Restriction map for
pF1KSDA0326
(Circular) (Six-base) MAPSORT of: pF1KSDA0326.seq Check: 5016 from: 1 to: 5744
pF1KSDA0326 5744 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 670 1238 670
Size: 568 5176
AccI GT'mk_AC
Cuts at: 2782 2782
Size: 5744
Acc65I G'GTAC_C
Cuts at: 2760 2760
Size: 5744
AclI AA'CG_TT
Cuts at: 5584 5584
Size: 5744
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 753 1341 1593 2058 3569 4001 4748 5016
Size: 168 588 252 465 1511 432 747 268
Cuts at: 5016 585
Size: 1313
Fragments arranged by size:
1511 1313 747 588 465 432 268 252
168
AfeI AGC'GCT
Cuts at: 1018 1018
Size: 5744
AflII C'TTAA_G
Cuts at: 2047 2250 2047
Size: 203 5541
AflIII A'CryG_T
Cuts at: 3096 4200 5177 3096
Size: 1104 977 3663
Fragments arranged by size:
3663 1104 977
AgeI A'CCGG_T
Cuts at: 1483 4132 1483
Size: 2649 3095
AleI CACnn'nnGTG
Cuts at: 197 1484 2720 197
Size: 1287 1236 3221
Fragments arranged by size:
3221 1287 1236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1894 1894
Size: 5744
AlwNI CAG_nnn'CTG
Cuts at: 3049 4616 3049
Size: 1567 4177
ApaI G_GGCC'C
Cuts at: 154 1764 2240 154
Size: 1610 476 3658
Fragments arranged by size:
3658 1610 476
ApaLI G'TGCA_C
Cuts at: 831 4514 831
Size: 3683 2061
ApoI r'AATT_y
Cuts at: 1995 2748 5097 5353 1995
Size: 753 2349 256 2386
Fragments arranged by size:
2386 2349 753 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5744
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5744
AvaI C'yCGr_G
Cuts at: 133 1003 1591 1786 2005 2518 2659 2764 133
Size: 870 588 195 219 513 141 105 3113
Fragments arranged by size:
3113 870 588 513 219 195 141 105
AvrII C'CTAG_G
Cuts at: 2220 2220
Size: 5744
BamHI G'GATC_C
Cuts at: 1892 2769 1892
Size: 877 4867
BanI G'GyrC_C
Cuts at: 199 626 1298 2480 2760 3174 3422 3457 199
Size: 427 672 1182 280 414 248 35 2486
Fragments arranged by size:
2486 1182 672 427 414 280 248 35
BanII G_rGCy'C
Cuts at: 134 154 181 229 524 697 781 1117 1453
Size: 20 27 48 295 173 84 336 336
Cuts at: 1453 1621 1764 1787 2240 2350 2380 2410 2758
Size: 168 143 23 453 110 30 30 348
Cuts at: 2758 3788 134
Size: 1030 2090
Fragments arranged by size:
2090 1030 453 348 336 336 295 173
168 143 110 84 48 30 30 27
23 20
BbeI G_GCGC'C
Cuts at: 3178 3426 3178
Size: 248 5496
BbsI GAAGACnn'nnnn_
Cuts at: 1750 1750
Size: 5744
BbvCI CC'TCA_GC
Cuts at: 2299 2299
Size: 5744
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1170 1204 1170
Size: 34 5710
BciVI GTATCCnnnnn_n'
Cuts at: 3642 4403 5744 3642
Size: 761 1341 3642
Fragments arranged by size:
3642 1341 761
BclI T'GATC_A
Cuts at: 3264 3264
Size: 5744
BglI GCCn_nnn'nGGC
Cuts at: 457 457
Size: 5744
BglII A'GATC_T
Cuts at: 3259 5296 3259
Size: 2037 3707
BlpI GC'TnA_GC
Cuts at: 2856 2856
Size: 5744
Bme1580I G_kGCm'C
Cuts at: 154 751 835 1003 1255 1339 1591 1764 2240
Size: 597 84 168 252 84 252 173 476
Cuts at: 2240 2659 3369 3462 4518 154
Size: 419 710 93 1056 1380
Fragments arranged by size:
1380 1056 710 597 476 419 252 252
173 168 93 84 84
BmgBI CAC'GTC
Cuts at: 1638 1638
Size: 5744
BmrI ACTGGGnnnn_n'
Cuts at: 373 496 1249 2956 3037 3372 5516 373
Size: 123 753 1707 81 335 2144 601
Fragments arranged by size:
2144 1707 753 601 335 123 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 339 4111 339
Size: 3772 1972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 795 1732 2143 2413 2516 334
Size: 461 937 411 270 103 3562
Fragments arranged by size:
3562 937 461 411 270 103
Bpu10I CC'TnA_GC
Cuts at: 1279 2299 1279
Size: 1020 4724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2920 3799 4291 4589 1027
Size: 1893 879 492 298 2182
Fragments arranged by size:
2182 1893 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 260 2569 5448 260
Size: 2309 2879 556
Fragments arranged by size:
2879 2309 556
BsaAI yAC'GTr
Cuts at: 2736 3097 2736
Size: 361 5383
BsaBI GATnn'nnATC
Cuts at: 6 1897 3258 5295 6
Size: 1891 1361 2037 455
Fragments arranged by size:
2037 1891 1361 455
BsaHI Gr'CG_yC
Cuts at: 3175 3423 4125 5621 3175
Size: 248 702 1496 3298
Fragments arranged by size:
3298 1496 702 248
BsaWI w'CCGG_w
Cuts at: 168 1483 2933 3454 4132 4406 4553 4964 168
Size: 1315 1450 521 678 274 147 411 948
Fragments arranged by size:
1450 1315 948 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 2101 2131 649
Size: 30 1422 30 4262
Fragments arranged by size:
4262 1422 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 265 1030 1949 2146 235
Size: 30 765 919 197 3833
Fragments arranged by size:
3833 919 765 197 30
BseYI C'CCAG_C
Cuts at: 408 523 1127 1749 1779 2239 2367 3169 3927
Size: 115 604 622 30 460 128 802 758
Cuts at: 3927 4504 5000 408
Size: 577 496 1152
Fragments arranged by size:
1152 802 758 622 604 577 496 460
128 115 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 727 811 1068 2302 562
Size: 165 84 257 1234 4004
Fragments arranged by size:
4004 1234 257 165 84
BsiEI CG_ry'CG
Cuts at: 104 2463 3286 3332 4540 104
Size: 2359 823 46 1208 1308
Fragments arranged by size:
2359 1308 1208 823 46
BsiHKAI G_wGCw'C
Cuts at: 697 781 835 865 1117 1453 1621 1675 2156
Size: 84 54 30 252 336 168 54 481
Cuts at: 2156 2350 2380 2758 3536 3726 4518 697
Size: 194 30 378 778 190 792 1923
Fragments arranged by size:
1923 792 778 481 378 336 252 194
190 168 84 54 54 30 30
BsmI GAATG_Cn'
Cuts at: 2284 2284
Size: 5744
Bsp1286I G_dGCh'C
Cuts at: 134 154 181 229 524 697 751 781 835
Size: 20 27 48 295 173 54 30 54
Cuts at: 835 865 1003 1117 1255 1339 1453 1591 1621
Size: 30 138 114 138 84 114 138 30
Cuts at: 1621 1675 1764 1787 2156 2240 2350 2380 2410
Size: 54 89 23 369 84 110 30 30
Cuts at: 2410 2659 2758 3369 3462 3536 3726 3788 4518
Size: 249 99 611 93 74 190 62 730
Cuts at: 4518 134
Size: 1360
Fragments arranged by size:
1360 730 611 369 295 249 190 173
138 138 138 114 114 110 99 93
89 84 84 74 62 54 54 54
48 30 30 30 30 30 27 23
20
BspEI T'CCGG_A
Cuts at: 168 4964 168
Size: 4796 948
BspHI T'CATG_A
Cuts at: 1445 4920 1445
Size: 3475 2269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 670 1238 2794 3008 3310 3691 670
Size: 568 1556 214 302 381 2723
Fragments arranged by size:
2723 1556 568 381 302 214
BsrBI CCG'CTC
Cuts at: 50 651 1323 1407 2026 4036 4090 5574 5739
Size: 601 672 84 619 2010 54 1484 165
Cuts at: 5739 50
Size: 55
Fragments arranged by size:
2010 1484 672 619 601 165 84 55
54
BsrDI GCAATG_nn'
Cuts at: 3656 3656
Size: 5744
BsrFI r'CCGG_y
Cuts at: 727 895 1483 1567 2671 3742 3923 4132 727
Size: 168 588 84 1104 1071 181 209 2339
Fragments arranged by size:
2339 1104 1071 588 209 181 168 84
BssSI C'ACGA_G
Cuts at: 1705 1840 4015 4373 1705
Size: 135 2175 358 3076
Fragments arranged by size:
3076 2175 358 135
BstAPI GCAn_nnn'nTGC
Cuts at: 587 705 839 1091 3121 587
Size: 118 134 252 2030 3210
Fragments arranged by size:
3210 2030 252 134 118
BstBI TT'CG_AA
Cuts at: 110 4105 110
Size: 3995 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 381 440 381
Size: 59 5685
BstYI r'GATC_y
Cuts at: 1 156 319 392 1055 1892 2769 3236 3259
Size: 155 163 73 663 837 877 467 23
Cuts at: 3259 3594 3840 4852 4938 4950 5296 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 877 837 663 467 449 346 335
246 163 155 86 73 23 12
BtgI C'CryG_G
Cuts at: 125 1398 2175 2604 125
Size: 1273 777 429 3265
Fragments arranged by size:
3265 1273 777 429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1920 3694 3875 4038 1920
Size: 1774 181 163 3626
Fragments arranged by size:
3626 1774 181 163
BtsI GCAGTG_nn'
Cuts at: 475 2121 2987 475
Size: 1646 866 3232
Fragments arranged by size:
3232 1646 866
DraI TTT'AAA
Cuts at: 2268 2743 2268
Size: 475 5269
DraIII CAC_nnn'GTG
Cuts at: 705 839 1091 1725 705
Size: 134 252 634 4724
Fragments arranged by size:
4724 634 252 134
DrdI GACnn_nn'nnGTC
Cuts at: 337 3450 4308 337
Size: 3113 858 1773
Fragments arranged by size:
3113 1773 858
EaeI y'GGCC_r
Cuts at: 1023 1191 3329 3503 3894 3921 5266 1023
Size: 168 2138 174 391 27 1345 1501
Fragments arranged by size:
2138 1501 1345 391 174 168 27
EagI C'GGCC_G
Cuts at: 3329 3329
Size: 5744
EarI CTCTTCn'nnn_
Cuts at: 212 1093 2338 2350 2368 2385 3767 3977 212
Size: 881 1245 12 18 17 1382 210 1979
Fragments arranged by size:
1979 1382 1245 881 210 18 17 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 577 1453 3952 4262 4408 5554 577
Size: 876 2499 310 146 1146 767
Fragments arranged by size:
2499 1146 876 767 310 146
EcoICRI GAG'CTC
Cuts at: 695 779 1115 1451 1619 2348 2378 2756 695
Size: 84 336 336 168 729 30 378 3683
Fragments arranged by size:
3683 729 378 336 336 168 84 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 585 753 795 1341 1593 1732 2058 2143
Size: 251 168 42 546 252 139 326 85
Cuts at: 2143 2413 2516 3569 4001 4748 5016 334
Size: 270 103 1053 432 747 268 1062
Fragments arranged by size:
1062 1053 747 546 432 326 270 268
252 251 168 139 103 85 42
EcoNI CCTnn'n_nnAGG
Cuts at: 1234 1988 1234
Size: 754 4990
EcoO109I rG'GnC_Cy
Cuts at: 150 330 1239 1760 1791 2031 2236 2447 2523
Size: 180 909 521 31 240 205 211 76
Cuts at: 2523 2883 150
Size: 360 3011
Fragments arranged by size:
3011 909 521 360 240 211 205 180
76 31
EcoRI G'AATT_C
Cuts at: 1995 2748 1995
Size: 753 4991
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2000 3763 2000
Size: 1763 3981
FspI TGC'GCA
Cuts at: 3525 3525
Size: 5744
HaeII r_GCGC'y
Cuts at: 479 1020 1286 3178 3426 4448 5427 479
Size: 541 266 1892 248 1022 979 796
Fragments arranged by size:
1892 1022 979 796 541 266 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3586 3616 3618 3648 3586
Size: 30 2 30 5682
Fragments arranged by size:
5682 30 30 2
HincII GTy'rAC
Cuts at: 2783 2783
Size: 5744
HindIII A'AGCT_T
Cuts at: 2289 2289
Size: 5744
KasI G'GCGC_C
Cuts at: 3174 3422 3174
Size: 248 5496
KpnI G_GTAC'C
Cuts at: 2764 2764
Size: 5744
MluI A'CGCG_T
Cuts at: 5177 5177
Size: 5744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 806 1394 1532 1646 1700 4415 4599 806
Size: 588 138 114 54 2715 184 1951
Fragments arranged by size:
2715 1951 588 184 138 114 54
MscI TGG'CCA
Cuts at: 3505 3505
Size: 5744
MslI CAynn'nnrTG
Cuts at: 197 1484 1648 1876 2720 3733 3860 197
Size: 1287 164 228 844 1013 127 2081
Fragments arranged by size:
2081 1287 1013 844 228 164 127
MspA1I CmG'CkG
Cuts at: 464 773 1082 1221 1400 2432 2855 3169 3529
Size: 309 309 139 179 1032 423 314 360
Cuts at: 3529 4542 4787 464
Size: 1013 245 1421
Fragments arranged by size:
1421 1032 1013 423 360 314 309 309
245 179 139
NaeI GCC'GGC
Cuts at: 2673 3925 2673
Size: 1252 4492
NarI GG'CG_CC
Cuts at: 3175 3423 3175
Size: 248 5496
NcoI C'CATG_G
Cuts at: 125 2604 125
Size: 2479 3265
NgoMIV G'CCGG_C
Cuts at: 2671 3923 2671
Size: 1252 4492
NspI r_CATG'y
Cuts at: 2797 3297 4204 2797
Size: 500 907 4337
Fragments arranged by size:
4337 907 500
PasI CC'CwG_GG
Cuts at: 1764 2412 1764
Size: 648 5096
PciI A'CATG_T
Cuts at: 4200 4200
Size: 5744
PflMI CCAn_nnn'nTGG
Cuts at: 1710 1710
Size: 5744
PfoI T'CCnGG_A
Cuts at: 1694 2138 2390 1694
Size: 444 252 5048
Fragments arranged by size:
5048 444 252
PmeI GTTT'AAAC
Cuts at: 2743 2743
Size: 5744
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1228 1260 1648 1680 4883 4915 1228
Size: 32 388 32 3203 32 2057
Fragments arranged by size:
3203 2057 388 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 330 1239 1791 2523 330
Size: 909 552 732 3551
Fragments arranged by size:
3551 909 732 552
PspOMI G'GGCC_C
Cuts at: 150 1760 2236 150
Size: 1610 476 3658
Fragments arranged by size:
3658 1610 476
PstI C_TGCA'G
Cuts at: 164 2791 3476 164
Size: 2627 685 2432
Fragments arranged by size:
2627 2432 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5744
PvuII CAG'CTG
Cuts at: 464 773 3169 3529 464
Size: 309 2396 360 2679
Fragments arranged by size:
2679 2396 360 309
RsrII CG'GwC_CG
Cuts at: 3939 3939
Size: 5744
SacI G_AGCT'C
Cuts at: 697 781 1117 1453 1621 2350 2380 2758 697
Size: 84 336 336 168 729 30 378 3683
Fragments arranged by size:
3683 729 378 336 336 168 84 30
SacII CC_GC'GG
Cuts at: 1401 1401
Size: 5744
SalI G'TCGA_C
Cuts at: 2781 2781
Size: 5744
SanDI GG'GwC_CC
Cuts at: 1791 1791
Size: 5744
SapI GCTCTTCn'nnn_
Cuts at: 1093 2338 2368 2385 3767 3977 1093
Size: 1245 30 17 1382 210 2860
Fragments arranged by size:
2860 1382 1245 210 30 17
SbfI CC_TGCA'GG
Cuts at: 2791 2791
Size: 5744
ScaI AGT'ACT
Cuts at: 5261 5261
Size: 5744
SexAI A'CCwGG_T
Cuts at: 701 785 701
Size: 84 5660
SfcI C'TryA_G
Cuts at: 33 160 2787 3472 4465 4656 33
Size: 127 2627 685 993 191 1121
Fragments arranged by size:
2627 1121 993 685 191 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 457 457
Size: 5744
SfoI GGC'GCC
Cuts at: 3176 3424 3176
Size: 248 5496
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5744
SgrAI Cr'CCGG_yG
Cuts at: 727 895 1483 1567 727
Size: 168 588 84 4904
Fragments arranged by size:
4904 588 168 84
SmaI CCC'GGG
Cuts at: 2007 2766 2007
Size: 759 4985
SmlI C'TyrA_G
Cuts at: 1042 2047 2250 2899 3814 4306 4568 1042
Size: 1005 203 649 915 492 262 2218
Fragments arranged by size:
2218 1005 915 649 492 262 203
SnaBI TAC'GTA
Cuts at: 2736 2736
Size: 5744
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5744
SphI G_CATG'C
Cuts at: 2797 3297 2797
Size: 500 5244
StuI AGG'CCT
Cuts at: 451 1690 451
Size: 1239 4505
StyI C'CwwG_G
Cuts at: 125 290 380 446 1975 2220 2604 2878 3232
Size: 165 90 66 1529 245 384 274 354
Cuts at: 3232 125
Size: 2637
Fragments arranged by size:
2637 1529 384 354 274 245 165 90
66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 871 988 2188 2512 2732 3327 3460 3919 4128
Size: 117 1200 324 220 595 133 459 209
Cuts at: 4128 871
Size: 2487
Fragments arranged by size:
2487 1200 595 459 324 220 209 133
117
TatI w'GTAC_w
Cuts at: 5259 5259
Size: 5744
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 871 903 5261 5293 871
Size: 32 4358 32 1322
Fragments arranged by size:
4358 1322 32 32
Tth111I GACn'n_nGTC
Cuts at: 3541 3541
Size: 5744
XbaI T'CTAG_A
Cuts at: 2775 2775
Size: 5744
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2631 3239 2631
Size: 608 5136
XmaI C'CCGG_G
Cuts at: 2005 2764 2005
Size: 759 4985
XmnI GAAnn'nnTTC
Cuts at: 850 1018 1102 1663 1690 850
Size: 168 84 561 27 4904
Fragments arranged by size:
4904 561 168 84 27
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AloI AscI BaeI BfrBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SrfI SspI SwaI XhoI ZraI