(Circular) (Six-base) MAPSORT of: pF1KSDA0264.seq Check: 5426 from: 1 to: 4381 pF1KSDA0264 4381 bp With 182 enzymes: SgfI * June 20, 2008 10:33 .. AccI GT'mk_AC Cuts at: 230 1417 230 Size: 1187 3194 Acc65I G'GTAC_C Cuts at: 431 1395 431 Size: 964 3417 AclI AA'CG_TT Cuts at: 4221 4221 Size: 4381 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 203 696 983 998 1006 2204 2636 3385 3653 Size: 493 287 15 8 1198 432 749 268 Cuts at: 3653 203 Size: 931 Fragments arranged by size: 1198 931 749 493 432 287 268 15 8 AfeI AGC'GCT Cuts at: 962 962 Size: 4381 AflIII A'CryG_T Cuts at: 1731 2837 3814 1731 Size: 1106 977 2298 Fragments arranged by size: 2298 1106 977 AgeI A'CCGG_T Cuts at: 2769 2769 Size: 4381 AlwNI CAG_nnn'CTG Cuts at: 1260 1684 3253 1260 Size: 424 1569 2388 Fragments arranged by size: 2388 1569 424 ApaLI G'TGCA_C Cuts at: 3151 3151 Size: 4381 ApoI r'AATT_y Cuts at: 533 1383 3734 3990 533 Size: 850 2351 256 924 Fragments arranged by size: 2351 924 850 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4381 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4381 AvaI C'yCGr_G Cuts at: 368 1399 368 Size: 1031 3350 BamHI G'GATC_C Cuts at: 1404 1404 Size: 4381 BanI G'GyrC_C Cuts at: 431 1395 1809 2057 2092 431 Size: 964 414 248 35 2720 Fragments arranged by size: 2720 964 414 248 35 BanII G_rGCy'C Cuts at: 903 1393 2423 903 Size: 490 1030 2861 Fragments arranged by size: 2861 1030 490 BbeI G_GCGC'C Cuts at: 1813 2061 1813 Size: 248 4133 BbsI GAAGACnn'nnnn_ Cuts at: 709 944 1046 709 Size: 235 102 4044 Fragments arranged by size: 4044 235 102 BbvCI CC'TCA_GC Cuts at: 182 182 Size: 4381 BciVI GTATCCnnnnn_n' Cuts at: 2277 3040 4381 2277 Size: 763 1341 2277 Fragments arranged by size: 2277 1341 763 BclI T'GATC_A Cuts at: 1899 1899 Size: 4381 BglII A'GATC_T Cuts at: 1894 3933 1894 Size: 2039 2342 BlpI GC'TnA_GC Cuts at: 1491 1491 Size: 4381 Bme1580I G_kGCm'C Cuts at: 2004 2097 3155 2004 Size: 93 1058 3230 Fragments arranged by size: 3230 1058 93 BmrI ACTGGGnnnn_n' Cuts at: 789 1591 1672 2007 4153 789 Size: 802 81 335 2146 1017 Fragments arranged by size: 2146 1017 802 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 979 2746 979 Size: 1767 2614 Bpu10I CC'TnA_GC Cuts at: 182 182 Size: 4381 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1555 2434 2928 3226 1555 Size: 879 494 298 2710 Fragments arranged by size: 2710 879 494 298 BsaI GGTCTCn'nnnn_ Cuts at: 4085 4085 Size: 4381 BsaAI yAC'GTr Cuts at: 1371 1732 1371 Size: 361 4020 BsaBI GATnn'nnATC Cuts at: 6 1893 3932 6 Size: 1887 2039 455 Fragments arranged by size: 2039 1887 455 BsaHI Gr'CG_yC Cuts at: 1810 2058 2762 4258 1810 Size: 248 704 1496 1933 Fragments arranged by size: 1933 1496 704 248 BsaWI w'CCGG_w Cuts at: 1568 2089 2769 3043 3190 3601 1568 Size: 521 680 274 147 411 2348 Fragments arranged by size: 2348 680 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 739 1033 739 Size: 294 4087 BseYI C'CCAG_C Cuts at: 1196 1804 2562 3141 3637 1196 Size: 608 758 579 496 1940 Fragments arranged by size: 1940 758 608 579 496 BsiEI CG_ry'CG Cuts at: 104 1921 1967 3177 104 Size: 1817 46 1210 1308 Fragments arranged by size: 1817 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 1393 2171 2361 3155 1393 Size: 778 190 794 2619 Fragments arranged by size: 2619 794 778 190 Bsp1286I G_dGCh'C Cuts at: 903 1393 2004 2097 2171 2361 2423 3155 903 Size: 490 611 93 74 190 62 732 2129 Fragments arranged by size: 2129 732 611 490 190 93 74 62 BspEI T'CCGG_A Cuts at: 3601 3601 Size: 4381 BspHI T'CATG_A Cuts at: 626 3557 626 Size: 2931 1450 BspMI ACCTGCnnnn'nnnn_ Cuts at: 187 218 1429 1643 1945 2326 187 Size: 31 1211 214 302 381 2242 Fragments arranged by size: 2242 1211 381 302 214 31 BsrBI CCG'CTC Cuts at: 50 2671 2725 4211 4376 50 Size: 2621 54 1486 165 55 Fragments arranged by size: 2621 1486 165 55 54 BsrDI GCAATG_nn' Cuts at: 687 2291 687 Size: 1604 2777 BsrFI r'CCGG_y Cuts at: 2377 2558 2769 2377 Size: 181 211 3989 Fragments arranged by size: 3989 211 181 BsrGI T'GTAC_A Cuts at: 1284 1284 Size: 4381 BssSI C'ACGA_G Cuts at: 2650 3010 2650 Size: 360 4021 BstAPI GCAn_nnn'nTGC Cuts at: 1260 1756 1260 Size: 496 3885 BstBI TT'CG_AA Cuts at: 110 110 Size: 4381 BstXI CCAn_nnnn'nTGG Cuts at: 802 802 Size: 4381 BstYI r'GATC_y Cuts at: 1 1404 1871 1894 2229 2475 3489 3575 3587 Size: 1403 467 23 335 246 1014 86 12 Cuts at: 3587 3933 1 Size: 346 449 Fragments arranged by size: 1403 1014 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 125 125 Size: 4381 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1224 2329 2510 2673 1224 Size: 1105 181 163 2932 Fragments arranged by size: 2932 1105 181 163 BtsI GCAGTG_nn' Cuts at: 475 986 1273 1622 475 Size: 511 287 349 3234 Fragments arranged by size: 3234 511 349 287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1182 1217 1182 Size: 35 4346 DraI TTT'AAA Cuts at: 1378 1378 Size: 4381 DrdI GACnn_nn'nnGTC Cuts at: 2085 2945 2085 Size: 860 3521 EaeI y'GGCC_r Cuts at: 1964 2138 2529 2556 3903 1964 Size: 174 391 27 1347 2442 Fragments arranged by size: 2442 1347 391 174 27 EagI C'GGCC_G Cuts at: 1964 1964 Size: 4381 EarI CTCTTCn'nnn_ Cuts at: 368 716 2402 2612 368 Size: 348 1686 210 2137 Fragments arranged by size: 2137 1686 348 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2587 2899 3045 4191 2587 Size: 312 146 1146 2777 Fragments arranged by size: 2777 1146 312 146 EcoICRI GAG'CTC Cuts at: 1391 1391 Size: 4381 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 203 696 983 998 1006 2204 2636 3385 3653 Size: 493 287 15 8 1198 432 749 268 Cuts at: 3653 203 Size: 931 Fragments arranged by size: 1198 931 749 493 432 287 268 15 8 EcoNI CCTnn'n_nnAGG Cuts at: 462 462 Size: 4381 EcoO109I rG'GnC_Cy Cuts at: 1518 1518 Size: 4381 EcoRI G'AATT_C Cuts at: 1383 1383 Size: 4381 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 193 726 2398 193 Size: 533 1672 2176 Fragments arranged by size: 2176 1672 533 FspI TGC'GCA Cuts at: 2160 2160 Size: 4381 HaeII r_GCGC'y Cuts at: 964 1813 2061 3085 4064 964 Size: 849 248 1024 979 1281 Fragments arranged by size: 1281 1024 979 849 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 271 303 592 624 1009 1041 2221 2251 2253 Size: 32 289 32 385 32 1180 30 2 Cuts at: 2253 2283 271 Size: 30 2369 Fragments arranged by size: 2369 1180 385 289 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1418 1418 Size: 4381 HindIII A'AGCT_T Cuts at: 1102 1144 1102 Size: 42 4339 KasI G'GCGC_C Cuts at: 1809 2057 1809 Size: 248 4133 KpnI G_GTAC'C Cuts at: 435 1399 435 Size: 964 3417 MluI A'CGCG_T Cuts at: 3814 3814 Size: 4381 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3052 3236 3052 Size: 184 4197 MscI TGG'CCA Cuts at: 2140 2140 Size: 4381 MslI CAynn'nnrTG Cuts at: 2368 2495 2368 Size: 127 4254 MspA1I CmG'CkG Cuts at: 1003 1490 1804 2164 3179 3424 1003 Size: 487 314 360 1015 245 1960 Fragments arranged by size: 1960 1015 487 360 314 245 NaeI GCC'GGC Cuts at: 2560 2560 Size: 4381 NarI GG'CG_CC Cuts at: 1810 2058 1810 Size: 248 4133 NcoI C'CATG_G Cuts at: 125 125 Size: 4381 NgoMIV G'CCGG_C Cuts at: 2558 2558 Size: 4381 NruI TCG'CGA Cuts at: 2742 2742 Size: 4381 NspI r_CATG'y Cuts at: 867 1432 1932 2841 867 Size: 565 500 909 2407 Fragments arranged by size: 2407 909 565 500 PciI A'CATG_T Cuts at: 2837 2837 Size: 4381 PflMI CCAn_nnn'nTGG Cuts at: 428 428 Size: 4381 PmeI GTTT'AAAC Cuts at: 1378 1378 Size: 4381 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3520 3552 3520 Size: 32 4349 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 506 538 506 Size: 32 4349 PstI C_TGCA'G Cuts at: 198 1265 1426 2111 198 Size: 1067 161 685 2468 Fragments arranged by size: 2468 1067 685 161 PvuI CG_AT'CG Cuts at: 104 104 Size: 4381 PvuII CAG'CTG Cuts at: 1003 1804 2164 1003 Size: 801 360 3220 Fragments arranged by size: 3220 801 360 RsrII CG'GwC_CG Cuts at: 2574 2574 Size: 4381 SacI G_AGCT'C Cuts at: 1393 1393 Size: 4381 SalI G'TCGA_C Cuts at: 1416 1416 Size: 4381 SapI GCTCTTCn'nnn_ Cuts at: 2402 2612 2402 Size: 210 4171 SbfI CC_TGCA'GG Cuts at: 198 1426 198 Size: 1228 3153 ScaI AGT'ACT Cuts at: 3898 3898 Size: 4381 SfcI C'TryA_G Cuts at: 33 194 1261 1422 2107 3102 3293 33 Size: 161 1067 161 685 995 191 1121 Fragments arranged by size: 1121 1067 995 685 191 161 161 SfoI GGC'GCC Cuts at: 1811 2059 1811 Size: 248 4133 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4381 SmaI CCC'GGG Cuts at: 1401 1401 Size: 4381 SmlI C'TyrA_G Cuts at: 1534 2449 2943 3205 1534 Size: 915 494 262 2710 Fragments arranged by size: 2710 915 494 262 SnaBI TAC'GTA Cuts at: 1371 1371 Size: 4381 SpeI A'CTAG_T Cuts at: 67 67 Size: 4381 SphI G_CATG'C Cuts at: 1432 1932 1432 Size: 500 3881 StuI AGG'CCT Cuts at: 761 1132 761 Size: 371 4010 StyI C'CwwG_G Cuts at: 125 1513 1867 125 Size: 1388 354 2639 Fragments arranged by size: 2639 1388 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 673 1210 1962 2095 2554 2765 673 Size: 537 752 133 459 211 2289 Fragments arranged by size: 2289 752 537 459 211 133 TatI w'GTAC_w Cuts at: 1284 3896 1284 Size: 2612 1769 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 723 755 3898 3930 723 Size: 32 3143 32 1174 Fragments arranged by size: 3143 1174 32 32 Tth111I GACn'n_nGTC Cuts at: 2176 2176 Size: 4381 XbaI T'CTAG_A Cuts at: 1275 1410 1275 Size: 135 4246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1874 1874 Size: 4381 XmaI C'CCGG_G Cuts at: 1399 1399 Size: 4381 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI PflMI PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI