Restriction map for
pF1KSDA0278
(Circular) (Six-base) MAPSORT of: pF1KSDA0278.seq Check: 140 from: 1 to: 4325
pF1KSDA0278 4325 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 177 278 282 804 177
Size: 101 4 522 3698
Fragments arranged by size:
3698 522 101 4
AccI GT'mk_AC
Cuts at: 1363 1363
Size: 4325
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 2150 2582 3329 3597 328
Size: 1822 432 747 268 1056
Fragments arranged by size:
1822 1056 747 432 268
AfeI AGC'GCT
Cuts at: 382 445 499 382
Size: 63 54 4208
Fragments arranged by size:
4208 63 54
AflIII A'CryG_T
Cuts at: 467 1677 2781 3758 467
Size: 1210 1104 977 1034
Fragments arranged by size:
1210 1104 1034 977
AgeI A'CCGG_T
Cuts at: 320 2713 320
Size: 2393 1932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1204 1204
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 664 1029 1630 3197 664
Size: 365 601 1567 1792
Fragments arranged by size:
1792 1567 601 365
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 1329 3678 3934 1329
Size: 2349 256 1720
Fragments arranged by size:
2349 1720 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 169 589 676 776 965 1309 1345 169
Size: 420 87 100 189 344 36 3149
Fragments arranged by size:
3149 420 344 189 100 87 36
BamHI G'GATC_C
Cuts at: 1350 1350
Size: 4325
BanI G'GyrC_C
Cuts at: 266 1105 1341 1755 2003 2038 266
Size: 839 236 414 248 35 2553
Fragments arranged by size:
2553 839 414 248 236 35
BanII G_rGCy'C
Cuts at: 158 689 904 1339 2369 158
Size: 531 215 435 1030 2114
Fragments arranged by size:
2114 1030 531 435 215
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4077
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2984 4325 2223
Size: 761 1341 2223
Fragments arranged by size:
2223 1341 761
BclI T'GATC_A
Cuts at: 1845 1845
Size: 4325
BglI GCCn_nnn'nGGC
Cuts at: 1219 1219
Size: 4325
BglII A'GATC_T
Cuts at: 761 1840 3877 761
Size: 1079 2037 1209
Fragments arranged by size:
2037 1209 1079
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4325
Bme1580I G_kGCm'C
Cuts at: 365 958 1108 1950 2043 3099 365
Size: 593 150 842 93 1056 1591
Fragments arranged by size:
1591 1056 842 593 150 93
BmrI ACTGGGnnnn_n'
Cuts at: 924 1537 1618 1953 4097 924
Size: 613 81 335 2144 1152
Fragments arranged by size:
2144 1152 613 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 358 460 514 535 1180 1222 262
Size: 96 102 54 21 645 42 3365
Fragments arranged by size:
3365 645 102 96 54 42 21
Bpu10I CC'TnA_GC
Cuts at: 787 787
Size: 4325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1108 1501 2380 2872 3170 1108
Size: 393 879 492 298 2263
Fragments arranged by size:
2263 879 492 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 1249 4029 418
Size: 831 2780 714
Fragments arranged by size:
2780 831 714
BsaAI yAC'GTr
Cuts at: 249 360 468 1065 1098 1317 1678 249
Size: 111 108 597 33 219 361 2896
Fragments arranged by size:
2896 597 361 219 111 108 33
BsaBI GATnn'nnATC
Cuts at: 6 1839 3876 6
Size: 1833 2037 455
Fragments arranged by size:
2037 1833 455
BsaHI Gr'CG_yC
Cuts at: 1756 2004 2706 4202 1756
Size: 248 702 1496 1879
Fragments arranged by size:
1879 1496 702 248
BsaWI w'CCGG_w
Cuts at: 140 320 1298 1514 2035 2713 2987 3134 3545
Size: 180 978 216 521 678 274 147 411
Cuts at: 3545 140
Size: 920
Fragments arranged by size:
978 920 678 521 411 274 216 180
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 814 844 1093 1096 814
Size: 30 249 3 4043
Fragments arranged by size:
4043 249 30 3
BseYI C'CCAG_C
Cuts at: 446 500 657 698 739 1304 1750 2508 3085
Size: 54 157 41 41 565 446 758 577
Cuts at: 3085 3581 446
Size: 496 1190
Fragments arranged by size:
1190 758 577 565 496 446 157 54
41 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1210 301
Size: 909 3416
BsiEI CG_ry'CG
Cuts at: 104 325 329 510 1214 1223 1867 1913 3121
Size: 221 4 181 704 9 644 46 1208
Cuts at: 3121 104
Size: 1308
Fragments arranged by size:
1308 1208 704 644 221 181 46 9
4
BsiHKAI G_wGCw'C
Cuts at: 248 1339 2117 2307 3099 248
Size: 1091 778 190 792 1474
Fragments arranged by size:
1474 1091 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 158 248 365 689 904 958 1108 1339 1950
Size: 90 117 324 215 54 150 231 611
Cuts at: 1950 2043 2117 2307 2369 3099 158
Size: 93 74 190 62 730 1384
Fragments arranged by size:
1384 730 611 324 231 215 190 150
117 93 90 74 62 54
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 177 278 282 804 1001 1375 1589 1891 2272
Size: 101 4 522 197 374 214 302 381
Cuts at: 2272 177
Size: 2230
Fragments arranged by size:
2230 522 381 374 302 214 197 101
4
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4155 4320 50
Size: 2567 54 1484 165 55
Fragments arranged by size:
2567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2237 2237
Size: 4325
BsrFI r'CCGG_y
Cuts at: 263 320 870 1218 1222 2323 2504 2713 263
Size: 57 550 348 4 1101 181 209 1875
Fragments arranged by size:
1875 1101 550 348 209 181 57 4
BssSI C'ACGA_G
Cuts at: 366 604 2596 2954 366
Size: 238 1992 358 1737
Fragments arranged by size:
1992 1737 358 238
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 110 2686 110
Size: 2576 1749
BstXI CCAn_nnnn'nTGG
Cuts at: 132 294 1158 132
Size: 162 864 3299
Fragments arranged by size:
3299 864 162
BstYI r'GATC_y
Cuts at: 1 761 848 1350 1817 1840 2175 2421 3433
Size: 760 87 502 467 23 335 246 1012
Cuts at: 3433 3519 3531 3877 1
Size: 86 12 346 449
Fragments arranged by size:
1012 760 502 467 449 346 335 246
87 86 23 12
BtgI C'CryG_G
Cuts at: 125 175 521 572 712 1286 125
Size: 50 346 51 140 574 3164
Fragments arranged by size:
3164 574 346 140 51 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 414 2275 2456 2619 414
Size: 1861 181 163 2120
Fragments arranged by size:
2120 1861 181 163
BtsI GCAGTG_nn'
Cuts at: 1568 1568
Size: 4325
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 628 2031 2889 628
Size: 1403 858 2064
Fragments arranged by size:
2064 1403 858
EaeI y'GGCC_r
Cuts at: 191 275 872 1211 1220 1289 1910 2084 2475
Size: 84 597 339 9 69 621 174 391
Cuts at: 2475 2502 3847 191
Size: 27 1345 669
Fragments arranged by size:
1345 669 621 597 391 339 174 84
69 27 9
EagI C'GGCC_G
Cuts at: 1211 1220 1910 1211
Size: 9 690 3626
Fragments arranged by size:
3626 690 9
EarI CTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 270 1267 2533 2843 2989 4135 270
Size: 997 1266 310 146 1146 460
Fragments arranged by size:
1266 1146 997 460 310 146
EcoICRI GAG'CTC
Cuts at: 1337 1337
Size: 4325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 328 358 460 514 535 1180 1222 2150
Size: 66 30 102 54 21 645 42 928
Cuts at: 2150 2582 3329 3597 262
Size: 432 747 268 990
Fragments arranged by size:
990 928 747 645 432 268 102 66
54 42 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 1204 1204
Size: 4325
EcoO109I rG'GnC_Cy
Cuts at: 586 717 771 1044 1464 586
Size: 131 54 273 420 3447
Fragments arranged by size:
3447 420 273 131 54
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4325
FseI GG_CCGG'CC
Cuts at: 874 1226 874
Size: 352 3973
FspI TGC'GCA
Cuts at: 2106 2106
Size: 4325
HaeII r_GCGC'y
Cuts at: 384 447 501 1759 2007 3029 4008 384
Size: 63 54 1258 248 1022 979 701
Fragments arranged by size:
1258 1022 979 701 248 63 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 840 872 959 991 2167 2197 2199 2229 840
Size: 32 87 32 1176 30 2 30 2936
Fragments arranged by size:
2936 1176 87 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4325
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4077
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2996 3180 2996
Size: 184 4141
MscI TGG'CCA
Cuts at: 193 1291 2086 193
Size: 1098 795 2432
Fragments arranged by size:
2432 1098 795
MslI CAynn'nnrTG
Cuts at: 1186 2314 2441 1186
Size: 1128 127 3070
Fragments arranged by size:
3070 1128 127
MspA1I CmG'CkG
Cuts at: 149 177 416 664 981 1017 1436 1750 2110
Size: 28 239 248 317 36 419 314 360
Cuts at: 2110 3123 3368 149
Size: 1013 245 1106
Fragments arranged by size:
1106 1013 419 360 317 314 248 245
239 36 28
NaeI GCC'GGC
Cuts at: 872 1220 1224 2506 872
Size: 348 4 1282 2691
Fragments arranged by size:
2691 1282 348 4
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4077
NcoI C'CATG_G
Cuts at: 125 125
Size: 4325
NgoMIV G'CCGG_C
Cuts at: 870 1218 1222 2504 870
Size: 348 4 1282 2691
Fragments arranged by size:
2691 1282 348 4
NspI r_CATG'y
Cuts at: 1378 1878 2785 1378
Size: 500 907 2918
Fragments arranged by size:
2918 907 500
PciI A'CATG_T
Cuts at: 2781 2781
Size: 4325
PflMI CCAn_nnn'nTGG
Cuts at: 664 881 1098 664
Size: 217 217 3891
Fragments arranged by size:
3891 217 217
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PmlI CAC'GTG
Cuts at: 249 468 249
Size: 219 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PpuMI rG'GwC_Cy
Cuts at: 586 717 771 1044 586
Size: 131 54 273 3867
Fragments arranged by size:
3867 273 131 54
PstI C_TGCA'G
Cuts at: 215 944 1115 1372 2057 215
Size: 729 171 257 685 2483
Fragments arranged by size:
2483 729 685 257 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 1750 2110 1750
Size: 360 3965
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 1339 1339
Size: 4325
SacII CC_GC'GG
Cuts at: 178 178
Size: 4325
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SanDI GG'GwC_CC
Cuts at: 586 717 586
Size: 131 4194
SapI GCTCTTCn'nnn_
Cuts at: 2348 2558 2348
Size: 210 4115
SbfI CC_TGCA'GG
Cuts at: 1372 1372
Size: 4325
ScaI AGT'ACT
Cuts at: 805 835 3842 805
Size: 30 3007 1288
Fragments arranged by size:
3007 1288 30
SfcI C'TryA_G
Cuts at: 33 211 940 1111 1368 2053 3046 3237 33
Size: 178 729 171 257 685 993 191 1121
Fragments arranged by size:
1121 993 729 685 257 191 178 171
SfiI GGCCn_nnn'nGGCC
Cuts at: 1219 1219
Size: 4325
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4077
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 171 678 1347 171
Size: 507 669 3149
Fragments arranged by size:
3149 669 507
SmlI C'TyrA_G
Cuts at: 776 1123 1480 2395 2887 3149 776
Size: 347 357 915 492 262 1952
Fragments arranged by size:
1952 915 492 357 347 262
SnaBI TAC'GTA
Cuts at: 1317 1317
Size: 4325
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
SrfI GCCC'GGGC
Cuts at: 678 678
Size: 4325
StuI AGG'CCT
Cuts at: 403 403
Size: 4325
StyI C'CwwG_G
Cuts at: 125 1459 1813 125
Size: 1334 354 2637
Fragments arranged by size:
2637 1334 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 315 906 1908 2041 2500 2709 315
Size: 591 1002 133 459 209 1931
Fragments arranged by size:
1931 1002 591 459 209 133
TatI w'GTAC_w
Cuts at: 803 833 944 3840 803
Size: 30 111 2896 1288
Fragments arranged by size:
2896 1288 111 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 553 585 3842 3874 553
Size: 32 3257 32 1004
Fragments arranged by size:
3257 1004 32 32
Tth111I GACn'n_nGTC
Cuts at: 2122 2122
Size: 4325
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 435 747 1820 435
Size: 312 1073 2940
Fragments arranged by size:
2940 1073 312
XhoI C'TCGA_G
Cuts at: 776 776
Size: 4325
XmaI C'CCGG_G
Cuts at: 169 676 1345 169
Size: 507 669 3149
Fragments arranged by size:
3149 669 507
XmnI GAAnn'nnTTC
Cuts at: 934 934
Size: 4325
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PspOMI PspXI PsrI SexAI SgrAI SspI
SwaI ZraI