Restriction map for
pF1KSDA0280
(Circular) (Six-base) MAPSORT of: pF1KSDA0280.seq Check: 6269 from: 1 to: 3871
pF1KSDA0280 3871 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 907 907
Size: 3871
Acc65I G'GTAC_C
Cuts at: 748 885 748
Size: 137 3734
AclI AA'CG_TT
Cuts at: 3711 3711
Size: 3871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1694 2126 2875 3143 377
Size: 1317 432 749 268 1105
Fragments arranged by size:
1317 1105 749 432 268
AflIII A'CryG_T
Cuts at: 1221 2327 3304 1221
Size: 1106 977 1788
Fragments arranged by size:
1788 1106 977
AgeI A'CCGG_T
Cuts at: 2259 2259
Size: 3871
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 328 360 328
Size: 32 3839
AlwNI CAG_nnn'CTG
Cuts at: 220 288 561 676 1174 2743 220
Size: 68 273 115 498 1569 1348
Fragments arranged by size:
1569 1348 498 273 115 68
ApaI G_GGCC'C
Cuts at: 819 819
Size: 3871
ApaLI G'TGCA_C
Cuts at: 2641 2641
Size: 3871
ApoI r'AATT_y
Cuts at: 873 3224 3480 873
Size: 2351 256 1264
Fragments arranged by size:
2351 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3871
AvaI C'yCGr_G
Cuts at: 687 889 687
Size: 202 3669
BamHI G'GATC_C
Cuts at: 894 894
Size: 3871
BanI G'GyrC_C
Cuts at: 361 724 748 885 1299 1547 1582 361
Size: 363 24 137 414 248 35 2650
Fragments arranged by size:
2650 414 363 248 137 35 24
BanII G_rGCy'C
Cuts at: 162 819 883 1913 162
Size: 657 64 1030 2120
Fragments arranged by size:
2120 1030 657 64
BbeI G_GCGC'C
Cuts at: 1303 1551 1303
Size: 248 3623
BbsI GAAGACnn'nnnn_
Cuts at: 335 335
Size: 3871
BciVI GTATCCnnnnn_n'
Cuts at: 539 1767 2530 3871 539
Size: 1228 763 1341 539
Fragments arranged by size:
1341 1228 763 539
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3871
BglII A'GATC_T
Cuts at: 1384 3423 1384
Size: 2039 1832
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3871
Bme1580I G_kGCm'C
Cuts at: 789 819 848 1494 1587 2645 789
Size: 30 29 646 93 1058 2015
Fragments arranged by size:
2015 1058 646 93 30 29
BmrI ACTGGGnnnn_n'
Cuts at: 257 370 1081 1162 1497 3643 257
Size: 113 711 81 335 2146 485
Fragments arranged by size:
2146 711 485 335 113 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 257
Size: 3871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2418 2716 1045
Size: 879 494 298 2200
Fragments arranged by size:
2200 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 3575 3575
Size: 3871
BsaAI yAC'GTr
Cuts at: 843 861 1222 843
Size: 18 361 3492
Fragments arranged by size:
3492 361 18
BsaBI GATnn'nnATC
Cuts at: 6 1383 3422 6
Size: 1377 2039 455
Fragments arranged by size:
2039 1377 455
BsaHI Gr'CG_yC
Cuts at: 1300 1548 2252 3748 1300
Size: 248 704 1496 1423
Fragments arranged by size:
1496 1423 704 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2259 2533 2680 3091 1058
Size: 521 680 274 147 411 1838
Fragments arranged by size:
1838 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 781 811 781
Size: 30 3841
BseYI C'CCAG_C
Cuts at: 238 551 605 669 767 1294 2052 2631 3127
Size: 313 54 64 98 527 758 579 496
Cuts at: 3127 238
Size: 982
Fragments arranged by size:
982 758 579 527 496 313 98 64
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 3871
BsiEI CG_ry'CG
Cuts at: 104 635 1411 1457 2667 104
Size: 531 776 46 1210 1308
Fragments arranged by size:
1308 1210 776 531 46
BsiHKAI G_wGCw'C
Cuts at: 883 1661 1851 2645 883
Size: 778 190 794 2109
Fragments arranged by size:
2109 794 778 190
BsmI GAATG_Cn'
Cuts at: 291 650 291
Size: 359 3512
Bsp1286I G_dGCh'C
Cuts at: 162 789 819 848 883 1494 1587 1661 1851
Size: 627 30 29 35 611 93 74 190
Cuts at: 1851 1913 2645 162
Size: 62 732 1388
Fragments arranged by size:
1388 732 627 611 190 93 74 62
35 30 29
BspEI T'CCGG_A
Cuts at: 3091 3091
Size: 3871
BspHI T'CATG_A
Cuts at: 3047 3047
Size: 3871
BspMI ACCTGCnnnn'nnnn_
Cuts at: 736 919 1133 1435 1816 736
Size: 183 214 302 381 2791
Fragments arranged by size:
2791 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3701 3866 50
Size: 2111 54 1486 165 55
Fragments arranged by size:
2111 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 743 1781 743
Size: 1038 2833
BsrFI r'CCGG_y
Cuts at: 591 1867 2048 2259 591
Size: 1276 181 211 2203
Fragments arranged by size:
2203 1276 211 181
BssSI C'ACGA_G
Cuts at: 2140 2500 2140
Size: 360 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 418 679 1246 418
Size: 261 567 3043
Fragments arranged by size:
3043 567 261
BstBI TT'CG_AA
Cuts at: 110 110
Size: 3871
BstEII G'GTnAC_C
Cuts at: 196 196
Size: 3871
BstXI CCAn_nnnn'nTGG
Cuts at: 702 714 702
Size: 12 3859
BstYI r'GATC_y
Cuts at: 1 894 1361 1384 1719 1965 2979 3065 3077
Size: 893 467 23 335 246 1014 86 12
Cuts at: 3077 3423 1
Size: 346 449
Fragments arranged by size:
1014 893 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 629 707 731 629
Size: 78 24 3769
Fragments arranged by size:
3769 78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1819 2000 2163 1819
Size: 181 163 3527
Fragments arranged by size:
3527 181 163
BtsI GCAGTG_nn'
Cuts at: 476 1112 476
Size: 636 3235
DraI TTT'AAA
Cuts at: 868 868
Size: 3871
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2435 1575
Size: 860 3011
EaeI y'GGCC_r
Cuts at: 328 521 704 1454 1628 2019 2046 3393 328
Size: 193 183 750 174 391 27 1347 806
Fragments arranged by size:
1347 806 750 391 193 183 174 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3871
EarI CTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3661
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2077 2389 2535 3681 2077
Size: 312 146 1146 2267
Fragments arranged by size:
2267 1146 312 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 377 1694 2126 2875 3143 257
Size: 120 1317 432 749 268 985
Fragments arranged by size:
1317 985 749 432 268 120
EcoO109I rG'GnC_Cy
Cuts at: 456 815 1008 456
Size: 359 193 3319
Fragments arranged by size:
3319 359 193
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3871
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3871
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3871
HaeII r_GCGC'y
Cuts at: 1303 1551 2575 3554 1303
Size: 248 1024 979 1620
Fragments arranged by size:
1620 1024 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1711 1741 1743 1773 1711
Size: 30 2 30 3809
Fragments arranged by size:
3809 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3871
KasI G'GCGC_C
Cuts at: 1299 1547 1299
Size: 248 3623
KpnI G_GTAC'C
Cuts at: 752 889 752
Size: 137 3734
MluI A'CGCG_T
Cuts at: 3304 3304
Size: 3871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2542 2726 2542
Size: 184 3687
MscI TGG'CCA
Cuts at: 330 706 1630 330
Size: 376 924 2571
Fragments arranged by size:
2571 924 376
MslI CAynn'nnrTG
Cuts at: 712 730 736 1858 1985 712
Size: 18 6 1122 127 2598
Fragments arranged by size:
2598 1122 127 18 6
MspA1I CmG'CkG
Cuts at: 220 980 1294 1654 2669 2914 220
Size: 760 314 360 1015 245 1177
Fragments arranged by size:
1177 1015 760 360 314 245
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3871
NarI GG'CG_CC
Cuts at: 1300 1548 1300
Size: 248 3623
NcoI C'CATG_G
Cuts at: 707 731 707
Size: 24 3847
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3871
NruI TCG'CGA
Cuts at: 2232 2232
Size: 3871
NspI r_CATG'y
Cuts at: 922 1422 2331 922
Size: 500 909 2462
Fragments arranged by size:
2462 909 500
PasI CC'CwG_GG
Cuts at: 570 570
Size: 3871
PciI A'CATG_T
Cuts at: 2327 2327
Size: 3871
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 268 300 3010 3042 268
Size: 32 2710 32 1097
Fragments arranged by size:
2710 1097 32 32
PpuMI rG'GwC_Cy
Cuts at: 456 456
Size: 3871
PspOMI G'GGCC_C
Cuts at: 815 815
Size: 3871
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 121 153 172 204 388 420 121
Size: 32 19 32 184 32 3572
Fragments arranged by size:
3572 184 32 32 32 19
PstI C_TGCA'G
Cuts at: 916 1601 916
Size: 685 3186
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3871
PvuII CAG'CTG
Cuts at: 220 1294 1654 220
Size: 1074 360 2437
Fragments arranged by size:
2437 1074 360
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3871
SacI G_AGCT'C
Cuts at: 883 883
Size: 3871
SalI G'TCGA_C
Cuts at: 906 906
Size: 3871
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3661
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3871
ScaI AGT'ACT
Cuts at: 3388 3388
Size: 3871
SfcI C'TryA_G
Cuts at: 33 912 1597 2592 2783 33
Size: 879 685 995 191 1121
Fragments arranged by size:
1121 995 879 685 191
SfoI GGC'GCC
Cuts at: 1301 1549 1301
Size: 248 3623
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3871
SmaI CCC'GGG
Cuts at: 891 891
Size: 3871
SmlI C'TyrA_G
Cuts at: 1024 1939 2433 2695 1024
Size: 915 494 262 2200
Fragments arranged by size:
2200 915 494 262
SnaBI TAC'GTA
Cuts at: 861 861
Size: 3871
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3871
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3371
StuI AGG'CCT
Cuts at: 325 325
Size: 3871
StyI C'CwwG_G
Cuts at: 707 731 819 1003 1357 707
Size: 24 88 184 354 3221
Fragments arranged by size:
3221 354 184 88 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 519 1452 1585 2044 2255 406
Size: 113 933 133 459 211 2022
Fragments arranged by size:
2022 933 459 211 133 113
TatI w'GTAC_w
Cuts at: 3386 3386
Size: 3871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 195 227 243 275 684 716 781 813 3388
Size: 32 16 32 409 32 65 32 2575
Cuts at: 3388 3420 195
Size: 32 646
Fragments arranged by size:
2575 646 409 65 32 32 32 32
32 16
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3871
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 714 1364 714
Size: 650 3221
XmaI C'CCGG_G
Cuts at: 889 889
Size: 3871
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PciI PmeI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI