Restriction map for
pF1KSDA0286
(Circular) (Six-base) MAPSORT of: pF1KSDA0286.seq Check: 8216 from: 1 to: 4469
pF1KSDA0286 4469 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1108 1108
Size: 4469
AccI GT'mk_AC
Cuts at: 242 1507 242
Size: 1265 3204
Acc65I G'GTAC_C
Cuts at: 1485 1485
Size: 4469
AclI AA'CG_TT
Cuts at: 4309 4309
Size: 4469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 457 2294 2726 3473 3741 265
Size: 192 1837 432 747 268 993
Fragments arranged by size:
1837 993 747 432 268 192
AflIII A'CryG_T
Cuts at: 1821 2925 3902 1821
Size: 1104 977 2388
Fragments arranged by size:
2388 1104 977
AgeI A'CCGG_T
Cuts at: 2857 2857
Size: 4469
AleI CACnn'nnGTG
Cuts at: 334 415 334
Size: 81 4388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 457 489 457
Size: 32 4437
AlwNI CAG_nnn'CTG
Cuts at: 1089 1774 3341 1089
Size: 685 1567 2217
Fragments arranged by size:
2217 1567 685
ApaI G_GGCC'C
Cuts at: 176 935 176
Size: 759 3710
ApaLI G'TGCA_C
Cuts at: 3239 3239
Size: 4469
ApoI r'AATT_y
Cuts at: 677 837 1216 1473 3822 4078 677
Size: 160 379 257 2349 256 1068
Fragments arranged by size:
2349 1068 379 257 256 160
AseI AT'TA_AT
Cuts at: 20 20
Size: 4469
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
AvaI C'yCGr_G
Cuts at: 169 183 1489 169
Size: 14 1306 3149
Fragments arranged by size:
3149 1306 14
BamHI G'GATC_C
Cuts at: 1494 1494
Size: 4469
BanI G'GyrC_C
Cuts at: 1485 1899 2147 2182 1485
Size: 414 248 35 3772
Fragments arranged by size:
3772 414 248 35
BanII G_rGCy'C
Cuts at: 176 184 935 1211 1483 2513 176
Size: 8 751 276 272 1030 2132
Fragments arranged by size:
2132 1030 751 276 272 8
BbeI G_GCGC'C
Cuts at: 1903 2151 1903
Size: 248 4221
BbsI GAAGACnn'nnnn_
Cuts at: 1255 1255
Size: 4469
BciVI GTATCCnnnnn_n'
Cuts at: 365 1163 2367 3128 4469 365
Size: 798 1204 761 1341 365
Fragments arranged by size:
1341 1204 798 761 365
BclI T'GATC_A
Cuts at: 1989 1989
Size: 4469
BglII A'GATC_T
Cuts at: 848 1984 4021 848
Size: 1136 2037 1296
Fragments arranged by size:
2037 1296 1136
BlpI GC'TnA_GC
Cuts at: 664 1581 664
Size: 917 3552
Bme1580I G_kGCm'C
Cuts at: 176 935 2094 2187 3243 176
Size: 759 1159 93 1056 1402
Fragments arranged by size:
1402 1159 1056 759 93
BmrI ACTGGGnnnn_n'
Cuts at: 704 1681 1762 2097 4241 704
Size: 977 81 335 2144 932
Fragments arranged by size:
2144 977 932 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1428 2836 1428
Size: 1408 3061
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 873 873
Size: 4469
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1645 2524 3016 3314 1645
Size: 879 492 298 2800
Fragments arranged by size:
2800 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 156 4173 156
Size: 4017 452
BsaAI yAC'GTr
Cuts at: 1461 1822 1461
Size: 361 4108
BsaBI GATnn'nnATC
Cuts at: 6 376 1983 4020 6
Size: 370 1607 2037 455
Fragments arranged by size:
2037 1607 455 370
BsaHI Gr'CG_yC
Cuts at: 1900 2148 2850 4346 1900
Size: 248 702 1496 2023
Fragments arranged by size:
2023 1496 702 248
BsaWI w'CCGG_w
Cuts at: 593 1658 2179 2857 3131 3278 3689 593
Size: 1065 521 678 274 147 411 1373
Fragments arranged by size:
1373 1065 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 442 1219 1417 1420 442
Size: 777 198 3 3491
Fragments arranged by size:
3491 777 198 3
BseYI C'CCAG_C
Cuts at: 1894 2652 3229 3725 1894
Size: 758 577 496 2638
Fragments arranged by size:
2638 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2011 2057 3265 104
Size: 1907 46 1208 1308
Fragments arranged by size:
1907 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1211 1483 2261 2451 3243 1211
Size: 272 778 190 792 2437
Fragments arranged by size:
2437 792 778 272 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1150 1150
Size: 4469
Bsp1286I G_dGCh'C
Cuts at: 176 184 935 1211 1483 2094 2187 2261 2451
Size: 8 751 276 272 611 93 74 190
Cuts at: 2451 2513 3243 176
Size: 62 730 1402
Fragments arranged by size:
1402 751 730 611 276 272 190 93
74 62 8
BspEI T'CCGG_A
Cuts at: 593 3689 593
Size: 3096 1373
BspHI T'CATG_A
Cuts at: 902 3645 902
Size: 2743 1726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 547 968 1108 1519 1733 2035 2416 547
Size: 421 140 411 214 302 381 2600
Fragments arranged by size:
2600 421 411 381 302 214 140
BsrBI CCG'CTC
Cuts at: 50 2761 2815 4299 4464 50
Size: 2711 54 1484 165 55
Fragments arranged by size:
2711 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 2381 1046
Size: 1335 3134
BsrFI r'CCGG_y
Cuts at: 156 2467 2648 2857 156
Size: 2311 181 209 1768
Fragments arranged by size:
2311 1768 209 181
BsrGI T'GTAC_A
Cuts at: 1091 1091
Size: 4469
BssSI C'ACGA_G
Cuts at: 2740 3098 2740
Size: 358 4111
BstAPI GCAn_nnn'nTGC
Cuts at: 1846 1846
Size: 4469
BstBI TT'CG_AA
Cuts at: 110 2830 110
Size: 2720 1749
BstYI r'GATC_y
Cuts at: 1 377 848 1494 1961 1984 2319 2565 3577
Size: 376 471 646 467 23 335 246 1012
Cuts at: 3577 3663 3675 4021 1
Size: 86 12 346 449
Fragments arranged by size:
1012 646 471 467 449 376 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 125 125
Size: 4469
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2419 2600 2763 2419
Size: 181 163 4125
Fragments arranged by size:
4125 181 163
BtsI GCAGTG_nn'
Cuts at: 1712 1712
Size: 4469
DraI TTT'AAA
Cuts at: 485 834 1468 485
Size: 349 634 3486
Fragments arranged by size:
3486 634 349
DrdI GACnn_nn'nnGTC
Cuts at: 2175 3033 2175
Size: 858 3611
EaeI y'GGCC_r
Cuts at: 2054 2228 2619 2646 3991 2054
Size: 174 391 27 1345 2532
Fragments arranged by size:
2532 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2054 2054
Size: 4469
EarI CTCTTCn'nnn_
Cuts at: 1308 2492 2702 1308
Size: 1184 210 3075
Fragments arranged by size:
3075 1184 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2677 2987 3133 4279 2677
Size: 310 146 1146 2867
Fragments arranged by size:
2867 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1209 1481 1209
Size: 272 4197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 457 873 2294 2726 3473 3741 265
Size: 192 416 1421 432 747 268 993
Fragments arranged by size:
1421 993 747 432 416 268 192
EcoO109I rG'GnC_Cy
Cuts at: 173 932 1608 173
Size: 759 676 3034
Fragments arranged by size:
3034 759 676
EcoRI G'AATT_C
Cuts at: 1473 1473
Size: 4469
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2488 2488
Size: 4469
FspI TGC'GCA
Cuts at: 2250 2250
Size: 4469
HaeII r_GCGC'y
Cuts at: 1903 2151 3173 4152 1903
Size: 248 1022 979 2220
Fragments arranged by size:
2220 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 588 620 657 689 1242 1274 1378 1410 2311
Size: 32 37 32 553 32 104 32 901
Cuts at: 2311 2341 2343 2373 588
Size: 30 2 30 2684
Fragments arranged by size:
2684 901 553 104 37 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 513 1508 513
Size: 995 3474
HpaI GTT'AAC
Cuts at: 513 513
Size: 4469
KasI G'GCGC_C
Cuts at: 1899 2147 1899
Size: 248 4221
KpnI G_GTAC'C
Cuts at: 1489 1489
Size: 4469
MluI A'CGCG_T
Cuts at: 3902 3902
Size: 4469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 876 3140 3324 876
Size: 2264 184 2021
Fragments arranged by size:
2264 2021 184
MscI TGG'CCA
Cuts at: 2230 2230
Size: 4469
MslI CAynn'nnrTG
Cuts at: 334 415 787 2458 2585 334
Size: 81 372 1671 127 2218
Fragments arranged by size:
2218 1671 372 127 81
MspA1I CmG'CkG
Cuts at: 253 981 1580 1894 2254 3267 3512 253
Size: 728 599 314 360 1013 245 1210
Fragments arranged by size:
1210 1013 728 599 360 314 245
NaeI GCC'GGC
Cuts at: 158 2650 158
Size: 2492 1977
NarI GG'CG_CC
Cuts at: 1900 2148 1900
Size: 248 4221
NcoI C'CATG_G
Cuts at: 125 125
Size: 4469
NgoMIV G'CCGG_C
Cuts at: 156 2648 156
Size: 2492 1977
NspI r_CATG'y
Cuts at: 1522 2022 2929 1522
Size: 500 907 3062
Fragments arranged by size:
3062 907 500
PasI CC'CwG_GG
Cuts at: 176 176
Size: 4469
PciI A'CATG_T
Cuts at: 2925 2925
Size: 4469
PmeI GTTT'AAAC
Cuts at: 1468 1468
Size: 4469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 3608
Size: 32 4437
PspOMI G'GGCC_C
Cuts at: 172 931 172
Size: 759 3710
PstI C_TGCA'G
Cuts at: 1105 1516 2201 1105
Size: 411 685 3373
Fragments arranged by size:
3373 685 411
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4469
PvuII CAG'CTG
Cuts at: 253 981 1894 2254 253
Size: 728 913 360 2468
Fragments arranged by size:
2468 913 728 360
RsrII CG'GwC_CG
Cuts at: 2664 2664
Size: 4469
SacI G_AGCT'C
Cuts at: 1211 1483 1211
Size: 272 4197
SalI G'TCGA_C
Cuts at: 1506 1506
Size: 4469
SapI GCTCTTCn'nnn_
Cuts at: 1308 2492 2702 1308
Size: 1184 210 3075
Fragments arranged by size:
3075 1184 210
SbfI CC_TGCA'GG
Cuts at: 1516 1516
Size: 4469
ScaI AGT'ACT
Cuts at: 3986 3986
Size: 4469
SfcI C'TryA_G
Cuts at: 33 1101 1512 2197 3190 3381 33
Size: 1068 411 685 993 191 1121
Fragments arranged by size:
1121 1068 993 685 411 191
SfoI GGC'GCC
Cuts at: 1901 2149 1901
Size: 248 4221
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4469
SmaI CCC'GGG
Cuts at: 171 1491 171
Size: 1320 3149
SmlI C'TyrA_G
Cuts at: 1624 2539 3031 3293 1624
Size: 915 492 262 2800
Fragments arranged by size:
2800 915 492 262
SnaBI TAC'GTA
Cuts at: 1461 1461
Size: 4469
SpeI A'CTAG_T
Cuts at: 67 825 67
Size: 758 3711
SphI G_CATG'C
Cuts at: 1522 2022 1522
Size: 500 3969
StyI C'CwwG_G
Cuts at: 125 935 1603 1957 125
Size: 810 668 354 2637
Fragments arranged by size:
2637 810 668 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 427 2052 2185 2644 2853 427
Size: 1625 133 459 209 2043
Fragments arranged by size:
2043 1625 459 209 133
TatI w'GTAC_w
Cuts at: 575 1057 1091 3984 575
Size: 482 34 2893 1060
Fragments arranged by size:
2893 1060 482 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 128 160 3986 4018 128
Size: 32 3826 32 579
Fragments arranged by size:
3826 579 32 32
Tth111I GACn'n_nGTC
Cuts at: 2266 2266
Size: 4469
XbaI T'CTAG_A
Cuts at: 1500 1500
Size: 4469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1964 1964
Size: 4469
XmaI C'CCGG_G
Cuts at: 169 1489 169
Size: 1320 3149
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI SspI StuI SwaI
XhoI XmnI ZraI