Restriction map for
pF1KSDA0307
(Circular) (Six-base) MAPSORT of: pF1KSDA0307.seq Check: 7297 from: 1 to: 5257
pF1KSDA0307 5257 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1245 1245
Size: 5257
AccI GT'mk_AC
Cuts at: 2293 2293
Size: 5257
Acc65I G'GTAC_C
Cuts at: 2271 2271
Size: 5257
AclI AA'CG_TT
Cuts at: 5097 5097
Size: 5257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 526 751 1004 1399 1686 1694 2156 3080
Size: 410 225 253 395 287 8 462 924
Cuts at: 3080 3512 4261 4529 116
Size: 432 749 268 844
Fragments arranged by size:
924 844 749 462 432 410 395 287
268 253 225 8
AflIII A'CryG_T
Cuts at: 1769 2607 3713 4690 1769
Size: 838 1106 977 2336
Fragments arranged by size:
2336 1106 977 838
AgeI A'CCGG_T
Cuts at: 3645 3645
Size: 5257
AhdI GACnn_n'nnGTC
Cuts at: 1820 1820
Size: 5257
AleI CACnn'nnGTG
Cuts at: 1138 1138
Size: 5257
AlwNI CAG_nnn'CTG
Cuts at: 504 1633 2560 4129 504
Size: 1129 927 1569 1632
Fragments arranged by size:
1632 1569 1129 927
ApaLI G'TGCA_C
Cuts at: 690 1441 4027 690
Size: 751 2586 1920
Fragments arranged by size:
2586 1920 751
ApoI r'AATT_y
Cuts at: 277 1207 2259 4610 4866 277
Size: 930 1052 2351 256 668
Fragments arranged by size:
2351 1052 930 668 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5257
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5257
AvaI C'yCGr_G
Cuts at: 2275 2275
Size: 5257
BamHI G'GATC_C
Cuts at: 1143 2280 1143
Size: 1137 4120
BanI G'GyrC_C
Cuts at: 1089 2271 2685 2933 2968 1089
Size: 1182 414 248 35 3378
Fragments arranged by size:
3378 1182 414 248 35
BanII G_rGCy'C
Cuts at: 350 790 1627 2269 3299 350
Size: 440 837 642 1030 2308
Fragments arranged by size:
2308 1030 837 642 440
BbeI G_GCGC'C
Cuts at: 2689 2937 2689
Size: 248 5009
BbsI GAAGACnn'nnnn_
Cuts at: 739 995 2210 739
Size: 256 1215 3786
Fragments arranged by size:
3786 1215 256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2191 2225 2191
Size: 34 5223
BciVI GTATCCnnnnn_n'
Cuts at: 937 3153 3916 5257 937
Size: 2216 763 1341 937
Fragments arranged by size:
2216 1341 937 763
BclI T'GATC_A
Cuts at: 2775 2775
Size: 5257
BfrBI ATG'CAT
Cuts at: 1067 1067
Size: 5257
BglI GCCn_nnn'nGGC
Cuts at: 172 172
Size: 5257
BglII A'GATC_T
Cuts at: 1652 2770 4809 1652
Size: 1118 2039 2100
Fragments arranged by size:
2100 2039 1118
BlpI GC'TnA_GC
Cuts at: 1626 2367 1626
Size: 741 4516
Bme1580I G_kGCm'C
Cuts at: 694 1094 1445 2880 2973 4031 694
Size: 400 351 1435 93 1058 1920
Fragments arranged by size:
1920 1435 1058 400 351 93
BmgBI CAC'GTC
Cuts at: 666 666
Size: 5257
BmrI ACTGGGnnnn_n'
Cuts at: 266 609 746 2120 2467 2548 2883 5029 266
Size: 343 137 1374 347 81 335 2146 494
Fragments arranged by size:
2146 1374 494 347 343 335 137 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2127 3622 2127
Size: 1495 3762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1748 1926 1965 1985 1027
Size: 721 178 39 20 4299
Fragments arranged by size:
4299 721 178 39 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2431 3310 3804 4102 2431
Size: 879 494 298 3586
Fragments arranged by size:
3586 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 4961 4961
Size: 5257
BsaAI yAC'GTr
Cuts at: 2247 2608 2247
Size: 361 4896
BsaBI GATnn'nnATC
Cuts at: 6 1327 1461 2769 4808 6
Size: 1321 134 1308 2039 455
Fragments arranged by size:
2039 1321 1308 455 134
BsaHI Gr'CG_yC
Cuts at: 2686 2934 3638 5134 2686
Size: 248 704 1496 2809
Fragments arranged by size:
2809 1496 704 248
BsaWI w'CCGG_w
Cuts at: 181 235 2444 2965 3645 3919 4066 4477 181
Size: 54 2209 521 680 274 147 411 961
Fragments arranged by size:
2209 961 680 521 411 274 147 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1041 1071 1309 1339 1041
Size: 30 238 30 4959
Fragments arranged by size:
4959 238 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1947 1947
Size: 5257
BseYI C'CCAG_C
Cuts at: 1646 1947 2680 3438 4017 4513 1646
Size: 301 733 758 579 496 2390
Fragments arranged by size:
2390 758 733 579 496 301
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1374 1374
Size: 5257
BsiEI CG_ry'CG
Cuts at: 104 2797 2843 4053 104
Size: 2693 46 1210 1308
Fragments arranged by size:
2693 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 350 637 694 1445 1627 2269 3047 3237 4031
Size: 287 57 751 182 642 778 190 794
Cuts at: 4031 350
Size: 1576
Fragments arranged by size:
1576 794 778 751 642 287 190 182
57
BsmI GAATG_Cn'
Cuts at: 1977 1977
Size: 5257
BsmBI CGTCTCn'nnnn_
Cuts at: 426 426
Size: 5257
Bsp1286I G_dGCh'C
Cuts at: 350 637 694 790 1094 1445 1627 2269 2880
Size: 287 57 96 304 351 182 642 611
Cuts at: 2880 2973 3047 3237 3299 4031 350
Size: 93 74 190 62 732 1576
Fragments arranged by size:
1576 732 642 611 351 304 287 190
182 96 93 74 62 57
BspEI T'CCGG_A
Cuts at: 235 4477 235
Size: 4242 1015
BspHI T'CATG_A
Cuts at: 1512 4433 1512
Size: 2921 2336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1245 2305 2519 2821 3202 1245
Size: 1060 214 302 381 3300
Fragments arranged by size:
3300 1060 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2099 3547 3601 5087 5252 50
Size: 2049 1448 54 1486 165 55
Fragments arranged by size:
2049 1486 1448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3167 3167
Size: 5257
BsrFI r'CCGG_y
Cuts at: 1504 3253 3434 3645 1504
Size: 1749 181 211 3116
Fragments arranged by size:
3116 1749 211 181
BsrGI T'GTAC_A
Cuts at: 937 937
Size: 5257
BssSI C'ACGA_G
Cuts at: 1024 3526 3886 1024
Size: 2502 360 2395
Fragments arranged by size:
2502 2395 360
BstAPI GCAn_nnn'nTGC
Cuts at: 2632 2632
Size: 5257
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5257
BstEII G'GTnAC_C
Cuts at: 1047 1047
Size: 5257
BstXI CCAn_nnnn'nTGG
Cuts at: 1453 2086 2185 1453
Size: 633 99 4525
Fragments arranged by size:
4525 633 99
BstYI r'GATC_y
Cuts at: 1 145 719 1143 1182 1566 1652 2169 2280
Size: 144 574 424 39 384 86 517 111
Cuts at: 2280 2747 2770 3105 3351 4365 4451 4463 4809
Size: 467 23 335 246 1014 86 12 346
Cuts at: 4809 1
Size: 449
Fragments arranged by size:
1014 574 517 467 449 424 384 346
335 246 144 111 86 86 39 23
12
Bsu36I CC'TnA_GG
Cuts at: 1220 1220
Size: 5257
BtgI C'CryG_G
Cuts at: 125 170 216 1529 1757 2085 125
Size: 45 46 1313 228 328 3297
Fragments arranged by size:
3297 1313 328 228 46 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3205 3386 3549 3205
Size: 181 163 4913
Fragments arranged by size:
4913 181 163
BtsI GCAGTG_nn'
Cuts at: 2074 2498 2074
Size: 424 4833
ClaI AT'CG_AT
Cuts at: 1296 1296
Size: 5257
DraI TTT'AAA
Cuts at: 2254 2254
Size: 5257
DrdI GACnn_nn'nnGTC
Cuts at: 2961 3821 2961
Size: 860 4397
EaeI y'GGCC_r
Cuts at: 176 416 958 2088 2840 3014 3405 3432 4779
Size: 240 542 1130 752 174 391 27 1347
Cuts at: 4779 176
Size: 654
Fragments arranged by size:
1347 1130 752 654 542 391 240 174
27
EagI C'GGCC_G
Cuts at: 2840 2840
Size: 5257
EarI CTCTTCn'nnn_
Cuts at: 1713 1755 1929 3278 3488 1713
Size: 42 174 1349 210 3482
Fragments arranged by size:
3482 1349 210 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1555 1817 3463 3775 3921 5067 1555
Size: 262 1646 312 146 1146 1745
Fragments arranged by size:
1745 1646 1146 312 262 146
EcoICRI GAG'CTC
Cuts at: 348 1625 2267 348
Size: 1277 642 3338
Fragments arranged by size:
3338 1277 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 116 526 751 1004 1027 1399 1686 1694 1748
Size: 410 225 253 23 372 287 8 54
Cuts at: 1748 1926 1965 1985 2156 3080 3512 4261 4529
Size: 178 39 20 171 924 432 749 268
Cuts at: 4529 116
Size: 844
Fragments arranged by size:
924 844 749 432 410 372 287 268
253 225 178 171 54 39 23 20
8
EcoO109I rG'GnC_Cy
Cuts at: 268 1482 2394 268
Size: 1214 912 3131
Fragments arranged by size:
3131 1214 912
EcoRI G'AATT_C
Cuts at: 1207 2259 1207
Size: 1052 4205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 501 3274 501
Size: 2773 2484
FspI TGC'GCA
Cuts at: 3036 3036
Size: 5257
HaeII r_GCGC'y
Cuts at: 2689 2937 3961 4940 2689
Size: 248 1024 979 3006
Fragments arranged by size:
3006 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1041 1073 1090 1122 1444 1476 1729 1761 1806
Size: 32 17 32 322 32 253 32 45
Cuts at: 1806 1832 1838 1864 3097 3127 3129 3159 1041
Size: 26 6 26 1233 30 2 30 3139
Fragments arranged by size:
3139 1233 322 253 45 32 32 32
32 30 30 26 26 17 6 2
HincII GTy'rAC
Cuts at: 2294 2294
Size: 5257
KasI G'GCGC_C
Cuts at: 2685 2933 2685
Size: 248 5009
KpnI G_GTAC'C
Cuts at: 2275 2275
Size: 5257
MfeI C'AATT_G
Cuts at: 1031 1031
Size: 5257
MluI A'CGCG_T
Cuts at: 4690 4690
Size: 5257
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 2197 3928 4112 377
Size: 1820 1731 184 1522
Fragments arranged by size:
1820 1731 1522 184
MscI TGG'CCA
Cuts at: 960 2090 3016 960
Size: 1130 926 3201
Fragments arranged by size:
3201 1130 926
MslI CAynn'nnrTG
Cuts at: 659 775 1072 1138 1238 3244 3371 659
Size: 116 297 66 100 2006 127 2545
Fragments arranged by size:
2545 2006 297 127 116 100 66
MspA1I CmG'CkG
Cuts at: 529 2366 2680 3040 4055 4300 529
Size: 1837 314 360 1015 245 1486
Fragments arranged by size:
1837 1486 1015 360 314 245
NaeI GCC'GGC
Cuts at: 3436 3436
Size: 5257
NarI GG'CG_CC
Cuts at: 2686 2934 2686
Size: 248 5009
NcoI C'CATG_G
Cuts at: 125 170 2085 125
Size: 45 1915 3297
Fragments arranged by size:
3297 1915 45
NgoMIV G'CCGG_C
Cuts at: 3434 3434
Size: 5257
NruI TCG'CGA
Cuts at: 3618 3618
Size: 5257
NsiI A_TGCA'T
Cuts at: 1069 1069
Size: 5257
NspI r_CATG'y
Cuts at: 371 1773 2154 2226 2308 2808 3717 371
Size: 1402 381 72 82 500 909 1911
Fragments arranged by size:
1911 1402 909 500 381 82 72
PasI CC'CwG_GG
Cuts at: 2178 2178
Size: 5257
PciI A'CATG_T
Cuts at: 1769 3713 1769
Size: 1944 3313
PflMI CCAn_nnn'nTGG
Cuts at: 1257 1257
Size: 5257
PfoI T'CCnGG_A
Cuts at: 1690 1836 2144 1690
Size: 146 308 4803
Fragments arranged by size:
4803 308 146
PmeI GTTT'AAAC
Cuts at: 2254 2254
Size: 5257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4396 4428 4396
Size: 32 5225
PpuMI rG'GwC_Cy
Cuts at: 1482 1482
Size: 5257
PstI C_TGCA'G
Cuts at: 811 1638 2302 2987 811
Size: 827 664 685 3081
Fragments arranged by size:
3081 827 685 664
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5257
PvuII CAG'CTG
Cuts at: 529 2680 3040 529
Size: 2151 360 2746
Fragments arranged by size:
2746 2151 360
RsrII CG'GwC_CG
Cuts at: 3450 3450
Size: 5257
SacI G_AGCT'C
Cuts at: 350 1627 2269 350
Size: 1277 642 3338
Fragments arranged by size:
3338 1277 642
SalI G'TCGA_C
Cuts at: 2292 2292
Size: 5257
SapI GCTCTTCn'nnn_
Cuts at: 1713 3278 3488 1713
Size: 1565 210 3482
Fragments arranged by size:
3482 1565 210
SbfI CC_TGCA'GG
Cuts at: 2302 2302
Size: 5257
ScaI AGT'ACT
Cuts at: 4774 4774
Size: 5257
SfcI C'TryA_G
Cuts at: 33 807 1634 1929 2298 2983 3978 4169 33
Size: 774 827 295 369 685 995 191 1121
Fragments arranged by size:
1121 995 827 774 685 369 295 191
SfoI GGC'GCC
Cuts at: 2687 2935 2687
Size: 248 5009
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5257
SmaI CCC'GGG
Cuts at: 2277 2277
Size: 5257
SmlI C'TyrA_G
Cuts at: 2410 3325 3819 4081 2410
Size: 915 494 262 3586
Fragments arranged by size:
3586 915 494 262
SnaBI TAC'GTA
Cuts at: 2247 2247
Size: 5257
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5257
SphI G_CATG'C
Cuts at: 2308 2808 2308
Size: 500 4757
SspI AAT'ATT
Cuts at: 1018 1018
Size: 5257
StuI AGG'CCT
Cuts at: 955 1699 955
Size: 744 4513
StyI C'CwwG_G
Cuts at: 125 170 891 1662 2040 2085 2389 2743 125
Size: 45 721 771 378 45 304 354 2639
Fragments arranged by size:
2639 771 721 378 354 304 45 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1275 2838 2971 3430 3641 1275
Size: 1563 133 459 211 2891
Fragments arranged by size:
2891 1563 459 211 133
TatI w'GTAC_w
Cuts at: 335 937 1379 4772 335
Size: 602 442 3393 820
Fragments arranged by size:
3393 820 602 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 418 450 804 836 4774 4806 418
Size: 32 354 32 3938 32 869
Fragments arranged by size:
3938 869 354 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 358 589 1872 3052 358
Size: 231 1283 1180 2563
Fragments arranged by size:
2563 1283 1180 231
XbaI T'CTAG_A
Cuts at: 2286 2286
Size: 5257
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 411 2750 411
Size: 2339 2918
XmaI C'CCGG_G
Cuts at: 2275 2275
Size: 5257
XmnI GAAnn'nnTTC
Cuts at: 877 1699 877
Size: 822 4435
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BmtI Bpu10I BsiWI BssHII BstZ17I CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NdeI
NheI NotI PacI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SrfI SwaI
XhoI ZraI