Restriction map for pF1KSDA0264
 (Circular) (Six-base) MAPSORT of: pF1KSDA0264.seq  Check: 5426  from: 1  to: 4381



pF1KSDA0264  4381 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:33  ..

AccI GT'mk_AC

Cuts at:    230    1417     230 
   Size:       1187    3194
 
Acc65I G'GTAC_C

Cuts at:    431    1395     431 
   Size:        964    3417
 
AclI AA'CG_TT

Cuts at:   4221    4221 
   Size:       4381
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    203     696     983     998    1006    2204    2636    3385    3653 
   Size:        493     287      15       8    1198     432     749     268

Cuts at:   3653     203 
   Size:        931

  Fragments arranged by size: 

                1198     931     749     493     432     287     268      15
                   8
 
AfeI AGC'GCT

Cuts at:    962     962 
   Size:       4381
 
AflIII A'CryG_T

Cuts at:   1731    2837    3814    1731 
   Size:       1106     977    2298

  Fragments arranged by size: 

                2298    1106     977
 
AgeI A'CCGG_T

Cuts at:   2769    2769 
   Size:       4381
 
AlwNI CAG_nnn'CTG

Cuts at:   1260    1684    3253    1260 
   Size:        424    1569    2388

  Fragments arranged by size: 

                2388    1569     424
 
ApaLI G'TGCA_C

Cuts at:   3151    3151 
   Size:       4381
 
ApoI r'AATT_y

Cuts at:    533    1383    3734    3990     533 
   Size:        850    2351     256     924

  Fragments arranged by size: 

                2351     924     850     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4381
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4381
 
AvaI C'yCGr_G

Cuts at:    368    1399     368 
   Size:       1031    3350
 
BamHI G'GATC_C

Cuts at:   1404    1404 
   Size:       4381
 
BanI G'GyrC_C

Cuts at:    431    1395    1809    2057    2092     431 
   Size:        964     414     248      35    2720

  Fragments arranged by size: 

                2720     964     414     248      35
 
BanII G_rGCy'C

Cuts at:    903    1393    2423     903 
   Size:        490    1030    2861

  Fragments arranged by size: 

                2861    1030     490
 
BbeI G_GCGC'C

Cuts at:   1813    2061    1813 
   Size:        248    4133
 
BbsI GAAGACnn'nnnn_

Cuts at:    709     944    1046     709 
   Size:        235     102    4044

  Fragments arranged by size: 

                4044     235     102
 
BbvCI CC'TCA_GC

Cuts at:    182     182 
   Size:       4381
 
BciVI GTATCCnnnnn_n'

Cuts at:   2277    3040    4381    2277 
   Size:        763    1341    2277

  Fragments arranged by size: 

                2277    1341     763
 
BclI T'GATC_A

Cuts at:   1899    1899 
   Size:       4381
 
BglII A'GATC_T

Cuts at:   1894    3933    1894 
   Size:       2039    2342
 
BlpI GC'TnA_GC

Cuts at:   1491    1491 
   Size:       4381
 
Bme1580I G_kGCm'C

Cuts at:   2004    2097    3155    2004 
   Size:         93    1058    3230

  Fragments arranged by size: 

                3230    1058      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    789    1591    1672    2007    4153     789 
   Size:        802      81     335    2146    1017

  Fragments arranged by size: 

                2146    1017     802     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    979    2746     979 
   Size:       1767    2614
 
Bpu10I CC'TnA_GC

Cuts at:    182     182 
   Size:       4381
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1555    2434    2928    3226    1555 
   Size:        879     494     298    2710

  Fragments arranged by size: 

                2710     879     494     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4085    4085 
   Size:       4381
 
BsaAI yAC'GTr

Cuts at:   1371    1732    1371 
   Size:        361    4020
 
BsaBI GATnn'nnATC

Cuts at:      6    1893    3932       6 
   Size:       1887    2039     455

  Fragments arranged by size: 

                2039    1887     455
 
BsaHI Gr'CG_yC

Cuts at:   1810    2058    2762    4258    1810 
   Size:        248     704    1496    1933

  Fragments arranged by size: 

                1933    1496     704     248
 
BsaWI w'CCGG_w

Cuts at:   1568    2089    2769    3043    3190    3601    1568 
   Size:        521     680     274     147     411    2348

  Fragments arranged by size: 

                2348     680     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    739    1033     739 
   Size:        294    4087
 
BseYI C'CCAG_C

Cuts at:   1196    1804    2562    3141    3637    1196 
   Size:        608     758     579     496    1940

  Fragments arranged by size: 

                1940     758     608     579     496
 
BsiEI CG_ry'CG

Cuts at:    104    1921    1967    3177     104 
   Size:       1817      46    1210    1308

  Fragments arranged by size: 

                1817    1308    1210      46
 
BsiHKAI G_wGCw'C

Cuts at:   1393    2171    2361    3155    1393 
   Size:        778     190     794    2619

  Fragments arranged by size: 

                2619     794     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    903    1393    2004    2097    2171    2361    2423    3155     903 
   Size:        490     611      93      74     190      62     732    2129

  Fragments arranged by size: 

                2129     732     611     490     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3601    3601 
   Size:       4381
 
BspHI T'CATG_A

Cuts at:    626    3557     626 
   Size:       2931    1450
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    187     218    1429    1643    1945    2326     187 
   Size:         31    1211     214     302     381    2242

  Fragments arranged by size: 

                2242    1211     381     302     214      31
 
BsrBI CCG'CTC

Cuts at:     50    2671    2725    4211    4376      50 
   Size:       2621      54    1486     165      55

  Fragments arranged by size: 

                2621    1486     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    687    2291     687 
   Size:       1604    2777
 
BsrFI r'CCGG_y

Cuts at:   2377    2558    2769    2377 
   Size:        181     211    3989

  Fragments arranged by size: 

                3989     211     181
 
BsrGI T'GTAC_A

Cuts at:   1284    1284 
   Size:       4381
 
BssSI C'ACGA_G

Cuts at:   2650    3010    2650 
   Size:        360    4021
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1260    1756    1260 
   Size:        496    3885
 
BstBI TT'CG_AA

Cuts at:    110     110 
   Size:       4381
 
BstXI CCAn_nnnn'nTGG

Cuts at:    802     802 
   Size:       4381
 
BstYI r'GATC_y

Cuts at:      1    1404    1871    1894    2229    2475    3489    3575    3587 
   Size:       1403     467      23     335     246    1014      86      12

Cuts at:   3587    3933       1 
   Size:        346     449

  Fragments arranged by size: 

                1403    1014     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    125     125 
   Size:       4381
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1224    2329    2510    2673    1224 
   Size:       1105     181     163    2932

  Fragments arranged by size: 

                2932    1105     181     163
 
BtsI GCAGTG_nn'

Cuts at:    475     986    1273    1622     475 
   Size:        511     287     349    3234

  Fragments arranged by size: 

                3234     511     349     287
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1182    1217    1182 
   Size:         35    4346
 
DraI TTT'AAA

Cuts at:   1378    1378 
   Size:       4381
 
DrdI GACnn_nn'nnGTC

Cuts at:   2085    2945    2085 
   Size:        860    3521
 
EaeI y'GGCC_r

Cuts at:   1964    2138    2529    2556    3903    1964 
   Size:        174     391      27    1347    2442

  Fragments arranged by size: 

                2442    1347     391     174      27
 
EagI C'GGCC_G

Cuts at:   1964    1964 
   Size:       4381
 
EarI CTCTTCn'nnn_

Cuts at:    368     716    2402    2612     368 
   Size:        348    1686     210    2137

  Fragments arranged by size: 

                2137    1686     348     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2587    2899    3045    4191    2587 
   Size:        312     146    1146    2777

  Fragments arranged by size: 

                2777    1146     312     146
 
EcoICRI GAG'CTC

Cuts at:   1391    1391 
   Size:       4381
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    203     696     983     998    1006    2204    2636    3385    3653 
   Size:        493     287      15       8    1198     432     749     268

Cuts at:   3653     203 
   Size:        931

  Fragments arranged by size: 

                1198     931     749     493     432     287     268      15
                   8
 
EcoNI CCTnn'n_nnAGG

Cuts at:    462     462 
   Size:       4381
 
EcoO109I rG'GnC_Cy

Cuts at:   1518    1518 
   Size:       4381
 
EcoRI G'AATT_C

Cuts at:   1383    1383 
   Size:       4381
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    193     726    2398     193 
   Size:        533    1672    2176

  Fragments arranged by size: 

                2176    1672     533
 
FspI TGC'GCA

Cuts at:   2160    2160 
   Size:       4381
 
HaeII r_GCGC'y

Cuts at:    964    1813    2061    3085    4064     964 
   Size:        849     248    1024     979    1281

  Fragments arranged by size: 

                1281    1024     979     849     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    271     303     592     624    1009    1041    2221    2251    2253 
   Size:         32     289      32     385      32    1180      30       2

Cuts at:   2253    2283     271 
   Size:         30    2369

  Fragments arranged by size: 

                2369    1180     385     289      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1418    1418 
   Size:       4381
 
HindIII A'AGCT_T

Cuts at:   1102    1144    1102 
   Size:         42    4339
 
KasI G'GCGC_C

Cuts at:   1809    2057    1809 
   Size:        248    4133
 
KpnI G_GTAC'C

Cuts at:    435    1399     435 
   Size:        964    3417
 
MluI A'CGCG_T

Cuts at:   3814    3814 
   Size:       4381
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3052    3236    3052 
   Size:        184    4197
 
MscI TGG'CCA

Cuts at:   2140    2140 
   Size:       4381
 
MslI CAynn'nnrTG

Cuts at:   2368    2495    2368 
   Size:        127    4254
 
MspA1I CmG'CkG

Cuts at:   1003    1490    1804    2164    3179    3424    1003 
   Size:        487     314     360    1015     245    1960

  Fragments arranged by size: 

                1960    1015     487     360     314     245
 
NaeI GCC'GGC

Cuts at:   2560    2560 
   Size:       4381
 
NarI GG'CG_CC

Cuts at:   1810    2058    1810 
   Size:        248    4133
 
NcoI C'CATG_G

Cuts at:    125     125 
   Size:       4381
 
NgoMIV G'CCGG_C

Cuts at:   2558    2558 
   Size:       4381
 
NruI TCG'CGA

Cuts at:   2742    2742 
   Size:       4381
 
NspI r_CATG'y

Cuts at:    867    1432    1932    2841     867 
   Size:        565     500     909    2407

  Fragments arranged by size: 

                2407     909     565     500
 
PciI A'CATG_T

Cuts at:   2837    2837 
   Size:       4381
 
PflMI CCAn_nnn'nTGG

Cuts at:    428     428 
   Size:       4381
 
PmeI GTTT'AAAC

Cuts at:   1378    1378 
   Size:       4381
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3520    3552    3520 
   Size:         32    4349
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    506     538     506 
   Size:         32    4349
 
PstI C_TGCA'G

Cuts at:    198    1265    1426    2111     198 
   Size:       1067     161     685    2468

  Fragments arranged by size: 

                2468    1067     685     161
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4381
 
PvuII CAG'CTG

Cuts at:   1003    1804    2164    1003 
   Size:        801     360    3220

  Fragments arranged by size: 

                3220     801     360
 
RsrII CG'GwC_CG

Cuts at:   2574    2574 
   Size:       4381
 
SacI G_AGCT'C

Cuts at:   1393    1393 
   Size:       4381
 
SalI G'TCGA_C

Cuts at:   1416    1416 
   Size:       4381
 
SapI GCTCTTCn'nnn_

Cuts at:   2402    2612    2402 
   Size:        210    4171
 
SbfI CC_TGCA'GG

Cuts at:    198    1426     198 
   Size:       1228    3153
 
ScaI AGT'ACT

Cuts at:   3898    3898 
   Size:       4381
 
SfcI C'TryA_G

Cuts at:     33     194    1261    1422    2107    3102    3293      33 
   Size:        161    1067     161     685     995     191    1121

  Fragments arranged by size: 

                1121    1067     995     685     191     161     161
 
SfoI GGC'GCC

Cuts at:   1811    2059    1811 
   Size:        248    4133
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4381
 
SmaI CCC'GGG

Cuts at:   1401    1401 
   Size:       4381
 
SmlI C'TyrA_G

Cuts at:   1534    2449    2943    3205    1534 
   Size:        915     494     262    2710

  Fragments arranged by size: 

                2710     915     494     262
 
SnaBI TAC'GTA

Cuts at:   1371    1371 
   Size:       4381
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4381
 
SphI G_CATG'C

Cuts at:   1432    1932    1432 
   Size:        500    3881
 
StuI AGG'CCT

Cuts at:    761    1132     761 
   Size:        371    4010
 
StyI C'CwwG_G

Cuts at:    125    1513    1867     125 
   Size:       1388     354    2639

  Fragments arranged by size: 

                2639    1388     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    673    1210    1962    2095    2554    2765     673 
   Size:        537     752     133     459     211    2289

  Fragments arranged by size: 

                2289     752     537     459     211     133
 
TatI w'GTAC_w

Cuts at:   1284    3896    1284 
   Size:       2612    1769
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    723     755    3898    3930     723 
   Size:         32    3143      32    1174

  Fragments arranged by size: 

                3143    1174      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2176    2176 
   Size:       4381
 
XbaI T'CTAG_A

Cuts at:   1275    1410    1275 
   Size:        135    4246
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1874    1874 
   Size:       4381
 
XmaI C'CCGG_G

Cuts at:   1399    1399 
   Size:       4381
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NruI     NspI     PciI    PflMI     PmeI     PpiI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI
     BpmI    BsaXI     BsgI    BsiWI     BsmI    BsmBI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI   DraIII    EcoRV     FseI    FspAI     HpaI
     MfeI     NdeI     NheI     NotI     NsiI     PacI     PasI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI    SacII    SanDI
    SexAI     SfiI    SgrAI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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