6289 2633
Size: 1718 531 500 907 4173
Fragments arranged by size:
4173 1718 907 531 500
PciI A'CATG_T
Cuts at: 4347 6285 4347
Size: 1938 5891
PfoI T'CCnGG_A
Cuts at: 434 4247 434
Size: 3813 4016
PmeI GTTT'AAAC
Cuts at: 4828 4828
Size: 7829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6968 7000 6968
Size: 32 7797
PsiI TTA'TAA
Cuts at: 3326 3326
Size: 7829
PstI C_TGCA'G
Cuts at: 276 1725 3165 4383 4876 5561 276
Size: 1449 1440 1218 493 685 2544
Fragments arranged by size:
2544 1449 1440 1218 685 493
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7829
PvuII CAG'CTG
Cuts at: 277 5254 5614 277
Size: 4977 360 2492
Fragments arranged by size:
4977 2492 360
RsrII CG'GwC_CG
Cuts at: 6024 6024
Size: 7829
SacI G_AGCT'C
Cuts at: 1464 1665 2399 4843 1464
Size: 201 734 2444 4450
Fragments arranged by size:
4450 2444 734 201
SalI G'TCGA_C
Cuts at: 4866 4866
Size: 7829
SapI GCTCTTCn'nnn_
Cuts at: 581 5852 6062 581
Size: 5271 210 2348
Fragments arranged by size:
5271 2348 210
SbfI CC_TGCA'GG
Cuts at: 4876 4876
Size: 7829
ScaI AGT'ACT
Cuts at: 734 7346 734
Size: 6612 1217
SfcI C'TryA_G
Cuts at: 33 272 1721 2078 2245 2885 3161 4379 4872
Size: 239 1449 357 167 640 276 1218 493
Cuts at: 4872 5557 6550 6741 33
Size: 685 993 191 1121
Fragments arranged by size:
1449 1218 1121 993 685 640 493 357
276 239 191 167
SfoI GGC'GCC
Cuts at: 5261 5509 5261
Size: 248 7581
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7829
SmaI CCC'GGG
Cuts at: 4851 4851
Size: 7829
SmlI C'TyrA_G
Cuts at: 1399 1465 1868 1950 2533 3725 4446 4984 5899
Size: 66 403 82 583 1192 721 538 915
Cuts at: 5899 6391 6653 1399
Size: 492 262 2575
Fragments arranged by size:
2575 1192 915 721 583 538 492 403
262 82 66
SnaBI TAC'GTA
Cuts at: 4821 4821
Size: 7829
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7829
SphI G_CATG'C
Cuts at: 4882 5382 4882
Size: 500 7329
SspI AAT'ATT
Cuts at: 2230 2230
Size: 7829
StuI AGG'CCT
Cuts at: 799 947 4546 799
Size: 148 3599 4082
Fragments arranged by size:
4082 3599 148
StyI C'CwwG_G
Cuts at: 2058 2406 2469 2864 3639 4362 4963 5317 2058
Size: 348 63 395 775 723 601 354 4570
Fragments arranged by size:
4570 775 723 601 395 354 348 63
SwaI ATTT'AAAT
Cuts at: 3462 3462
Size: 7829
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1516 2131 5412 5545 6004 6213 1516
Size: 615 3281 133 459 209 3132
Fragments arranged by size:
3281 3132 615 459 209 133
TatI w'GTAC_w
Cuts at: 634 732 831 2984 3827 4278 7344 634
Size: 98 99 2153 843 451 3066 1119
Fragments arranged by size:
3066 2153 1119 843 451 99 98
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7346 7378 7346
Size: 32 7797
Tth111I GACn'n_nGTC
Cuts at: 5626 5626
Size: 7829
XbaI T'CTAG_A
Cuts at: 4860 4860
Size: 7829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4334 5324 4334
Size: 990 6839
XhoI C'TCGA_G
Cuts at: 3725 3725
Size: 7829
XmaI C'CCGG_G
Cuts at: 4849 4849
Size: 7829
XmnI GAAnn'nnTTC
Cuts at: 590 1100 1241 1358 1984 3050 3497 3861 590
Size: 510 141 117 626 1066 447 364 4558
Fragments arranged by size:
4558 1066 626 510 447 364 141 117
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PfoI PmeI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AleI ApaI AscI BcgI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI NdeI NotI NruI PacI PasI PflMI
PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SrfI ZraI