Restriction map for
pF1KSDA0251
(Circular) (Six-base) MAPSORT of: pF1KSDA0251.seq Check: 1266 from: 1 to: 5503
pF1KSDA0251 5503 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2159 2539 2159
Size: 380 5123
Acc65I G'GTAC_C
Cuts at: 2517 2517
Size: 5503
AclI AA'CG_TT
Cuts at: 5343 5343
Size: 5503
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1355 1453 1792 3326 3758 4507 4775 1355
Size: 98 339 1534 432 749 268 2083
Fragments arranged by size:
2083 1534 749 432 339 268 98
AflII C'TTAA_G
Cuts at: 2484 2484
Size: 5503
AflIII A'CryG_T
Cuts at: 131 1489 2853 3959 4936 131
Size: 1358 1364 1106 977 698
Fragments arranged by size:
1364 1358 1106 977 698
AgeI A'CCGG_T
Cuts at: 3891 3891
Size: 5503
AleI CACnn'nnGTG
Cuts at: 1397 1397
Size: 5503
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1510 1510
Size: 5503
AlwNI CAG_nnn'CTG
Cuts at: 2806 4375 2806
Size: 1569 3934
ApaLI G'TGCA_C
Cuts at: 4273 4273
Size: 5503
ApoI r'AATT_y
Cuts at: 506 2505 4856 5112 506
Size: 1999 2351 256 897
Fragments arranged by size:
2351 1999 897 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5503
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5503
AvaI C'yCGr_G
Cuts at: 1535 1913 1932 2202 2356 2521 1535
Size: 378 19 270 154 165 4517
Fragments arranged by size:
4517 378 270 165 154 19
BamHI G'GATC_C
Cuts at: 757 2526 757
Size: 1769 3734
BanI G'GyrC_C
Cuts at: 1443 2517 2931 3179 3214 1443
Size: 1074 414 248 35 3732
Fragments arranged by size:
3732 1074 414 248 35
BanII G_rGCy'C
Cuts at: 356 364 1280 1889 2062 2391 2515 3545 356
Size: 8 916 609 173 329 124 1030 2314
Fragments arranged by size:
2314 1030 916 609 329 173 124 8
BbeI G_GCGC'C
Cuts at: 2935 3183 2935
Size: 248 5255
BbsI GAAGACnn'nnnn_
Cuts at: 608 828 2312 608
Size: 220 1484 3799
Fragments arranged by size:
3799 1484 220
BbvCI CC'TCA_GC
Cuts at: 1481 2399 1481
Size: 918 4585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1063 1097 1063
Size: 34 5469
BciVI GTATCCnnnnn_n'
Cuts at: 467 3399 4162 5503 467
Size: 2932 763 1341 467
Fragments arranged by size:
2932 1341 763 467
BclI T'GATC_A
Cuts at: 3021 3021
Size: 5503
BfrBI ATG'CAT
Cuts at: 333 333
Size: 5503
BglII A'GATC_T
Cuts at: 3016 5055 3016
Size: 2039 3464
BlpI GC'TnA_GC
Cuts at: 1279 1883 2613 1279
Size: 604 730 4169
Fragments arranged by size:
4169 730 604
Bme1580I G_kGCm'C
Cuts at: 1140 1358 1489 3126 3219 4277 1140
Size: 218 131 1637 93 1058 2366
Fragments arranged by size:
2366 1637 1058 218 131 93
BmrI ACTGGGnnnn_n'
Cuts at: 1282 1345 2713 2794 3129 5275 1282
Size: 63 1368 81 335 2146 1510
Fragments arranged by size:
2146 1510 1368 335 81 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1299 1410 1947 2304 3868 1299
Size: 111 537 357 1564 2934
Fragments arranged by size:
2934 1564 537 357 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 226 262 295 808 1588 2155 160
Size: 66 36 33 513 780 567 3508
Fragments arranged by size:
3508 780 567 513 66 36 33
Bpu10I CC'TnA_GC
Cuts at: 1481 2399 1481
Size: 918 4585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1970 2677 3556 4050 4348 1970
Size: 707 879 494 298 3125
Fragments arranged by size:
3125 879 707 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 1654 1888 2275 5207 1654
Size: 234 387 2932 1950
Fragments arranged by size:
2932 1950 387 234
BsaAI yAC'GTr
Cuts at: 1490 2493 2854 1490
Size: 1003 361 4139
Fragments arranged by size:
4139 1003 361
BsaBI GATnn'nnATC
Cuts at: 6 1550 3015 5054 6
Size: 1544 1465 2039 455
Fragments arranged by size:
2039 1544 1465 455
BsaHI Gr'CG_yC
Cuts at: 2932 3180 3884 5380 2932
Size: 248 704 1496 3055
Fragments arranged by size:
3055 1496 704 248
BsaWI w'CCGG_w
Cuts at: 256 1332 2082 2475 2690 3211 3891 4165 4312
Size: 1076 750 393 215 521 680 274 147
Cuts at: 4312 4723 256
Size: 411 1036
Fragments arranged by size:
1076 1036 750 680 521 411 393 274
215 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1002 1032 1532 1562 2175 2205 1002
Size: 30 500 30 613 30 4300
Fragments arranged by size:
4300 613 500 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 963 1939 364
Size: 599 976 3928
Fragments arranged by size:
3928 976 599
BseYI C'CCAG_C
Cuts at: 761 1032 1419 2392 2926 3684 4263 4759 761
Size: 271 387 973 534 758 579 496 1505
Fragments arranged by size:
1505 973 758 579 534 496 387 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 2195
Size: 5503
BsiEI CG_ry'CG
Cuts at: 104 1383 3043 3089 4299 104
Size: 1279 1660 46 1210 1308
Fragments arranged by size:
1660 1308 1279 1210 46
BsiHKAI G_wGCw'C
Cuts at: 1280 1889 2515 3293 3483 4277 1280
Size: 609 626 778 190 794 2506
Fragments arranged by size:
2506 794 778 626 609 190
BsmI GAATG_Cn'
Cuts at: 1973 1973
Size: 5503
Bsp1286I G_dGCh'C
Cuts at: 356 364 1140 1280 1358 1489 1889 2062 2391
Size: 8 776 140 78 131 400 173 329
Cuts at: 2391 2515 3126 3219 3293 3483 3545 4277 356
Size: 124 611 93 74 190 62 732 1582
Fragments arranged by size:
1582 776 732 611 400 329 190 173
140 131 124 93 78 74 62 8
BspEI T'CCGG_A
Cuts at: 256 4723 256
Size: 4467 1036
BspHI T'CATG_A
Cuts at: 4679 4679
Size: 5503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1636 2551 2765 3067 3448 1636
Size: 915 214 302 381 3691
Fragments arranged by size:
3691 915 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3793 3847 5333 5498 50
Size: 3743 54 1486 165 55
Fragments arranged by size:
3743 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1898 3413 1898
Size: 1515 3988
BsrFI r'CCGG_y
Cuts at: 3499 3680 3891 3499
Size: 181 211 5111
Fragments arranged by size:
5111 211 181
BssSI C'ACGA_G
Cuts at: 1395 1888 3772 4132 1395
Size: 493 1884 360 2766
Fragments arranged by size:
2766 1884 493 360
BstAPI GCAn_nnn'nTGC
Cuts at: 1443 2173 2878 1443
Size: 730 705 4068
Fragments arranged by size:
4068 730 705
BstBI TT'CG_AA
Cuts at: 110 110
Size: 5503
BstXI CCAn_nnnn'nTGG
Cuts at: 286 379 286
Size: 93 5410
BstYI r'GATC_y
Cuts at: 1 757 1329 1470 1737 2045 2526 2993 3016
Size: 756 572 141 267 308 481 467 23
Cuts at: 3016 3351 3597 4611 4697 4709 5055 1
Size: 335 246 1014 86 12 346 449
Fragments arranged by size:
1014 756 572 481 467 449 346 335
308 267 246 141 86 23 12
BtgI C'CryG_G
Cuts at: 125 1027 125
Size: 902 4601
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3451 3632 3795 3451
Size: 181 163 5159
Fragments arranged by size:
5159 181 163
BtsI GCAGTG_nn'
Cuts at: 200 291 2744 200
Size: 91 2453 2959
Fragments arranged by size:
2959 2453 91
DraI TTT'AAA
Cuts at: 1341 1809 2247 2500 1341
Size: 468 438 253 4344
Fragments arranged by size:
4344 468 438 253
DraIII CAC_nnn'GTG
Cuts at: 2173 2173
Size: 5503
DrdI GACnn_nn'nnGTC
Cuts at: 3207 4067 3207
Size: 860 4643
EaeI y'GGCC_r
Cuts at: 1380 1904 3086 3260 3651 3678 5025 1380
Size: 524 1182 174 391 27 1347 1858
Fragments arranged by size:
1858 1347 1182 524 391 174 27
EagI C'GGCC_G
Cuts at: 1380 3086 1380
Size: 1706 3797
EarI CTCTTCn'nnn_
Cuts at: 224 539 1610 1831 2021 3524 3734 224
Size: 315 1071 221 190 1503 210 1993
Fragments arranged by size:
1993 1503 1071 315 221 210 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3709 4021 4167 5313 3709
Size: 312 146 1146 3899
Fragments arranged by size:
3899 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1278 1887 2513 1278
Size: 609 626 4268
Fragments arranged by size:
4268 626 609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 226 262 295 808 1355 1453 1588 1792
Size: 66 36 33 513 547 98 135 204
Cuts at: 1792 2155 3326 3758 4507 4775 160
Size: 363 1171 432 749 268 888
Fragments arranged by size:
1171 888 749 547 513 432 363 268
204 135 98 66 36 33
EcoO109I rG'GnC_Cy
Cuts at: 1816 2252 2436 2640 1816
Size: 436 184 204 4679
Fragments arranged by size:
4679 436 204 184
EcoRI G'AATT_C
Cuts at: 2505 2505
Size: 5503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 3520 2035
Size: 1485 4018
FspI TGC'GCA
Cuts at: 3282 3282
Size: 5503
HaeII r_GCGC'y
Cuts at: 2328 2935 3183 4207 5186 2328
Size: 607 248 1024 979 2645
Fragments arranged by size:
2645 1024 979 607 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1002 1034 2314 2346 2443 2475 3343 3373 3375
Size: 32 1280 32 97 32 868 30 2
Cuts at: 3375 3405 1002
Size: 30 3100
Fragments arranged by size:
3100 1280 868 97 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1210 1219 2540 1210
Size: 9 1321 4173
Fragments arranged by size:
4173 1321 9
KasI G'GCGC_C
Cuts at: 2931 3179 2931
Size: 248 5255
KpnI G_GTAC'C
Cuts at: 2521 2521
Size: 5503
MluI A'CGCG_T
Cuts at: 131 4936 131
Size: 4805 698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2218 4174 4358 2218
Size: 1956 184 3363
Fragments arranged by size:
3363 1956 184
MscI TGG'CCA
Cuts at: 3262 3262
Size: 5503
MslI CAynn'nnrTG
Cuts at: 338 1012 1397 1465 1709 3490 3617 338
Size: 674 385 68 244 1781 127 2224
Fragments arranged by size:
2224 1781 674 385 244 127 68
MspA1I CmG'CkG
Cuts at: 1045 1431 1440 2612 2926 3286 4301 4546 1045
Size: 386 9 1172 314 360 1015 245 2002
Fragments arranged by size:
2002 1172 1015 386 360 314 245 9
NaeI GCC'GGC
Cuts at: 3682 3682
Size: 5503
NarI GG'CG_CC
Cuts at: 2932 3180 2932
Size: 248 5255
NcoI C'CATG_G
Cuts at: 125 125
Size: 5503
NdeI CA'TA_TG
Cuts at: 395 919 395
Size: 524 4979
NgoMIV G'CCGG_C
Cuts at: 3680 3680
Size: 5503
NruI TCG'CGA
Cuts at: 3864 3864
Size: 5503
NsiI A_TGCA'T
Cuts at: 335 335
Size: 5503
NspI r_CATG'y
Cuts at: 1204 1708 2554 3054 3963 1204
Size: 504 846 500 909 2744
Fragments arranged by size:
2744 909 846 504 500
PasI CC'CwG_GG
Cuts at: 2383 2383
Size: 5503
PciI A'CATG_T
Cuts at: 3959 3959
Size: 5503
PflMI CCAn_nnn'nTGG
Cuts at: 494 494
Size: 5503
PfoI T'CCnGG_A
Cuts at: 785 1310 785
Size: 525 4978
PmeI GTTT'AAAC
Cuts at: 2500 2500
Size: 5503
PmlI CAC'GTG
Cuts at: 1490 1490
Size: 5503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4642 4674 4642
Size: 32 5471
PpuMI rG'GwC_Cy
Cuts at: 2252 2436 2252
Size: 184 5319
PspXI vC'TCGA_Gb
Cuts at: 1932 2356 1932
Size: 424 5079
PstI C_TGCA'G
Cuts at: 993 2000 2548 3233 993
Size: 1007 548 685 3263
Fragments arranged by size:
3263 1007 685 548
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5503
PvuII CAG'CTG
Cuts at: 1045 1431 1440 2926 3286 1045
Size: 386 9 1486 360 3262
Fragments arranged by size:
3262 1486 386 360 9
RsrII CG'GwC_CG
Cuts at: 3696 3696
Size: 5503
SacI G_AGCT'C
Cuts at: 1280 1889 2515 1280
Size: 609 626 4268
Fragments arranged by size:
4268 626 609
SalI G'TCGA_C
Cuts at: 2538 2538
Size: 5503
SanDI GG'GwC_CC
Cuts at: 2436 2436
Size: 5503
SapI GCTCTTCn'nnn_
Cuts at: 1831 3524 3734 1831
Size: 1693 210 3600
Fragments arranged by size:
3600 1693 210
SbfI CC_TGCA'GG
Cuts at: 2548 2548
Size: 5503
ScaI AGT'ACT
Cuts at: 5020 5020
Size: 5503
SfcI C'TryA_G
Cuts at: 33 989 1996 2051 2544 3229 4224 4415 33
Size: 956 1007 55 493 685 995 191 1121
Fragments arranged by size:
1121 1007 995 956 685 493 191 55
SfoI GGC'GCC
Cuts at: 2933 3181 2933
Size: 248 5255
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5503
SmaI CCC'GGG
Cuts at: 1915 2523 1915
Size: 608 4895
SmlI C'TyrA_G
Cuts at: 1932 1985 2356 2484 2656 3571 4065 4327 1932
Size: 53 371 128 172 915 494 262 3108
Fragments arranged by size:
3108 915 494 371 262 172 128 53
SnaBI TAC'GTA
Cuts at: 2493 2493
Size: 5503
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5503
SphI G_CATG'C
Cuts at: 1204 2554 3054 1204
Size: 1350 500 3653
Fragments arranged by size:
3653 1350 500
SspI AAT'ATT
Cuts at: 378 378
Size: 5503
StuI AGG'CCT
Cuts at: 2422 2422
Size: 5503
StyI C'CwwG_G
Cuts at: 125 2635 2989 125
Size: 2510 354 2639
Fragments arranged by size:
2639 2510 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 515 2396 2431 2459 3084 3217 3676 3887 515
Size: 1881 35 28 625 133 459 211 2131
Fragments arranged by size:
2131 1881 625 459 211 133 35 28
TatI w'GTAC_w
Cuts at: 5018 5018
Size: 5503
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1532 1564 2175 2207 5020 5052 1532
Size: 32 611 32 2813 32 1983
Fragments arranged by size:
2813 1983 611 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 413 1870 3298 413
Size: 1457 1428 2618
Fragments arranged by size:
2618 1457 1428
XbaI T'CTAG_A
Cuts at: 2532 2532
Size: 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2996 2996
Size: 5503
XhoI C'TCGA_G
Cuts at: 1932 2356 1932
Size: 424 5079
XmaI C'CCGG_G
Cuts at: 1913 2521 1913
Size: 608 4895
XmnI GAAnn'nnTTC
Cuts at: 1961 2018 1961
Size: 57 5446
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AloI ApaI AscI AvrII
BaeI BglI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI PacI PshAI
PsiI PspOMI PsrI SacII SexAI SfiI SgrAI SrfI
SwaI ZraI