Restriction map for
pF1KSDA0282
(Circular) (Six-base) MAPSORT of: pF1KSDA0282.seq Check: 998 from: 1 to: 5131
pF1KSDA0282 5131 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 514 817 514
Size: 303 4828
AccI GT'mk_AC
Cuts at: 2167 2167
Size: 5131
Acc65I G'GTAC_C
Cuts at: 2145 2145
Size: 5131
AclI AA'CG_TT
Cuts at: 4971 4971
Size: 5131
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2954 3386 4135 4403 1183
Size: 1771 432 749 268 1911
Fragments arranged by size:
1911 1771 749 432 268
AfeI AGC'GCT
Cuts at: 1497 1497
Size: 5131
AflIII A'CryG_T
Cuts at: 2481 3587 4564 2481
Size: 1106 977 3048
Fragments arranged by size:
3048 1106 977
AgeI A'CCGG_T
Cuts at: 3519 3519
Size: 5131
AhdI GACnn_n'nnGTC
Cuts at: 1217 1217
Size: 5131
AlwNI CAG_nnn'CTG
Cuts at: 256 966 1128 2434 4003 256
Size: 710 162 1306 1569 1384
Fragments arranged by size:
1569 1384 1306 710 162
ApaI G_GGCC'C
Cuts at: 734 734
Size: 5131
ApaLI G'TGCA_C
Cuts at: 1303 1617 3901 1303
Size: 314 2284 2533
Fragments arranged by size:
2533 2284 314
ApoI r'AATT_y
Cuts at: 2133 4484 4740 2133
Size: 2351 256 2524
Fragments arranged by size:
2524 2351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5131
AvaI C'yCGr_G
Cuts at: 725 1658 2149 725
Size: 933 491 3707
Fragments arranged by size:
3707 933 491
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 483 516 483
Size: 33 5098
BamHI G'GATC_C
Cuts at: 2154 2154
Size: 5131
BanI G'GyrC_C
Cuts at: 483 645 753 1533 2145 2559 2807 2842 483
Size: 162 108 780 612 414 248 35 2772
Fragments arranged by size:
2772 780 612 414 248 162 108 35
BanII G_rGCy'C
Cuts at: 185 289 551 734 793 1874 2143 3173 185
Size: 104 262 183 59 1081 269 1030 2143
Fragments arranged by size:
2143 1081 1030 269 262 183 104 59
BbeI G_GCGC'C
Cuts at: 649 2563 2811 649
Size: 1914 248 2969
Fragments arranged by size:
2969 1914 248
BbsI GAAGACnn'nnnn_
Cuts at: 678 1624 1843 678
Size: 946 219 3966
Fragments arranged by size:
3966 946 219
BciVI GTATCCnnnnn_n'
Cuts at: 1540 3027 3790 5131 1540
Size: 1487 763 1341 1540
Fragments arranged by size:
1540 1487 1341 763
BclI T'GATC_A
Cuts at: 2649 2649
Size: 5131
BglI GCCn_nnn'nGGC
Cuts at: 729 729
Size: 5131
BglII A'GATC_T
Cuts at: 1181 2644 4683 1181
Size: 1463 2039 1629
Fragments arranged by size:
2039 1629 1463
BlpI GC'TnA_GC
Cuts at: 787 2241 787
Size: 1454 3677
Bme1580I G_kGCm'C
Cuts at: 488 734 758 1307 1538 1621 2754 2847 3905
Size: 246 24 549 231 83 1133 93 1058
Cuts at: 3905 488
Size: 1714
Fragments arranged by size:
1714 1133 1058 549 246 231 93 83
24
BmgBI CAC'GTC
Cuts at: 1932 1932
Size: 5131
BmrI ACTGGGnnnn_n'
Cuts at: 507 1875 2341 2422 2757 4903 507
Size: 1368 466 81 335 2146 735
Fragments arranged by size:
2146 1368 735 466 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 885 1424 1489 1576 847
Size: 38 539 65 87 4402
Fragments arranged by size:
4402 539 87 65 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1389 2305 3184 3678 3976 607
Size: 782 916 879 494 298 1762
Fragments arranged by size:
1762 916 879 782 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 300 807 1225 4835 300
Size: 507 418 3610 596
Fragments arranged by size:
3610 596 507 418
BsaAI yAC'GTr
Cuts at: 2121 2482 2121
Size: 361 4770
BsaBI GATnn'nnATC
Cuts at: 6 2643 4682 6
Size: 2637 2039 455
Fragments arranged by size:
2637 2039 455
BsaHI Gr'CG_yC
Cuts at: 419 646 1286 2560 2808 3512 5008 419
Size: 227 640 1274 248 704 1496 542
Fragments arranged by size:
1496 1274 704 640 542 248 227
BsaWI w'CCGG_w
Cuts at: 1994 2318 2839 3519 3793 3940 4351 1994
Size: 324 521 680 274 147 411 2774
Fragments arranged by size:
2774 680 521 411 324 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1326 1356 1600 1630 1326
Size: 30 244 30 4827
Fragments arranged by size:
4827 244 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 157 901 2065 145
Size: 12 744 1164 3211
Fragments arranged by size:
3211 1164 744 12
BseYI C'CCAG_C
Cuts at: 1121 1446 2554 3312 3891 4387 1121
Size: 325 1108 758 579 496 1865
Fragments arranged by size:
1865 1108 758 579 496 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 795 1176 262
Size: 533 381 4217
Fragments arranged by size:
4217 533 381
BsiEI CG_ry'CG
Cuts at: 104 2671 2717 3927 104
Size: 2567 46 1210 1308
Fragments arranged by size:
2567 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1157 1307 1621 1874 2143 2921 3111 3905 1157
Size: 150 314 253 269 778 190 794 2383
Fragments arranged by size:
2383 794 778 314 269 253 190 150
Bsp1286I G_dGCh'C
Cuts at: 185 289 488 551 734 758 793 1157 1307
Size: 104 199 63 183 24 35 364 150
Cuts at: 1307 1538 1621 1874 2143 2754 2847 2921 3111
Size: 231 83 253 269 611 93 74 190
Cuts at: 3111 3173 3905 185
Size: 62 732 1411
Fragments arranged by size:
1411 732 611 364 269 253 231 199
190 183 150 104 93 83 74 63
62 35 24
BspEI T'CCGG_A
Cuts at: 4351 4351
Size: 5131
BspHI T'CATG_A
Cuts at: 4307 4307
Size: 5131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 514 817 2179 2393 2695 3076 514
Size: 303 1362 214 302 381 2569
Fragments arranged by size:
2569 1362 381 303 302 214
BsrBI CCG'CTC
Cuts at: 50 3421 3475 4961 5126 50
Size: 3371 54 1486 165 55
Fragments arranged by size:
3371 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 859 1982 3041 859
Size: 1123 1059 2949
Fragments arranged by size:
2949 1123 1059
BsrFI r'CCGG_y
Cuts at: 450 468 3127 3308 3519 450
Size: 18 2659 181 211 2062
Fragments arranged by size:
2659 2062 211 181 18
BssSI C'ACGA_G
Cuts at: 820 3400 3760 820
Size: 2580 360 2191
Fragments arranged by size:
2580 2191 360
BstAPI GCAn_nnn'nTGC
Cuts at: 845 2506 845
Size: 1661 3470
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5131
BstYI r'GATC_y
Cuts at: 1 1037 1181 2154 2621 2644 2979 3225 4239
Size: 1036 144 973 467 23 335 246 1014
Cuts at: 4239 4325 4337 4683 1
Size: 86 12 346 449
Fragments arranged by size:
1036 1014 973 467 449 346 335 246
144 86 23 12
BtgI C'CryG_G
Cuts at: 125 281 552 614 1355 1529 125
Size: 156 271 62 741 174 3727
Fragments arranged by size:
3727 741 271 174 156 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 695 3079 3260 3423 695
Size: 2384 181 163 2403
Fragments arranged by size:
2403 2384 181 163
BtsI GCAGTG_nn'
Cuts at: 1188 2372 1188
Size: 1184 3947
ClaI AT'CG_AT
Cuts at: 1097 1097
Size: 5131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1344 1379 1964 1999 1344
Size: 35 585 35 4476
Fragments arranged by size:
4476 585 35 35
DraI TTT'AAA
Cuts at: 2128 2128
Size: 5131
DrdI GACnn_nn'nnGTC
Cuts at: 252 2835 3695 252
Size: 2583 860 1688
Fragments arranged by size:
2583 1688 860
EaeI y'GGCC_r
Cuts at: 452 2714 2888 3279 3306 4653 452
Size: 2262 174 391 27 1347 930
Fragments arranged by size:
2262 1347 930 391 174 27
EagI C'GGCC_G
Cuts at: 2714 2714
Size: 5131
EarI CTCTTCn'nnn_
Cuts at: 134 1293 3152 3362 134
Size: 1159 1859 210 1903
Fragments arranged by size:
1903 1859 1159 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 361 3337 3649 3795 4941 361
Size: 2976 312 146 1146 551
Fragments arranged by size:
2976 1146 551 312 146
EcoICRI GAG'CTC
Cuts at: 1872 2141 1872
Size: 269 4862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 885 1183 1424 1489 1576 2954 3386 4135
Size: 38 298 241 65 87 1378 432 749
Cuts at: 4135 4403 847
Size: 268 1575
Fragments arranged by size:
1575 1378 749 432 298 268 241 87
65 38
EcoNI CCTnn'n_nnAGG
Cuts at: 1312 1312
Size: 5131
EcoO109I rG'GnC_Cy
Cuts at: 730 731 2070 2268 730
Size: 1 1339 198 3593
Fragments arranged by size:
3593 1339 198 1
EcoRI G'AATT_C
Cuts at: 2133 2133
Size: 5131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1860 3148 1860
Size: 1288 3843
FspI TGC'GCA
Cuts at: 1021 2910 1021
Size: 1889 3242
HaeII r_GCGC'y
Cuts at: 386 649 967 1499 2563 2811 3835 4814 386
Size: 263 318 532 1064 248 1024 979 703
Fragments arranged by size:
1064 1024 979 703 532 318 263 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 244 276 1166 1168 1198 1200 1203 1235 1302
Size: 32 890 2 30 2 3 32 67
Cuts at: 1302 1334 1738 1770 2971 3001 3003 3033 244
Size: 32 404 32 1201 30 2 30 2342
Fragments arranged by size:
2342 1201 890 404 67 32 32 32
32 30 30 30 3 2 2 2
HincII GTy'rAC
Cuts at: 1140 2168 1140
Size: 1028 4103
HindIII A'AGCT_T
Cuts at: 1430 1967 1430
Size: 537 4594
KasI G'GCGC_C
Cuts at: 645 2559 2807 645
Size: 1914 248 2969
Fragments arranged by size:
2969 1914 248
KpnI G_GTAC'C
Cuts at: 2149 2149
Size: 5131
MfeI C'AATT_G
Cuts at: 2063 2063
Size: 5131
MluI A'CGCG_T
Cuts at: 4564 4564
Size: 5131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 323 3802 3986 323
Size: 3479 184 1468
Fragments arranged by size:
3479 1468 184
MscI TGG'CCA
Cuts at: 2890 2890
Size: 5131
MslI CAynn'nnrTG
Cuts at: 238 664 1930 3118 3245 238
Size: 426 1266 1188 127 2124
Fragments arranged by size:
2124 1266 1188 426 127
MspA1I CmG'CkG
Cuts at: 283 353 554 616 1017 1125 1410 1467 2240
Size: 70 201 62 401 108 285 57 773
Cuts at: 2240 2554 2914 3929 4174 283
Size: 314 360 1015 245 1240
Fragments arranged by size:
1240 1015 773 401 360 314 285 245
201 108 70 62 57
NaeI GCC'GGC
Cuts at: 3310 3310
Size: 5131
NarI GG'CG_CC
Cuts at: 646 2560 2808 646
Size: 1914 248 2969
Fragments arranged by size:
2969 1914 248
NcoI C'CATG_G
Cuts at: 125 125
Size: 5131
NgoMIV G'CCGG_C
Cuts at: 3308 3308
Size: 5131
NruI TCG'CGA
Cuts at: 3492 3492
Size: 5131
NspI r_CATG'y
Cuts at: 843 2182 2682 3591 843
Size: 1339 500 909 2383
Fragments arranged by size:
2383 1339 909 500
PasI CC'CwG_GG
Cuts at: 765 765
Size: 5131
PciI A'CATG_T
Cuts at: 3587 3587
Size: 5131
PflMI CCAn_nnn'nTGG
Cuts at: 825 882 1128 1410 825
Size: 57 246 282 4546
Fragments arranged by size:
4546 282 246 57
PfoI T'CCnGG_A
Cuts at: 533 1040 1507 1586 533
Size: 507 467 79 4078
Fragments arranged by size:
4078 507 467 79
PmeI GTTT'AAAC
Cuts at: 2128 2128
Size: 5131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4270 4302 4270
Size: 32 5099
PpuMI rG'GwC_Cy
Cuts at: 2070 2070
Size: 5131
PshAI GACnn'nnGTC
Cuts at: 1370 1708 1370
Size: 338 4793
PspOMI G'GGCC_C
Cuts at: 730 730
Size: 5131
PstI C_TGCA'G
Cuts at: 2176 2861 2176
Size: 685 4446
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5131
PvuII CAG'CTG
Cuts at: 1017 1125 1410 1467 2554 2914 1017
Size: 108 285 57 1087 360 3234
Fragments arranged by size:
3234 1087 360 285 108 57
RsrII CG'GwC_CG
Cuts at: 3324 3324
Size: 5131
SacI G_AGCT'C
Cuts at: 1874 2143 1874
Size: 269 4862
SacII CC_GC'GG
Cuts at: 284 555 617 284
Size: 271 62 4798
Fragments arranged by size:
4798 271 62
SalI G'TCGA_C
Cuts at: 2166 2166
Size: 5131
SapI GCTCTTCn'nnn_
Cuts at: 3152 3362 3152
Size: 210 4921
SbfI CC_TGCA'GG
Cuts at: 2176 2176
Size: 5131
ScaI AGT'ACT
Cuts at: 1231 4648 1231
Size: 3417 1714
SfcI C'TryA_G
Cuts at: 33 1462 1803 2172 2857 3852 4043 33
Size: 1429 341 369 685 995 191 1121
Fragments arranged by size:
1429 1121 995 685 369 341 191
SfoI GGC'GCC
Cuts at: 647 2561 2809 647
Size: 1914 248 2969
Fragments arranged by size:
2969 1914 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5131
SmaI CCC'GGG
Cuts at: 727 2151 727
Size: 1424 3707
SmlI C'TyrA_G
Cuts at: 622 1368 2284 3199 3693 3955 622
Size: 746 916 915 494 262 1798
Fragments arranged by size:
1798 916 915 746 494 262
SnaBI TAC'GTA
Cuts at: 2121 2121
Size: 5131
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5131
SphI G_CATG'C
Cuts at: 2182 2682 2182
Size: 500 4631
StuI AGG'CCT
Cuts at: 1669 1669
Size: 5131
StyI C'CwwG_G
Cuts at: 125 650 998 1345 1850 2263 2617 125
Size: 525 348 347 505 413 354 2639
Fragments arranged by size:
2639 525 505 413 354 348 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 472 2712 2845 3304 3515 472
Size: 2240 133 459 211 2088
Fragments arranged by size:
2240 2088 459 211 133
TatI w'GTAC_w
Cuts at: 842 1229 4646 842
Size: 387 3417 1327
Fragments arranged by size:
3417 1327 387
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1326 1358 1600 1632 4648 4680 1326
Size: 32 242 32 3016 32 1777
Fragments arranged by size:
3016 1777 242 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2926 2926
Size: 5131
XbaI T'CTAG_A
Cuts at: 2160 2160
Size: 5131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 132 747 1005 1221 1407 2624 132
Size: 615 258 216 186 1217 2639
Fragments arranged by size:
2639 1217 615 258 216 186
XmaI C'CCGG_G
Cuts at: 725 2149 725
Size: 1424 3707
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AleI AlfI AloI AscI AvrII BbvCI
BcgI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI HpaI NdeI NheI NotI NsiI PacI PmlI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SrfI
SspI SwaI XhoI XmnI ZraI