Restriction map for
pF1KSDA0316
(Circular) (Six-base) MAPSORT of: pF1KSDA0316.seq Check: 4415 from: 1 to: 7103
pF1KSDA0316 7103 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3652 3652
Size: 7103
AccI GT'mk_AC
Cuts at: 1785 4141 1785
Size: 2356 4747
Acc65I G'GTAC_C
Cuts at: 1305 4119 1305
Size: 2814 4289
AclI AA'CG_TT
Cuts at: 6943 6943
Size: 7103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 167 476 1604 2062 3437 3584 3758 3890
Size: 47 309 1128 458 1375 147 174 132
Cuts at: 3890 4928 5360 6107 6375 120
Size: 1038 432 747 268 848
Fragments arranged by size:
1375 1128 1038 848 747 458 432 309
268 174 147 132 47
AfeI AGC'GCT
Cuts at: 1426 1426
Size: 7103
AflIII A'CryG_T
Cuts at: 4455 5559 6536 4455
Size: 1104 977 5022
Fragments arranged by size:
5022 1104 977
AgeI A'CCGG_T
Cuts at: 504 5491 504
Size: 4987 2116
AleI CACnn'nnGTG
Cuts at: 3826 3826
Size: 7103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2659 3207 2659
Size: 548 6555
AlwNI CAG_nnn'CTG
Cuts at: 551 1738 1953 3815 4000 4408 5975 551
Size: 1187 215 1862 185 408 1567 1679
Fragments arranged by size:
1862 1679 1567 1187 408 215 185
ApaI G_GGCC'C
Cuts at: 1454 1454
Size: 7103
ApaLI G'TGCA_C
Cuts at: 5873 5873
Size: 7103
ApoI r'AATT_y
Cuts at: 2262 3316 4107 6456 6712 2262
Size: 1054 791 2349 256 2653
Fragments arranged by size:
2653 2349 1054 791 256
AseI AT'TA_AT
Cuts at: 20 494 20
Size: 474 6629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7103
AvaI C'yCGr_G
Cuts at: 201 471 1963 2617 2844 4123 201
Size: 270 1492 654 227 1279 3181
Fragments arranged by size:
3181 1492 1279 654 270 227
BamHI G'GATC_C
Cuts at: 4128 4128
Size: 7103
BanI G'GyrC_C
Cuts at: 225 1305 3237 4119 4533 4781 4816 225
Size: 1080 1932 882 414 248 35 2512
Fragments arranged by size:
2512 1932 1080 882 414 248 35
BanII G_rGCy'C
Cuts at: 1454 2751 3525 3763 3921 4117 5147 1454
Size: 1297 774 238 158 196 1030 3410
Fragments arranged by size:
3410 1297 1030 774 238 196 158
BbeI G_GCGC'C
Cuts at: 4537 4785 4537
Size: 248 6855
BbsI GAAGACnn'nnnn_
Cuts at: 126 173 1864 2825 3323 126
Size: 47 1691 961 498 3906
Fragments arranged by size:
3906 1691 961 498 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 143 177 143
Size: 34 7069
BciVI GTATCCnnnnn_n'
Cuts at: 1648 5001 5762 7103 1648
Size: 3353 761 1341 1648
Fragments arranged by size:
3353 1648 1341 761
BclI T'GATC_A
Cuts at: 4623 4623
Size: 7103
BglII A'GATC_T
Cuts at: 4618 6655 4618
Size: 2037 5066
BlpI GC'TnA_GC
Cuts at: 1954 2003 4215 1954
Size: 49 2212 4842
Fragments arranged by size:
4842 2212 49
Bme1580I G_kGCm'C
Cuts at: 1454 2687 4728 4821 5877 1454
Size: 1233 2041 93 1056 2680
Fragments arranged by size:
2680 2041 1233 1056 93
BmgBI CAC'GTC
Cuts at: 1524 3585 1524
Size: 2061 5042
BmrI ACTGGGnnnn_n'
Cuts at: 4315 4396 4731 6875 4315
Size: 81 335 2144 4543
Fragments arranged by size:
4543 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 852 5470 852
Size: 4618 2485
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 2191 2216 2474 3388 892
Size: 1299 25 258 914 4607
Fragments arranged by size:
4607 1299 914 258 25
Bpu10I CC'TnA_GC
Cuts at: 3352 3549 3352
Size: 197 6906
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 1339 4279 5158 5650 5948 237
Size: 1102 2940 879 492 298 1392
Fragments arranged by size:
2940 1392 1102 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6807 6807
Size: 7103
BsaAI yAC'GTr
Cuts at: 1581 4095 4456 1581
Size: 2514 361 4228
Fragments arranged by size:
4228 2514 361
BsaBI GATnn'nnATC
Cuts at: 6 4617 6654 6
Size: 4611 2037 455
Fragments arranged by size:
4611 2037 455
BsaHI Gr'CG_yC
Cuts at: 4534 4782 5484 6980 4534
Size: 248 702 1496 4657
Fragments arranged by size:
4657 1496 702 248
BsaWI w'CCGG_w
Cuts at: 504 4292 4813 5491 5765 5912 6323 504
Size: 3788 521 678 274 147 411 1284
Fragments arranged by size:
3788 1284 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2060 2090 3124 3154 2060
Size: 30 1034 30 6009
Fragments arranged by size:
6009 1034 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 341 559 685 1564 1567 2081 2410 3313 3316
Size: 218 126 879 3 514 329 903 3
Cuts at: 3316 3925 341
Size: 609 3519
Fragments arranged by size:
3519 903 879 609 514 329 218 126
3 3
BseYI C'CCAG_C
Cuts at: 384 952 2742 3004 4528 5286 5863 6359 384
Size: 568 1790 262 1524 758 577 496 1128
Fragments arranged by size:
1790 1524 1128 758 577 568 496 262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 1313 1432 3151 3177 3943 3991 500
Size: 813 119 1719 26 766 48 3612
Fragments arranged by size:
3612 1719 813 766 119 48 26
BsiEI CG_ry'CG
Cuts at: 104 4645 4691 5899 104
Size: 4541 46 1208 1308
Fragments arranged by size:
4541 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1901 2751 3358 3525 3763 4117 4895 5085 5877
Size: 850 607 167 238 354 778 190 792
Cuts at: 5877 1901
Size: 3127
Fragments arranged by size:
3127 850 792 778 607 354 238 190
167
BsmBI CGTCTCn'nnnn_
Cuts at: 2739 2739
Size: 7103
Bsp1286I G_dGCh'C
Cuts at: 1454 1901 2687 2751 3358 3525 3763 3921 4117
Size: 447 786 64 607 167 238 158 196
Cuts at: 4117 4728 4821 4895 5085 5147 5877 1454
Size: 611 93 74 190 62 730 2680
Fragments arranged by size:
2680 786 730 611 607 447 238 196
190 167 158 93 74 64 62
BspEI T'CCGG_A
Cuts at: 6323 6323
Size: 7103
BspHI T'CATG_A
Cuts at: 915 6279 915
Size: 5364 1739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3652 4153 4367 4669 5050 3652
Size: 501 214 302 381 5705
Fragments arranged by size:
5705 501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 528 3840 5395 5449 6933 7098 50
Size: 478 3312 1555 54 1484 165 55
Fragments arranged by size:
3312 1555 1484 478 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1142 2543 2856 5015 1142
Size: 1401 313 2159 3230
Fragments arranged by size:
3230 2159 1401 313
BsrFI r'CCGG_y
Cuts at: 504 1661 1901 2427 5101 5282 5491 504
Size: 1157 240 526 2674 181 209 2116
Fragments arranged by size:
2674 2116 1157 526 240 209 181
BsrGI T'GTAC_A
Cuts at: 1139 1910 1139
Size: 771 6332
BssSI C'ACGA_G
Cuts at: 2404 3335 5374 5732 2404
Size: 931 2039 358 3775
Fragments arranged by size:
3775 2039 931 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4480 4480
Size: 7103
BstBI TT'CG_AA
Cuts at: 110 5464 110
Size: 5354 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 1497 1635 3828 1497
Size: 138 2193 4772
Fragments arranged by size:
4772 2193 138
BstYI r'GATC_y
Cuts at: 1 1002 1026 2223 2657 4128 4595 4618 4953
Size: 1001 24 1197 434 1471 467 23 335
Cuts at: 4953 5199 6211 6297 6309 6655 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1471 1197 1012 1001 467 449 434 346
335 246 86 24 23 12
BstZ17I GTA'TAC
Cuts at: 1786 1786
Size: 7103
Bsu36I CC'TnA_GG
Cuts at: 3668 3668
Size: 7103
BtgI C'CryG_G
Cuts at: 125 2306 3827 3936 3985 125
Size: 2181 1521 109 49 3243
Fragments arranged by size:
3243 2181 1521 109 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2300 2471 5053 5234 5397 2300
Size: 171 2582 181 163 4006
Fragments arranged by size:
4006 2582 181 171 163
BtsI GCAGTG_nn'
Cuts at: 414 512 2205 3727 3810 3816 4346 414
Size: 98 1693 1522 83 6 530 3171
Fragments arranged by size:
3171 1693 1522 530 98 83 6
ClaI AT'CG_AT
Cuts at: 536 562 536
Size: 26 7077
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1788 1823 1788
Size: 35 7068
DraI TTT'AAA
Cuts at: 4102 4102
Size: 7103
DraIII CAC_nnn'GTG
Cuts at: 445 1886 445
Size: 1441 5662
DrdI GACnn_nn'nnGTC
Cuts at: 4809 5667 4809
Size: 858 6245
EaeI y'GGCC_r
Cuts at: 190 687 1124 1700 2429 2540 2891 2936 3218
Size: 497 437 576 729 111 351 45 282
Cuts at: 3218 3410 3710 4688 4862 5253 5280 6625 190
Size: 192 300 978 174 391 27 1345 668
Fragments arranged by size:
1345 978 729 668 576 497 437 391
351 300 282 192 174 111 45 27
EagI C'GGCC_G
Cuts at: 4688 4688
Size: 7103
EarI CTCTTCn'nnn_
Cuts at: 2287 2579 2868 2963 3777 5126 5336 2287
Size: 292 289 95 814 1349 210 4054
Fragments arranged by size:
4054 1349 814 292 289 210 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5311 5621 5767 6913 5311
Size: 310 146 1146 5501
Fragments arranged by size:
5501 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2749 3523 3761 4115 2749
Size: 774 238 354 5737
Fragments arranged by size:
5737 774 354 238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 120 167 476 892 1604 2062 2191 2216 2474
Size: 47 309 416 712 458 129 25 258
Cuts at: 2474 3388 3437 3584 3758 3890 4928 5360 6107
Size: 914 49 147 174 132 1038 432 747
Cuts at: 6107 6375 120
Size: 268 848
Fragments arranged by size:
1038 914 848 747 712 458 432 416
309 268 258 174 147 132 129 49
47 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 2492
Size: 7103
EcoO109I rG'GnC_Cy
Cuts at: 375 1451 1735 1971 2158 2217 2400 4242 375
Size: 1076 284 236 187 59 183 1842 3236
Fragments arranged by size:
3236 1842 1076 284 236 187 183 59
EcoRI G'AATT_C
Cuts at: 4107 4107
Size: 7103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 924 5122 924
Size: 4198 2905
FspI TGC'GCA
Cuts at: 4884 4884
Size: 7103
HaeII r_GCGC'y
Cuts at: 1428 2696 4537 4785 5807 6786 1428
Size: 1268 1841 248 1022 979 1745
Fragments arranged by size:
1841 1745 1268 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 515 547 918 950 957 989 2461 2493 2746
Size: 32 371 32 7 32 1472 32 253
Cuts at: 2746 2778 2821 2853 3124 3156 3619 3651 4945
Size: 32 43 32 271 32 463 32 1294
Cuts at: 4945 4975 4977 5007 515
Size: 30 2 30 2611
Fragments arranged by size:
2611 1472 1294 463 371 271 253 43
32 32 32 32 32 32 32 32
30 30 7 2
HincII GTy'rAC
Cuts at: 1527 3346 4142 1527
Size: 1819 796 4488
Fragments arranged by size:
4488 1819 796
HindIII A'AGCT_T
Cuts at: 157 3661 157
Size: 3504 3599
KasI G'GCGC_C
Cuts at: 4533 4781 4533
Size: 248 6855
KpnI G_GTAC'C
Cuts at: 1309 4123 1309
Size: 2814 4289
MfeI C'AATT_G
Cuts at: 1007 1007
Size: 7103
MluI A'CGCG_T
Cuts at: 6536 6536
Size: 7103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 677 5774 5958 677
Size: 5097 184 1822
Fragments arranged by size:
5097 1822 184
MscI TGG'CCA
Cuts at: 1702 2542 2893 2938 3412 3712 4864 1702
Size: 840 351 45 474 300 1152 3941
Fragments arranged by size:
3941 1152 840 474 351 300 45
MslI CAynn'nnrTG
Cuts at: 3112 3826 5092 5219 3112
Size: 714 1266 127 4996
Fragments arranged by size:
4996 1266 714 127
MspA1I CmG'CkG
Cuts at: 1953 2007 2308 3004 4214 4528 4888 5901 6146
Size: 54 301 696 1210 314 360 1013 245
Cuts at: 6146 1953
Size: 2910
Fragments arranged by size:
2910 1210 1013 696 360 314 301 245
54
NaeI GCC'GGC
Cuts at: 2429 5284 2429
Size: 2855 4248
NarI GG'CG_CC
Cuts at: 4534 4782 4534
Size: 248 6855
NcoI C'CATG_G
Cuts at: 125 125
Size: 7103
NgoMIV G'CCGG_C
Cuts at: 2427 5282 2427
Size: 2855 4248
NspI r_CATG'y
Cuts at: 2931 4156 4656 5563 2931
Size: 1225 500 907 4471
Fragments arranged by size:
4471 1225 907 500
PasI CC'CwG_GG
Cuts at: 3852 3922 3852
Size: 70 7033
PciI A'CATG_T
Cuts at: 5559 5559
Size: 7103
PflMI CCAn_nnn'nTGG
Cuts at: 2131 2256 2131
Size: 125 6978
PfoI T'CCnGG_A
Cuts at: 1678 1678
Size: 7103
PmeI GTTT'AAAC
Cuts at: 4102 4102
Size: 7103
PmlI CAC'GTG
Cuts at: 1581 1581
Size: 7103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6242 6274 6242
Size: 32 7071
PpuMI rG'GwC_Cy
Cuts at: 375 1735 2158 2400 375
Size: 1360 423 242 5078
Fragments arranged by size:
5078 1360 423 242
PspOMI G'GGCC_C
Cuts at: 1450 1450
Size: 7103
PstI C_TGCA'G
Cuts at: 793 3758 4150 4835 793
Size: 2965 392 685 3061
Fragments arranged by size:
3061 2965 685 392
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7103
PvuII CAG'CTG
Cuts at: 1953 4528 4888 1953
Size: 2575 360 4168
Fragments arranged by size:
4168 2575 360
RsrII CG'GwC_CG
Cuts at: 5298 5298
Size: 7103
SacI G_AGCT'C
Cuts at: 2751 3525 3763 4117 2751
Size: 774 238 354 5737
Fragments arranged by size:
5737 774 354 238
SacII CC_GC'GG
Cuts at: 2309 2309
Size: 7103
SalI G'TCGA_C
Cuts at: 4140 4140
Size: 7103
SanDI GG'GwC_CC
Cuts at: 375 375
Size: 7103
SapI GCTCTTCn'nnn_
Cuts at: 2287 2963 3777 5126 5336 2287
Size: 676 814 1349 210 4054
Fragments arranged by size:
4054 1349 814 676 210
SbfI CC_TGCA'GG
Cuts at: 4150 4150
Size: 7103
ScaI AGT'ACT
Cuts at: 6620 6620
Size: 7103
SexAI A'CCwGG_T
Cuts at: 441 441
Size: 7103
SfcI C'TryA_G
Cuts at: 33 789 1926 1989 3754 4146 4831 5824 6015
Size: 756 1137 63 1765 392 685 993 191
Cuts at: 6015 33
Size: 1121
Fragments arranged by size:
1765 1137 1121 993 756 685 392 191
63
SfoI GGC'GCC
Cuts at: 4535 4783 4535
Size: 248 6855
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7103
SmaI CCC'GGG
Cuts at: 473 1965 4125 473
Size: 1492 2160 3451
Fragments arranged by size:
3451 2160 1492
SmlI C'TyrA_G
Cuts at: 216 1354 4258 5173 5665 5927 216
Size: 1138 2904 915 492 262 1392
Fragments arranged by size:
2904 1392 1138 915 492 262
SnaBI TAC'GTA
Cuts at: 4095 4095
Size: 7103
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7103
SphI G_CATG'C
Cuts at: 4156 4656 4156
Size: 500 6603
StyI C'CwwG_G
Cuts at: 125 1340 1764 2102 2130 3765 4237 4591 125
Size: 1215 424 338 28 1635 472 354 2637
Fragments arranged by size:
2637 1635 1215 472 424 354 338 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 534 2992 3132 3977 4686 4819 5278 5487 534
Size: 2458 140 845 709 133 459 209 2150
Fragments arranged by size:
2458 2150 845 709 459 209 140 133
TatI w'GTAC_w
Cuts at: 1139 1910 6618 1139
Size: 771 4708 1624
Fragments arranged by size:
4708 1624 771
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 284 316 1872 1904 2060 2092 6620 6652 284
Size: 32 1556 32 156 32 4528 32 735
Fragments arranged by size:
4528 1556 735 156 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 177 3138 3557 4900 177
Size: 2961 419 1343 2380
Fragments arranged by size:
2961 2380 1343 419
XbaI T'CTAG_A
Cuts at: 2751 4134 2751
Size: 1383 5720
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1491 4598 1491
Size: 3107 3996
XmaI C'CCGG_G
Cuts at: 471 1963 4123 471
Size: 1492 2160 3451
Fragments arranged by size:
3451 2160 1492
XmnI GAAnn'nnTTC
Cuts at: 2296 2296
Size: 7103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI AvrII BaeI BbvCI
BfrBI BglI BmtI BsiWI BsmI BssHII BstEII EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PshAI PsiI PspXI PsrI SfiI SgrAI SrfI
SspI StuI SwaI XhoI ZraI