Restriction map for
pF1KSDA0250
(Circular) (Six-base) MAPSORT of: pF1KSDA0250.seq Check: 7711 from: 1 to: 6412
pF1KSDA0250 6412 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2251 3448 2251
Size: 1197 5215
Acc65I G'GTAC_C
Cuts at: 3426 3426
Size: 6412
AclI AA'CG_TT
Cuts at: 6252 6252
Size: 6412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 227 1889 2059 2437 4235 4667 5416 5684
Size: 37 1662 170 378 1798 432 749 268
Cuts at: 5684 190
Size: 918
Fragments arranged by size:
1798 1662 918 749 432 378 268 170
37
AflIII A'CryG_T
Cuts at: 3762 4868 5845 3762
Size: 1106 977 4329
Fragments arranged by size:
4329 1106 977
AgeI A'CCGG_T
Cuts at: 4800 4800
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 1252 1252
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1049 1049
Size: 6412
AlwNI CAG_nnn'CTG
Cuts at: 231 240 1119 1867 3715 5284 231
Size: 9 879 748 1848 1569 1359
Fragments arranged by size:
1848 1569 1359 879 748 9
ApaLI G'TGCA_C
Cuts at: 5182 5182
Size: 6412
ApoI r'AATT_y
Cuts at: 889 1719 2590 3414 5765 6021 889
Size: 830 871 824 2351 256 1280
Fragments arranged by size:
2351 1280 871 830 824 256
AseI AT'TA_AT
Cuts at: 20 1600 20
Size: 1580 4832
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6412
AvaI C'yCGr_G
Cuts at: 3430 3430
Size: 6412
BamHI G'GATC_C
Cuts at: 2703 3435 2703
Size: 732 5680
BanI G'GyrC_C
Cuts at: 3223 3379 3426 3840 4088 4123 3223
Size: 156 47 414 248 35 5512
Fragments arranged by size:
5512 414 248 156 47 35
BanII G_rGCy'C
Cuts at: 937 1687 2029 2759 3424 4454 937
Size: 750 342 730 665 1030 2895
Fragments arranged by size:
2895 1030 750 730 665 342
BbeI G_GCGC'C
Cuts at: 3844 4092 3844
Size: 248 6164
BbsI GAAGACnn'nnnn_
Cuts at: 485 1616 485
Size: 1131 5281
BciVI GTATCCnnnnn_n'
Cuts at: 4308 5071 6412 4308
Size: 763 1341 4308
Fragments arranged by size:
4308 1341 763
BclI T'GATC_A
Cuts at: 3930 3930
Size: 6412
BfrBI ATG'CAT
Cuts at: 3312 3312
Size: 6412
BglII A'GATC_T
Cuts at: 2629 3925 5964 2629
Size: 1296 2039 3077
Fragments arranged by size:
3077 2039 1296
BlpI GC'TnA_GC
Cuts at: 662 2234 3522 662
Size: 1572 1288 3552
Fragments arranged by size:
3552 1572 1288
Bme1580I G_kGCm'C
Cuts at: 4035 4128 5186 4035
Size: 93 1058 5261
Fragments arranged by size:
5261 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 1610 1914 2607 3622 3703 4038 6184 1610
Size: 304 693 1015 81 335 2146 1838
Fragments arranged by size:
2146 1838 1015 693 335 304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1362 2766 3276 4777 1362
Size: 1404 510 1501 2997
Fragments arranged by size:
2997 1501 1404 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1844 1964 2078 3074 3352 1844
Size: 120 114 996 278 4904
Fragments arranged by size:
4904 996 278 120 114
Bpu10I CC'TnA_GC
Cuts at: 2028 2028
Size: 6412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1332 1889 3586 4465 4959 5257 1332
Size: 557 1697 879 494 298 2487
Fragments arranged by size:
2487 1697 879 557 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 719 1227 1401 1793 2951 6116 719
Size: 508 174 392 1158 3165 1015
Fragments arranged by size:
3165 1158 1015 508 392 174
BsaAI yAC'GTr
Cuts at: 3402 3763 3402
Size: 361 6051
BsaBI GATnn'nnATC
Cuts at: 6 3924 5963 6
Size: 3918 2039 455
Fragments arranged by size:
3918 2039 455
BsaHI Gr'CG_yC
Cuts at: 3841 4089 4793 6289 3841
Size: 248 704 1496 3964
Fragments arranged by size:
3964 1496 704 248
BsaWI w'CCGG_w
Cuts at: 2791 3599 4120 4800 5074 5221 5632 2791
Size: 808 521 680 274 147 411 3571
Fragments arranged by size:
3571 808 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3325 3355 3325
Size: 30 6382
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1198 1390 1946 2987 3023 1198
Size: 192 556 1041 36 4587
Fragments arranged by size:
4587 1041 556 192 36
BseYI C'CCAG_C
Cuts at: 195 783 1963 2043 2116 2207 3025 3835 4593
Size: 588 1180 80 73 91 818 810 758
Cuts at: 4593 5172 5668 195
Size: 579 496 939
Fragments arranged by size:
1180 939 818 810 758 588 579 496
91 80 73
BsiEI CG_ry'CG
Cuts at: 104 3952 3998 5208 104
Size: 3848 46 1210 1308
Fragments arranged by size:
3848 1308 1210 46
BsiHKAI G_wGCw'C
Cuts at: 2759 3424 4202 4392 5186 2759
Size: 665 778 190 794 3985
Fragments arranged by size:
3985 794 778 665 190
BsmI GAATG_Cn'
Cuts at: 2723 2723
Size: 6412
Bsp1286I G_dGCh'C
Cuts at: 937 1687 2029 2759 3424 4035 4128 4202 4392
Size: 750 342 730 665 611 93 74 190
Cuts at: 4392 4454 5186 937
Size: 62 732 2163
Fragments arranged by size:
2163 750 732 730 665 611 342 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5632 5632
Size: 6412
BspHI T'CATG_A
Cuts at: 5588 5588
Size: 6412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2256 3460 3674 3976 4357 2256
Size: 1204 214 302 381 4311
Fragments arranged by size:
4311 1204 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4702 4756 6242 6407 50
Size: 4652 54 1486 165 55
Fragments arranged by size:
4652 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 605 761 1068 2488 4322 605
Size: 156 307 1420 1834 2695
Fragments arranged by size:
2695 1834 1420 307 156
BsrFI r'CCGG_y
Cuts at: 2313 4408 4589 4800 2313
Size: 2095 181 211 3925
Fragments arranged by size:
3925 2095 211 181
BsrGI T'GTAC_A
Cuts at: 454 454
Size: 6412
BssHII G'CGCG_C
Cuts at: 140 140
Size: 6412
BssSI C'ACGA_G
Cuts at: 1784 4681 5041 1784
Size: 2897 360 3155
Fragments arranged by size:
3155 2897 360
BstAPI GCAn_nnn'nTGC
Cuts at: 231 1151 3787 231
Size: 920 2636 2856
Fragments arranged by size:
2856 2636 920
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1441 1758 2675 1441
Size: 317 917 5178
Fragments arranged by size:
5178 917 317
BstYI r'GATC_y
Cuts at: 1 300 2629 2703 3435 3902 3925 4260 4506
Size: 299 2329 74 732 467 23 335 246
Cuts at: 4506 5520 5606 5618 5964 1
Size: 1014 86 12 346 449
Fragments arranged by size:
2329 1014 732 467 449 346 335 299
246 86 74 23 12
Bsu36I CC'TnA_GG
Cuts at: 2399 2399
Size: 6412
BtgI C'CryG_G
Cuts at: 894 1226 2546 2941 3143 3237 3248 894
Size: 332 1320 395 202 94 11 4058
Fragments arranged by size:
4058 1320 395 332 202 94 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4360 4541 4704 4360
Size: 181 163 6068
Fragments arranged by size:
6068 181 163
BtsI GCAGTG_nn'
Cuts at: 582 1274 3653 582
Size: 692 2379 3341
Fragments arranged by size:
3341 2379 692
DraI TTT'AAA
Cuts at: 3409 3409
Size: 6412
DrdI GACnn_nn'nnGTC
Cuts at: 1863 2943 4116 4976 1863
Size: 1080 1173 860 3299
Fragments arranged by size:
3299 1173 1080 860
EaeI y'GGCC_r
Cuts at: 3995 4169 4560 4587 5934 3995
Size: 174 391 27 1347 4473
Fragments arranged by size:
4473 1347 391 174 27
EagI C'GGCC_G
Cuts at: 3995 3995
Size: 6412
EarI CTCTTCn'nnn_
Cuts at: 944 1211 1316 1331 1964 4433 4643 944
Size: 267 105 15 633 2469 210 2713
Fragments arranged by size:
2713 2469 633 267 210 105 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1970 4618 4930 5076 6222 1970
Size: 2648 312 146 1146 2160
Fragments arranged by size:
2648 2160 1146 312 146
EcoICRI GAG'CTC
Cuts at: 2757 3422 2757
Size: 665 5747
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 227 1844 1889 1964 2059 2078 2437 3074
Size: 37 1617 45 75 95 19 359 637
Cuts at: 3074 3352 4235 4667 5416 5684 190
Size: 278 883 432 749 268 918
Fragments arranged by size:
1617 918 883 749 637 432 359 278
268 95 75 45 37 19
EcoNI CCTnn'n_nnAGG
Cuts at: 154 648 154
Size: 494 5918
EcoO109I rG'GnC_Cy
Cuts at: 237 1341 2182 2583 3549 237
Size: 1104 841 401 966 3100
Fragments arranged by size:
3100 1104 966 841 401
EcoRI G'AATT_C
Cuts at: 2590 3414 2590
Size: 824 5588
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4429 4429
Size: 6412
FspI TGC'GCA
Cuts at: 4191 4191
Size: 6412
HaeII r_GCGC'y
Cuts at: 539 3844 4092 5116 6095 539
Size: 3305 248 1024 979 856
Fragments arranged by size:
3305 1024 979 856 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1238 1270 1819 1851 2614 2646 2746 2778 2786
Size: 32 549 32 763 32 100 32 8
Cuts at: 2786 2818 3102 3134 3154 3186 4252 4282 4284
Size: 32 284 32 20 32 1066 30 2
Cuts at: 4284 4314 1238
Size: 30 3336
Fragments arranged by size:
3336 1066 763 549 284 100 32 32
32 32 32 32 32 30 30 20
8 2
HincII GTy'rAC
Cuts at: 3449 3449
Size: 6412
HindIII A'AGCT_T
Cuts at: 986 986
Size: 6412
KasI G'GCGC_C
Cuts at: 3840 4088 3840
Size: 248 6164
KpnI G_GTAC'C
Cuts at: 3430 3430
Size: 6412
MfeI C'AATT_G
Cuts at: 499 499
Size: 6412
MluI A'CGCG_T
Cuts at: 5845 5845
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1087 3061 5083 5267 1087
Size: 1974 2022 184 2232
Fragments arranged by size:
2232 2022 1974 184
MscI TGG'CCA
Cuts at: 4171 4171
Size: 6412
MslI CAynn'nnrTG
Cuts at: 766 899 1049 1331 4399 4526 766
Size: 133 150 282 3068 127 2652
Fragments arranged by size:
3068 2652 282 150 133 127
MspA1I CmG'CkG
Cuts at: 205 787 2047 2262 2385 3339 3521 3835 4195
Size: 582 1260 215 123 954 182 314 360
Cuts at: 4195 5210 5455 205
Size: 1015 245 1162
Fragments arranged by size:
1260 1162 1015 954 582 360 314 245
215 182 123
NaeI GCC'GGC
Cuts at: 2315 4591 2315
Size: 2276 4136
NarI GG'CG_CC
Cuts at: 3841 4089 3841
Size: 248 6164
NcoI C'CATG_G
Cuts at: 1226 2546 3143 3237 3248 1226
Size: 1320 597 94 11 4390
Fragments arranged by size:
4390 1320 597 94 11
NdeI CA'TA_TG
Cuts at: 1992 1992
Size: 6412
NgoMIV G'CCGG_C
Cuts at: 2313 4589 2313
Size: 2276 4136
NruI TCG'CGA
Cuts at: 4773 4773
Size: 6412
NsiI A_TGCA'T
Cuts at: 3314 3314
Size: 6412
NspI r_CATG'y
Cuts at: 658 3463 3963 4872 658
Size: 2805 500 909 2198
Fragments arranged by size:
2805 2198 909 500
PasI CC'CwG_GG
Cuts at: 1250 1687 1250
Size: 437 5975
PciI A'CATG_T
Cuts at: 4868 4868
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1232 1232
Size: 6412
PfoI T'CCnGG_A
Cuts at: 3315 3315
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3409 3409
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 287 319 5551 5583 287
Size: 32 5232 32 1116
Fragments arranged by size:
5232 1116 32 32
PpuMI rG'GwC_Cy
Cuts at: 237 1341 2182 2583 237
Size: 1104 841 401 4066
Fragments arranged by size:
4066 1104 841 401
PsiI TTA'TAA
Cuts at: 692 692
Size: 6412
PstI C_TGCA'G
Cuts at: 137 236 757 1677 2267 3117 3457 4142 137
Size: 99 521 920 590 850 340 685 2407
Fragments arranged by size:
2407 920 850 685 590 521 340 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6412
PvuII CAG'CTG
Cuts at: 205 787 2047 2262 2385 3339 3835 4195 205
Size: 582 1260 215 123 954 496 360 2422
Fragments arranged by size:
2422 1260 954 582 496 360 215 123
RsrII CG'GwC_CG
Cuts at: 4605 4605
Size: 6412
SacI G_AGCT'C
Cuts at: 2759 3424 2759
Size: 665 5747
SalI G'TCGA_C
Cuts at: 3447 3447
Size: 6412
SapI GCTCTTCn'nnn_
Cuts at: 4433 4643 4433
Size: 210 6202
SbfI CC_TGCA'GG
Cuts at: 3457 3457
Size: 6412
ScaI AGT'ACT
Cuts at: 5929 5929
Size: 6412
SexAI A'CCwGG_T
Cuts at: 2105 2105
Size: 6412
SfcI C'TryA_G
Cuts at: 33 133 232 648 753 1165 1508 1673 2081
Size: 100 99 416 105 412 343 165 408
Cuts at: 2081 2263 2610 3113 3453 4138 5133 5324 33
Size: 182 347 503 340 685 995 191 1121
Fragments arranged by size:
1121 995 685 503 416 412 408 347
343 340 191 182 165 105 100 99
SfoI GGC'GCC
Cuts at: 3842 4090 3842
Size: 248 6164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6412
SmaI CCC'GGG
Cuts at: 3432 3432
Size: 6412
SmlI C'TyrA_G
Cuts at: 1347 1904 3565 4480 4974 5236 1347
Size: 557 1661 915 494 262 2523
Fragments arranged by size:
2523 1661 915 557 494 262
SnaBI TAC'GTA
Cuts at: 3402 3402
Size: 6412
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6412
SphI G_CATG'C
Cuts at: 658 3463 3963 658
Size: 2805 500 3107
Fragments arranged by size:
3107 2805 500
StuI AGG'CCT
Cuts at: 880 880
Size: 6412
StyI C'CwwG_G
Cuts at: 597 1226 1537 1803 2546 3143 3237 3248 3544
Size: 629 311 266 743 597 94 11 296
Cuts at: 3544 3898 597
Size: 354 3111
Fragments arranged by size:
3111 743 629 597 354 311 296 266
94 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3136 3993 4126 4585 4796 3136
Size: 857 133 459 211 4752
Fragments arranged by size:
4752 857 459 211 133
TatI w'GTAC_w
Cuts at: 454 1286 5927 454
Size: 832 4641 939
Fragments arranged by size:
4641 939 832
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2093 2125 5929 5961 2093
Size: 32 3804 32 2544
Fragments arranged by size:
3804 2544 32 32
Tth111I GACn'n_nGTC
Cuts at: 4207 4207
Size: 6412
XbaI T'CTAG_A
Cuts at: 2520 3441 2520
Size: 921 5491
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 209 2113 2594 3905 209
Size: 1904 481 1311 2716
Fragments arranged by size:
2716 1904 1311 481
XmaI C'CCGG_G
Cuts at: 3430 3430
Size: 6412
XmnI GAAnn'nnTTC
Cuts at: 1321 1321
Size: 6412
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsgI
BsiWI BsmBI BstXI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI NheI NotI PacI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI