Restriction map for
pF1KSDA0238
(Circular) (Six-base) MAPSORT of: pF1KSDA0238.seq Check: 946 from: 1 to: 5087
pF1KSDA0238 5087 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 2125 2125
Size: 5087
Acc65I G'GTAC_C
Cuts at: 422 2103 422
Size: 1681 3406
AclI AA'CG_TT
Cuts at: 595 4927 595
Size: 4332 755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 450 862 1956 2912 3344 4091 4359 450
Size: 412 1094 956 432 747 268 1178
Fragments arranged by size:
1178 1094 956 747 432 412 268
AflIII A'CryG_T
Cuts at: 2439 3543 4520 2439
Size: 1104 977 3006
Fragments arranged by size:
3006 1104 977
AgeI A'CCGG_T
Cuts at: 3475 3475
Size: 5087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 961 961
Size: 5087
AlwNI CAG_nnn'CTG
Cuts at: 2392 3959 2392
Size: 1567 3520
ApaLI G'TGCA_C
Cuts at: 1328 3857 1328
Size: 2529 2558
ApoI r'AATT_y
Cuts at: 997 1379 1659 2091 4440 4696 997
Size: 382 280 432 2349 256 1388
Fragments arranged by size:
2349 1388 432 382 280 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5087
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
AvaI C'yCGr_G
Cuts at: 653 2107 653
Size: 1454 3633
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 414 447 414
Size: 33 5054
BamHI G'GATC_C
Cuts at: 758 1575 2112 758
Size: 817 537 3733
Fragments arranged by size:
3733 817 537
BanI G'GyrC_C
Cuts at: 250 422 2103 2517 2765 2800 250
Size: 172 1681 414 248 35 2537
Fragments arranged by size:
2537 1681 414 248 172 35
BanII G_rGCy'C
Cuts at: 293 337 837 1593 1709 2101 3131 293
Size: 44 500 756 116 392 1030 2249
Fragments arranged by size:
2249 1030 756 500 392 116 44
BbeI G_GCGC'C
Cuts at: 254 2521 2769 254
Size: 2267 248 2572
Fragments arranged by size:
2572 2267 248
BbsI GAAGACnn'nnnn_
Cuts at: 203 1122 2063 203
Size: 919 941 3227
Fragments arranged by size:
3227 941 919
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 465 499 465
Size: 34 5053
BciVI GTATCCnnnnn_n'
Cuts at: 761 1101 2068 2985 3746 5087 761
Size: 340 967 917 761 1341 761
Fragments arranged by size:
1341 967 917 761 761 340
BclI T'GATC_A
Cuts at: 1616 2607 1616
Size: 991 4096
BglI GCCn_nnn'nGGC
Cuts at: 821 821
Size: 5087
BglII A'GATC_T
Cuts at: 2602 4639 2602
Size: 2037 3050
BlpI GC'TnA_GC
Cuts at: 2199 2199
Size: 5087
Bme1580I G_kGCm'C
Cuts at: 488 1332 1347 2712 2805 3861 488
Size: 844 15 1365 93 1056 1714
Fragments arranged by size:
1714 1365 1056 844 93 15
BmrI ACTGGGnnnn_n'
Cuts at: 942 1436 2299 2380 2715 4859 942
Size: 494 863 81 335 2144 1170
Fragments arranged by size:
2144 1170 863 494 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1534 3454 1534
Size: 1920 3167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 1835 243
Size: 1592 3495
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2263 3142 3634 3932 2263
Size: 879 492 298 3418
Fragments arranged by size:
3418 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 340 4791 340
Size: 4451 636
BsaAI yAC'GTr
Cuts at: 2079 2440 2079
Size: 361 4726
BsaBI GATnn'nnATC
Cuts at: 6 2601 4638 6
Size: 2595 2037 455
Fragments arranged by size:
2595 2037 455
BsaHI Gr'CG_yC
Cuts at: 251 2518 2766 3468 4964 251
Size: 2267 248 702 1496 374
Fragments arranged by size:
2267 1496 702 374 248
BsaWI w'CCGG_w
Cuts at: 231 1572 1868 2276 2797 3475 3749 3896 4307
Size: 1341 296 408 521 678 274 147 411
Cuts at: 4307 231
Size: 1011
Fragments arranged by size:
1341 1011 678 521 411 408 296 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 808 808
Size: 5087
BseYI C'CCAG_C
Cuts at: 213 377 1584 2512 3270 3847 4343 213
Size: 164 1207 928 758 577 496 957
Fragments arranged by size:
1207 957 928 758 577 496 164
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1584 1584
Size: 5087
BsiEI CG_ry'CG
Cuts at: 104 479 1259 2629 2675 3883 104
Size: 375 780 1370 46 1208 1308
Fragments arranged by size:
1370 1308 1208 780 375 46
BsiHKAI G_wGCw'C
Cuts at: 293 337 1332 2101 2879 3069 3861 293
Size: 44 995 769 778 190 792 1519
Fragments arranged by size:
1519 995 792 778 769 190 44
Bsp1286I G_dGCh'C
Cuts at: 293 337 488 837 1332 1347 1593 1709 2101
Size: 44 151 349 495 15 246 116 392
Cuts at: 2101 2712 2805 2879 3069 3131 3861 293
Size: 611 93 74 190 62 730 1519
Fragments arranged by size:
1519 730 611 495 392 349 246 190
151 116 93 74 62 44 15
BspEI T'CCGG_A
Cuts at: 1572 1868 4307 1572
Size: 296 2439 2352
Fragments arranged by size:
2439 2352 296
BspHI T'CATG_A
Cuts at: 4263 4263
Size: 5087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 596 1649 2137 2351 2653 3034 596
Size: 1053 488 214 302 381 2649
Fragments arranged by size:
2649 1053 488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3379 3433 4917 5082 50
Size: 3329 54 1484 165 55
Fragments arranged by size:
3329 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 320 938 2999 320
Size: 618 2061 2408
Fragments arranged by size:
2408 2061 618
BsrFI r'CCGG_y
Cuts at: 1258 3085 3266 3475 1258
Size: 1827 181 209 2870
Fragments arranged by size:
2870 1827 209 181
BssSI C'ACGA_G
Cuts at: 3358 3716 3358
Size: 358 4729
BstAPI GCAn_nnn'nTGC
Cuts at: 1188 2464 1188
Size: 1276 3811
BstBI TT'CG_AA
Cuts at: 110 3448 110
Size: 3338 1749
BstEII G'GTnAC_C
Cuts at: 608 608
Size: 5087
BstXI CCAn_nnnn'nTGG
Cuts at: 162 513 547 1232 162
Size: 351 34 685 4017
Fragments arranged by size:
4017 685 351 34
BstYI r'GATC_y
Cuts at: 1 758 767 1575 2112 2579 2602 2937 3183
Size: 757 9 808 537 467 23 335 246
Cuts at: 3183 4195 4281 4293 4639 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 808 757 537 467 449 346 335
246 86 23 12 9
Bsu36I CC'TnA_GG
Cuts at: 2023 2023
Size: 5087
BtgI C'CryG_G
Cuts at: 872 1204 1283 2069 872
Size: 332 79 786 3890
Fragments arranged by size:
3890 786 332 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3037 3218 3381 3037
Size: 181 163 4743
Fragments arranged by size:
4743 181 163
BtsI GCAGTG_nn'
Cuts at: 488 2330 488
Size: 1842 3245
ClaI AT'CG_AT
Cuts at: 1763 1763
Size: 5087
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1982 2017 1982
Size: 35 5052
DraI TTT'AAA
Cuts at: 1658 2086 1658
Size: 428 4659
DrdI GACnn_nn'nnGTC
Cuts at: 2793 3651 2793
Size: 858 4229
EaeI y'GGCC_r
Cuts at: 1079 1256 2672 2846 3237 3264 4609 1079
Size: 177 1416 174 391 27 1345 1557
Fragments arranged by size:
1557 1416 1345 391 177 174 27
EagI C'GGCC_G
Cuts at: 1256 2672 1256
Size: 1416 3671
EarI CTCTTCn'nnn_
Cuts at: 233 2023 3110 3320 233
Size: 1790 1087 210 2000
Fragments arranged by size:
2000 1790 1087 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3295 3605 3751 4897 3295
Size: 310 146 1146 3485
Fragments arranged by size:
3485 1146 310 146
EcoICRI GAG'CTC
Cuts at: 291 335 2099 291
Size: 44 1764 3279
Fragments arranged by size:
3279 1764 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 450 862 1835 1956 2912 3344 4091 4359
Size: 207 412 973 121 956 432 747 268
Cuts at: 4359 243
Size: 971
Fragments arranged by size:
973 971 956 747 432 412 268 207
121
EcoNI CCTnn'n_nnAGG
Cuts at: 619 619
Size: 5087
EcoO109I rG'GnC_Cy
Cuts at: 2226 2226
Size: 5087
EcoRI G'AATT_C
Cuts at: 2091 2091
Size: 5087
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1173 1207 3106 1173
Size: 34 1899 3154
Fragments arranged by size:
3154 1899 34
FspI TGC'GCA
Cuts at: 2868 2868
Size: 5087
HaeII r_GCGC'y
Cuts at: 254 1556 2521 2769 3791 4770 254
Size: 1302 965 248 1022 979 571
Fragments arranged by size:
1302 1022 979 965 571 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1679 1711 2044 2076 2929 2959 2961 2991 1679
Size: 32 333 32 853 30 2 30 3775
Fragments arranged by size:
3775 853 333 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2126 2126
Size: 5087
KasI G'GCGC_C
Cuts at: 250 2517 2765 250
Size: 2267 248 2572
Fragments arranged by size:
2572 2267 248
KpnI G_GTAC'C
Cuts at: 426 2107 426
Size: 1681 3406
MluI A'CGCG_T
Cuts at: 4520 4520
Size: 5087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3758 3942 3758
Size: 184 4903
MscI TGG'CCA
Cuts at: 1081 2848 1081
Size: 1767 3320
MslI CAynn'nnrTG
Cuts at: 511 784 943 1230 1270 3076 3203 511
Size: 273 159 287 40 1806 127 2395
Fragments arranged by size:
2395 1806 287 273 159 127 40
MspA1I CmG'CkG
Cuts at: 263 470 910 1053 1624 2198 2512 2872 3885
Size: 207 440 143 571 574 314 360 1013
Cuts at: 3885 4130 263
Size: 245 1220
Fragments arranged by size:
1220 1013 574 571 440 360 314 245
207 143
NaeI GCC'GGC
Cuts at: 3268 3268
Size: 5087
NarI GG'CG_CC
Cuts at: 251 2518 2766 251
Size: 2267 248 2572
Fragments arranged by size:
2572 2267 248
NgoMIV G'CCGG_C
Cuts at: 3266 3266
Size: 5087
NspI r_CATG'y
Cuts at: 464 1275 2140 2640 3547 464
Size: 811 865 500 907 2004
Fragments arranged by size:
2004 907 865 811 500
PciI A'CATG_T
Cuts at: 3543 3543
Size: 5087
PfoI T'CCnGG_A
Cuts at: 1666 1826 1666
Size: 160 4927
PmeI GTTT'AAAC
Cuts at: 2086 2086
Size: 5087
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 4226
Size: 32 5055
PshAI GACnn'nnGTC
Cuts at: 1126 1126
Size: 5087
PsiI TTA'TAA
Cuts at: 1016 1954 1016
Size: 938 4149
PspXI vC'TCGA_Gb
Cuts at: 653 653
Size: 5087
PstI C_TGCA'G
Cuts at: 1646 2134 2819 1646
Size: 488 685 3914
Fragments arranged by size:
3914 685 488
PvuI CG_AT'CG
Cuts at: 104 479 104
Size: 375 4712
PvuII CAG'CTG
Cuts at: 1053 1624 2512 2872 1053
Size: 571 888 360 3268
Fragments arranged by size:
3268 888 571 360
RsrII CG'GwC_CG
Cuts at: 3282 3282
Size: 5087
SacI G_AGCT'C
Cuts at: 293 337 2101 293
Size: 44 1764 3279
Fragments arranged by size:
3279 1764 44
SalI G'TCGA_C
Cuts at: 2124 2124
Size: 5087
SapI GCTCTTCn'nnn_
Cuts at: 2023 3110 3320 2023
Size: 1087 210 3790
Fragments arranged by size:
3790 1087 210
SbfI CC_TGCA'GG
Cuts at: 2134 2134
Size: 5087
ScaI AGT'ACT
Cuts at: 4604 4604
Size: 5087
SfcI C'TryA_G
Cuts at: 33 445 586 1642 2130 2815 3808 3999 33
Size: 412 141 1056 488 685 993 191 1121
Fragments arranged by size:
1121 1056 993 685 488 412 191 141
SfiI GGCCn_nnn'nGGCC
Cuts at: 821 821
Size: 5087
SfoI GGC'GCC
Cuts at: 252 2519 2767 252
Size: 2267 248 2572
Fragments arranged by size:
2572 2267 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5087
SmaI CCC'GGG
Cuts at: 2109 2109
Size: 5087
SmlI C'TyrA_G
Cuts at: 653 2242 3157 3649 3911 653
Size: 1589 915 492 262 1829
Fragments arranged by size:
1829 1589 915 492 262
SnaBI TAC'GTA
Cuts at: 2079 2079
Size: 5087
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5087
SphI G_CATG'C
Cuts at: 1275 2140 2640 1275
Size: 865 500 3722
Fragments arranged by size:
3722 865 500
SspI AAT'ATT
Cuts at: 956 956
Size: 5087
StuI AGG'CCT
Cuts at: 1394 2021 1394
Size: 627 4460
StyI C'CwwG_G
Cuts at: 2221 2575 2221
Size: 354 4733
SwaI ATTT'AAAT
Cuts at: 1658 1658
Size: 5087
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 227 841 885 2670 2803 3262 3471 227
Size: 614 44 1785 133 459 209 1843
Fragments arranged by size:
1843 1785 614 459 209 133 44
TatI w'GTAC_w
Cuts at: 845 4602 845
Size: 3757 1330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1370 1402 1869 1901 4604 4636 1370
Size: 32 467 32 2703 32 1821
Fragments arranged by size:
2703 1821 467 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2884 2884
Size: 5087
XbaI T'CTAG_A
Cuts at: 2118 2118
Size: 5087
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2582 2582
Size: 5087
XhoI C'TCGA_G
Cuts at: 653 653
Size: 5087
XmaI C'CCGG_G
Cuts at: 2107 2107
Size: 5087
XmnI GAAnn'nnTTC
Cuts at: 410 410
Size: 5087
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BbvCI BfrBI BmgBI BmtI Bpu10I BsaXI
BsiWI BsmI BsmBI BsrGI BssHII BstZ17I DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PspOMI PsrI SacII SanDI SexAI SgrAI SrfI ZraI