Restriction map for
pF1KSDA0235
(Circular) (Six-base) MAPSORT of: pF1KSDA0235.seq Check: 9829 from: 1 to: 6331
pF1KSDA0235 6331 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AccI GT'mk_AC
Cuts at: 1096 2919 3367 1096
Size: 1823 448 4060
Fragments arranged by size:
4060 1823 448
Acc65I G'GTAC_C
Cuts at: 855 3345 855
Size: 2490 3841
AclI AA'CG_TT
Cuts at: 6171 6171
Size: 6331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 854 2275 2945 4154 4586 5335 5603 698
Size: 156 1421 670 1209 432 749 268 1426
Fragments arranged by size:
1426 1421 1209 749 670 432 268 156
AfeI AGC'GCT
Cuts at: 691 691
Size: 6331
AflIII A'CryG_T
Cuts at: 3681 4787 5764 3681
Size: 1106 977 4248
Fragments arranged by size:
4248 1106 977
AgeI A'CCGG_T
Cuts at: 4719 4719
Size: 6331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 622 992 1226 1693 2017 2828 622
Size: 370 234 467 324 811 4125
Fragments arranged by size:
4125 811 467 370 324 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1490 1522 1490
Size: 32 6299
AlwNI CAG_nnn'CTG
Cuts at: 795 1576 2125 3634 5203 795
Size: 781 549 1509 1569 1923
Fragments arranged by size:
1923 1569 1509 781 549
ApaI G_GGCC'C
Cuts at: 700 700
Size: 6331
ApaLI G'TGCA_C
Cuts at: 5101 5101
Size: 6331
ApoI r'AATT_y
Cuts at: 468 769 2411 2986 3333 5684 5940 468
Size: 301 1642 575 347 2351 256 859
Fragments arranged by size:
2351 1642 859 575 347 301 256
AseI AT'TA_AT
Cuts at: 20 2434 20
Size: 2414 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6331
AvaI C'yCGr_G
Cuts at: 161 3349 161
Size: 3188 3143
AvrII C'CTAG_G
Cuts at: 3282 3282
Size: 6331
BamHI G'GATC_C
Cuts at: 2059 3354 2059
Size: 1295 5036
BanI G'GyrC_C
Cuts at: 855 1459 3345 3759 4007 4042 855
Size: 604 1886 414 248 35 3144
Fragments arranged by size:
3144 1886 604 414 248 35
BanII G_rGCy'C
Cuts at: 700 1503 3343 4373 700
Size: 803 1840 1030 2658
Fragments arranged by size:
2658 1840 1030 803
BbeI G_GCGC'C
Cuts at: 3763 4011 3763
Size: 248 6083
BbsI GAAGACnn'nnnn_
Cuts at: 2933 2933
Size: 6331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3172 3206 3172
Size: 34 6297
BciVI GTATCCnnnnn_n'
Cuts at: 1193 4227 4990 6331 1193
Size: 3034 763 1341 1193
Fragments arranged by size:
3034 1341 1193 763
BclI T'GATC_A
Cuts at: 1449 3849 1449
Size: 2400 3931
BfrBI ATG'CAT
Cuts at: 979 979
Size: 6331
BglI GCCn_nnn'nGGC
Cuts at: 1601 1601
Size: 6331
BglII A'GATC_T
Cuts at: 322 1915 3844 5883 322
Size: 1593 1929 2039 770
Fragments arranged by size:
2039 1929 1593 770
BlpI GC'TnA_GC
Cuts at: 992 1259 1818 2387 3441 992
Size: 267 559 569 1054 3882
Fragments arranged by size:
3882 1054 569 559 267
Bme1580I G_kGCm'C
Cuts at: 700 3954 4047 5105 700
Size: 3254 93 1058 1926
Fragments arranged by size:
3254 1926 1058 93
BmrI ACTGGGnnnn_n'
Cuts at: 733 3541 3622 3957 6103 733
Size: 2808 81 335 2146 961
Fragments arranged by size:
2808 2146 961 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1901 2396 4696 1901
Size: 495 2300 3536
Fragments arranged by size:
3536 2300 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1019 1064 1310 1487 1499 1598 2000 2147 2163
Size: 45 246 177 12 99 402 147 16
Cuts at: 2163 2366 1019
Size: 203 4984
Fragments arranged by size:
4984 402 246 203 177 147 99 45
16 12
Bpu10I CC'TnA_GC
Cuts at: 2541 2541
Size: 6331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 3505 4384 4878 5176 2315
Size: 1190 879 494 298 3470
Fragments arranged by size:
3470 1190 879 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 551 6035 551
Size: 5484 847
BsaAI yAC'GTr
Cuts at: 3129 3321 3682 3129
Size: 192 361 5778
Fragments arranged by size:
5778 361 192
BsaBI GATnn'nnATC
Cuts at: 6 641 2064 3843 5882 6
Size: 635 1423 1779 2039 455
Fragments arranged by size:
2039 1779 1423 635 455
BsaHI Gr'CG_yC
Cuts at: 3760 4008 4712 6208 3760
Size: 248 704 1496 3883
Fragments arranged by size:
3883 1496 704 248
BsaWI w'CCGG_w
Cuts at: 3518 4039 4719 4993 5140 5551 3518
Size: 521 680 274 147 411 4298
Fragments arranged by size:
4298 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 866 896 1265 1295 1490 1520 866
Size: 30 369 30 195 30 5677
Fragments arranged by size:
5677 369 195 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 556 1067 1965 556
Size: 511 898 4922
Fragments arranged by size:
4922 898 511
BseYI C'CCAG_C
Cuts at: 299 905 3754 4512 5091 5587 299
Size: 606 2849 758 579 496 1043
Fragments arranged by size:
2849 1043 758 606 579 496
BsiEI CG_ry'CG
Cuts at: 104 2919 3871 3917 5127 104
Size: 2815 952 46 1210 1308
Fragments arranged by size:
2815 1308 1210 952 46
BsiHKAI G_wGCw'C
Cuts at: 1503 2645 3343 4121 4311 5105 1503
Size: 1142 698 778 190 794 2729
Fragments arranged by size:
2729 1142 794 778 698 190
BsmI GAATG_Cn'
Cuts at: 3317 3317
Size: 6331
Bsp1286I G_dGCh'C
Cuts at: 700 1503 2645 3343 3954 4047 4121 4311 4373
Size: 803 1142 698 611 93 74 190 62
Cuts at: 4373 5105 700
Size: 732 1926
Fragments arranged by size:
1926 1142 803 732 698 611 190 93
74 62
BspEI T'CCGG_A
Cuts at: 5551 5551
Size: 6331
BspHI T'CATG_A
Cuts at: 132 5507 132
Size: 5375 956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1123 1285 3168 3379 3593 3895 4276 1123
Size: 162 1883 211 214 302 381 3178
Fragments arranged by size:
3178 1883 381 302 214 211 162
BsrBI CCG'CTC
Cuts at: 50 4621 4675 6161 6326 50
Size: 4571 54 1486 165 55
Fragments arranged by size:
4571 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2805 3003 4241 2805
Size: 198 1238 4895
Fragments arranged by size:
4895 1238 198
BsrFI r'CCGG_y
Cuts at: 4327 4508 4719 4327
Size: 181 211 5939
Fragments arranged by size:
5939 211 181
BsrGI T'GTAC_A
Cuts at: 1907 2679 1907
Size: 772 5559
BssSI C'ACGA_G
Cuts at: 4600 4960 4600
Size: 360 5971
BstAPI GCAn_nnn'nTGC
Cuts at: 1030 1558 3706 1030
Size: 528 2148 3655
Fragments arranged by size:
3655 2148 528
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6331
BstXI CCAn_nnnn'nTGG
Cuts at: 3076 3129 3076
Size: 53 6278
BstYI r'GATC_y
Cuts at: 1 322 1915 2059 3354 3821 3844 4179 4425
Size: 321 1593 144 1295 467 23 335 246
Cuts at: 4425 5439 5525 5537 5883 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1593 1295 1014 467 449 346 335 321
246 144 86 23 12
BstZ17I GTA'TAC
Cuts at: 1097 1097
Size: 6331
BtgI C'CryG_G
Cuts at: 803 1171 803
Size: 368 5963
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4279 4460 4623 4279
Size: 181 163 5987
Fragments arranged by size:
5987 181 163
BtsI GCAGTG_nn'
Cuts at: 1148 2810 3572 1148
Size: 1662 762 3907
Fragments arranged by size:
3907 1662 762
DraI TTT'AAA
Cuts at: 3328 3328
Size: 6331
DrdI GACnn_nn'nnGTC
Cuts at: 4035 4895 4035
Size: 860 5471
EaeI y'GGCC_r
Cuts at: 1624 2241 3242 3914 4088 4479 4506 5853 1624
Size: 617 1001 672 174 391 27 1347 2102
Fragments arranged by size:
2102 1347 1001 672 617 391 174 27
EagI C'GGCC_G
Cuts at: 3914 3914
Size: 6331
EarI CTCTTCn'nnn_
Cuts at: 737 873 4352 4562 737
Size: 136 3479 210 2506
Fragments arranged by size:
3479 2506 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4537 4849 4995 6141 4537
Size: 312 146 1146 4727
Fragments arranged by size:
4727 1146 312 146
EcoICRI GAG'CTC
Cuts at: 1501 3341 1501
Size: 1840 4491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 854 1019 1064 1310 1487 1499 1598 2000
Size: 156 165 45 246 177 12 99 402
Cuts at: 2000 2147 2163 2275 2366 2945 4154 4586 5335
Size: 147 16 112 91 579 1209 432 749
Cuts at: 5335 5603 698
Size: 268 1426
Fragments arranged by size:
1426 1209 749 579 432 402 268 246
177 165 156 147 112 99 91 45
16 12
EcoNI CCTnn'n_nnAGG
Cuts at: 506 3293 506
Size: 2787 3544
EcoO109I rG'GnC_Cy
Cuts at: 697 727 3286 3468 697
Size: 30 2559 182 3560
Fragments arranged by size:
3560 2559 182 30
EcoRI G'AATT_C
Cuts at: 3333 3333
Size: 6331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1724 4348 1724
Size: 2624 3707
FspI TGC'GCA
Cuts at: 3214 4110 3214
Size: 896 5435
HaeII r_GCGC'y
Cuts at: 693 3763 4011 5035 6014 693
Size: 3070 248 1024 979 1010
Fragments arranged by size:
3070 1024 1010 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1873 1905 2478 2510 3180 3212 4171 4201 4203
Size: 32 573 32 670 32 959 30 2
Cuts at: 4203 4233 1873
Size: 30 3971
Fragments arranged by size:
3971 959 670 573 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 953 1759 2920 2980 3368 953
Size: 806 1161 60 388 3916
Fragments arranged by size:
3916 1161 806 388 60
HpaI GTT'AAC
Cuts at: 953 953
Size: 6331
KasI G'GCGC_C
Cuts at: 3759 4007 3759
Size: 248 6083
KpnI G_GTAC'C
Cuts at: 859 3349 859
Size: 2490 3841
MfeI C'AATT_G
Cuts at: 1628 1628
Size: 6331
MluI A'CGCG_T
Cuts at: 5764 5764
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 697 1399 1459 1546 3230 5002 5186 697
Size: 702 60 87 1684 1772 184 1842
Fragments arranged by size:
1842 1772 1684 702 184 87 60
MscI TGG'CCA
Cuts at: 3244 4090 3244
Size: 846 5485
MslI CAynn'nnrTG
Cuts at: 2771 3103 4318 4445 2771
Size: 332 1215 127 4657
Fragments arranged by size:
4657 1215 332 127
MspA1I CmG'CkG
Cuts at: 1039 1216 1360 1576 2386 3440 3754 4114 5129
Size: 177 144 216 810 1054 314 360 1015
Cuts at: 5129 5374 1039
Size: 245 1996
Fragments arranged by size:
1996 1054 1015 810 360 314 245 216
177 144
NaeI GCC'GGC
Cuts at: 4510 4510
Size: 6331
NarI GG'CG_CC
Cuts at: 3760 4008 3760
Size: 248 6083
NcoI C'CATG_G
Cuts at: 803 1171 803
Size: 368 5963
NgoMIV G'CCGG_C
Cuts at: 4508 4508
Size: 6331
NruI TCG'CGA
Cuts at: 4692 4692
Size: 6331
NsiI A_TGCA'T
Cuts at: 981 981
Size: 6331
NspI r_CATG'y
Cuts at: 1413 1998 2905 3382 3882 4791 1413
Size: 585 907 477 500 909 2953
Fragments arranged by size:
2953 909 907 585 500 477
PciI A'CATG_T
Cuts at: 4787 4787
Size: 6331
PflMI CCAn_nnn'nTGG
Cuts at: 3307 3307
Size: 6331
PfoI T'CCnGG_A
Cuts at: 651 2016 651
Size: 1365 4966
PmeI GTTT'AAAC
Cuts at: 3328 3328
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1490 1522 5470 5502 1490
Size: 32 3948 32 2319
Fragments arranged by size:
3948 2319 32 32
PpuMI rG'GwC_Cy
Cuts at: 3286 3286
Size: 6331
PspOMI G'GGCC_C
Cuts at: 696 696
Size: 6331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2810 2842 2810
Size: 32 6299
PstI C_TGCA'G
Cuts at: 960 1104 1119 1215 1365 1563 2121 2782 2817
Size: 144 15 96 150 198 558 661 35
Cuts at: 2817 3376 4061 960
Size: 559 685 3230
Fragments arranged by size:
3230 685 661 559 558 198 150 144
96 35 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6331
PvuII CAG'CTG
Cuts at: 1039 1216 1360 1576 2386 3754 4114 1039
Size: 177 144 216 810 1368 360 3256
Fragments arranged by size:
3256 1368 810 360 216 177 144
RsrII CG'GwC_CG
Cuts at: 4524 4524
Size: 6331
SacI G_AGCT'C
Cuts at: 1503 3343 1503
Size: 1840 4491
SalI G'TCGA_C
Cuts at: 2918 3366 2918
Size: 448 5883
SapI GCTCTTCn'nnn_
Cuts at: 4352 4562 4352
Size: 210 6121
SbfI CC_TGCA'GG
Cuts at: 3376 3376
Size: 6331
ScaI AGT'ACT
Cuts at: 1427 5848 1427
Size: 4421 1910
SexAI A'CCwGG_T
Cuts at: 1752 1752
Size: 6331
SfcI C'TryA_G
Cuts at: 33 743 881 956 1100 1115 1211 1361 1559
Size: 710 138 75 144 15 96 150 198
Cuts at: 1559 1811 2117 2716 2778 2813 3372 4057 5052
Size: 252 306 599 62 35 559 685 995
Cuts at: 5052 5243 33
Size: 191 1121
Fragments arranged by size:
1121 995 710 685 599 559 306 252
198 191 150 144 138 96 75 62
35 15
SfoI GGC'GCC
Cuts at: 3761 4009 3761
Size: 248 6083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6331
SmaI CCC'GGG
Cuts at: 3351 3351
Size: 6331
SmlI C'TyrA_G
Cuts at: 2330 3484 4399 4893 5155 2330
Size: 1154 915 494 262 3506
Fragments arranged by size:
3506 1154 915 494 262
SnaBI TAC'GTA
Cuts at: 3321 3321
Size: 6331
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6331
SphI G_CATG'C
Cuts at: 1413 1998 3382 3882 1413
Size: 585 1384 500 3862
Fragments arranged by size:
3862 1384 585 500
StuI AGG'CCT
Cuts at: 392 887 1028 392
Size: 495 141 5695
Fragments arranged by size:
5695 495 141
StyI C'CwwG_G
Cuts at: 393 803 1171 1494 3282 3463 3817 393
Size: 410 368 323 1788 181 354 2907
Fragments arranged by size:
2907 1788 410 368 354 323 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1102 1792 1950 3912 4045 4504 4715 1102
Size: 690 158 1962 133 459 211 2718
Fragments arranged by size:
2718 1962 690 459 211 158 133
TatI w'GTAC_w
Cuts at: 1425 1768 1907 2679 2904 5846 1425
Size: 343 139 772 225 2942 1910
Fragments arranged by size:
2942 1910 772 343 225 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5848 5880 5848
Size: 32 6299
Tth111I GACn'n_nGTC
Cuts at: 1754 4126 1754
Size: 2372 3959
XbaI T'CTAG_A
Cuts at: 2347 3360 2347
Size: 1013 5318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3824 3824
Size: 6331
XmaI C'CCGG_G
Cuts at: 3349 3349
Size: 6331
XmnI GAAnn'nnTTC
Cuts at: 1704 2151 2699 1704
Size: 447 548 5336
Fragments arranged by size:
5336 548 447
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AscI BaeI BbvCI
BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII NdeI
NheI NotI PacI PasI PmlI PshAI PsiI PspXI
SacII SanDI SfiI SgrAI SrfI SspI SwaI XhoI
ZraI